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The map label for this gene is purC

Identifier: 158333780

GI number: 158333780

Start: 576278

End: 577015

Strand: Reverse

Name: purC

Synonym: AM1_0588

Alternate gene names: 158333780

Gene position: 577015-576278 (Counterclockwise)

Preceding gene: 158333781

Following gene: 158333779

Centisome position: 8.87

GC content: 49.32

Gene sequence:

>738_bases
ATGTCTACTGGCGAGTTTCTCTACGAAGGGAAAGCCAAAATTATTTATCGGACGGACGATCCAGACATCCTTTTAGCCCA
GTACAAGGATGATGCGACTGCCTTTAATGCTCAAAAACGCGGCACAATCACCAACAAAGGCCGCATCAATTGCGCCATTG
CCACTCACCTATTCAAGGTTCTGGAGGCAGCGGGGATTGCAACCCATTTTATCGATCAGCCAAACCCAGACCAGATGCGA
ATGCGAGCGGTACAAATTGTGCCCCTAGAAGTCGTCGTGCGGAACATTGCAGCTGGGAGTCTATGTCAACAGACAGGACT
AGCGTTAGGTGTTCCCTTGTCACAACCCTTAGTTGATTTCTATTACAAAGATGATGCCCTGGGGGATCCGTTACTCACCC
GTGATCGCATTTTTCTTCTGGAGCTGACAACGCCAGAGCAGTTAGAGCAACTGCGTCAACTGGCCTTGCGAATCAACCAA
ATCCTGATTAATTTTTTCCATCAATGTGGCATTACCCTCGTAGATTTCAAATTGGAATTTGGGATGACGTCGGACCAACA
GCTTTTGTTAGCGGACGAAATCAGCCCCGATACCTGTAGACTCTGGGACGATGCTGAATCAGATCCCACGCGACGGGTCA
TGGATAAAGATCGGTTCCGTCGTGATTTAGGAGAAGTGGATACGGCCTATGCTCGGGTCATGGAGCGGGTTCTAGCCCAG
GACATATACGTCCCCTAG

Upstream 100 bases:

>100_bases
TGTCACTCCCAAAGCATTGACTTTGACCCGGATCCCATAGAATCTTTGCAGCTCATCTCTACTGCCCCACCCCCTGAATA
CCTGAATTAAGGAGACTGTG

Downstream 100 bases:

>100_bases
CTTGGTGCGATAAAATACTCCCTGTTTTGGATTTTTTTAAGGTTTCTCTTAGTACTCATTGTTGTTCAGTTGCATGTGGT
GTGTGAAGTGACTCAATCTC

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSTGEFLYEGKAKIIYRTDDPDILLAQYKDDATAFNAQKRGTITNKGRINCAIATHLFKVLEAAGIATHFIDQPNPDQMR
MRAVQIVPLEVVVRNIAAGSLCQQTGLALGVPLSQPLVDFYYKDDALGDPLLTRDRIFLLELTTPEQLEQLRQLALRINQ
ILINFFHQCGITLVDFKLEFGMTSDQQLLLADEISPDTCRLWDDAESDPTRRVMDKDRFRRDLGEVDTAYARVMERVLAQ
DIYVP

Sequences:

>Translated_245_residues
MSTGEFLYEGKAKIIYRTDDPDILLAQYKDDATAFNAQKRGTITNKGRINCAIATHLFKVLEAAGIATHFIDQPNPDQMR
MRAVQIVPLEVVVRNIAAGSLCQQTGLALGVPLSQPLVDFYYKDDALGDPLLTRDRIFLLELTTPEQLEQLRQLALRINQ
ILINFFHQCGITLVDFKLEFGMTSDQQLLLADEISPDTCRLWDDAESDPTRRVMDKDRFRRDLGEVDTAYARVMERVLAQ
DIYVP
>Mature_244_residues
STGEFLYEGKAKIIYRTDDPDILLAQYKDDATAFNAQKRGTITNKGRINCAIATHLFKVLEAAGIATHFIDQPNPDQMRM
RAVQIVPLEVVVRNIAAGSLCQQTGLALGVPLSQPLVDFYYKDDALGDPLLTRDRIFLLELTTPEQLEQLRQLALRINQI
LINFFHQCGITLVDFKLEFGMTSDQQLLLADEISPDTCRLWDDAESDPTRRVMDKDRFRRDLGEVDTAYARVMERVLAQD
IYVP

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=235, Percent_Identity=31.4893617021277, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI5453539, Length=235, Percent_Identity=31.4893617021277, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI119220559, Length=242, Percent_Identity=30.1652892561983, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788820, Length=234, Percent_Identity=36.3247863247863, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531275, Length=230, Percent_Identity=30, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18860083, Length=234, Percent_Identity=28.6324786324786, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24583917, Length=249, Percent_Identity=28.9156626506024, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_ACAM1 (B0CD47)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514952.1
- ProteinModelPortal:   B0CD47
- SMR:   B0CD47
- GeneID:   5679416
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0588
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- ProtClustDB:   PRK09362
- BioCyc:   AMAR329726:AM1_0588-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 27794; Mature: 27663

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGEFLYEGKAKIIYRTDDPDILLAQYKDDATAFNAQKRGTITNKGRINCAIATHLFKV
CCCCCEEEECCEEEEEECCCCCEEEEEECCCCHHCCCHHCCCCCCCCEEEHHHHHHHHHH
LEAAGIATHFIDQPNPDQMRMRAVQIVPLEVVVRNIAAGSLCQQTGLALGVPLSQPLVDF
HHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHH
YYKDDALGDPLLTRDRIFLLELTTPEQLEQLRQLALRINQILINFFHQCGITLVDFKLEF
HCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
GMTSDQQLLLADEISPDTCRLWDDAESDPTRRVMDKDRFRRDLGEVDTAYARVMERVLAQ
CCCCCCEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYVP
HCCCC
>Mature Secondary Structure 
STGEFLYEGKAKIIYRTDDPDILLAQYKDDATAFNAQKRGTITNKGRINCAIATHLFKV
CCCCEEEECCEEEEEECCCCCEEEEEECCCCHHCCCHHCCCCCCCCEEEHHHHHHHHHH
LEAAGIATHFIDQPNPDQMRMRAVQIVPLEVVVRNIAAGSLCQQTGLALGVPLSQPLVDF
HHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHH
YYKDDALGDPLLTRDRIFLLELTTPEQLEQLRQLALRINQILINFFHQCGITLVDFKLEF
HCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEC
GMTSDQQLLLADEISPDTCRLWDDAESDPTRRVMDKDRFRRDLGEVDTAYARVMERVLAQ
CCCCCCEEEEECCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYVP
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA