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The map label for this gene is hemL

Identifier: 158333741

GI number: 158333741

Start: 533561

End: 534859

Strand: Reverse

Name: hemL

Synonym: AM1_0549

Alternate gene names: 158333741

Gene position: 534859-533561 (Counterclockwise)

Preceding gene: 158333742

Following gene: 158333740

Centisome position: 8.22

GC content: 50.35

Gene sequence:

>1299_bases
TTGCTTACTGACACCTTAAATACAACAAAGTCAGAGGAGATTTTTGCGGCAGCGCAAAAATTGATGCCTGGTGGCGTAAG
TTCACCGGTCCGCGCATTCAAATCTGTGGGCGGCCAACCCATTGTTTTTGATCGGGTTAAAGGGGCTCATGTATGGGATG
TTGATGGCAATCAATATATTGATTACGTCGGCACCTGGGGACCCGCAATTTGTGGTCATGCCCATCCTGAAGTCCTTACG
GCTCTCAAGGAAGCCTTAGACAAAGGCACTAGCTTTGGCGCTCCCTGCTTGTTGGAAAACGTACTAGCTGAAATGGTCAT
TGATGCCGTTCCCAGCGTGGAAGTGGTCCGCTTTGTGAACTCAGGAACTGAAGCTTGTATGTCCGTCCTGAGACTGATGA
GAGCCTTCACTGGCCGGGAAAAAGTGATCAAATTCGAAGGCTGCTATCACGGCCATGCTGATATGTTTTTGGTCAAGGCT
GGCTCTGGTGTGGCAACTTTGGGGCTACCGGATTCGCCCGGTGTGCCCAAATCCGTAACGGGAAATACCCTAACGGCTCC
CTACAACGACTTACAGTCCGTACAAGCCCTATTCTCCCAGCACCCCGATCAGATTGCTGGGGTGATCTTAGAACCAGTCG
TCGGTAATGCTGGGTTTATTCCACCAGATGCCGGGTTCTTAGAAGGCCTCCGAGAAATTACCAAAGAGAATGGCGCTTTA
CTGGTTTTTGACGAAGTCATGACCGGCTTTCGCATCTCCTATGGCGGCGCTCAAGAGAAGTTTGGCATTACCCCCGATTT
AACGACCTTAGGTAAAGTCATTGGGGGCGGCCTGCCCGTGGGTGCATATGGCGGACGTCGAGACATTATGAGTATGGTTG
CTCCGGCAGGCCCCATGTATCAAGCGGGTACGTTGTCCGGTAATCCCTTGGCGATGACAGCAGGAATCAAAACATTAGAA
TTGCTCAGACGTTCAGGAACTTACGATCAATTAGATCGGGTGACCGGCAAGTTAATCTCTGGATTGTTGCAGATTGCTCG
GGAAGCAGGCCATGAGGTGTGTGGGGGGCATATCAACGGCATGTTTGGCATGTTCTTTGCTCCTGGCCCGGTCCATAGCT
ATGACGATGCGAAGGCCGCTGATGCAGCTAAGTTCGCTAAATTCCATCGGGGAATGTTGGAACAAGGGGTTTATTTAGCG
CCTTCCCAATTTGAGGCAGGTTTTACCTCCTTAGCTCATACTGATGCAGATATCGACCAAACCTTAGAAGCAGCCAAAGT
TGTATTGGCTAATATCTAA

Upstream 100 bases:

>100_bases
CTATGTTGATAGGGCTTAAGCATGAATTTGCTGAGTGCACCTTTCAAAGGCCTTGATCATTCAACCATTCAGCGAGTCAG
CTGATCTTAAGGAGAAAACG

Downstream 100 bases:

>100_bases
TCTTTAATTGAGAAGTTGATTTCATCAGCGAAATACTATCTTTAAAAAATCATTCAAGAGAGGCCTATAGGCCTCTCTTT
TTTATGAATGTCAACCATAA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLTDTLNTTKSEEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGAHVWDVDGNQYIDYVGTWGPAICGHAHPEVLT
ALKEALDKGTSFGAPCLLENVLAEMVIDAVPSVEVVRFVNSGTEACMSVLRLMRAFTGREKVIKFEGCYHGHADMFLVKA
GSGVATLGLPDSPGVPKSVTGNTLTAPYNDLQSVQALFSQHPDQIAGVILEPVVGNAGFIPPDAGFLEGLREITKENGAL
LVFDEVMTGFRISYGGAQEKFGITPDLTTLGKVIGGGLPVGAYGGRRDIMSMVAPAGPMYQAGTLSGNPLAMTAGIKTLE
LLRRSGTYDQLDRVTGKLISGLLQIAREAGHEVCGGHINGMFGMFFAPGPVHSYDDAKAADAAKFAKFHRGMLEQGVYLA
PSQFEAGFTSLAHTDADIDQTLEAAKVVLANI

Sequences:

>Translated_432_residues
MLTDTLNTTKSEEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGAHVWDVDGNQYIDYVGTWGPAICGHAHPEVLT
ALKEALDKGTSFGAPCLLENVLAEMVIDAVPSVEVVRFVNSGTEACMSVLRLMRAFTGREKVIKFEGCYHGHADMFLVKA
GSGVATLGLPDSPGVPKSVTGNTLTAPYNDLQSVQALFSQHPDQIAGVILEPVVGNAGFIPPDAGFLEGLREITKENGAL
LVFDEVMTGFRISYGGAQEKFGITPDLTTLGKVIGGGLPVGAYGGRRDIMSMVAPAGPMYQAGTLSGNPLAMTAGIKTLE
LLRRSGTYDQLDRVTGKLISGLLQIAREAGHEVCGGHINGMFGMFFAPGPVHSYDDAKAADAAKFAKFHRGMLEQGVYLA
PSQFEAGFTSLAHTDADIDQTLEAAKVVLANI
>Mature_432_residues
MLTDTLNTTKSEEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGAHVWDVDGNQYIDYVGTWGPAICGHAHPEVLT
ALKEALDKGTSFGAPCLLENVLAEMVIDAVPSVEVVRFVNSGTEACMSVLRLMRAFTGREKVIKFEGCYHGHADMFLVKA
GSGVATLGLPDSPGVPKSVTGNTLTAPYNDLQSVQALFSQHPDQIAGVILEPVVGNAGFIPPDAGFLEGLREITKENGAL
LVFDEVMTGFRISYGGAQEKFGITPDLTTLGKVIGGGLPVGAYGGRRDIMSMVAPAGPMYQAGTLSGNPLAMTAGIKTLE
LLRRSGTYDQLDRVTGKLISGLLQIAREAGHEVCGGHINGMFGMFFAPGPVHSYDDAKAADAAKFAKFHRGMLEQGVYLA
PSQFEAGFTSLAHTDADIDQTLEAAKVVLANI

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=312, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24119277, Length=348, Percent_Identity=26.1494252873563, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI37574042, Length=349, Percent_Identity=27.7936962750716, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI13994255, Length=331, Percent_Identity=25.0755287009063, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI226442705, Length=348, Percent_Identity=27.0114942528736, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI226442709, Length=317, Percent_Identity=27.7602523659306, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI284507298, Length=178, Percent_Identity=32.5842696629214, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=55.2941176470588, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789016, Length=363, Percent_Identity=32.5068870523416, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788044, Length=292, Percent_Identity=35.6164383561644, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789759, Length=317, Percent_Identity=31.5457413249211, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693181, Length=307, Percent_Identity=29.9674267100977, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787560, Length=312, Percent_Identity=28.525641025641, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786991, Length=370, Percent_Identity=26.7567567567568, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=351, Percent_Identity=27.6353276353276, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71992977, Length=393, Percent_Identity=26.2086513994911, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=321, Percent_Identity=28.3489096573209, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=362, Percent_Identity=24.0331491712707, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357415, Length=292, Percent_Identity=27.0547945205479, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356575, Length=346, Percent_Identity=27.7456647398844, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_ACAM1 (B0CC57)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514913.1
- ProteinModelPortal:   B0CC57
- SMR:   B0CC57
- GeneID:   5679377
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0549
- HOGENOM:   HBG725944
- OMA:   MNHERSR
- ProtClustDB:   PRK00062
- BioCyc:   AMAR329726:AM1_0549-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45618; Mature: 45618

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDTLNTTKSEEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGAHVWDVDGNQYI
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCCEE
DYVGTWGPAICGHAHPEVLTALKEALDKGTSFGAPCLLENVLAEMVIDAVPSVEVVRFVN
EECCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
SGTEACMSVLRLMRAFTGREKVIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPKSVT
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCC
GNTLTAPYNDLQSVQALFSQHPDQIAGVILEPVVGNAGFIPPDAGFLEGLREITKENGAL
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
LVFDEVMTGFRISYGGAQEKFGITPDLTTLGKVIGGGLPVGAYGGRRDIMSMVAPAGPMY
EEEHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCC
QAGTLSGNPLAMTAGIKTLELLRRSGTYDQLDRVTGKLISGLLQIAREAGHEVCGGHING
CCCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHCC
MFGMFFAPGPVHSYDDAKAADAAKFAKFHRGMLEQGVYLAPSQFEAGFTSLAHTDADIDQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCHHH
TLEAAKVVLANI
HHHHHHHHHHCC
>Mature Secondary Structure
MLTDTLNTTKSEEIFAAAQKLMPGGVSSPVRAFKSVGGQPIVFDRVKGAHVWDVDGNQYI
CCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEECCCCCEE
DYVGTWGPAICGHAHPEVLTALKEALDKGTSFGAPCLLENVLAEMVIDAVPSVEVVRFVN
EECCCCCCHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
SGTEACMSVLRLMRAFTGREKVIKFEGCYHGHADMFLVKAGSGVATLGLPDSPGVPKSVT
CCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCC
GNTLTAPYNDLQSVQALFSQHPDQIAGVILEPVVGNAGFIPPDAGFLEGLREITKENGAL
CCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCE
LVFDEVMTGFRISYGGAQEKFGITPDLTTLGKVIGGGLPVGAYGGRRDIMSMVAPAGPMY
EEEHHHHHCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCC
QAGTLSGNPLAMTAGIKTLELLRRSGTYDQLDRVTGKLISGLLQIAREAGHEVCGGHING
CCCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHCCCHHCC
MFGMFFAPGPVHSYDDAKAADAAKFAKFHRGMLEQGVYLAPSQFEAGFTSLAHTDADIDQ
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCHHH
TLEAAKVVLANI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA