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The map label for this gene is ackA

Identifier: 158333643

GI number: 158333643

Start: 426544

End: 427740

Strand: Direct

Name: ackA

Synonym: AM1_0445

Alternate gene names: 158333643

Gene position: 426544-427740 (Clockwise)

Preceding gene: 158333642

Following gene: 158333650

Centisome position: 6.56

GC content: 48.29

Gene sequence:

>1197_bases
ATGAAGATTTTAGTTCTCAATGCCGGTTCTAGCAGCCACAAATGTTGCCTTTACGCGATTGAAGGGCCTCTCCCCGACCA
TCCAGCACCTCCGCTTTGGGAAGCTCAGCTGGATTGGCATGACCCCAATCATGCCTCGTTAAAAGTCAAAACCAAACGAG
AAAATCTGGAGGAAGACCTATCTGATGTATCACGCGCCGACGCGCTCTCTCATCTTTTAACCACCCTATGGCATGGGCCT
ACCCAAGTCATCCAAATGGCACAGGAGATTAATGTTGTCGGTCATCGTGTGGTGCATGGGGGACAAGACTATCAGTCCAG
TGTGGTGGTGACATCGGAGGTTAAAACTGCGATCGCAGATCTGATTCCTTTAGCCCCCAACCATAATCCAGCCAATTTAG
AAGGCATTAAATTGATCGAACAATTGTTAGGAAATGTTCGCCAAGTAGCTGTCTTCGATACTGCCTTTCACAGTCATCTT
CCCGAAGTTGCGGCCATCTATCCCGGTCCCTATGATTGGCAACAACAAGGCATTCGTCGGTATGGGTTTCATGGCATTAG
TCATCAATATTGCACCCAGCGAACGGCAGAAATTCTGGGCCGAGGGGTGGACCGCCTGATTATTTGTCATTTGGGAAATG
GAGCATCTCTGACAGCGGTTAAACAAGGTCAGAGTATCGACACCTCGATGGGCTTCACCCCCTTAGAAGGATTGATGATG
GGTACGCGTTCAGGGTCGATTGATCCAGGCATTTTAATTCATCTGATGCGGCAAGGATATGATGCCGATCAGCTAGATCA
CATGCTTAATAAAGCCTCTGGCTTAAAGGGGATCTCAGGGGTCTCCCATGATCTGCGGGAGATTGAGCAGGCCATTGCCC
AAGACAATGCCCAAGCTAAGCTAGCGCGAGACTTATATCTCCATCGATTGAAAGCTTGTTTGGGGTCCATGCTGATGAGC
TTAGGTGGAGTTGATGTCTTAGTGTTTACGGGTGGTATAGGGGAGCATTCTGCTTCAGTGAGAGCTGAGACCTGTGAAGC
ACTGGCTTTTTTGGGCCTTAAGCTTGATCCGATTCTGAATCGAAAGTCTCCTGTAGATCAGGATATTGCAGCAGTTGACT
CTAAGGTGCGGGTATTAGTGATTAAGACCCAGGAAGATTGGGCCATTGCTCAATCTTGTTATGAACAGTGTTGTTAG

Upstream 100 bases:

>100_bases
TATGGCCACCCTTCGCAAACTCCGGGTTTCCGAGATTGAAGCGGAGTAGTCTCGTCCTCAAGCCCTGTTTGCTCGTTACC
AGCCATCTGTGGGCCAGATC

Downstream 100 bases:

>100_bases
GGCTGCAGAGGAATGTTGCCGATGCTAGAGAGCAAGATGAGAGGGGTTGCGATGGCGAAAGGCTGCTCTTTGAGCATCGC
AACTGCAATACCATCCCCTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKILVLNAGSSSHKCCLYAIEGPLPDHPAPPLWEAQLDWHDPNHASLKVKTKRENLEEDLSDVSRADALSHLLTTLWHGP
TQVIQMAQEINVVGHRVVHGGQDYQSSVVVTSEVKTAIADLIPLAPNHNPANLEGIKLIEQLLGNVRQVAVFDTAFHSHL
PEVAAIYPGPYDWQQQGIRRYGFHGISHQYCTQRTAEILGRGVDRLIICHLGNGASLTAVKQGQSIDTSMGFTPLEGLMM
GTRSGSIDPGILIHLMRQGYDADQLDHMLNKASGLKGISGVSHDLREIEQAIAQDNAQAKLARDLYLHRLKACLGSMLMS
LGGVDVLVFTGGIGEHSASVRAETCEALAFLGLKLDPILNRKSPVDQDIAAVDSKVRVLVIKTQEDWAIAQSCYEQCC

Sequences:

>Translated_398_residues
MKILVLNAGSSSHKCCLYAIEGPLPDHPAPPLWEAQLDWHDPNHASLKVKTKRENLEEDLSDVSRADALSHLLTTLWHGP
TQVIQMAQEINVVGHRVVHGGQDYQSSVVVTSEVKTAIADLIPLAPNHNPANLEGIKLIEQLLGNVRQVAVFDTAFHSHL
PEVAAIYPGPYDWQQQGIRRYGFHGISHQYCTQRTAEILGRGVDRLIICHLGNGASLTAVKQGQSIDTSMGFTPLEGLMM
GTRSGSIDPGILIHLMRQGYDADQLDHMLNKASGLKGISGVSHDLREIEQAIAQDNAQAKLARDLYLHRLKACLGSMLMS
LGGVDVLVFTGGIGEHSASVRAETCEALAFLGLKLDPILNRKSPVDQDIAAVDSKVRVLVIKTQEDWAIAQSCYEQCC
>Mature_398_residues
MKILVLNAGSSSHKCCLYAIEGPLPDHPAPPLWEAQLDWHDPNHASLKVKTKRENLEEDLSDVSRADALSHLLTTLWHGP
TQVIQMAQEINVVGHRVVHGGQDYQSSVVVTSEVKTAIADLIPLAPNHNPANLEGIKLIEQLLGNVRQVAVFDTAFHSHL
PEVAAIYPGPYDWQQQGIRRYGFHGISHQYCTQRTAEILGRGVDRLIICHLGNGASLTAVKQGQSIDTSMGFTPLEGLMM
GTRSGSIDPGILIHLMRQGYDADQLDHMLNKASGLKGISGVSHDLREIEQAIAQDNAQAKLARDLYLHRLKACLGSMLMS
LGGVDVLVFTGGIGEHSASVRAETCEALAFLGLKLDPILNRKSPVDQDIAAVDSKVRVLVIKTQEDWAIAQSCYEQCC

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=40.5, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI145693184, Length=312, Percent_Identity=43.2692307692308, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_ACAM1 (B0CB11)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514815.1
- ProteinModelPortal:   B0CB11
- SMR:   B0CB11
- GeneID:   5679276
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0445
- HOGENOM:   HBG285863
- OMA:   GGELYSE
- ProtClustDB:   PRK00180
- BioCyc:   AMAR329726:AM1_0445-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43410; Mature: 43410

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVLNAGSSSHKCCLYAIEGPLPDHPAPPLWEAQLDWHDPNHASLKVKTKRENLEEDL
CEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCEEEEEHHHHHHHHHH
SDVSRADALSHLLTTLWHGPTQVIQMAQEINVVGHRVVHGGQDYQSSVVVTSEVKTAIAD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHH
LIPLAPNHNPANLEGIKLIEQLLGNVRQVAVFDTAFHSHLPEVAAIYPGPYDWQQQGIRR
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHH
YGFHGISHQYCTQRTAEILGRGVDRLIICHLGNGASLTAVKQGQSIDTSMGFTPLEGLMM
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHE
GTRSGSIDPGILIHLMRQGYDADQLDHMLNKASGLKGISGVSHDLREIEQAIAQDNAQAK
ECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHH
LARDLYLHRLKACLGSMLMSLGGVDVLVFTGGIGEHSASVRAETCEALAFLGLKLDPILN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCC
RKSPVDQDIAAVDSKVRVLVIKTQEDWAIAQSCYEQCC
CCCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKILVLNAGSSSHKCCLYAIEGPLPDHPAPPLWEAQLDWHDPNHASLKVKTKRENLEEDL
CEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEECCCCCCCCEEEEEHHHHHHHHHH
SDVSRADALSHLLTTLWHGPTQVIQMAQEINVVGHRVVHGGQDYQSSVVVTSEVKTAIAD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHH
LIPLAPNHNPANLEGIKLIEQLLGNVRQVAVFDTAFHSHLPEVAAIYPGPYDWQQQGIRR
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHH
YGFHGISHQYCTQRTAEILGRGVDRLIICHLGNGASLTAVKQGQSIDTSMGFTPLEGLMM
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHE
GTRSGSIDPGILIHLMRQGYDADQLDHMLNKASGLKGISGVSHDLREIEQAIAQDNAQAK
ECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHH
LARDLYLHRLKACLGSMLMSLGGVDVLVFTGGIGEHSASVRAETCEALAFLGLKLDPILN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHCC
RKSPVDQDIAAVDSKVRVLVIKTQEDWAIAQSCYEQCC
CCCCCCHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA