Click here to switch to the map view.

The map label for this gene is pyk1 [H]

Identifier: 158333642

GI number: 158333642

Start: 424534

End: 426492

Strand: Direct

Name: pyk1 [H]

Synonym: AM1_0444

Alternate gene names: 158333642

Gene position: 424534-426492 (Clockwise)

Preceding gene: 158333641

Following gene: 158333643

Centisome position: 6.53

GC content: 54.06

Gene sequence:

>1959_bases
ATGCCAAGGAACGGATGCGCAACAAGATCATCGAATGTCTCGCCTATGCCCATGAGCATGGTAAGGATCAAGATGAGATT
GTCAACTGGACCTGGCCCTATGTTGGAGTCTGTGAATAATATCGATGCTCCGCGCATTGAGAGTGCCATTCGGGCGCTGG
AGCAGCTCAAAGCGCGCGCCTTGGCCCTTGAGCAGGATTTCGAAATTGCTCTCCAGCGCACCGCTCCCTCTCATCGCAAT
AGTGCTCGTAATCTCCTCCACTATCTGGCACTGCGCCGCCATGATATCCGCGAGCTGCAGCTTGAACTCAGCCACCTGGG
CCTGAGTTCCCTGGGGTTAACCGAACTGCACGTGATGGCCTCTCTCAATGCCGTCCTGATTGCCTTGCATCGACTAGTAG
AACGAGCGGTGCCTCCTCAACTTCAAGCCCCGACCCCTGTGACCTTTGCCAATGGTCAGGCTCTCCTGGTGGAACATACC
CTCACAGCCCTAGGGCCTAAACCAGAACAGCGAGCCACTCGGATCATGGTGACGATGCCCACTACAGCAGCAGAGGATCC
AGCTCTGATTCGTCGGTTTTTGGCTGCTGGGATGGATATTATGCGGATTAACTGCGCCCATGATGGTCCTGATGCTTGGC
TGCAAATGATTCAGCATCTGCGACAAGCGGAAGCGGAATTGGGACTATCCTGCAAAGTCTCTTTTGATTTGGCCGGTCCC
AAACTGAGAACCGAGGCTTTGGCCCCTGATGTTGAAGTGATCAAATGGCAGCCCCACCGAGATCGTCTGGGGCACATCGT
GCAACCGGCACGGCTCTGGTTGACCTCAACGTCCTCCCAGACTGCTGACTTAGATTGCACTACCATCCCGATTCAGTCTC
CCATCTCAGTCCAAATGGGAGATATGATCCAGCTTACGGATGCCCGTCACCGTCGCCGCCGCTTATCAGTGGTGGAGGTG
AGGGATAATGGCTGTCTCTGTGAATGCGATCGCACCGGCTATATCACTTCAGGACTGCCCTTTGAGATTCAGCGTCATCA
GGCCGTAGTAGGAACGGGCAAGATCGGTCAGCTTCCTGCCCTTGAAAACAACCTCACCCTCCAACCTGGAGATGTCCTGC
GCATTCTTCGGGGTGATATTCTAGGCCAGCCCGCCATCTTAGATGCCACCGGACAGGTTCTGCAGCCTGCCCACGTGGGC
TGCACCTTGCCCCAAGTGTTCCAGGATGTGAAGCCAGGAGAGCGGATCTTTTTTGACGATGGCAAGATTGAGGGGCTGAT
TCAAAAGGTTAGTGAGACTGAACTGAGGGTGGAAATTGTTTCTGCAGTCAAAGGAATCGCCAAGCTCAAGGGAGAAAAGG
GTATCAACCTGCCTGACACCCAACTTAACCTGCCAGCCCTCACCCCCAAGGATATTAGCGATCTCAGCTTTGTGGCCCAA
CAGGGAGACTTAGTGGCCCTCTCCTTTGTCCAATCCCCAGAGGATGTAGAACATCTGGTGCAAAAGCTCGATCAATTGGG
GGCAACAGACTTAGGCATTATTCTCAAAATTGAAACCCGACTCGGGTTTGAGAATTTACCCAAACTATTGCTGGCTGCAA
TGCAGCATCGATCTGTAGCTGTGATGATTGCGCGGGGCGATTTGGGGGTTGAGGTGGGCTTTGAGCGGATGTCGGAGGTG
CAGGAGCAAATTTTGCTGTTGTGCCAAGCGGCCCATGTTCCCGTTATCTGGGCAACTCAGGTCCTAGAGTCCCTGGCGAA
GGGAGGCCTACCGTCACGGGCGGAGGTTACAGATGCAGCGATGGGCAGTCGAGCCGAATGCGTCATGCTCAACAAAGGCC
CCTATATTGAGCGGGCGATGGAATTTCTCAACAATATTCTTCAGCGGATGCAGGAAAATGTGGCTAAGAATATGGCCACC
CTTCGCAAACTCCGGGTTTCCGAGATTGAAGCGGAGTAG

Upstream 100 bases:

>100_bases
AATACGCCCCTGGAGTTAGCGATTAAAAATGAGGTGGATCGCTTTAACCTGGTGATTGATGTGATTGATCGGGTGCCAAA
GCTGGGGTCGGCAGCGGGCC

Downstream 100 bases:

>100_bases
TCTCGTCCTCAAGCCCTGTTTGCTCGTTACCAGCCATCTGTGGGCCAGATCATGAAGATTTTAGTTCTCAATGCCGGTTC
TAGCAGCCACAAATGTTGCC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MPRNGCATRSSNVSPMPMSMVRIKMRLSTGPGPMLESVNNIDAPRIESAIRALEQLKARALALEQDFEIALQRTAPSHRN
SARNLLHYLALRRHDIRELQLELSHLGLSSLGLTELHVMASLNAVLIALHRLVERAVPPQLQAPTPVTFANGQALLVEHT
LTALGPKPEQRATRIMVTMPTTAAEDPALIRRFLAAGMDIMRINCAHDGPDAWLQMIQHLRQAEAELGLSCKVSFDLAGP
KLRTEALAPDVEVIKWQPHRDRLGHIVQPARLWLTSTSSQTADLDCTTIPIQSPISVQMGDMIQLTDARHRRRRLSVVEV
RDNGCLCECDRTGYITSGLPFEIQRHQAVVGTGKIGQLPALENNLTLQPGDVLRILRGDILGQPAILDATGQVLQPAHVG
CTLPQVFQDVKPGERIFFDDGKIEGLIQKVSETELRVEIVSAVKGIAKLKGEKGINLPDTQLNLPALTPKDISDLSFVAQ
QGDLVALSFVQSPEDVEHLVQKLDQLGATDLGIILKIETRLGFENLPKLLLAAMQHRSVAVMIARGDLGVEVGFERMSEV
QEQILLLCQAAHVPVIWATQVLESLAKGGLPSRAEVTDAAMGSRAECVMLNKGPYIERAMEFLNNILQRMQENVAKNMAT
LRKLRVSEIEAE

Sequences:

>Translated_652_residues
MPRNGCATRSSNVSPMPMSMVRIKMRLSTGPGPMLESVNNIDAPRIESAIRALEQLKARALALEQDFEIALQRTAPSHRN
SARNLLHYLALRRHDIRELQLELSHLGLSSLGLTELHVMASLNAVLIALHRLVERAVPPQLQAPTPVTFANGQALLVEHT
LTALGPKPEQRATRIMVTMPTTAAEDPALIRRFLAAGMDIMRINCAHDGPDAWLQMIQHLRQAEAELGLSCKVSFDLAGP
KLRTEALAPDVEVIKWQPHRDRLGHIVQPARLWLTSTSSQTADLDCTTIPIQSPISVQMGDMIQLTDARHRRRRLSVVEV
RDNGCLCECDRTGYITSGLPFEIQRHQAVVGTGKIGQLPALENNLTLQPGDVLRILRGDILGQPAILDATGQVLQPAHVG
CTLPQVFQDVKPGERIFFDDGKIEGLIQKVSETELRVEIVSAVKGIAKLKGEKGINLPDTQLNLPALTPKDISDLSFVAQ
QGDLVALSFVQSPEDVEHLVQKLDQLGATDLGIILKIETRLGFENLPKLLLAAMQHRSVAVMIARGDLGVEVGFERMSEV
QEQILLLCQAAHVPVIWATQVLESLAKGGLPSRAEVTDAAMGSRAECVMLNKGPYIERAMEFLNNILQRMQENVAKNMAT
LRKLRVSEIEAE
>Mature_651_residues
PRNGCATRSSNVSPMPMSMVRIKMRLSTGPGPMLESVNNIDAPRIESAIRALEQLKARALALEQDFEIALQRTAPSHRNS
ARNLLHYLALRRHDIRELQLELSHLGLSSLGLTELHVMASLNAVLIALHRLVERAVPPQLQAPTPVTFANGQALLVEHTL
TALGPKPEQRATRIMVTMPTTAAEDPALIRRFLAAGMDIMRINCAHDGPDAWLQMIQHLRQAEAELGLSCKVSFDLAGPK
LRTEALAPDVEVIKWQPHRDRLGHIVQPARLWLTSTSSQTADLDCTTIPIQSPISVQMGDMIQLTDARHRRRRLSVVEVR
DNGCLCECDRTGYITSGLPFEIQRHQAVVGTGKIGQLPALENNLTLQPGDVLRILRGDILGQPAILDATGQVLQPAHVGC
TLPQVFQDVKPGERIFFDDGKIEGLIQKVSETELRVEIVSAVKGIAKLKGEKGINLPDTQLNLPALTPKDISDLSFVAQQ
GDLVALSFVQSPEDVEHLVQKLDQLGATDLGIILKIETRLGFENLPKLLLAAMQHRSVAVMIARGDLGVEVGFERMSEVQ
EQILLLCQAAHVPVIWATQVLESLAKGGLPSRAEVTDAAMGSRAECVMLNKGPYIERAMEFLNNILQRMQENVAKNMATL
RKLRVSEIEAE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=243, Percent_Identity=34.9794238683128, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI33286422, Length=213, Percent_Identity=37.5586854460094, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33286420, Length=213, Percent_Identity=37.5586854460094, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI10835121, Length=216, Percent_Identity=35.6481481481481, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI32967597, Length=216, Percent_Identity=35.6481481481481, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI310128732, Length=171, Percent_Identity=36.2573099415205, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI310128730, Length=171, Percent_Identity=36.2573099415205, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1787965, Length=261, Percent_Identity=33.7164750957854, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788160, Length=206, Percent_Identity=31.5533980582524, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17544584, Length=236, Percent_Identity=36.4406779661017, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71984413, Length=230, Percent_Identity=34.3478260869565, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17506831, Length=230, Percent_Identity=34.3478260869565, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17506829, Length=230, Percent_Identity=34.3478260869565, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71984406, Length=230, Percent_Identity=34.3478260869565, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324923, Length=222, Percent_Identity=36.4864864864865, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319279, Length=214, Percent_Identity=36.4485981308411, Blast_Score=113, Evalue=9e-26,
Organism=Drosophila melanogaster, GI28571814, Length=213, Percent_Identity=37.5586854460094, Blast_Score=127, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648964, Length=213, Percent_Identity=37.5586854460094, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648966, Length=220, Percent_Identity=38.1818181818182, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24581235, Length=255, Percent_Identity=27.4509803921569, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24646914, Length=148, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 71671; Mature: 71540

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNGCATRSSNVSPMPMSMVRIKMRLSTGPGPMLESVNNIDAPRIESAIRALEQLKARA
CCCCCCCCCCCCCCCCCHHHEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LALEQDFEIALQRTAPSHRNSARNLLHYLALRRHDIRELQLELSHLGLSSLGLTELHVMA
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SLNAVLIALHRLVERAVPPQLQAPTPVTFANGQALLVEHTLTALGPKPEQRATRIMVTMP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEHHHHHCCCCCCHHHCEEEEEEC
TTAAEDPALIRRFLAAGMDIMRINCAHDGPDAWLQMIQHLRQAEAELGLSCKVSFDLAGP
CCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
KLRTEALAPDVEVIKWQPHRDRLGHIVQPARLWLTSTSSQTADLDCTTIPIQSPISVQMG
CHHHHHCCCCCEEEEECCCHHHHHHHCCCHHEEEECCCCCCCCCCEEEECCCCCCCCCCC
DMIQLTDARHRRRRLSVVEVRDNGCLCECDRTGYITSGLPFEIQRHQAVVGTGKIGQLPA
CEEEEHHHHHHHHCCEEEEEECCCCEEEECCCCEECCCCCCEEECCEEEEECCCCCCCCC
LENNLTLQPGDVLRILRGDILGQPAILDATGQVLQPAHVGCTLPQVFQDVKPGERIFFDD
CCCCCEECCHHHHHHHHHHHCCCCCEECCCCCCCCCHHCCCCHHHHHHHCCCCCEEEECC
GKIEGLIQKVSETELRVEIVSAVKGIAKLKGEKGINLPDTQLNLPALTPKDISDLSFVAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
QGDLVALSFVQSPEDVEHLVQKLDQLGATDLGIILKIETRLGFENLPKLLLAAMQHRSVA
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
VMIARGDLGVEVGFERMSEVQEQILLLCQAAHVPVIWATQVLESLAKGGLPSRAEVTDAA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
MGSRAECVMLNKGPYIERAMEFLNNILQRMQENVAKNMATLRKLRVSEIEAE
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRNGCATRSSNVSPMPMSMVRIKMRLSTGPGPMLESVNNIDAPRIESAIRALEQLKARA
CCCCCCCCCCCCCCCCHHHEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LALEQDFEIALQRTAPSHRNSARNLLHYLALRRHDIRELQLELSHLGLSSLGLTELHVMA
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
SLNAVLIALHRLVERAVPPQLQAPTPVTFANGQALLVEHTLTALGPKPEQRATRIMVTMP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEHHHHHCCCCCCHHHCEEEEEEC
TTAAEDPALIRRFLAAGMDIMRINCAHDGPDAWLQMIQHLRQAEAELGLSCKVSFDLAGP
CCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
KLRTEALAPDVEVIKWQPHRDRLGHIVQPARLWLTSTSSQTADLDCTTIPIQSPISVQMG
CHHHHHCCCCCEEEEECCCHHHHHHHCCCHHEEEECCCCCCCCCCEEEECCCCCCCCCCC
DMIQLTDARHRRRRLSVVEVRDNGCLCECDRTGYITSGLPFEIQRHQAVVGTGKIGQLPA
CEEEEHHHHHHHHCCEEEEEECCCCEEEECCCCEECCCCCCEEECCEEEEECCCCCCCCC
LENNLTLQPGDVLRILRGDILGQPAILDATGQVLQPAHVGCTLPQVFQDVKPGERIFFDD
CCCCCEECCHHHHHHHHHHHCCCCCEECCCCCCCCCHHCCCCHHHHHHHCCCCCEEEECC
GKIEGLIQKVSETELRVEIVSAVKGIAKLKGEKGINLPDTQLNLPALTPKDISDLSFVAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
QGDLVALSFVQSPEDVEHLVQKLDQLGATDLGIILKIETRLGFENLPKLLLAAMQHRSVA
CCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
VMIARGDLGVEVGFERMSEVQEQILLLCQAAHVPVIWATQVLESLAKGGLPSRAEVTDAA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
MGSRAECVMLNKGPYIERAMEFLNNILQRMQENVAKNMATLRKLRVSEIEAE
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]