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The map label for this gene is kinE [H]

Identifier: 158333594

GI number: 158333594

Start: 367810

End: 369990

Strand: Reverse

Name: kinE [H]

Synonym: AM1_0395

Alternate gene names: 158333594

Gene position: 369990-367810 (Counterclockwise)

Preceding gene: 158333595

Following gene: 158333590

Centisome position: 5.69

GC content: 48.05

Gene sequence:

>2181_bases
GTGCCCTTGCGGTTTTATCCTTTTACCCAACTTTTTCAAGCCCGACTCTCGCGCCGAATCGAGCTATGGGTATTTCTGAG
CATTATTGCAATTGAAGCCGTGATTCTTATCCCTTCCGTTTACCGACGTGAAGCTGAGCTACTTAAGCACCTGACAGAGA
TCTCAGCTGCTAAAGCCACAGGGTTACTGGCAAACGAGGAAAATGAAATCACCGATCAAACGGTCCTAGACAAACTTGTA
GTGATCGAGGAAAATCCAGCCGTCACCGGTGGGATTCTATTTCGGACTGATGGCACCAAAGTTGGGGAGTTTGGTGAACA
ACCTGAGCTTACCTTTGAGCAGGCTACTCGGCGTTCCGAAGAAACATATCTCAATCGAGCCCTACAACGATACGATGCCA
CCTGGCCCATGCCCTTGCTCGATAAATATGTGCTAATTGTTCGCCATGATGCTAGCAATGTCCAAAACGAACTCAATCAG
TTTATTTGGCGGATCATTGGCCTGGTGGTCATTATTTCCTTGTTTGTGTCCTTAGCAACCATGATTGTTTTGCAGTCCTT
TGTGATTGCGCCCATTTTGCAGCTGCGGAAAGATTTGCTTCAGGCGGGGACCTTCTTAGCGACACCACCCGGCAGTTCAC
CGCCAGAGTTTGAGTCCACCCAGAATCCACCGGATGATGAATTGGGGGATGTGATTCAAACCTTCGAGCAGATGTTTAAT
CAGATTCGCGATGCGATCGCATCCCGGCAGCAGGCTGAATCTGACCTCAGACTATCAGAAGAGAAATTTTCCAAAGCATT
CCGAGCTAGCCCCAACCCCATCACCATGAGTACTCTTGAGGATGGTTGTCTGGTTGAAGTGAATGACAGCTTTTTGGATT
TATTTGGGTTGACCGCTTCAGAGGTATTAGGTCGGACCAGTTTAGAACTGGATCTATGGGCCGATCCAGCGGATCGAATG
CAGATGATTCAGGCCATTCTCAAGGATGGCATTATTCGAAATTATGAGTATCGATTGTGCGATCGCAACGGCAAAGAAAA
GACGGTTCTCTATTCTTCCGAACAGCTGAAGGTCAATGACCAGGACTGCATCCTCTCCGTCATTAATGACATTACCGAAC
GCAAAGAGGCTGAAATTGCCCTAGAGGCCAGCGAAGAGCGATTTCGGATGTTGGTGGACCAAGCGGTCGATGCCATTTAT
GTGGTTAATGAGCAGGGGCAGTTTGTGGATGTGAACCAGCAAGCCTGTGCCAACTTGGGGTATACCCGGGATGAATTATT
AGGGTGTCGTGTACCAGATATTCAAAAAAGTTTGTCAGACGACGATTTTGTGAATTTATGGCAACGACTGCTGCCGAAAG
ATCCTCTCACCTTTATTGGCACTCATCAGCGCAAAGACGGCAGTCAGTTTCCCGTCGAAGTTCGGTTAGGGTTATTTGAA
GCGGGAGAAGAACAGTTACTCCTAGCCCTAGCGCGAGATATTACAGAGCGACGACAAGCGGAAAAGGATCGAGAGCGACT
GGCGGAAATTGGGGAACTGGCCGCCATGATTGTCCATGAAGTCCGTAATCCTCTAACAACAGTCTTGATGGGATTGAATT
CGTTCCAGCGCATGGACTTACCCGACCGGGCTAAACTACGGTTGCAGTTGGCCTTAGAGGAGTCAGAACGGTTGCAACGA
CTCCTGAATGAAATTTTGCTCTATGCCAAACAGCAAAAGTTAGACACAACAGCCGTTGAGCTGAATCAGTTGTTTGCCGA
TATGCTGCCGACCTTACAAGAAATGCCCAGTGCAGCAGATCGCCCGATTCAGCTGATGCCTGCTGATGAGTCGGCATGGG
TAGACGCTGATATCGATAAACTGAAGCAAGTCTTTATCAATTTAGTTAGCAATGCCTGTGAAGCGGTTCCCGAAGGTGAA
CCCATTATTTGGCAGCTTCAGATTGTAGCGCTACAAGATCAAGTCAAGATTGAAGTCAATAATGGGGGAGATCCGATTCC
CGCAGATGTGCTTCCCCAATTAACGAAGCCGTTTTTTACCACCAAGTCATCTGGAAATGGGTTGGGACTGGCAATTACCA
AGCGGATTGTGGAGACCCATGGTGGCGAATTAGTAATCCAATCTTCAGCTGCTAGTGGCACTACCGTTACCGTGTCTTTA
CCAACAGTTAATTCTCCCTAG

Upstream 100 bases:

>100_bases
GAGTTTGGATTGGAGCGATTCTTGCAGGTTAATGCCCGATACTCATAAAACCCATCCCCCTTACAGGGATAATGGGGATC
AGTGTTAGCTGAGCGTTGTA

Downstream 100 bases:

>100_bases
GAATTTAAGCGGCAGCGCTGGGAAGCTGAGCAGTCAGGGTCTCCATCAAATCGCTAATACTGTAGGGTTTGACAACATAG
GCATTGGCACCTGCGGCCAT

Product: sensory transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MPLRFYPFTQLFQARLSRRIELWVFLSIIAIEAVILIPSVYRREAELLKHLTEISAAKATGLLANEENEITDQTVLDKLV
VIEENPAVTGGILFRTDGTKVGEFGEQPELTFEQATRRSEETYLNRALQRYDATWPMPLLDKYVLIVRHDASNVQNELNQ
FIWRIIGLVVIISLFVSLATMIVLQSFVIAPILQLRKDLLQAGTFLATPPGSSPPEFESTQNPPDDELGDVIQTFEQMFN
QIRDAIASRQQAESDLRLSEEKFSKAFRASPNPITMSTLEDGCLVEVNDSFLDLFGLTASEVLGRTSLELDLWADPADRM
QMIQAILKDGIIRNYEYRLCDRNGKEKTVLYSSEQLKVNDQDCILSVINDITERKEAEIALEASEERFRMLVDQAVDAIY
VVNEQGQFVDVNQQACANLGYTRDELLGCRVPDIQKSLSDDDFVNLWQRLLPKDPLTFIGTHQRKDGSQFPVEVRLGLFE
AGEEQLLLALARDITERRQAEKDRERLAEIGELAAMIVHEVRNPLTTVLMGLNSFQRMDLPDRAKLRLQLALEESERLQR
LLNEILLYAKQQKLDTTAVELNQLFADMLPTLQEMPSAADRPIQLMPADESAWVDADIDKLKQVFINLVSNACEAVPEGE
PIIWQLQIVALQDQVKIEVNNGGDPIPADVLPQLTKPFFTTKSSGNGLGLAITKRIVETHGGELVIQSSAASGTTVTVSL
PTVNSP

Sequences:

>Translated_726_residues
MPLRFYPFTQLFQARLSRRIELWVFLSIIAIEAVILIPSVYRREAELLKHLTEISAAKATGLLANEENEITDQTVLDKLV
VIEENPAVTGGILFRTDGTKVGEFGEQPELTFEQATRRSEETYLNRALQRYDATWPMPLLDKYVLIVRHDASNVQNELNQ
FIWRIIGLVVIISLFVSLATMIVLQSFVIAPILQLRKDLLQAGTFLATPPGSSPPEFESTQNPPDDELGDVIQTFEQMFN
QIRDAIASRQQAESDLRLSEEKFSKAFRASPNPITMSTLEDGCLVEVNDSFLDLFGLTASEVLGRTSLELDLWADPADRM
QMIQAILKDGIIRNYEYRLCDRNGKEKTVLYSSEQLKVNDQDCILSVINDITERKEAEIALEASEERFRMLVDQAVDAIY
VVNEQGQFVDVNQQACANLGYTRDELLGCRVPDIQKSLSDDDFVNLWQRLLPKDPLTFIGTHQRKDGSQFPVEVRLGLFE
AGEEQLLLALARDITERRQAEKDRERLAEIGELAAMIVHEVRNPLTTVLMGLNSFQRMDLPDRAKLRLQLALEESERLQR
LLNEILLYAKQQKLDTTAVELNQLFADMLPTLQEMPSAADRPIQLMPADESAWVDADIDKLKQVFINLVSNACEAVPEGE
PIIWQLQIVALQDQVKIEVNNGGDPIPADVLPQLTKPFFTTKSSGNGLGLAITKRIVETHGGELVIQSSAASGTTVTVSL
PTVNSP
>Mature_725_residues
PLRFYPFTQLFQARLSRRIELWVFLSIIAIEAVILIPSVYRREAELLKHLTEISAAKATGLLANEENEITDQTVLDKLVV
IEENPAVTGGILFRTDGTKVGEFGEQPELTFEQATRRSEETYLNRALQRYDATWPMPLLDKYVLIVRHDASNVQNELNQF
IWRIIGLVVIISLFVSLATMIVLQSFVIAPILQLRKDLLQAGTFLATPPGSSPPEFESTQNPPDDELGDVIQTFEQMFNQ
IRDAIASRQQAESDLRLSEEKFSKAFRASPNPITMSTLEDGCLVEVNDSFLDLFGLTASEVLGRTSLELDLWADPADRMQ
MIQAILKDGIIRNYEYRLCDRNGKEKTVLYSSEQLKVNDQDCILSVINDITERKEAEIALEASEERFRMLVDQAVDAIYV
VNEQGQFVDVNQQACANLGYTRDELLGCRVPDIQKSLSDDDFVNLWQRLLPKDPLTFIGTHQRKDGSQFPVEVRLGLFEA
GEEQLLLALARDITERRQAEKDRERLAEIGELAAMIVHEVRNPLTTVLMGLNSFQRMDLPDRAKLRLQLALEESERLQRL
LNEILLYAKQQKLDTTAVELNQLFADMLPTLQEMPSAADRPIQLMPADESAWVDADIDKLKQVFINLVSNACEAVPEGEP
IIWQLQIVALQDQVKIEVNNGGDPIPADVLPQLTKPFFTTKSSGNGLGLAITKRIVETHGGELVIQSSAASGTTVTVSLP
TVNSP

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=34.6846846846847, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788549, Length=347, Percent_Identity=28.8184438040346, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI48994928, Length=377, Percent_Identity=23.6074270557029, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1786600, Length=351, Percent_Identity=25.6410256410256, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786783, Length=277, Percent_Identity=28.8808664259928, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=26.0536398467433, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081816, Length=239, Percent_Identity=29.2887029288703, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788713, Length=266, Percent_Identity=25.9398496240602, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790551, Length=236, Percent_Identity=28.3898305084746, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790300, Length=249, Percent_Identity=27.3092369477912, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81577; Mature: 81446

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRFYPFTQLFQARLSRRIELWVFLSIIAIEAVILIPSVYRREAELLKHLTEISAAKAT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLANEENEITDQTVLDKLVVIEENPAVTGGILFRTDGTKVGEFGEQPELTFEQATRRSE
CCCCCCCCCHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
ETYLNRALQRYDATWPMPLLDKYVLIVRHDASNVQNELNQFIWRIIGLVVIISLFVSLAT
HHHHHHHHHHHCCCCCCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIVLQSFVIAPILQLRKDLLQAGTFLATPPGSSPPEFESTQNPPDDELGDVIQTFEQMFN
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QIRDAIASRQQAESDLRLSEEKFSKAFRASPNPITMSTLEDGCLVEVNDSFLDLFGLTAS
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHHHCCCHH
EVLGRTSLELDLWADPADRMQMIQAILKDGIIRNYEYRLCDRNGKEKTVLYSSEQLKVND
HHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCEEECC
QDCILSVINDITERKEAEIALEASEERFRMLVDQAVDAIYVVNEQGQFVDVNQQACANLG
HHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHCC
YTRDELLGCRVPDIQKSLSDDDFVNLWQRLLPKDPLTFIGTHQRKDGSQFPVEVRLGLFE
CCHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECHHH
AGEEQLLLALARDITERRQAEKDRERLAEIGELAAMIVHEVRNPLTTVLMGLNSFQRMDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDRAKLRLQLALEESERLQRLLNEILLYAKQQKLDTTAVELNQLFADMLPTLQEMPSAAD
CCHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPIQLMPADESAWVDADIDKLKQVFINLVSNACEAVPEGEPIIWQLQIVALQDQVKIEVN
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCEEEEEEC
NGGDPIPADVLPQLTKPFFTTKSSGNGLGLAITKRIVETHGGELVIQSSAASGTTVTVSL
CCCCCCCHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEE
PTVNSP
CCCCCC
>Mature Secondary Structure 
PLRFYPFTQLFQARLSRRIELWVFLSIIAIEAVILIPSVYRREAELLKHLTEISAAKAT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLANEENEITDQTVLDKLVVIEENPAVTGGILFRTDGTKVGEFGEQPELTFEQATRRSE
CCCCCCCCCHHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
ETYLNRALQRYDATWPMPLLDKYVLIVRHDASNVQNELNQFIWRIIGLVVIISLFVSLAT
HHHHHHHHHHHCCCCCCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MIVLQSFVIAPILQLRKDLLQAGTFLATPPGSSPPEFESTQNPPDDELGDVIQTFEQMFN
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QIRDAIASRQQAESDLRLSEEKFSKAFRASPNPITMSTLEDGCLVEVNDSFLDLFGLTAS
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCEEEECHHHHHHHCCCHH
EVLGRTSLELDLWADPADRMQMIQAILKDGIIRNYEYRLCDRNGKEKTVLYSSEQLKVND
HHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCEEECC
QDCILSVINDITERKEAEIALEASEERFRMLVDQAVDAIYVVNEQGQFVDVNQQACANLG
HHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHCCEEEEECCCCCEEECCHHHHHHCC
YTRDELLGCRVPDIQKSLSDDDFVNLWQRLLPKDPLTFIGTHQRKDGSQFPVEVRLGLFE
CCHHHHCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECHHH
AGEEQLLLALARDITERRQAEKDRERLAEIGELAAMIVHEVRNPLTTVLMGLNSFQRMDL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDRAKLRLQLALEESERLQRLLNEILLYAKQQKLDTTAVELNQLFADMLPTLQEMPSAAD
CCHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RPIQLMPADESAWVDADIDKLKQVFINLVSNACEAVPEGEPIIWQLQIVALQDQVKIEVN
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCEEEEEEC
NGGDPIPADVLPQLTKPFFTTKSSGNGLGLAITKRIVETHGGELVIQSSAASGTTVTVSL
CCCCCCCHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEE
PTVNSP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]