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The map label for this gene is sphX [H]

Identifier: 158333396

GI number: 158333396

Start: 168971

End: 169963

Strand: Reverse

Name: sphX [H]

Synonym: AM1_0168

Alternate gene names: 158333396

Gene position: 169963-168971 (Counterclockwise)

Preceding gene: 158333397

Following gene: 158333394

Centisome position: 2.61

GC content: 46.02

Gene sequence:

>993_bases
ATGCAGCTGAATCGTTCGACCCTGACAGGTGCGGTTGTTTCGCTAGCGGTCGCCGTCAGCACAACTGCTTTCTCGGCCAC
TTCTGCCGATGCCCAAACTAAAAAGATAAAAATTGACGGTTCCAGTACCGTATTTCCAATCAGTGAAGCCGTTGCTGAGG
AATTTCAAAAGTTAACAAATAACAAGGTTAACGTCACAGTCGGTGTGTCTGGAACCGGTGGCGGTTTTAAGAAGTTTTGT
GCAGGTGAAACAGATATTTCTGACGCATCTCGACCCATTAAAGAGAAAGAACAAAAGCTTTGTGAAGGGGTTGAGTATAT
CGAAATCCCTGTTGCTTTTGATGCTTTAACCGTTGTGGTCAACAAAGGCAATCCCATCAAGAGTATGACCGTTGCTGAAC
TCAAGAAGATGTGGTCAGAAAACCCAGAGGGTTCTGTTACTACATGGAAAGAAGTCAATTCCAGCTGGCCCGCTGATAAA
ATCAGCTTTTATGCCCCAGGTTCTGATTCGGGTACTTTTGACTACTTCAATGGCACCATTGCCAAGAAAAATCATCGCAA
AGATATAACCGCCAGCGAAGATGACAATATTTTGGTTCAAGGCGTTTCCCGTGAGCCCAATGGAATTGGATATTTTGGAT
TTGCCTACTATGAGCAAAATAAAGATCGCCTGAATGCAGTGGCGATTGACAATGGTAATGGACCCGTCCTCCCCTCCCGT
GAAGCAGTGGAGAATGGCACCTACACGCCTCTGTCTCGCCCTATCTTTATTTATGTCAATAAGAAGGCCGCCAAGCGTCC
TGAAGTTCGCCAGTTTGTGAACTACTACCTCAGAAAAGCTGCTGAGTTTTCTGCTGAAGTCGGCTATATCCCTTTACCTG
CTCCGGCTTATCAGCAAGCGGCTAAAAATTTCCGCGAAGGCAAGACTGGCAGCTTCTTTGCGGGTAGAGATACAGATGGC
CTAAAGGTCTCTGAAGTGCTCAAATCCAAATAG

Upstream 100 bases:

>100_bases
ATTTTCCATTAACTGACCTTAATCTTGCTTAGGTATTCTTGCTTTGGCTTTAAGTCATTTTTTCTAAATTACGTATCGAT
TGTTAATTATGGAGAAAGGT

Downstream 100 bases:

>100_bases
ACAACAAGCTAACTTTGGTTTTCTCACACTCTGCTGATTGTTCCTGAGGAAGTTCACGAGTTCTTCCTCAGGAACTTCTT
TTTTGAAAAGTTGATTTTAA

Product: phosphate ABC transporter periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MQLNRSTLTGAVVSLAVAVSTTAFSATSADAQTKKIKIDGSSTVFPISEAVAEEFQKLTNNKVNVTVGVSGTGGGFKKFC
AGETDISDASRPIKEKEQKLCEGVEYIEIPVAFDALTVVVNKGNPIKSMTVAELKKMWSENPEGSVTTWKEVNSSWPADK
ISFYAPGSDSGTFDYFNGTIAKKNHRKDITASEDDNILVQGVSREPNGIGYFGFAYYEQNKDRLNAVAIDNGNGPVLPSR
EAVENGTYTPLSRPIFIYVNKKAAKRPEVRQFVNYYLRKAAEFSAEVGYIPLPAPAYQQAAKNFREGKTGSFFAGRDTDG
LKVSEVLKSK

Sequences:

>Translated_330_residues
MQLNRSTLTGAVVSLAVAVSTTAFSATSADAQTKKIKIDGSSTVFPISEAVAEEFQKLTNNKVNVTVGVSGTGGGFKKFC
AGETDISDASRPIKEKEQKLCEGVEYIEIPVAFDALTVVVNKGNPIKSMTVAELKKMWSENPEGSVTTWKEVNSSWPADK
ISFYAPGSDSGTFDYFNGTIAKKNHRKDITASEDDNILVQGVSREPNGIGYFGFAYYEQNKDRLNAVAIDNGNGPVLPSR
EAVENGTYTPLSRPIFIYVNKKAAKRPEVRQFVNYYLRKAAEFSAEVGYIPLPAPAYQQAAKNFREGKTGSFFAGRDTDG
LKVSEVLKSK
>Mature_330_residues
MQLNRSTLTGAVVSLAVAVSTTAFSATSADAQTKKIKIDGSSTVFPISEAVAEEFQKLTNNKVNVTVGVSGTGGGFKKFC
AGETDISDASRPIKEKEQKLCEGVEYIEIPVAFDALTVVVNKGNPIKSMTVAELKKMWSENPEGSVTTWKEVNSSWPADK
ISFYAPGSDSGTFDYFNGTIAKKNHRKDITASEDDNILVQGVSREPNGIGYFGFAYYEQNKDRLNAVAIDNGNGPVLPSR
EAVENGTYTPLSRPIFIYVNKKAAKRPEVRQFVNYYLRKAAEFSAEVGYIPLPAPAYQQAAKNFREGKTGSFFAGRDTDG
LKVSEVLKSK

Specific function: May be involved in the system for phosphate transport across the cytoplasmic membrane [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:
GO:0006810: Phosphate ABC transporter, periplasmic phosphate-binding protein

Cell location: Cell inner membrane. Note=Found in the cytoplasmic membrane in a form that is closely associated with the cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35880; Mature: 35880

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNRSTLTGAVVSLAVAVSTTAFSATSADAQTKKIKIDGSSTVFPISEAVAEEFQKLTN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHC
NKVNVTVGVSGTGGGFKKFCAGETDISDASRPIKEKEQKLCEGVEYIEIPVAFDALTVVV
CCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEEEEE
NKGNPIKSMTVAELKKMWSENPEGSVTTWKEVNSSWPADKISFYAPGSDSGTFDYFNGTI
ECCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCCEEEECCCCCCCCEEECCCEE
AKKNHRKDITASEDDNILVQGVSREPNGIGYFGFAYYEQNKDRLNAVAIDNGNGPVLPSR
ECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCH
EAVENGTYTPLSRPIFIYVNKKAAKRPEVRQFVNYYLRKAAEFSAEVGYIPLPAPAYQQA
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHH
AKNFREGKTGSFFAGRDTDGLKVSEVLKSK
HHHHHCCCCCCEECCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MQLNRSTLTGAVVSLAVAVSTTAFSATSADAQTKKIKIDGSSTVFPISEAVAEEFQKLTN
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHC
NKVNVTVGVSGTGGGFKKFCAGETDISDASRPIKEKEQKLCEGVEYIEIPVAFDALTVVV
CCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEEEEEEEE
NKGNPIKSMTVAELKKMWSENPEGSVTTWKEVNSSWPADKISFYAPGSDSGTFDYFNGTI
ECCCCCCHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCCEEEECCCCCCCCEEECCCEE
AKKNHRKDITASEDDNILVQGVSREPNGIGYFGFAYYEQNKDRLNAVAIDNGNGPVLPSR
ECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCH
EAVENGTYTPLSRPIFIYVNKKAAKRPEVRQFVNYYLRKAAEFSAEVGYIPLPAPAYQQA
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHH
AKNFREGKTGSFFAGRDTDGLKVSEVLKSK
HHHHHCCCCCCEECCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7741855 [H]