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The map label for this gene is ispG

Identifier: 158333377

GI number: 158333377

Start: 148029

End: 149255

Strand: Direct

Name: ispG

Synonym: AM1_0149

Alternate gene names: 158333377

Gene position: 148029-149255 (Clockwise)

Preceding gene: 158333376

Following gene: 158333379

Centisome position: 2.28

GC content: 50.77

Gene sequence:

>1227_bases
ATGCAGACTTTATCAACCCCTGTCACAGGAACAGACGAGCGGTCCACCCCTGTTTCTACATCAATCACTCGGCGTAAGAC
TCGCCCCGTTCCCGTTGGGGATGTGATTATTGGTGGAGGCCATCCTGTGGTGGTTCAGTCCATGATCAATGAAGATACCC
TCGACATTGAGGGCTCTGTAGCTGCGATTCGTCGTCTGCATGAGTTGGGTTGCGAAATCGTGCGGGTGACCGTACCCAGC
ATGGCCCATGCTAAAGCCCTCGCGACCATCAAAGAAAAATTAACAGCCACCTACCAGACGGTCCCCCTGGTAGCAGATGT
TCACCACAACGGCATGAAAATTGCCCTGGAAGTGGCGAAGCATGTCGATAAGGTTCGCATTAATCCCGGCCTGTATGTTT
TTGAAAAAGCGAAGGATGATCGGACCGAATTTTCCCAATCTGAATTTGAAAATATTGGCGATAAAATTCGTGAAACCCTT
GAACCTTTAGTGGTTTCTCTCCGAGACCAAAACAAGGCCATGCGGATCGGGGTAAACCATGGCTCTTTGGCAGAGCGCAT
GTTGTTTACCTATGGGGATACCCCTGAAGGCATGGTGGAGTCCGCTCTAGAGTTTATTCGCATCTGCCAATCTCTGGATT
TCAATAATCTTGTGATTTCAATGAAAGCGTCTCGGGTGCCCGTGATGCTAGCTGCGTACCGCCTGATGGCCCGGCGAATG
GATGAGCTAGGCATGGATTATCCGCTTCATCTCGGTGTGACTGAAGCTGGAGATGGTGAATATGGCCGGATCAAATCTAC
TGCAGGGATTGCAACCCTATTGGCAGATGGGATTGGTGATACCCTGCGGGTGTCCCTGACCGAATCTCCAGAGAAAGAGA
TTCCCGTTTGCTACAGTATCCTGCAAGCCTTGGGCCTCCGCAAAACCATGGTGGAATACGTGGCTTGTCCCTCCTGTGGG
CGGACGCTCTTCAATTTGGAAGAAGTGCTTCATCAAGTGCGATCGGCGACGAACCATCTGACGGGTCTGGATATTGCCGT
TATGGGCTGTATTGTCAATGGCCCTGGTGAGATGGCTGATGCAGACTACGGCTATGTGGGTAAGCAACCTGGCTATATTT
CCCTATATCGGGGCCGAGAAGAAGTGAAGAAAGTTCCAGAGACTGAGGGGGTTGAAGAATTAATCAACCTGATTAAATCT
GACGGCCGTTGGGTGGATCCTGAATAG

Upstream 100 bases:

>100_bases
CCAAGAATAGACCGCCAAACGGGGATCGATCTATGGCAAGCTAAGGGGTGGAGCGTTTTTGCTCCCTGAGTTTCCTTATC
GTGGTCTTACCCCCTTCACC

Downstream 100 bases:

>100_bases
TTATCCGTTCAATTCTCTCCACTCTTGGAGCTGGTTTGAATCGATTGTTGAACAGTAGATTGGCCGACAAACGGTGCACC
CGTACCAAATTTGAATAGGT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTLSTPVTGTDERSTPVSTSITRRKTRPVPVGDVIIGGGHPVVVQSMINEDTLDIEGSVAAIRRLHELGCEIVRVTVPS
MAHAKALATIKEKLTATYQTVPLVADVHHNGMKIALEVAKHVDKVRINPGLYVFEKAKDDRTEFSQSEFENIGDKIRETL
EPLVVSLRDQNKAMRIGVNHGSLAERMLFTYGDTPEGMVESALEFIRICQSLDFNNLVISMKASRVPVMLAAYRLMARRM
DELGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTLRVSLTESPEKEIPVCYSILQALGLRKTMVEYVACPSCG
RTLFNLEEVLHQVRSATNHLTGLDIAVMGCIVNGPGEMADADYGYVGKQPGYISLYRGREEVKKVPETEGVEELINLIKS
DGRWVDPE

Sequences:

>Translated_408_residues
MQTLSTPVTGTDERSTPVSTSITRRKTRPVPVGDVIIGGGHPVVVQSMINEDTLDIEGSVAAIRRLHELGCEIVRVTVPS
MAHAKALATIKEKLTATYQTVPLVADVHHNGMKIALEVAKHVDKVRINPGLYVFEKAKDDRTEFSQSEFENIGDKIRETL
EPLVVSLRDQNKAMRIGVNHGSLAERMLFTYGDTPEGMVESALEFIRICQSLDFNNLVISMKASRVPVMLAAYRLMARRM
DELGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTLRVSLTESPEKEIPVCYSILQALGLRKTMVEYVACPSCG
RTLFNLEEVLHQVRSATNHLTGLDIAVMGCIVNGPGEMADADYGYVGKQPGYISLYRGREEVKKVPETEGVEELINLIKS
DGRWVDPE
>Mature_408_residues
MQTLSTPVTGTDERSTPVSTSITRRKTRPVPVGDVIIGGGHPVVVQSMINEDTLDIEGSVAAIRRLHELGCEIVRVTVPS
MAHAKALATIKEKLTATYQTVPLVADVHHNGMKIALEVAKHVDKVRINPGLYVFEKAKDDRTEFSQSEFENIGDKIRETL
EPLVVSLRDQNKAMRIGVNHGSLAERMLFTYGDTPEGMVESALEFIRICQSLDFNNLVISMKASRVPVMLAAYRLMARRM
DELGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTLRVSLTESPEKEIPVCYSILQALGLRKTMVEYVACPSCG
RTLFNLEEVLHQVRSATNHLTGLDIAVMGCIVNGPGEMADADYGYVGKQPGYISLYRGREEVKKVPETEGVEELINLIKS
DGRWVDPE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:
GO:0005506: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0008299: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0016114: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0016491: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0046429: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0046872: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0051536: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0051539: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
GO:0055114: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=396, Percent_Identity=37.6262626262626, Blast_Score=240, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_ACAM1 (B0C6E1)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514549.1
- ProteinModelPortal:   B0C6E1
- SMR:   B0C6E1
- GeneID:   5678985
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0149
- HOGENOM:   HBG335271
- OMA:   INEDTMD
- ProtClustDB:   PRK00366
- BioCyc:   AMAR329726:AM1_0149-MONOMER
- HAMAP:   MF_00159
- InterPro:   IPR016425
- InterPro:   IPR004588
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 44795; Mature: 44795

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTLSTPVTGTDERSTPVSTSITRRKTRPVPVGDVIIGGGHPVVVQSMINEDTLDIEGSV
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHH
AAIRRLHELGCEIVRVTVPSMAHAKALATIKEKLTATYQTVPLVADVHHNGMKIALEVAK
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHH
HVDKVRINPGLYVFEKAKDDRTEFSQSEFENIGDKIRETLEPLVVSLRDQNKAMRIGVNH
HHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GSLAERMLFTYGDTPEGMVESALEFIRICQSLDFNNLVISMKASRVPVMLAAYRLMARRM
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
DELGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTLRVSLTESPEKEIPVCYSI
HHHCCCCCEEECCEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHH
LQALGLRKTMVEYVACPSCGRTLFNLEEVLHQVRSATNHLTGLDIAVMGCIVNGPGEMAD
HHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCC
ADYGYVGKQPGYISLYRGREEVKKVPETEGVEELINLIKSDGRWVDPE
CCCCCCCCCCCEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTLSTPVTGTDERSTPVSTSITRRKTRPVPVGDVIIGGGHPVVVQSMINEDTLDIEGSV
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCHH
AAIRRLHELGCEIVRVTVPSMAHAKALATIKEKLTATYQTVPLVADVHHNGMKIALEVAK
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHH
HVDKVRINPGLYVFEKAKDDRTEFSQSEFENIGDKIRETLEPLVVSLRDQNKAMRIGVNH
HHHHEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GSLAERMLFTYGDTPEGMVESALEFIRICQSLDFNNLVISMKASRVPVMLAAYRLMARRM
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
DELGMDYPLHLGVTEAGDGEYGRIKSTAGIATLLADGIGDTLRVSLTESPEKEIPVCYSI
HHHCCCCCEEECCEECCCCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHH
LQALGLRKTMVEYVACPSCGRTLFNLEEVLHQVRSATNHLTGLDIAVMGCIVNGPGEMAD
HHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEECCCCCCCC
ADYGYVGKQPGYISLYRGREEVKKVPETEGVEELINLIKSDGRWVDPE
CCCCCCCCCCCEEEEECCHHHHHCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA