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The map label for this gene is ndhJ

Identifier: 158333361

GI number: 158333361

Start: 132432

End: 132980

Strand: Direct

Name: ndhJ

Synonym: AM1_0133

Alternate gene names: 158333361

Gene position: 132432-132980 (Clockwise)

Preceding gene: 158333360

Following gene: 158333366

Centisome position: 2.04

GC content: 47.91

Gene sequence:

>549_bases
GTGGCAGATTCCTCTTCCGAATCAACCCAAGACGAACAATCTGTTGCAGAAGAACAAGCAGCACCCATTATTGAAGCAGG
TCCTGTCGCCAAGCATCTTACCCAAGCAGGTTTCACGGTTGAAGCCCTGGGTGCCGATCATCTCGGCGTAGAAATGATCA
AAGTGACCGCTGACCAGCTGGTGGATGTGGCAACTACTTTGGTCAATGACGGATATAACTATCTCATGTGCCAAGGCGGA
TATGATTCTGGGCCAGGTCAAGAGTTGGTCAGTATGTATCATCTTCTGAAGCTCAGTGATGATGCAGACCGTCCACCAGA
AGTCAGAATTAAAGTTTTTCTCTCTCGAGAAGATCCCCGCGTTCCCTCTCTATATTGGGTCTGGAAAGCCTCTGACTGGC
AAGAGCGTGAGTCCTATGATATGTATGGCATCGTCTATGAAGGTCACCCAAATCTGACTCGTATCCTTATGCCTGAAGAT
TGGGTTGGCTGGCCCTTGCGCAAAGATTACATCAGTCCTGATTTCTATGAACTCCAGGATGCTTATTAA

Upstream 100 bases:

>100_bases
AAAAGGAGCCTTGGAGTGGTCCTAGTTAGGTTGGATATCCACTCTTCCTCTTTCTAAAAACTTCTTCTTCATTTTCTAGT
GCATATCAAAGGAGCAAACG

Downstream 100 bases:

>100_bases
GCCCTTCTAATTTTCGTTCAAAATGAAAGCCTCGCTGATATAACCAAGCGGGGCTTTCATTTCTCAAAAATGTAGCAATA
TTAGTCGTCGCGGCGGCCAG

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit J; NADH-plastoquinone oxidoreductase subunit J; NDH-1 subunit J; NDH-J

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MADSSSESTQDEQSVAEEQAAPIIEAGPVAKHLTQAGFTVEALGADHLGVEMIKVTADQLVDVATTLVNDGYNYLMCQGG
YDSGPGQELVSMYHLLKLSDDADRPPEVRIKVFLSREDPRVPSLYWVWKASDWQERESYDMYGIVYEGHPNLTRILMPED
WVGWPLRKDYISPDFYELQDAY

Sequences:

>Translated_182_residues
MADSSSESTQDEQSVAEEQAAPIIEAGPVAKHLTQAGFTVEALGADHLGVEMIKVTADQLVDVATTLVNDGYNYLMCQGG
YDSGPGQELVSMYHLLKLSDDADRPPEVRIKVFLSREDPRVPSLYWVWKASDWQERESYDMYGIVYEGHPNLTRILMPED
WVGWPLRKDYISPDFYELQDAY
>Mature_181_residues
ADSSSESTQDEQSVAEEQAAPIIEAGPVAKHLTQAGFTVEALGADHLGVEMIKVTADQLVDVATTLVNDGYNYLMCQGGY
DSGPGQELVSMYHLLKLSDDADRPPEVRIKVFLSREDPRVPSLYWVWKASDWQERESYDMYGIVYEGHPNLTRILMPEDW
VGWPLRKDYISPDFYELQDAY

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0852

COG function: function code C; NADH:ubiquinone oxidoreductase 27 kD subunit

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 30 kDa subunit family

Homologues:

Organism=Escherichia coli, GI145693162, Length=159, Percent_Identity=32.0754716981132, Blast_Score=87, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NDHJ_ACAM1 (B0C6C5)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001514533.1
- ProteinModelPortal:   B0C6C5
- SMR:   B0C6C5
- GeneID:   5678969
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0133
- HOGENOM:   HBG727675
- OMA:   YELQDAY
- ProtClustDB:   PRK12494
- BioCyc:   AMAR329726:AM1_0133-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01357
- InterPro:   IPR010218
- InterPro:   IPR001268
- ProDom:   PD001581

Pfam domain/function: PF00329 Complex1_30kDa

EC number: 1.6.5.3

Molecular weight: Translated: 20553; Mature: 20422

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSSSESTQDEQSVAEEQAAPIIEAGPVAKHLTQAGFTVEALGADHLGVEMIKVTADQL
CCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHEEHHHHHH
VDVATTLVNDGYNYLMCQGGYDSGPGQELVSMYHLLKLSDDADRPPEVRIKVFLSREDPR
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC
VPSLYWVWKASDWQERESYDMYGIVYEGHPNLTRILMPEDWVGWPLRKDYISPDFYELQD
CCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCHHCCCCCCHHHHCC
AY
CC
>Mature Secondary Structure 
ADSSSESTQDEQSVAEEQAAPIIEAGPVAKHLTQAGFTVEALGADHLGVEMIKVTADQL
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHEEHHHHHH
VDVATTLVNDGYNYLMCQGGYDSGPGQELVSMYHLLKLSDDADRPPEVRIKVFLSREDPR
HHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCC
VPSLYWVWKASDWQERESYDMYGIVYEGHPNLTRILMPEDWVGWPLRKDYISPDFYELQD
CCEEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCHHCCCCCCHHHHCC
AY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA