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The map label for this gene is zraS [H]

Identifier: 158333248

GI number: 158333248

Start: 16489

End: 18813

Strand: Reverse

Name: zraS [H]

Synonym: AM1_0017

Alternate gene names: 158333248

Gene position: 18813-16489 (Counterclockwise)

Preceding gene: 158333249

Following gene: 158333247

Centisome position: 0.29

GC content: 47.05

Gene sequence:

>2325_bases
ATGTTTATTCAAACGCTGCGATCCTTCCCTGGAAAACTCGCTCGTCGCCACAAGCTCAGAACGGTCCTGGTTGTACCTTT
TGTATTGCAGGTTTGCGGTGCAGTTGGTCTGGTCGGTTATTTATCATTTCAGAATGGTCAACGATCAATTGAATCCCTGG
CTCAGCAGCTGACAGATGAAGTGGAAGTCCATATTGCTGAGCGGACCCAAACCTATTTGGCTGCTCCCCATGCCGTTAAC
CAGCTTAATCAGAATGCTTTAGAACTCGGGCAGCTGAGCGCCGATAACCTCAACAGTATGGAACGCCATTTTTGGCGACA
GATCCAGGTATTTGATCGGATTAGCTATATTCAGTTTGGGACGCCCAAAGGAGAATTTGTCGGGCTGGCAGCGAATGATG
ATGGTAGTTTTACTTATCAGGTAACGGAGTATTCTGGGGATTTACAAACCTATGCCATTAAACAAAATGGACAGCGGGGG
AAAAAATTAGAAACATCTCCTAATTTTGAACCCCAAAAACGTCCTTGGTATTTAATTCCCGAAAAAAAAGATAAGCCGAC
TTGGACCGATATTTATGCTTGGGTTAACCCTCCGACTTTAGCCATTACCTTAGGGCAACCTTATTATGATCAGTCCGGAA
CCTTTCAAGGTATCTTAGCAACGGATCTATCGATTGCTCAGTTTAGCGATTTTCTGCAAACCCTCAAGATTGGTAAAACA
GGCAAGAGTTTTATCCTGGAGCGCTCAGGATTAATGGTTGCTACTTCTACGGCCGAGGAGCCCTTTACGCCTTCCGAGAA
AGGTCCACAGCGTTTACCAGCCCTGGAAAGCAAAGATCCCGTTGTCCGGGCAACCACTCAAACATTATTGGATCGGTTTG
GGACTTTGCAGGAGATTTCGGTTCGCCAACAGCTCACCATCGATATTGAGGGCAAGCGTTATTTTCTCCAGGTGAATCCC
CTTCGAGATCAGCATGGTTTAGATTGGCTCAGTGTGGTTGTTGTTCCAGAAGCAGACTTTATGGAACAAATTTATGCCAA
TACCCGCTCAACCATTGGGCTATCGGTTGCTGCGCTGCTATTTGCGATCCTCATTGGGGTGTCTACCGCCCGGTGGGTGA
CGAATCCCATCTTGAAAATCAACGCTGCTGCTACGGCTATCTCTTCAGGAGATTTGCAGCGAAGTGTTGCTCTCAACCGC
TCGGATGAGTTAGGAGAATTAGCACGATCGTTTAATCAAATGGTTCAAGATCTGCGCATCTCCTTGGAAGAACGAAATGT
GGTGGTAGAGGCACTCAAAAAGAGTGAACAACAGTTAGCAGAATATAACCGGTCTCTAGAACAGGAGGTTCAACAGCGAA
CCCAGGAACTGGTTCAATCTGAAAAAATGGTGGCGTTAGGCCAACTCACCGCAGGGGTTGCCCATGAAATCAATACGCCC
TTGGGGGCGATTCAAGCTGCCGGAGAAAATATTGCATCTTCTCTAGAGAAGTCCATGCAGCAGCTCCCCAAAATCCTGGA
TCAATTACAGCCAGAACAAACCGACGCATTTTTTGAGTTATTGGCGATGGCTCAGCAACAGGAGCTGTTAACCTCCCGCG
AGGAGCGTAAGCTGAAACGAAGTTTGAAGAAAATCCTGGCAGAGAAAAGCTTAGAGCCTGCCAATGATATGGCTAGTACC
CTTAGTAAGATGGGGGTCTCTGCTGATCTTTCGTCGTTTCTACCCATTCTGGAGTTGCCGAACAATACCGAAGTTCTGGA
TGTTGCCTATGATTTGACGGTCATACATAACAACACTCAGAATATCCGCTTGGCTGTAGGACGGGCAGCCAAAATTGTGT
TTGCTCTGAAGAGCTATGCTCGCCAAGATCTGAGTAACGAGAAAGTCAAAGCGTCAGTTACAGACACGGTAGAGACGGTT
CTGACCTTGTACCATAACCAGATTAAACACGGTATCGACTTGACTAAAGACTTTGAGGATGTCCCAGATATTCTGTGCTA
TCCAGAAGAACTGGCTCAGGTGTGGACGAATCTGCTGCACAATGCCATGCAGGCTATGGATTACAAAGGCCACATTCAAT
TAACCATTGGCCAGGAGCAGCAGGGGATTCTGGTCAGCTTTACGGATTCAGGCAGTGGTATTCCTGAGGATGTGCAAGCC
AAGATCTTTGATCCCTTTTTCACGACAAAACCTGCTGGCGAAGGGAGTGGTTTGGGACTAGATATCGTCCGTAAAATTGT
CGATAAACACCAAGGGAAGGTCGAATGTCAGAGTCAGCCAGGGCATACTAGGTTTGAGGTCTGGTTACCCCTTGAGGTGA
GCTAA

Upstream 100 bases:

>100_bases
GCAACTTCATGCTTATATTTCGAAGCCATGGAATGAAGAAGATTTGATGGGTGTGATTCAAGCTGGACTGTCTACATTTC
AAGAATAGAGAAAGGGATTG

Downstream 100 bases:

>100_bases
TCAGTTGAAAATGTGCTTGAATATCAGGTTAATCCTCATTGTATTTGAACGGAATGTGCCCAAAGTGACGACAGCGACAA
TGGTGGAGCAACGGTGAGCA

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MFIQTLRSFPGKLARRHKLRTVLVVPFVLQVCGAVGLVGYLSFQNGQRSIESLAQQLTDEVEVHIAERTQTYLAAPHAVN
QLNQNALELGQLSADNLNSMERHFWRQIQVFDRISYIQFGTPKGEFVGLAANDDGSFTYQVTEYSGDLQTYAIKQNGQRG
KKLETSPNFEPQKRPWYLIPEKKDKPTWTDIYAWVNPPTLAITLGQPYYDQSGTFQGILATDLSIAQFSDFLQTLKIGKT
GKSFILERSGLMVATSTAEEPFTPSEKGPQRLPALESKDPVVRATTQTLLDRFGTLQEISVRQQLTIDIEGKRYFLQVNP
LRDQHGLDWLSVVVVPEADFMEQIYANTRSTIGLSVAALLFAILIGVSTARWVTNPILKINAAATAISSGDLQRSVALNR
SDELGELARSFNQMVQDLRISLEERNVVVEALKKSEQQLAEYNRSLEQEVQQRTQELVQSEKMVALGQLTAGVAHEINTP
LGAIQAAGENIASSLEKSMQQLPKILDQLQPEQTDAFFELLAMAQQQELLTSREERKLKRSLKKILAEKSLEPANDMAST
LSKMGVSADLSSFLPILELPNNTEVLDVAYDLTVIHNNTQNIRLAVGRAAKIVFALKSYARQDLSNEKVKASVTDTVETV
LTLYHNQIKHGIDLTKDFEDVPDILCYPEELAQVWTNLLHNAMQAMDYKGHIQLTIGQEQQGILVSFTDSGSGIPEDVQA
KIFDPFFTTKPAGEGSGLGLDIVRKIVDKHQGKVECQSQPGHTRFEVWLPLEVS

Sequences:

>Translated_774_residues
MFIQTLRSFPGKLARRHKLRTVLVVPFVLQVCGAVGLVGYLSFQNGQRSIESLAQQLTDEVEVHIAERTQTYLAAPHAVN
QLNQNALELGQLSADNLNSMERHFWRQIQVFDRISYIQFGTPKGEFVGLAANDDGSFTYQVTEYSGDLQTYAIKQNGQRG
KKLETSPNFEPQKRPWYLIPEKKDKPTWTDIYAWVNPPTLAITLGQPYYDQSGTFQGILATDLSIAQFSDFLQTLKIGKT
GKSFILERSGLMVATSTAEEPFTPSEKGPQRLPALESKDPVVRATTQTLLDRFGTLQEISVRQQLTIDIEGKRYFLQVNP
LRDQHGLDWLSVVVVPEADFMEQIYANTRSTIGLSVAALLFAILIGVSTARWVTNPILKINAAATAISSGDLQRSVALNR
SDELGELARSFNQMVQDLRISLEERNVVVEALKKSEQQLAEYNRSLEQEVQQRTQELVQSEKMVALGQLTAGVAHEINTP
LGAIQAAGENIASSLEKSMQQLPKILDQLQPEQTDAFFELLAMAQQQELLTSREERKLKRSLKKILAEKSLEPANDMAST
LSKMGVSADLSSFLPILELPNNTEVLDVAYDLTVIHNNTQNIRLAVGRAAKIVFALKSYARQDLSNEKVKASVTDTVETV
LTLYHNQIKHGIDLTKDFEDVPDILCYPEELAQVWTNLLHNAMQAMDYKGHIQLTIGQEQQGILVSFTDSGSGIPEDVQA
KIFDPFFTTKPAGEGSGLGLDIVRKIVDKHQGKVECQSQPGHTRFEVWLPLEVS
>Mature_774_residues
MFIQTLRSFPGKLARRHKLRTVLVVPFVLQVCGAVGLVGYLSFQNGQRSIESLAQQLTDEVEVHIAERTQTYLAAPHAVN
QLNQNALELGQLSADNLNSMERHFWRQIQVFDRISYIQFGTPKGEFVGLAANDDGSFTYQVTEYSGDLQTYAIKQNGQRG
KKLETSPNFEPQKRPWYLIPEKKDKPTWTDIYAWVNPPTLAITLGQPYYDQSGTFQGILATDLSIAQFSDFLQTLKIGKT
GKSFILERSGLMVATSTAEEPFTPSEKGPQRLPALESKDPVVRATTQTLLDRFGTLQEISVRQQLTIDIEGKRYFLQVNP
LRDQHGLDWLSVVVVPEADFMEQIYANTRSTIGLSVAALLFAILIGVSTARWVTNPILKINAAATAISSGDLQRSVALNR
SDELGELARSFNQMVQDLRISLEERNVVVEALKKSEQQLAEYNRSLEQEVQQRTQELVQSEKMVALGQLTAGVAHEINTP
LGAIQAAGENIASSLEKSMQQLPKILDQLQPEQTDAFFELLAMAQQQELLTSREERKLKRSLKKILAEKSLEPANDMAST
LSKMGVSADLSSFLPILELPNNTEVLDVAYDLTVIHNNTQNIRLAVGRAAKIVFALKSYARQDLSNEKVKASVTDTVETV
LTLYHNQIKHGIDLTKDFEDVPDILCYPEELAQVWTNLLHNAMQAMDYKGHIQLTIGQEQQGILVSFTDSGSGIPEDVQA
KIFDPFFTTKPAGEGSGLGLDIVRKIVDKHQGKVECQSQPGHTRFEVWLPLEVS

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=117, Percent_Identity=41.8803418803419, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790300, Length=160, Percent_Identity=32.5, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788549, Length=107, Percent_Identity=43.9252336448598, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=176, Percent_Identity=28.9772727272727, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 86328; Mature: 86328

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIQTLRSFPGKLARRHKLRTVLVVPFVLQVCGAVGLVGYLSFQNGQRSIESLAQQLTDE
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VEVHIAERTQTYLAAPHAVNQLNQNALELGQLSADNLNSMERHFWRQIQVFDRISYIQFG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEC
TPKGEFVGLAANDDGSFTYQVTEYSGDLQTYAIKQNGQRGKKLETSPNFEPQKRPWYLIP
CCCCCEEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECC
EKKDKPTWTDIYAWVNPPTLAITLGQPYYDQSGTFQGILATDLSIAQFSDFLQTLKIGKT
CCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHCCCC
GKSFILERSGLMVATSTAEEPFTPSEKGPQRLPALESKDPVVRATTQTLLDRFGTLQEIS
CHHHHEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHC
VRQQLTIDIEGKRYFLQVNPLRDQHGLDWLSVVVVPEADFMEQIYANTRSTIGLSVAALL
CCEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHH
FAILIGVSTARWVTNPILKINAAATAISSGDLQRSVALNRSDELGELARSFNQMVQDLRI
HHHHHHCCHHHHHHCCCEEEEHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SLEERNVVVEALKKSEQQLAEYNRSLEQEVQQRTQELVQSEKMVALGQLTAGVAHEINTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGAIQAAGENIASSLEKSMQQLPKILDQLQPEQTDAFFELLAMAQQQELLTSREERKLKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLKKILAEKSLEPANDMASTLSKMGVSADLSSFLPILELPNNTEVLDVAYDLTVIHNNTQ
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHCCHHHCCCCCCEEEEEEEEEEEEECCCC
NIRLAVGRAAKIVFALKSYARQDLSNEKVKASVTDTVETVLTLYHNQIKHGIDLTKDFED
EEEEEECHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VPDILCYPEELAQVWTNLLHNAMQAMDYKGHIQLTIGQEQQGILVSFTDSGSGIPEDVQA
CHHHEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHH
KIFDPFFTTKPAGEGSGLGLDIVRKIVDKHQGKVECQSQPGHTRFEVWLPLEVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MFIQTLRSFPGKLARRHKLRTVLVVPFVLQVCGAVGLVGYLSFQNGQRSIESLAQQLTDE
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VEVHIAERTQTYLAAPHAVNQLNQNALELGQLSADNLNSMERHFWRQIQVFDRISYIQFG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEC
TPKGEFVGLAANDDGSFTYQVTEYSGDLQTYAIKQNGQRGKKLETSPNFEPQKRPWYLIP
CCCCCEEEEEECCCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECC
EKKDKPTWTDIYAWVNPPTLAITLGQPYYDQSGTFQGILATDLSIAQFSDFLQTLKIGKT
CCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCEEHHHCCHHHHHHHHHHHHHCCCC
GKSFILERSGLMVATSTAEEPFTPSEKGPQRLPALESKDPVVRATTQTLLDRFGTLQEIS
CHHHHEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHC
VRQQLTIDIEGKRYFLQVNPLRDQHGLDWLSVVVVPEADFMEQIYANTRSTIGLSVAALL
CCEEEEEEECCCEEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHH
FAILIGVSTARWVTNPILKINAAATAISSGDLQRSVALNRSDELGELARSFNQMVQDLRI
HHHHHHCCHHHHHHCCCEEEEHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SLEERNVVVEALKKSEQQLAEYNRSLEQEVQQRTQELVQSEKMVALGQLTAGVAHEINTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LGAIQAAGENIASSLEKSMQQLPKILDQLQPEQTDAFFELLAMAQQQELLTSREERKLKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLKKILAEKSLEPANDMASTLSKMGVSADLSSFLPILELPNNTEVLDVAYDLTVIHNNTQ
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHCCHHHCCCCCCEEEEEEEEEEEEECCCC
NIRLAVGRAAKIVFALKSYARQDLSNEKVKASVTDTVETVLTLYHNQIKHGIDLTKDFED
EEEEEECHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VPDILCYPEELAQVWTNLLHNAMQAMDYKGHIQLTIGQEQQGILVSFTDSGSGIPEDVQA
CHHHEECHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCCCCHHHH
KIFDPFFTTKPAGEGSGLGLDIVRKIVDKHQGKVECQSQPGHTRFEVWLPLEVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]