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The map label for this gene is insQ [H]

Identifier: 158318974

GI number: 158318974

Start: 8859181

End: 8860380

Strand: Reverse

Name: insQ [H]

Synonym: Franean1_7257

Alternate gene names: 158318974

Gene position: 8860380-8859181 (Counterclockwise)

Preceding gene: 158318975

Following gene: 158318966

Centisome position: 98.65

GC content: 71.0

Gene sequence:

>1200_bases
GTGAGACGTTCCTTCAAGTTCCAGCTTCGCCCGACCGCCCGGCAGGCCGCGGCGCTGAGTGTGATGCTTGGCGACCATCG
GGCGCTGTACAACGCCGCGTTGCAGGAACGCCGCGACGCCTGGCGTCACCCGTCGAAGACCATGATCCGCTACGGCGACC
AGTCCGCCCAGTTGAAGGAGATCCGCGCCTGTGATCCGGACCAGGGCCGCTGGTCGTTCTCCTCGCAGCAGGCCACCCTG
CGCCGGCTCGACAAGGCAATGGCCGCGTTCTTCCGCCGGGTCAGGGCGGGTGCGGCGCCGGGCTACCCGCGGTTCAGGGG
CGCGGGCCGGTTCGACACGGTGGAGTGGCCGAAGGACGGTGATGGTTGCCGCTGGGACTCCCAGCCCGACCATCCCACCC
GGACCTGGGTCCGACTGCAGGGCATCGGACATGTCAGGGTCAACCAGCATCGGCCCGTGGCCGGCACGGTCAAGACGATC
AGCCTGAGGCGGGAAGGCCGCCGCTGGTATGTGCTGTTGTCCTGCGACGACGTACCCGCTGAGCCCGCCCCGGCCACCGG
GGCGGTGGTCGGGGCGGACCTGGGTGTGGCGTCGCTGGTAACCCTCTCCGACGGCCGTCACGTCGGGAACCCCCGTTACC
TCGCGGCGGCGGCCGGCCGGCTCGCCCGGGCGCAGCGGGAACTGGCCCGCAAGAAGCGCGGGTCCACCCGGCGCCGGAAG
GCGGTCGCCAAGGTCGCGGCGCTGCACGGCGCGGTGCGCCGCCAGCGGCTCGATCTTGCCCACAAGGCGGCCCTCGGGCT
GGTCCGTGAGCATGATCTGATCGCCGTCGAGGCGCTGCGGGTCACCAACATGACCCGCAGGGCCAGACCGAAGCCCGACC
CTGACCAGTCGGGGGCGTTCCTCCCGAACGGTCAGGCCGCCAAGTCCGGGCTGAACAAGTCGATCCTTGACGCGGGATGG
GGGGTGTTCCTCGCTGTGCTGCGCGCCAAGGCTGAAAGTGCCGGACGGGTGGTCGTCGAGGTCAACCCTGCCAACACCTC
CCGCACGTGTGCGGTCTGCGGGCACTGCCACGCCGACAACCGCAGGACACAGGCCGCGTTCACCTGTGTCGCCTGCGGGC
ATGCCGCGCACGCCGACGTGAACGCGGCGGTCAACATCCTTCGGGTCGGGCTGGCCCGTCAGGCCGACGAAGCGGCCTGA

Upstream 100 bases:

>100_bases
GGTTGTACTTCGTGGCCGCTGTCGGTGTGGTGTCCGTGGACACGGTCCGCCGGTACATCGACACCCAGTACGAGTGGCCG
TGGCGCATGGAGCACGCCCG

Downstream 100 bases:

>100_bases
GAAGCCGGCGGCTTCAACCGTCGGAGTAGTCACGTGCCCAGGGTAGATGCCGTGTCGACCGGATTTCCTGCCCCCGTGTG
CCGTGGGCGTGGGCTCACCC

Product: IS605 family transposase OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRRSFKFQLRPTARQAAALSVMLGDHRALYNAALQERRDAWRHPSKTMIRYGDQSAQLKEIRACDPDQGRWSFSSQQATL
RRLDKAMAAFFRRVRAGAAPGYPRFRGAGRFDTVEWPKDGDGCRWDSQPDHPTRTWVRLQGIGHVRVNQHRPVAGTVKTI
SLRREGRRWYVLLSCDDVPAEPAPATGAVVGADLGVASLVTLSDGRHVGNPRYLAAAAGRLARAQRELARKKRGSTRRRK
AVAKVAALHGAVRRQRLDLAHKAALGLVREHDLIAVEALRVTNMTRRARPKPDPDQSGAFLPNGQAAKSGLNKSILDAGW
GVFLAVLRAKAESAGRVVVEVNPANTSRTCAVCGHCHADNRRTQAAFTCVACGHAAHADVNAAVNILRVGLARQADEAA

Sequences:

>Translated_399_residues
MRRSFKFQLRPTARQAAALSVMLGDHRALYNAALQERRDAWRHPSKTMIRYGDQSAQLKEIRACDPDQGRWSFSSQQATL
RRLDKAMAAFFRRVRAGAAPGYPRFRGAGRFDTVEWPKDGDGCRWDSQPDHPTRTWVRLQGIGHVRVNQHRPVAGTVKTI
SLRREGRRWYVLLSCDDVPAEPAPATGAVVGADLGVASLVTLSDGRHVGNPRYLAAAAGRLARAQRELARKKRGSTRRRK
AVAKVAALHGAVRRQRLDLAHKAALGLVREHDLIAVEALRVTNMTRRARPKPDPDQSGAFLPNGQAAKSGLNKSILDAGW
GVFLAVLRAKAESAGRVVVEVNPANTSRTCAVCGHCHADNRRTQAAFTCVACGHAAHADVNAAVNILRVGLARQADEAA
>Mature_399_residues
MRRSFKFQLRPTARQAAALSVMLGDHRALYNAALQERRDAWRHPSKTMIRYGDQSAQLKEIRACDPDQGRWSFSSQQATL
RRLDKAMAAFFRRVRAGAAPGYPRFRGAGRFDTVEWPKDGDGCRWDSQPDHPTRTWVRLQGIGHVRVNQHRPVAGTVKTI
SLRREGRRWYVLLSCDDVPAEPAPATGAVVGADLGVASLVTLSDGRHVGNPRYLAAAAGRLARAQRELARKKRGSTRRRK
AVAKVAALHGAVRRQRLDLAHKAALGLVREHDLIAVEALRVTNMTRRARPKPDPDQSGAFLPNGQAAKSGLNKSILDAGW
GVFLAVLRAKAESAGRVVVEVNPANTSRTCAVCGHCHADNRRTQAAFTCVACGHAAHADVNAAVNILRVGLARQADEAA

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=373, Percent_Identity=33.2439678284182, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 43654; Mature: 43654

Theoretical pI: Translated: 11.68; Mature: 11.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSFKFQLRPTARQAAALSVMLGDHRALYNAALQERRDAWRHPSKTMIRYGDQSAQLKE
CCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHEEECCCHHHHHH
IRACDPDQGRWSFSSQQATLRRLDKAMAAFFRRVRAGAAPGYPRFRGAGRFDTVEWPKDG
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DGCRWDSQPDHPTRTWVRLQGIGHVRVNQHRPVAGTVKTISLRREGRRWYVLLSCDDVPA
CCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCEEEEEEEECCCCC
EPAPATGAVVGADLGVASLVTLSDGRHVGNPRYLAAAAGRLARAQRELARKKRGSTRRRK
CCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AVAKVAALHGAVRRQRLDLAHKAALGLVREHDLIAVEALRVTNMTRRARPKPDPDQSGAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCC
LPNGQAAKSGLNKSILDAGWGVFLAVLRAKAESAGRVVVEVNPANTSRTCAVCGHCHADN
CCCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCC
RRTQAAFTCVACGHAAHADVNAAVNILRVGLARQADEAA
CCHHHHEEEEECCCCHHCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRSFKFQLRPTARQAAALSVMLGDHRALYNAALQERRDAWRHPSKTMIRYGDQSAQLKE
CCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHEEECCCHHHHHH
IRACDPDQGRWSFSSQQATLRRLDKAMAAFFRRVRAGAAPGYPRFRGAGRFDTVEWPKDG
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DGCRWDSQPDHPTRTWVRLQGIGHVRVNQHRPVAGTVKTISLRREGRRWYVLLSCDDVPA
CCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCEEEEEEEECCCCC
EPAPATGAVVGADLGVASLVTLSDGRHVGNPRYLAAAAGRLARAQRELARKKRGSTRRRK
CCCCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AVAKVAALHGAVRRQRLDLAHKAALGLVREHDLIAVEALRVTNMTRRARPKPDPDQSGAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCC
LPNGQAAKSGLNKSILDAGWGVFLAVLRAKAESAGRVVVEVNPANTSRTCAVCGHCHADN
CCCCHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCCCC
RRTQAAFTCVACGHAAHADVNAAVNILRVGLARQADEAA
CCHHHHEEEEECCCCHHCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]