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The map label for this gene is vdh [H]

Identifier: 158318924

GI number: 158318924

Start: 8799955

End: 8801055

Strand: Direct

Name: vdh [H]

Synonym: Franean1_7207

Alternate gene names: 158318924

Gene position: 8799955-8801055 (Clockwise)

Preceding gene: 158318920

Following gene: 158318925

Centisome position: 97.97

GC content: 74.21

Gene sequence:

>1101_bases
ATGTCAGCGGTGGTCGACACGTCCACGGGAGTCTCGTCGGTGTTCGAGGCCGGCGCGGAGCACGAGCAGGTGGTGTTCTG
CTCCGATCGGGCCAGTGGCCTGCGCGCCGTGATCGCCATCTACTCGACCGCGCTGGGGCCGGCGCTGGGAGGCACCCGGT
TCCACGCCTATCCGGACGAGGCGTCCGCACTAGCCGACGCCCTCGCGCTCTCCCGCGCGATGGCCTACAAGGCCGCCTGC
GCGGGCCTGGACCTCGGCGGCGGCAAGGCCGTCATCCTGGGCGACCCCGCCCGCGACAAGACCGAGGCGCTGCTGCGCGC
CTACGGGCGCTTCATCGCCTCGCTGGGCGGCCGCTACGTGACGGCCTGCGACGTCGGGACGTACGTCGAGGACATGGACA
CCATCGCCAGGGAGACCCGTTGGGTCACCGGCCGCTCGCCGGCGCACGGCGGCTCGGGCGACTCCGGCGTCCTGACCGCG
TACGGCGTCTTCGAGGGGATGCGCGCCTGCGCCCGGCACCGATGGGGGACACCCTCGCTCGCCGGGCGCCGGGTCGCCGT
CAGCGGGGTCGGCAAGGTCGGCCTGCGCCTCGTGGGGCACCTGGTGGAGGAGGGGGCGACCGTTCTGGCCGGGGATACCG
ATCCGGGCGCCCTGCGGCGACTGGGAGCCCGCCATCCCGACGTCCAGCTGGTGGCCGACCCCGACGAGCTCCTCCGGGCC
GAGGTCGACATCTACGCGCCCTGCGCGCTGGGCGGGGTGCTCACCGACGAGGTCGTGCCCGCGCTGCGGGCGGAGATCAT
CTGCGGCGGGGCGAACAACCAGCTGGCCCACCCGGGCATGGACAAGGTCCTGGCCGACGCGGGCGTGCTGTACGCGCCCG
ACTTCGTGGTCAACGCCGGCGGACTGATCCAGGTGGCGGACGAGATCGAGGGGTACTCCCCGGAACGGGCCAGGGCCCGG
GCCGCCCGGATCTTCGACACGGCGCTGGACATCTTCCGGCTCGCCGAGGCGGAGGGCGCCACCCCGGCGGTGGCGGCGGG
ACGCTTCGCCGAGCGCCGGATGACCGACATCGGCCGGCTGCGGGGCATCCTGCTGCCCTGA

Upstream 100 bases:

>100_bases
TTCGCCGCAGCGGCCGGCGGGAGCCCGCCGCGCACAGGGCTCACGGGCCGTCGGCTCACGGGCCGTCGGCTCGCGGAGCC
GTCCGGAGGGAGGCTCGGTC

Downstream 100 bases:

>100_bases
CCGTCACCGGCGGGCACGCCCGGAACCCGTGTGCCAGCACGCCTTTCCCACCCCCCGGAAGCCGTGACGCTGGGCACATC
GGGCACCGGCGCGCGGGCGT

Product: Glu/Leu/Phe/Val dehydrogenase dimerisation region

Products: 2-Oxoglutarate; H+; Nicotinamide adenine dinucleotide phosphate - reduced; ammonia [C]

Alternate protein names: ValDH [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSAVVDTSTGVSSVFEAGAEHEQVVFCSDRASGLRAVIAIYSTALGPALGGTRFHAYPDEASALADALALSRAMAYKAAC
AGLDLGGGKAVILGDPARDKTEALLRAYGRFIASLGGRYVTACDVGTYVEDMDTIARETRWVTGRSPAHGGSGDSGVLTA
YGVFEGMRACARHRWGTPSLAGRRVAVSGVGKVGLRLVGHLVEEGATVLAGDTDPGALRRLGARHPDVQLVADPDELLRA
EVDIYAPCALGGVLTDEVVPALRAEIICGGANNQLAHPGMDKVLADAGVLYAPDFVVNAGGLIQVADEIEGYSPERARAR
AARIFDTALDIFRLAEAEGATPAVAAGRFAERRMTDIGRLRGILLP

Sequences:

>Translated_366_residues
MSAVVDTSTGVSSVFEAGAEHEQVVFCSDRASGLRAVIAIYSTALGPALGGTRFHAYPDEASALADALALSRAMAYKAAC
AGLDLGGGKAVILGDPARDKTEALLRAYGRFIASLGGRYVTACDVGTYVEDMDTIARETRWVTGRSPAHGGSGDSGVLTA
YGVFEGMRACARHRWGTPSLAGRRVAVSGVGKVGLRLVGHLVEEGATVLAGDTDPGALRRLGARHPDVQLVADPDELLRA
EVDIYAPCALGGVLTDEVVPALRAEIICGGANNQLAHPGMDKVLADAGVLYAPDFVVNAGGLIQVADEIEGYSPERARAR
AARIFDTALDIFRLAEAEGATPAVAAGRFAERRMTDIGRLRGILLP
>Mature_365_residues
SAVVDTSTGVSSVFEAGAEHEQVVFCSDRASGLRAVIAIYSTALGPALGGTRFHAYPDEASALADALALSRAMAYKAACA
GLDLGGGKAVILGDPARDKTEALLRAYGRFIASLGGRYVTACDVGTYVEDMDTIARETRWVTGRSPAHGGSGDSGVLTAY
GVFEGMRACARHRWGTPSLAGRRVAVSGVGKVGLRLVGHLVEEGATVLAGDTDPGALRRLGARHPDVQLVADPDELLRAE
VDIYAPCALGGVLTDEVVPALRAEIICGGANNQLAHPGMDKVLADAGVLYAPDFVVNAGGLIQVADEIEGYSPERARARA
ARIFDTALDIFRLAEAEGATPAVAAGRFAERRMTDIGRLRGILLP

Specific function: Oxidative deamination of branched-chain amino acids. Oxidizes L-valine and L-alpha-aminobutyric acid efficiently, and L-valine and L-isoleucine less efficiently. D-valine and L- glutamate were not substrates for the enzyme. The catabolism of valine is the

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=304, Percent_Identity=26.9736842105263, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4885281, Length=304, Percent_Identity=26.6447368421053, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI45549226, Length=305, Percent_Identity=29.5081967213115, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45553475, Length=305, Percent_Identity=29.5081967213115, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24649460, Length=270, Percent_Identity=29.2592592592593, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24649283, Length=327, Percent_Identity=25.9938837920489, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: 1.4.1.4 [C]

Molecular weight: Translated: 38056; Mature: 37924

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVVDTSTGVSSVFEAGAEHEQVVFCSDRASGLRAVIAIYSTALGPALGGTRFHAYPDE
CCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCH
ASALADALALSRAMAYKAACAGLDLGGGKAVILGDPARDKTEALLRAYGRFIASLGGRYV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEE
TACDVGTYVEDMDTIARETRWVTGRSPAHGGSGDSGVLTAYGVFEGMRACARHRWGTPSL
EEECHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCC
AGRRVAVSGVGKVGLRLVGHLVEEGATVLAGDTDPGALRRLGARHPDVQLVADPDELLRA
CCCEEEECCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCEEEECHHHHHHH
EVDIYAPCALGGVLTDEVVPALRAEIICGGANNQLAHPGMDKVLADAGVLYAPDFVVNAG
HCCEECCHHHCCHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHCCCCEECCCCEECCC
GLIQVADEIEGYSPERARARAARIFDTALDIFRLAEAEGATPAVAAGRFAERRMTDIGRL
CEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RGILLP
HCCCCC
>Mature Secondary Structure 
SAVVDTSTGVSSVFEAGAEHEQVVFCSDRASGLRAVIAIYSTALGPALGGTRFHAYPDE
CCCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCH
ASALADALALSRAMAYKAACAGLDLGGGKAVILGDPARDKTEALLRAYGRFIASLGGRYV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEE
TACDVGTYVEDMDTIARETRWVTGRSPAHGGSGDSGVLTAYGVFEGMRACARHRWGTPSL
EEECHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCC
AGRRVAVSGVGKVGLRLVGHLVEEGATVLAGDTDPGALRRLGARHPDVQLVADPDELLRA
CCCEEEECCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCEEEECHHHHHHH
EVDIYAPCALGGVLTDEVVPALRAEIICGGANNQLAHPGMDKVLADAGVLYAPDFVVNAG
HCCEECCHHHCCHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHCCCCEECCCCEECCC
GLIQVADEIEGYSPERARARAARIFDTALDIFRLAEAEGATPAVAAGRFAERRMTDIGRL
CEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RGILLP
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADP+; NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Glutamate; H2O; Nicotinamide adenine dinucleotide phosphate [C]

Specific reaction: L-Glutamate + H2O + Nicotinamide adenine dinucleotide phosphate <==> 2-Oxoglutarate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced + ammonia [C]

General reaction: Oxidative deamination; Redox reaction; Reductive amination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10612726; 11386345 [H]