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The map label for this gene is garR [C]

Identifier: 158318871

GI number: 158318871

Start: 8734112

End: 8734954

Strand: Reverse

Name: garR [C]

Synonym: Franean1_7152

Alternate gene names: 158318871

Gene position: 8734954-8734112 (Counterclockwise)

Preceding gene: 158318872

Following gene: 158318870

Centisome position: 97.25

GC content: 72.12

Gene sequence:

>843_bases
ATGAAGATCGGATTCGTCGGGCCGGGCCGGATGGGCCGGCCGATACTCGACAGGCTGACAGCCGCCGGCCACGACGTCAC
GGTGCTGGTGCGCCGCCCGGAGGCCAGGGCAGCGGCCGAGGCCGACGGGCTGCGCTGGGCGGCGACCGTGGCGGAGACCG
TCCATGACGCCGACGCGGTGTTCACCGTGGTGCTTCGGGACGACCAGGTCAGGCAGGTGTGCCTCGGCCCCGATGGGGCC
ATCGCCGCCATGAGGCCGGGAGCGACGCTCGTCCAGCACACGACCTCTGACCCCGCGACCGCGCGCCTGCTCGCCGACCA
GGCAGCGGACCGGGGAATACGAGTCCTTGACGCCGCCCTCTCAGGTGGTCCGCATGACATCGCCGCCGGCCGCCTCACCC
TGTGGGTGGGCGGCGACGAGGCGCTGCTGGACGAGATGCGACCGGTCCTCGGGGCGTACGCGTCGCCGATCATGTTCGTG
GGGGAGCTGGGTAACGGTCAGCGGGTCAAGCTCGTCAACAACGCGCTGTTCGTCGCGCAGGTCGGACTCGCGATCGACGC
GGTGCGCCTGGCGGCTTCGCTGGGAATCGAGGAGACGGCGATCCTGGCGGCCGTTCAGCACGGCAGCGGCGCCAGCCGCG
CCCTCGCGATCGTCGCCGGTGGCGGGTCGGTGGACGCGGTCCCCGGGCGCATCGGGGACCTCATGCTCAAGGATGTCACC
GTGGTGCGCGAGGTCGCCCGGAGCACAGGCGTGGACCTCGGGGTCATCGGGACGGTGCTGGCCTCCGACGCCGTCGAGAC
GAAAGTGCTCCGCCAGACCGGTGCACCCGCCCGGACGTCATGA

Upstream 100 bases:

>100_bases
CACACCCGCCTGCCGGTGGCGACTGACCCGCCCTCGGTGACCGGCCTGTCGGCGGTGACCCGCCGGTCGAGCGCTAGTAC
ACACGCCGTCTGGAGGATCA

Downstream 100 bases:

>100_bases
CCACCAGTACGCCGAGAGCTACCACGACGAGGACACCGTGAAGACTGGATTCATCGGGCTCGGCAGCATGGGGCTGCCGA
TGGCACAGCGGCTTCAGTCG

Product: NAD-binding 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKIGFVGPGRMGRPILDRLTAAGHDVTVLVRRPEARAAAEADGLRWAATVAETVHDADAVFTVVLRDDQVRQVCLGPDGA
IAAMRPGATLVQHTTSDPATARLLADQAADRGIRVLDAALSGGPHDIAAGRLTLWVGGDEALLDEMRPVLGAYASPIMFV
GELGNGQRVKLVNNALFVAQVGLAIDAVRLAASLGIEETAILAAVQHGSGASRALAIVAGGGSVDAVPGRIGDLMLKDVT
VVREVARSTGVDLGVIGTVLASDAVETKVLRQTGAPARTS

Sequences:

>Translated_280_residues
MKIGFVGPGRMGRPILDRLTAAGHDVTVLVRRPEARAAAEADGLRWAATVAETVHDADAVFTVVLRDDQVRQVCLGPDGA
IAAMRPGATLVQHTTSDPATARLLADQAADRGIRVLDAALSGGPHDIAAGRLTLWVGGDEALLDEMRPVLGAYASPIMFV
GELGNGQRVKLVNNALFVAQVGLAIDAVRLAASLGIEETAILAAVQHGSGASRALAIVAGGGSVDAVPGRIGDLMLKDVT
VVREVARSTGVDLGVIGTVLASDAVETKVLRQTGAPARTS
>Mature_280_residues
MKIGFVGPGRMGRPILDRLTAAGHDVTVLVRRPEARAAAEADGLRWAATVAETVHDADAVFTVVLRDDQVRQVCLGPDGA
IAAMRPGATLVQHTTSDPATARLLADQAADRGIRVLDAALSGGPHDIAAGRLTLWVGGDEALLDEMRPVLGAYASPIMFV
GELGNGQRVKLVNNALFVAQVGLAIDAVRLAASLGIEETAILAAVQHGSGASRALAIVAGGGSVDAVPGRIGDLMLKDVT
VVREVARSTGVDLGVIGTVLASDAVETKVLRQTGAPARTS

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=258, Percent_Identity=28.2945736434109, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI40556376, Length=211, Percent_Identity=31.2796208530806, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI145693186, Length=261, Percent_Identity=29.8850574712644, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786719, Length=217, Percent_Identity=29.9539170506912, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790315, Length=255, Percent_Identity=28.2352941176471, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789092, Length=273, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=261, Percent_Identity=25.2873563218391, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24655230, Length=232, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922568, Length=232, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28574115, Length=207, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 28733; Mature: 28733

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFVGPGRMGRPILDRLTAAGHDVTVLVRRPEARAAAEADGLRWAATVAETVHDADAV
CEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCEE
FTVVLRDDQVRQVCLGPDGAIAAMRPGATLVQHTTSDPATARLLADQAADRGIRVLDAAL
EEEEECCCCHHEEEECCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEHHHH
SGGPHDIAAGRLTLWVGGDEALLDEMRPVLGAYASPIMFVGELGNGQRVKLVNNALFVAQ
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCEEEEHH
VGLAIDAVRLAASLGIEETAILAAVQHGSGASRALAIVAGGGSVDAVPGRIGDLMLKDVT
HHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
VVREVARSTGVDLGVIGTVLASDAVETKVLRQTGAPARTS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIGFVGPGRMGRPILDRLTAAGHDVTVLVRRPEARAAAEADGLRWAATVAETVHDADAV
CEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCEE
FTVVLRDDQVRQVCLGPDGAIAAMRPGATLVQHTTSDPATARLLADQAADRGIRVLDAAL
EEEEECCCCHHEEEECCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEHHHH
SGGPHDIAAGRLTLWVGGDEALLDEMRPVLGAYASPIMFVGELGNGQRVKLVNNALFVAQ
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCEEEEHH
VGLAIDAVRLAASLGIEETAILAAVQHGSGASRALAIVAGGGSVDAVPGRIGDLMLKDVT
HHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
VVREVARSTGVDLGVIGTVLASDAVETKVLRQTGAPARTS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA