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The map label for this gene is gsiB [H]

Identifier: 158318867

GI number: 158318867

Start: 8729171

End: 8730766

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_7148

Alternate gene names: 158318867

Gene position: 8730766-8729171 (Counterclockwise)

Preceding gene: 158318868

Following gene: 158318866

Centisome position: 97.2

GC content: 67.04

Gene sequence:

>1596_bases
ATGACGCTCATGCCCATGGAAGGGTGGAAGATGAATCGTGGGACGCGATTATTCGTCACCGCTGCCGCATGCTGTGCGGC
GCTCCTCCTGGGGGCCTGCGGCGGCGGCGGCAGCGATCCCGCCACGTCCGCGGGGCCCTCGGGCGAGCCGGTGGCGGGTG
GCGAAGGACGGGTTCTCACGCTGAGTGATCCGCGTACTCTGGATCCGGCGATCCTCGGCAACGCGTATGCGTCCGGCGCC
TTCCTGGGCAACGCCCTGTACGGGACCCTGATGACCAACGATGAGGCAGACGAGGTCCATTACTCGATGGCCGAGTCGTT
CACCACGACCGACAACGGCAAGACCTTCACCCTGAAGCTGCGGCCGGGCCTGGTGTTCTCCGACGGCACTCCGCTGAACA
CCGAGGCCGTGAAGTTCAACTGGGACCGGACCAAGGACCCGGCCGTCGGCTCGGCCCACCGGTCGGAGGCGGCGACGATC
GAGTCGTCCGAGGTGGTGGACGACGTCACGCTGAAGGTTACCCTGGTCACCCCCGTGCCGAGGTACGCCCTGTCCGTCCT
CGCCTCCTCGATGAACTGGATCGCCTCACCCGCGGCCCTGCAGAAGGGCCAGCAGGCTTTCGACGCGAAGCCGATCGGCG
CGGGACCGTTCACCCTGGAGAGCTGGACCCGTCAGGCCGACATCCGGCTCGTCAAGAACCCCCGCTACTGGGACGCGCCC
AAGCCCTACCTCGACCGCCTCACCCTCCGCGCGGCGCTCGAAGGCAACCAGCGCTACAACACGCTGCTCACCGGGGGCGC
GGACGTGGCCATCGACTCGAGCTGGATCAACCTCGACAAGGCCACCAAGGCGGGTCTGCCGAAAAATGTCATGCGGCTCA
GCGGCGGCGTCTTCGCAGCGCTGAACATGCGCAGAGCACCGTTCGACGACATTCGTGCGCGTCGGGCGCTCGCGGCGGCA
CTGGACTCGGACGCGCTGAACCTCGCAGCGTACAACGGCACCGCGCAGCTGGCCGATACGTTGTTCACCGACGCCTCCCC
CTTCTACTCGGAGACGCCGCTGCGCAAGACGGACAAGGCGACCGCCCAGCGGCTCTTCGACGAGCTGGCCGCCGAAGGCA
AGCCACTGACCTTCACGTTCACCAGCTCCACCGCCAGCGAGAACAAGGCGCTTGCGGAGAACATCCAGGCCCAGCTCAGC
GCCTTCAGGAACGTCAAGGTCCAAATTAAGACCATTGAGCTCGCCGAGTTCGTCCAGCTGCGCAGGACGCTCGACTTCGA
CGCGGTTATCACGTCCGCGCTCTTCCAGGACCCCGAGCCTCGGTTGTCGACGGTTTTCGCCGGGGGCTCGCCGGCGAACC
TGGCCGGTATCAACGACCCGGCGCTCAACGCGGCCCTGCAGACCGGCCGGACCGCGACCTCGGAGGAGGAGCGCAAGGCG
GCCTACGACACCGTGCAGGAACGGCTGACCGAACTGACCCCGATGTTCTTCATCGCGCGTGGGGGGCTCGGCGCCATCTC
GGGCAAGAACGTCGGCGGCCTCGTGCAGTACGGCCTTGGTTCCCTGCTTCCCGAGGAACTGTGGATCCAGCCGTAA

Upstream 100 bases:

>100_bases
TGCTACGGGCAAAGAGTTGACCGAATGCGCAAAGACCTCCACGGTCCCACCGCCGATGATGTGTCGAGACGGCATCGCCG
GTAAATCCAGGAGGGCAGGG

Downstream 100 bases:

>100_bases
CCGTCCACCCTGGATGAGAGGTCGCCGTAGCCCACGGACGCTGGGGCGATCTCCCAGACTCGGACCAGGCTCGGCACCGG
GGGGAGGGTCCCTGGCCGGC

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTLMPMEGWKMNRGTRLFVTAAACCAALLLGACGGGGSDPATSAGPSGEPVAGGEGRVLTLSDPRTLDPAILGNAYASGA
FLGNALYGTLMTNDEADEVHYSMAESFTTTDNGKTFTLKLRPGLVFSDGTPLNTEAVKFNWDRTKDPAVGSAHRSEAATI
ESSEVVDDVTLKVTLVTPVPRYALSVLASSMNWIASPAALQKGQQAFDAKPIGAGPFTLESWTRQADIRLVKNPRYWDAP
KPYLDRLTLRAALEGNQRYNTLLTGGADVAIDSSWINLDKATKAGLPKNVMRLSGGVFAALNMRRAPFDDIRARRALAAA
LDSDALNLAAYNGTAQLADTLFTDASPFYSETPLRKTDKATAQRLFDELAAEGKPLTFTFTSSTASENKALAENIQAQLS
AFRNVKVQIKTIELAEFVQLRRTLDFDAVITSALFQDPEPRLSTVFAGGSPANLAGINDPALNAALQTGRTATSEEERKA
AYDTVQERLTELTPMFFIARGGLGAISGKNVGGLVQYGLGSLLPEELWIQP

Sequences:

>Translated_531_residues
MTLMPMEGWKMNRGTRLFVTAAACCAALLLGACGGGGSDPATSAGPSGEPVAGGEGRVLTLSDPRTLDPAILGNAYASGA
FLGNALYGTLMTNDEADEVHYSMAESFTTTDNGKTFTLKLRPGLVFSDGTPLNTEAVKFNWDRTKDPAVGSAHRSEAATI
ESSEVVDDVTLKVTLVTPVPRYALSVLASSMNWIASPAALQKGQQAFDAKPIGAGPFTLESWTRQADIRLVKNPRYWDAP
KPYLDRLTLRAALEGNQRYNTLLTGGADVAIDSSWINLDKATKAGLPKNVMRLSGGVFAALNMRRAPFDDIRARRALAAA
LDSDALNLAAYNGTAQLADTLFTDASPFYSETPLRKTDKATAQRLFDELAAEGKPLTFTFTSSTASENKALAENIQAQLS
AFRNVKVQIKTIELAEFVQLRRTLDFDAVITSALFQDPEPRLSTVFAGGSPANLAGINDPALNAALQTGRTATSEEERKA
AYDTVQERLTELTPMFFIARGGLGAISGKNVGGLVQYGLGSLLPEELWIQP
>Mature_530_residues
TLMPMEGWKMNRGTRLFVTAAACCAALLLGACGGGGSDPATSAGPSGEPVAGGEGRVLTLSDPRTLDPAILGNAYASGAF
LGNALYGTLMTNDEADEVHYSMAESFTTTDNGKTFTLKLRPGLVFSDGTPLNTEAVKFNWDRTKDPAVGSAHRSEAATIE
SSEVVDDVTLKVTLVTPVPRYALSVLASSMNWIASPAALQKGQQAFDAKPIGAGPFTLESWTRQADIRLVKNPRYWDAPK
PYLDRLTLRAALEGNQRYNTLLTGGADVAIDSSWINLDKATKAGLPKNVMRLSGGVFAALNMRRAPFDDIRARRALAAAL
DSDALNLAAYNGTAQLADTLFTDASPFYSETPLRKTDKATAQRLFDELAAEGKPLTFTFTSSTASENKALAENIQAQLSA
FRNVKVQIKTIELAEFVQLRRTLDFDAVITSALFQDPEPRLSTVFAGGSPANLAGINDPALNAALQTGRTATSEEERKAA
YDTVQERLTELTPMFFIARGGLGAISGKNVGGLVQYGLGSLLPEELWIQP

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=411, Percent_Identity=27.7372262773723, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1787495, Length=543, Percent_Identity=24.1252302025783, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789397, Length=504, Percent_Identity=25.1984126984127, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789966, Length=476, Percent_Identity=24.5798319327731, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081878, Length=515, Percent_Identity=23.8834951456311, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787762, Length=475, Percent_Identity=25.0526315789474, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1789887, Length=475, Percent_Identity=25.2631578947368, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787551, Length=461, Percent_Identity=24.295010845987, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56701; Mature: 56569

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLMPMEGWKMNRGTRLFVTAAACCAALLLGACGGGGSDPATSAGPSGEPVAGGEGRVLT
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
LSDPRTLDPAILGNAYASGAFLGNALYGTLMTNDEADEVHYSMAESFTTTDNGKTFTLKL
ECCCCCCCHHHHCCCHHCCHHHHHHHEEEEECCCCHHHHHHHHHHHCEECCCCCEEEEEE
RPGLVFSDGTPLNTEAVKFNWDRTKDPAVGSAHRSEAATIESSEVVDDVTLKVTLVTPVP
CCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCHHHHHCCEEEEEEEECCCH
RYALSVLASSMNWIASPAALQKGQQAFDAKPIGAGPFTLESWTRQADIRLVKNPRYWDAP
HHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCC
KPYLDRLTLRAALEGNQRYNTLLTGGADVAIDSSWINLDKATKAGLPKNVMRLSGGVFAA
CHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCEECCCHHHHCCCCHHHHHHCCCEEEE
LNMRRAPFDDIRARRALAAALDSDALNLAAYNGTAQLADTLFTDASPFYSETPLRKTDKA
ECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHH
TAQRLFDELAAEGKPLTFTFTSSTASENKALAENIQAQLSAFRNVKVQIKTIELAEFVQL
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHH
RRTLDFDAVITSALFQDPEPRLSTVFAGGSPANLAGINDPALNAALQTGRTATSEEERKA
HHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
AYDTVQERLTELTPMFFIARGGLGAISGKNVGGLVQYGLGSLLPEELWIQP
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure 
TLMPMEGWKMNRGTRLFVTAAACCAALLLGACGGGGSDPATSAGPSGEPVAGGEGRVLT
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
LSDPRTLDPAILGNAYASGAFLGNALYGTLMTNDEADEVHYSMAESFTTTDNGKTFTLKL
ECCCCCCCHHHHCCCHHCCHHHHHHHEEEEECCCCHHHHHHHHHHHCEECCCCCEEEEEE
RPGLVFSDGTPLNTEAVKFNWDRTKDPAVGSAHRSEAATIESSEVVDDVTLKVTLVTPVP
CCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCHHCCCCHHHHHCCEEEEEEEECCCH
RYALSVLASSMNWIASPAALQKGQQAFDAKPIGAGPFTLESWTRQADIRLVKNPRYWDAP
HHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCCCCC
KPYLDRLTLRAALEGNQRYNTLLTGGADVAIDSSWINLDKATKAGLPKNVMRLSGGVFAA
CHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCEECCCHHHHCCCCHHHHHHCCCEEEE
LNMRRAPFDDIRARRALAAALDSDALNLAAYNGTAQLADTLFTDASPFYSETPLRKTDKA
ECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHH
TAQRLFDELAAEGKPLTFTFTSSTASENKALAENIQAQLSAFRNVKVQIKTIELAEFVQL
HHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHH
RRTLDFDAVITSALFQDPEPRLSTVFAGGSPANLAGINDPALNAALQTGRTATSEEERKA
HHHCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHH
AYDTVQERLTELTPMFFIARGGLGAISGKNVGGLVQYGLGSLLPEELWIQP
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA