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The map label for this gene is dmfA2 [H]

Identifier: 158318788

GI number: 158318788

Start: 8614358

End: 8616631

Strand: Reverse

Name: dmfA2 [H]

Synonym: Franean1_7060

Alternate gene names: 158318788

Gene position: 8616631-8614358 (Counterclockwise)

Preceding gene: 158318789

Following gene: 158318785

Centisome position: 95.93

GC content: 67.72

Gene sequence:

>2274_bases
GTGAGCGCGGAAGGCTGTTCTGTAACGTCGCCGGTCTGGGTTCTCGGATACTCTGACGAAATCATCGTGCGGCCCGGGCA
GGACCTGACCTTCCGGGTCGGCGGGCAGGGGGTCGACACGTTTCACGCCCACCTTGTCCGGTTGCGCCACGGCGACACCA
CTCCGGGTGGCCCGGGATTTCGCGAGACTGAGCTCGACTCCGAGGTCAACGGAGTTTATCCGCTATTGGACCAACCAGCG
CATGTCGGGTCGTTCGGCCGGATTCCCGGCGCCCGCGACATCCTGCGCTCGCCGGACGGTTCACTGACCCTTTTCGCCTT
CGTTCAGCCCACCGCCGACGGCGCCCGGCAGCCGGTGCTCTCCGGCTGGGACGACGCGACCTCGACGGGCGTCGCGCTGG
TTCTGACCGATGGGTTGCGTCCCGCTCTCGTGTTCGGCGCGGGCCAGGATTCCGCCTGCCTCACCGCGCCCACCGGCCTG
CAGGCCGGCACCTGGTACGCGCTGGCGGCAAGCTGGGACGGCGCGACCGGTCAGGCCAGCGTGACCGCGGTGCCGGTGGT
GAACGCGTACAACAGCCGGTTCGGACGGGTGGTGGCCAACCCCATGGTGGAGATCACCGGCGCCCTTGCTGGCCCACCCG
AGATCCCGGCCGTCGACCTGCTCCTCGGCGCTCTGGACACGACCGACGGCACTGTCGTGCGGGGGGCATACAACGGCAAG
ATCGCGCTTCCAGCGGTCTACCGACGGCCTTTGACCGGCGACGAGGTGCGCCAGTTGGCCGGCAGCGGTAAAGCACCGGC
GGTGATCGAAGGGCTCGGAGCCTGGTGGGACCTGTCCACCGGAATCGAGGGCACCGAGATCGCGCCCGGCGACCGCGGTG
AAGGTGAACGTGTCGCCGGCAGTCTGGTGAACATGCCCACTCGCGCCGTGACCGCCCACAACTGGGACGGTTCCTCATAC
AACTGGACGGCCCATCCCGAGTTGTACGGCGCGGTGCACTTTCACACCGACGACGTCGACGACACCGGCTGGGCGCCGAC
CTGCACGCTGTCGGTGGGCGCCGACCTGCCATCGGGCGTATACGCACTGCGGCTACGGGCGGGCGGCTTCGAGGACCATG
TGCCGTTCCTGGTCGGCGCCGCTCAGGGCGAGGAGAAATCGATCGCCCTGCTGCTGCCGACCGCGAGTTACCAGGCCTAC
GCCAACGAGCATCTGGGCAGCGGTTCACCGATCGGGCAGGCGGTCGGCGGGCACACGCCCCTGGTGCAGCCGCTGGACGT
GCTCAAGCTCGAACACGGCGAGTTCGGACGCTCGACCTACGACCTGCATGTCGACGGCAGCGGCGTCGCCTACACCTCGC
GGCGCCGGCCGATCCTGAACATGCGCCCGCGGTACCGGTTCACGCATCTGGGCACCTGGCAGTTCCCCGCGGACCTTTAT
CTCGTCGACTGGCTGACCGAGAAGGGATACGAGTTCGACGTCCTCTGTGACGAGGATGTCCACCGACGTGGCGTGGACGC
GATGCGGCCCTATCGGGTGGTCATGACCGGCACCCATCCCGAATACACCTCAGAACGGATGCTCGATTCCTTCGAGGACT
ACGTCGTCGATGGCGGGCGCGTGATGTACATGGGCGGCAACGGGTTCTACTGGGTCGTGTCCTTCCATCCGGAGAAGCCC
TGGGTCATGGAGGTCCGCAAGGGTGAGGCTGGTTCGCGGGCCTGGCAGGCCGCGCCCGGGGAGACCCTCCACTCCACCAC
CGGCGAGAAGGGCGGCCTGTGGCGAAACCGTGGCCGCGCTCCGCAGAAGGTCTTCGGCACCGGGTTCACAGCGGAGGGGT
TCAACGGGTCCTCGTACTACCGGCGGATGCCGGACAGCGCCGAGGCAGCGGTGTCCTGGGTCTTCTCCGGTGTCGGCGAG
GAGGAACTGATCGGTGACTTCGGCCTGGAGGGCGCCGGCGCCGCCGGCCAGGAACTGGACCGCGCCGACGTCCGGCTGGG
CACCCCACCGGGCACCTACCTGCTCGCCTCCTCGGAGTACCACGACGACTCCTACCTGCTGGTGGTAGAAGAGATCGGTT
TCATGTACCCGGGGCTCGGCGGCAGCGAGCACCGAGACGTCCGCGCGGACATGACCCTGTTCGAGACCGAATCAGGCGGC
GCTGTCTTTTCGACCTCGTCGATTGCCTGGGCGGGAAGCCTCTCTCACAACGACTACGACAACAACGTCGCGCGCATCAC
CGCCAACGTTCTCGATCGCTTCTCGACGTTGTGA

Upstream 100 bases:

>100_bases
GTCTGCGCACCGAACGTGTCGCCGGTCCCCACCCGACCGTGGGCGCGGCGGTGCATGCGGTTTTCCTGAGCAGGGTTCGG
CTGGTGTTCGGTGTGGCTGC

Downstream 100 bases:

>100_bases
TCCAGACGACACTCCTGGCGTCGAAGCATGCAGCCGCCAACCGAGACGGCGGGGTGGGACGACGCCGCGATCCGCGCCGT
GCACAGACCGATGACAGCGA

Product: large subunit of N,N-dimethylformamidase

Products: NA

Alternate protein names: N,N-dimethylformamidase heavy chain [H]

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MSAEGCSVTSPVWVLGYSDEIIVRPGQDLTFRVGGQGVDTFHAHLVRLRHGDTTPGGPGFRETELDSEVNGVYPLLDQPA
HVGSFGRIPGARDILRSPDGSLTLFAFVQPTADGARQPVLSGWDDATSTGVALVLTDGLRPALVFGAGQDSACLTAPTGL
QAGTWYALAASWDGATGQASVTAVPVVNAYNSRFGRVVANPMVEITGALAGPPEIPAVDLLLGALDTTDGTVVRGAYNGK
IALPAVYRRPLTGDEVRQLAGSGKAPAVIEGLGAWWDLSTGIEGTEIAPGDRGEGERVAGSLVNMPTRAVTAHNWDGSSY
NWTAHPELYGAVHFHTDDVDDTGWAPTCTLSVGADLPSGVYALRLRAGGFEDHVPFLVGAAQGEEKSIALLLPTASYQAY
ANEHLGSGSPIGQAVGGHTPLVQPLDVLKLEHGEFGRSTYDLHVDGSGVAYTSRRRPILNMRPRYRFTHLGTWQFPADLY
LVDWLTEKGYEFDVLCDEDVHRRGVDAMRPYRVVMTGTHPEYTSERMLDSFEDYVVDGGRVMYMGGNGFYWVVSFHPEKP
WVMEVRKGEAGSRAWQAAPGETLHSTTGEKGGLWRNRGRAPQKVFGTGFTAEGFNGSSYYRRMPDSAEAAVSWVFSGVGE
EELIGDFGLEGAGAAGQELDRADVRLGTPPGTYLLASSEYHDDSYLLVVEEIGFMYPGLGGSEHRDVRADMTLFETESGG
AVFSTSSIAWAGSLSHNDYDNNVARITANVLDRFSTL

Sequences:

>Translated_757_residues
MSAEGCSVTSPVWVLGYSDEIIVRPGQDLTFRVGGQGVDTFHAHLVRLRHGDTTPGGPGFRETELDSEVNGVYPLLDQPA
HVGSFGRIPGARDILRSPDGSLTLFAFVQPTADGARQPVLSGWDDATSTGVALVLTDGLRPALVFGAGQDSACLTAPTGL
QAGTWYALAASWDGATGQASVTAVPVVNAYNSRFGRVVANPMVEITGALAGPPEIPAVDLLLGALDTTDGTVVRGAYNGK
IALPAVYRRPLTGDEVRQLAGSGKAPAVIEGLGAWWDLSTGIEGTEIAPGDRGEGERVAGSLVNMPTRAVTAHNWDGSSY
NWTAHPELYGAVHFHTDDVDDTGWAPTCTLSVGADLPSGVYALRLRAGGFEDHVPFLVGAAQGEEKSIALLLPTASYQAY
ANEHLGSGSPIGQAVGGHTPLVQPLDVLKLEHGEFGRSTYDLHVDGSGVAYTSRRRPILNMRPRYRFTHLGTWQFPADLY
LVDWLTEKGYEFDVLCDEDVHRRGVDAMRPYRVVMTGTHPEYTSERMLDSFEDYVVDGGRVMYMGGNGFYWVVSFHPEKP
WVMEVRKGEAGSRAWQAAPGETLHSTTGEKGGLWRNRGRAPQKVFGTGFTAEGFNGSSYYRRMPDSAEAAVSWVFSGVGE
EELIGDFGLEGAGAAGQELDRADVRLGTPPGTYLLASSEYHDDSYLLVVEEIGFMYPGLGGSEHRDVRADMTLFETESGG
AVFSTSSIAWAGSLSHNDYDNNVARITANVLDRFSTL
>Mature_756_residues
SAEGCSVTSPVWVLGYSDEIIVRPGQDLTFRVGGQGVDTFHAHLVRLRHGDTTPGGPGFRETELDSEVNGVYPLLDQPAH
VGSFGRIPGARDILRSPDGSLTLFAFVQPTADGARQPVLSGWDDATSTGVALVLTDGLRPALVFGAGQDSACLTAPTGLQ
AGTWYALAASWDGATGQASVTAVPVVNAYNSRFGRVVANPMVEITGALAGPPEIPAVDLLLGALDTTDGTVVRGAYNGKI
ALPAVYRRPLTGDEVRQLAGSGKAPAVIEGLGAWWDLSTGIEGTEIAPGDRGEGERVAGSLVNMPTRAVTAHNWDGSSYN
WTAHPELYGAVHFHTDDVDDTGWAPTCTLSVGADLPSGVYALRLRAGGFEDHVPFLVGAAQGEEKSIALLLPTASYQAYA
NEHLGSGSPIGQAVGGHTPLVQPLDVLKLEHGEFGRSTYDLHVDGSGVAYTSRRRPILNMRPRYRFTHLGTWQFPADLYL
VDWLTEKGYEFDVLCDEDVHRRGVDAMRPYRVVMTGTHPEYTSERMLDSFEDYVVDGGRVMYMGGNGFYWVVSFHPEKPW
VMEVRKGEAGSRAWQAAPGETLHSTTGEKGGLWRNRGRAPQKVFGTGFTAEGFNGSSYYRRMPDSAEAAVSWVFSGVGEE
ELIGDFGLEGAGAAGQELDRADVRLGTPPGTYLLASSEYHDDSYLLVVEEIGFMYPGLGGSEHRDVRADMTLFETESGGA
VFSTSSIAWAGSLSHNDYDNNVARITANVLDRFSTL

Specific function: Hydrolyzes N,N-dimethylformamide, and to a lesser extent N,N-dimethylacetamide and N,N-diethylacetamide. Has no activity against the substituted amides N-methylformamide, N- ethylformamide, N-ethylformamide and N-methylacetamide or the unsubstituted amide

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985 [H]

Pfam domain/function: NA

EC number: =3.5.1.56 [H]

Molecular weight: Translated: 81131; Mature: 81000

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEGCSVTSPVWVLGYSDEIIVRPGQDLTFRVGGQGVDTFHAHLVRLRHGDTTPGGPGF
CCCCCCCCCCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHEEEEEEECCCCCCCCCCC
RETELDSEVNGVYPLLDQPAHVGSFGRIPGARDILRSPDGSLTLFAFVQPTADGARQPVL
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
SGWDDATSTGVALVLTDGLRPALVFGAGQDSACLTAPTGLQAGTWYALAASWDGATGQAS
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCE
VTAVPVVNAYNSRFGRVVANPMVEITGALAGPPEIPAVDLLLGALDTTDGTVVRGAYNGK
EEEEEEEECCCCCCCHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCE
IALPAVYRRPLTGDEVRQLAGSGKAPAVIEGLGAWWDLSTGIEGTEIAPGDRGEGERVAG
EEEHHHHCCCCCHHHHHHHHCCCCCCHHHHCCCCEEECCCCCCCCEECCCCCCCCHHHHH
SLVNMPTRAVTAHNWDGSSYNWTAHPELYGAVHFHTDDVDDTGWAPTCTLSVGADLPSGV
HHHCCCCCEEEEECCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCE
YALRLRAGGFEDHVPFLVGAAQGEEKSIALLLPTASYQAYANEHLGSGSPIGQAVGGHTP
EEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCHHHCCCCCC
LVQPLDVLKLEHGEFGRSTYDLHVDGSGVAYTSRRRPILNMRPRYRFTHLGTWQFPADLY
CCCCCHHEEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCEE
LVDWLTEKGYEFDVLCDEDVHRRGVDAMRPYRVVMTGTHPEYTSERMLDSFEDYVVDGGR
EEEEHHCCCCEEEEEECCHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEECCCE
VMYMGGNGFYWVVSFHPEKPWVMEVRKGEAGSRAWQAAPGETLHSTTGEKGGLWRNRGRA
EEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
PQKVFGTGFTAEGFNGSSYYRRMPDSAEAAVSWVFSGVGEEELIGDFGLEGAGAAGQELD
CHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
RADVRLGTPPGTYLLASSEYHDDSYLLVVEEIGFMYPGLGGSEHRDVRADMTLFETESGG
CCCEEECCCCCCEEEEECCCCCCCEEEEEECCCHHCCCCCCCCCCCEEECEEEEEECCCC
AVFSTSSIAWAGSLSHNDYDNNVARITANVLDRFSTL
EEEECCCEEEEECCCCCCCCCCCEEEEHHHHHHHHCC
>Mature Secondary Structure 
SAEGCSVTSPVWVLGYSDEIIVRPGQDLTFRVGGQGVDTFHAHLVRLRHGDTTPGGPGF
CCCCCCCCCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHEEEEEEECCCCCCCCCCC
RETELDSEVNGVYPLLDQPAHVGSFGRIPGARDILRSPDGSLTLFAFVQPTADGARQPVL
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCC
SGWDDATSTGVALVLTDGLRPALVFGAGQDSACLTAPTGLQAGTWYALAASWDGATGQAS
CCCCCCCCCCEEEEEECCCCEEEEEECCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCE
VTAVPVVNAYNSRFGRVVANPMVEITGALAGPPEIPAVDLLLGALDTTDGTVVRGAYNGK
EEEEEEEECCCCCCCHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCE
IALPAVYRRPLTGDEVRQLAGSGKAPAVIEGLGAWWDLSTGIEGTEIAPGDRGEGERVAG
EEEHHHHCCCCCHHHHHHHHCCCCCCHHHHCCCCEEECCCCCCCCEECCCCCCCCHHHHH
SLVNMPTRAVTAHNWDGSSYNWTAHPELYGAVHFHTDDVDDTGWAPTCTLSVGADLPSGV
HHHCCCCCEEEEECCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCE
YALRLRAGGFEDHVPFLVGAAQGEEKSIALLLPTASYQAYANEHLGSGSPIGQAVGGHTP
EEEEEECCCCCCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCHHHCCCCCC
LVQPLDVLKLEHGEFGRSTYDLHVDGSGVAYTSRRRPILNMRPRYRFTHLGTWQFPADLY
CCCCCHHEEECCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCEE
LVDWLTEKGYEFDVLCDEDVHRRGVDAMRPYRVVMTGTHPEYTSERMLDSFEDYVVDGGR
EEEEHHCCCCEEEEEECCHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEECCCE
VMYMGGNGFYWVVSFHPEKPWVMEVRKGEAGSRAWQAAPGETLHSTTGEKGGLWRNRGRA
EEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
PQKVFGTGFTAEGFNGSSYYRRMPDSAEAAVSWVFSGVGEEELIGDFGLEGAGAAGQELD
CHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
RADVRLGTPPGTYLLASSEYHDDSYLLVVEEIGFMYPGLGGSEHRDVRADMTLFETESGG
CCCEEECCCCCCEEEEECCCCCCCEEEEEECCCHHCCCCCCCCCCCEEECEEEEEECCCC
AVFSTSSIAWAGSLSHNDYDNNVARITANVLDRFSTL
EEEECCCEEEEECCCCCCCCCCCEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA