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The map label for this gene is chiA [H]

Identifier: 158318717

GI number: 158318717

Start: 8509551

End: 8511170

Strand: Direct

Name: chiA [H]

Synonym: Franean1_6988

Alternate gene names: 158318717

Gene position: 8509551-8511170 (Clockwise)

Preceding gene: 158318716

Following gene: 158318718

Centisome position: 94.74

GC content: 74.44

Gene sequence:

>1620_bases
ATGTCGCCTTTGCGAGGTCGGCACCGACGCCGTCGTCGTCAGATCATTTCGGGTACCGCGGCCCTGCTCACCGTTGGCGG
TGCGGTCGGCCTGGCCGTGGTGGTGCCCGGCACCGCGGACGCCGCCGGCCTCGACGCCGCGTACAGCCGCACCAACGACT
GGGGCACCGGCTACTCCGCCCAGTATCAGGTCACCAACTCCGCGGACTCGCCGGACGGCTTCACCCTCGAGTTCGACCTG
CCGGACGGTGCGACCCTCACCTCGCTGTGGAACGCCGCCTACCAGGTGGACGGCCGTCACGTGACCGTCACCCCGCCGGC
CTGGCAGACCACACTCGCGCCACGGGAATCGGTCGACGTCGGCTTCGTCATCGCCGCTCCGGGCGGCGCCACCGACCCCC
TCGGATGTCGGATCAACGGCGAGGACTGCACGCCCGGCTCCGGCAACGGCGACCCGGGCCCGGAGCCTGAACCCTCGGCC
ACCGGGCCGGCCGCACCGTCGTCCCCGCCGCCGAACGCGTCGCCGACCGACCCGGCCACGCCACCCGACACGGCGCCGCG
GCCCACCGCGGGCCCGGGCACCGGCCAGCCGAGCGGCGCTCCGACGAGCACCCCGAGCACGCCGACCACGCCGCCCGCTT
CGACCACCGCGCCGCCGGCACCGCCGGCACCACCGGCACCGCCGTCGAACGGCTCCAGCGGATCGGGCGGGTTCGCGCCG
TACGTCGACACCTCGCTGTACCCGCCGTTCGACCTGGTCGCGGCGGCGCGGACCGCCGGCCTGCGTGACGTCACGCTGGC
CTTCGTCGTGGCCGGTGGAGGCGGCTGTACGCCGAAGTGGGGCGGGGTCAGCGACCTCACCATGGACGGCGTGCCCGGCC
AGATCGGCCGGTTCCGTGAGCTGGGCGGCGACGTCCGGGTGTCGTTCGGCGGGGCGTCCGGAACCGAGCTCGCCAGTGCC
TGCGGCAGCGCGGGCGACCTGGCGGCCGCGTACCGCAAGGTGGTCGACGTCTACGGGGTGACCCGGCTCGACTTCGACGT
CGAGGGCGGCACGTTGCCGGACGTCGCCGCGAACACCCGGCGTGCCCAGGCGATCGCCCGGCTTCAGCGGGAGGCCGCGG
CCGGAGGCCGGCCACTGGAGGTCTCGTTCACGCTGCCGGTGCTGCCGTCCGGCCTGACCCAGGCCGGCGTGGACCTGCTG
GCCAACGCCCGGGAGAACGGCGTGACGGTGAACGCCGTCAACATCATGGCGATGGACTACGGCGACGGCGCCGCGCCGAA
CCCGGCGGGCCGGATGGGCCAGTACGCCATCGACGCCGCCACCGCGACCCAGGCCCAGGTCAAGGGCGTGTTCGAGCTGT
CCGACGCGCAGGCGTGGGGGCGGGTGGCCGTCACCCCGATGATCGGTGTGAACGACGTCGCCAGCGAGGTGTTCACCCTG
GCCGACGCGCGGCGGCTGGTGCGGTTCGCGTCCGAGGTCGACCTCGCCTGGCTGTCGATGTGGTCGCTGACCCGCGACCA
GCCCTGCCCCGGTGGGCCGGTGCCGTACGCGCAGCCGACCTGCGGCGGCATCGAGGCGCAGCCGTTCGATTTCACCCGCG
CCTTCAACGCCGCCCAGTGA

Upstream 100 bases:

>100_bases
GTCTTAAGAGCGTTTCTGCGCTGCCTCAGCAGGGCCGGTCGGCGGCGCGCCCGCAACTAGCGTCCTGCCGCAATTCGCCA
TTGCGGGAGGTATGTACGCC

Downstream 100 bases:

>100_bases
GAAGCCGCCCGGTGAGAAGCCGCTCAGTGAAGGGGACCGCCATGCCGAACCGCCGCACGTTCCTGCTGGCGGCGTCCGCC
GCCGTCGCGGGTACCGCCGG

Product: lysozyme

Products: NA

Alternate protein names: Chitinase; Lysozyme [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSPLRGRHRRRRRQIISGTAALLTVGGAVGLAVVVPGTADAAGLDAAYSRTNDWGTGYSAQYQVTNSADSPDGFTLEFDL
PDGATLTSLWNAAYQVDGRHVTVTPPAWQTTLAPRESVDVGFVIAAPGGATDPLGCRINGEDCTPGSGNGDPGPEPEPSA
TGPAAPSSPPPNASPTDPATPPDTAPRPTAGPGTGQPSGAPTSTPSTPTTPPASTTAPPAPPAPPAPPSNGSSGSGGFAP
YVDTSLYPPFDLVAAARTAGLRDVTLAFVVAGGGGCTPKWGGVSDLTMDGVPGQIGRFRELGGDVRVSFGGASGTELASA
CGSAGDLAAAYRKVVDVYGVTRLDFDVEGGTLPDVAANTRRAQAIARLQREAAAGGRPLEVSFTLPVLPSGLTQAGVDLL
ANARENGVTVNAVNIMAMDYGDGAAPNPAGRMGQYAIDAATATQAQVKGVFELSDAQAWGRVAVTPMIGVNDVASEVFTL
ADARRLVRFASEVDLAWLSMWSLTRDQPCPGGPVPYAQPTCGGIEAQPFDFTRAFNAAQ

Sequences:

>Translated_539_residues
MSPLRGRHRRRRRQIISGTAALLTVGGAVGLAVVVPGTADAAGLDAAYSRTNDWGTGYSAQYQVTNSADSPDGFTLEFDL
PDGATLTSLWNAAYQVDGRHVTVTPPAWQTTLAPRESVDVGFVIAAPGGATDPLGCRINGEDCTPGSGNGDPGPEPEPSA
TGPAAPSSPPPNASPTDPATPPDTAPRPTAGPGTGQPSGAPTSTPSTPTTPPASTTAPPAPPAPPAPPSNGSSGSGGFAP
YVDTSLYPPFDLVAAARTAGLRDVTLAFVVAGGGGCTPKWGGVSDLTMDGVPGQIGRFRELGGDVRVSFGGASGTELASA
CGSAGDLAAAYRKVVDVYGVTRLDFDVEGGTLPDVAANTRRAQAIARLQREAAAGGRPLEVSFTLPVLPSGLTQAGVDLL
ANARENGVTVNAVNIMAMDYGDGAAPNPAGRMGQYAIDAATATQAQVKGVFELSDAQAWGRVAVTPMIGVNDVASEVFTL
ADARRLVRFASEVDLAWLSMWSLTRDQPCPGGPVPYAQPTCGGIEAQPFDFTRAFNAAQ
>Mature_538_residues
SPLRGRHRRRRRQIISGTAALLTVGGAVGLAVVVPGTADAAGLDAAYSRTNDWGTGYSAQYQVTNSADSPDGFTLEFDLP
DGATLTSLWNAAYQVDGRHVTVTPPAWQTTLAPRESVDVGFVIAAPGGATDPLGCRINGEDCTPGSGNGDPGPEPEPSAT
GPAAPSSPPPNASPTDPATPPDTAPRPTAGPGTGQPSGAPTSTPSTPTTPPASTTAPPAPPAPPAPPSNGSSGSGGFAPY
VDTSLYPPFDLVAAARTAGLRDVTLAFVVAGGGGCTPKWGGVSDLTMDGVPGQIGRFRELGGDVRVSFGGASGTELASAC
GSAGDLAAAYRKVVDVYGVTRLDFDVEGGTLPDVAANTRRAQAIARLQREAAAGGRPLEVSFTLPVLPSGLTQAGVDLLA
NARENGVTVNAVNIMAMDYGDGAAPNPAGRMGQYAIDAATATQAQVKGVFELSDAQAWGRVAVTPMIGVNDVASEVFTLA
DARRLVRFASEVDLAWLSMWSLTRDQPCPGGPVPYAQPTCGGIEAQPFDFTRAFNAAQ

Specific function: Bifunctional enzyme with lysozyme/chitinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 chitin-binding type-3 domains [H]

Homologues:

Organism=Escherichia coli, GI1789736, Length=277, Percent_Identity=37.9061371841155, Blast_Score=192, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003610
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02839 CBM_5_12 [H]

EC number: =3.2.1.14; =3.2.1.17 [H]

Molecular weight: Translated: 54687; Mature: 54556

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLRGRHRRRRRQIISGTAALLTVGGAVGLAVVVPGTADAAGLDAAYSRTNDWGTGYSA
CCCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCEE
QYQVTNSADSPDGFTLEFDLPDGATLTSLWNAAYQVDGRHVTVTPPAWQTTLAPRESVDV
EEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHEEECCCEEEECCCCCCCCCCCCCCCCE
GFVIAAPGGATDPLGCRINGEDCTPGSGNGDPGPEPEPSATGPAAPSSPPPNASPTDPAT
EEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPDTAPRPTAGPGTGQPSGAPTSTPSTPTTPPASTTAPPAPPAPPAPPSNGSSGSGGFAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YVDTSLYPPFDLVAAARTAGLRDVTLAFVVAGGGGCTPKWGGVSDLTMDGVPGQIGRFRE
CCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCEECCCCCHHHHHHH
LGGDVRVSFGGASGTELASACGSAGDLAAAYRKVVDVYGVTRLDFDVEGGTLPDVAANTR
CCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHH
RAQAIARLQREAAAGGRPLEVSFTLPVLPSGLTQAGVDLLANARENGVTVNAVNIMAMDY
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
GDGAAPNPAGRMGQYAIDAATATQAQVKGVFELSDAQAWGRVAVTPMIGVNDVASEVFTL
CCCCCCCCCCCCCCEEEHHHHHHHHHHCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHH
ADARRLVRFASEVDLAWLSMWSLTRDQPCPGGPVPYAQPTCGGIEAQPFDFTRAFNAAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SPLRGRHRRRRRQIISGTAALLTVGGAVGLAVVVPGTADAAGLDAAYSRTNDWGTGYSA
CCCCHHHHHHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCEE
QYQVTNSADSPDGFTLEFDLPDGATLTSLWNAAYQVDGRHVTVTPPAWQTTLAPRESVDV
EEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHEEECCCEEEECCCCCCCCCCCCCCCCE
GFVIAAPGGATDPLGCRINGEDCTPGSGNGDPGPEPEPSATGPAAPSSPPPNASPTDPAT
EEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPDTAPRPTAGPGTGQPSGAPTSTPSTPTTPPASTTAPPAPPAPPAPPSNGSSGSGGFAP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YVDTSLYPPFDLVAAARTAGLRDVTLAFVVAGGGGCTPKWGGVSDLTMDGVPGQIGRFRE
CCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCEECCCCCHHHHHHH
LGGDVRVSFGGASGTELASACGSAGDLAAAYRKVVDVYGVTRLDFDVEGGTLPDVAANTR
CCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHH
RAQAIARLQREAAAGGRPLEVSFTLPVLPSGLTQAGVDLLANARENGVTVNAVNIMAMDY
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEEEC
GDGAAPNPAGRMGQYAIDAATATQAQVKGVFELSDAQAWGRVAVTPMIGVNDVASEVFTL
CCCCCCCCCCCCCCEEEHHHHHHHHHHCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHH
ADARRLVRFASEVDLAWLSMWSLTRDQPCPGGPVPYAQPTCGGIEAQPFDFTRAFNAAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2661540; 10419961; 10760150 [H]