Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 158318654

GI number: 158318654

Start: 8431407

End: 8432183

Strand: Direct

Name: paaF [C]

Synonym: Franean1_6922

Alternate gene names: 158318654

Gene position: 8431407-8432183 (Clockwise)

Preceding gene: 158318652

Following gene: 158318661

Centisome position: 93.87

GC content: 69.76

Gene sequence:

>777_bases
GTGTACGACATGCCGGAGGAGATCGAGGTCCGCGCGGACGGGCCGCTGAGGATCATCACGCTGAACCGGCCCGACGATCT
CAACGCGGTCAACCACCCCCTGCACGTCGGGCTGGCGAAGCTCTGGCCGCAGCTGAACGAGGACCGCTCGGCGCGGGCTG
CCGTGCTCACCGGGGCCGGCCGGGCGTTCTCCGCAGGAGGCGACTTCGCCTACCTCGACGAGCTGTTCAAGGACAAGGAG
CTGCGCGCCCTGTCCATCGCGCACGGGCGCGAGATCGTCCTCGGCATGGTTCGCTGCCGGATTCCCGTGGTCGCCGCGGT
GAACGGCCCGGCCGTCGGTCTCGGTTGCAGCCTGGTCGCGCTGAGCGACATCGTCTACATGGCGGAGAAGGCCTTCCTCG
CGGACCCGCACATCCAGGTCGGCCTGGTGGCCGCCGACGGCGGGCCGCTGACCTGGCCCCTGCACACCAGCATGCACCTG
GCCAAGGAGTACGCGTTCACCAGCGCGCGCATCTCCGCCGAGCGGGCCATCCAGATGGGGCTGGCCAACCACATCGCCGC
CGATCCGGTGGCCGAGGCGCTGGCGTGCGCGAAGCGCATCGCCGAGCTGCCGCAGCAGGCGGTGGAGACCACCAAGCGGC
TGCTCAACCTGCACGTCGAGCGCGCCGTGATCGCCTCGCTCGACTTCGCGACAACCGCGGAGGAGGCGTCGTTCCAGACC
GATGACTTCGCCTCCATCGTCGAGCGGCTCTCCGCGAAGCAGAAGCAGAAGAGCTGA

Upstream 100 bases:

>100_bases
CGGCGGTGGCCGCGGCGCCCGGCAGCACATCGTCGAGGGTCACGCACCGGGGCCGGCCTCCGACAACATCGCTACCCGGA
TCATCGATTGGACGTATGAC

Downstream 100 bases:

>100_bases
GGGGACGGCGGTCCCGGGCGGCATGCCCAAGACCGCCCCGGCTTCTCCGGAACCGGTCGGCCAGGACCCGGGCGCTCGAA
GCGCCCGGGTCCTTCTGATT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MYDMPEEIEVRADGPLRIITLNRPDDLNAVNHPLHVGLAKLWPQLNEDRSARAAVLTGAGRAFSAGGDFAYLDELFKDKE
LRALSIAHGREIVLGMVRCRIPVVAAVNGPAVGLGCSLVALSDIVYMAEKAFLADPHIQVGLVAADGGPLTWPLHTSMHL
AKEYAFTSARISAERAIQMGLANHIAADPVAEALACAKRIAELPQQAVETTKRLLNLHVERAVIASLDFATTAEEASFQT
DDFASIVERLSAKQKQKS

Sequences:

>Translated_258_residues
MYDMPEEIEVRADGPLRIITLNRPDDLNAVNHPLHVGLAKLWPQLNEDRSARAAVLTGAGRAFSAGGDFAYLDELFKDKE
LRALSIAHGREIVLGMVRCRIPVVAAVNGPAVGLGCSLVALSDIVYMAEKAFLADPHIQVGLVAADGGPLTWPLHTSMHL
AKEYAFTSARISAERAIQMGLANHIAADPVAEALACAKRIAELPQQAVETTKRLLNLHVERAVIASLDFATTAEEASFQT
DDFASIVERLSAKQKQKS
>Mature_258_residues
MYDMPEEIEVRADGPLRIITLNRPDDLNAVNHPLHVGLAKLWPQLNEDRSARAAVLTGAGRAFSAGGDFAYLDELFKDKE
LRALSIAHGREIVLGMVRCRIPVVAAVNGPAVGLGCSLVALSDIVYMAEKAFLADPHIQVGLVAADGGPLTWPLHTSMHL
AKEYAFTSARISAERAIQMGLANHIAADPVAEALACAKRIAELPQQAVETTKRLLNLHVERAVIASLDFATTAEEASFQT
DDFASIVERLSAKQKQKS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=246, Percent_Identity=32.520325203252, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI70995211, Length=255, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI116235487, Length=137, Percent_Identity=32.1167883211679, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4757968, Length=177, Percent_Identity=26.5536723163842, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=177, Percent_Identity=26.5536723163842, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=214, Percent_Identity=26.6355140186916, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI260275230, Length=214, Percent_Identity=26.6355140186916, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI25453481, Length=177, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538573, Length=177, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI260274832, Length=214, Percent_Identity=26.6355140186916, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757966, Length=177, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538581, Length=177, Percent_Identity=25.9887005649718, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221307494, Length=137, Percent_Identity=27.7372262773723, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=137, Percent_Identity=27.7372262773723, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=137, Percent_Identity=27.7372262773723, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=213, Percent_Identity=31.924882629108, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI221142681, Length=209, Percent_Identity=31.1004784688995, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=233, Percent_Identity=31.7596566523605, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=200, Percent_Identity=29, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=224, Percent_Identity=26.7857142857143, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=26.5917602996255, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=248, Percent_Identity=24.1935483870968, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323314, Length=214, Percent_Identity=25.7009345794392, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324754, Length=180, Percent_Identity=30, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24654903, Length=269, Percent_Identity=28.996282527881, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20129971, Length=239, Percent_Identity=30.1255230125523, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653477, Length=239, Percent_Identity=30.1255230125523, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920382, Length=243, Percent_Identity=27.1604938271605, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21357171, Length=208, Percent_Identity=28.3653846153846, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27839; Mature: 27839

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDMPEEIEVRADGPLRIITLNRPDDLNAVNHPLHVGLAKLWPQLNEDRSARAAVLTGAG
CCCCCCCEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCC
RAFSAGGDFAYLDELFKDKELRALSIAHGREIVLGMVRCRIPVVAAVNGPAVGLGCSLVA
CEECCCCCHHHHHHHHCCCCHHEEHHHCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
LSDIVYMAEKAFLADPHIQVGLVAADGGPLTWPLHTSMHLAKEYAFTSARISAERAIQMG
HHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LANHIAADPVAEALACAKRIAELPQQAVETTKRLLNLHVERAVIASLDFATTAEEASFQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHCCCCC
DDFASIVERLSAKQKQKS
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYDMPEEIEVRADGPLRIITLNRPDDLNAVNHPLHVGLAKLWPQLNEDRSARAAVLTGAG
CCCCCCCEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCC
RAFSAGGDFAYLDELFKDKELRALSIAHGREIVLGMVRCRIPVVAAVNGPAVGLGCSLVA
CEECCCCCHHHHHHHHCCCCHHEEHHHCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHHH
LSDIVYMAEKAFLADPHIQVGLVAADGGPLTWPLHTSMHLAKEYAFTSARISAERAIQMG
HHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LANHIAADPVAEALACAKRIAELPQQAVETTKRLLNLHVERAVIASLDFATTAEEASFQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCHHHCCCCC
DDFASIVERLSAKQKQKS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA