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The map label for this gene is tfdA [H]

Identifier: 158318648

GI number: 158318648

Start: 8424694

End: 8425527

Strand: Direct

Name: tfdA [H]

Synonym: Franean1_6916

Alternate gene names: 158318648

Gene position: 8424694-8425527 (Clockwise)

Preceding gene: 158318647

Following gene: 158318649

Centisome position: 93.79

GC content: 69.78

Gene sequence:

>834_bases
ATGGGTACCACCACCAGGAAGATCACTCCGAACACCGGCACGGAGTTCAGCGGGCTGAAGGGTACGCAGCTCGTCGACAA
GGGCGTCGCCGACGACGCCCTGGCAGCCCTGGAGGAGACCGGGGTCGTCGTCTTCAGCGAGGCTGACATCGACGACGAGA
GCCTGGTCGCCTTCGCCCGGCTGCTCGGCAACGTCCTGCCGCTCCCGATGGGCAGCCACAAGATCAAGGAAATCCAGCGG
ATCACCCGCGACGCGTCGCAGAGCAAGCTGGCGGCGTACCGCGAGGCCACGTTCTACTGGCACATCGACGGGACCACCGG
CGAGGTGCCGGACAAGGCCACGCTGCTCACGGCACGGCGGATCTCGGGCGACCCGGAGGGCGACACCGAGTTCGCCAACA
CCTACGCCGCGTACGAGGCGCTGTCCGACGAGGAGAAGGCGCAGCTGGAGGGCGTGCGGGCCCTGCACAGCTTCACCGCC
TCCCAGCTCGTGGCCAACCCGAACCCCTCACCCGAGGAGCGGGCGGCCTGGGATCGCAACCCGACCCGGGAGCAGCCGAT
CGTGTGGACCCGGCGCAACGGCCGCAAGTCGCTGCTGGTCGGCTCGACCGCGGGCGAGGTCGTCGGCCTGCCCGCGGACG
AGGGGCGCGCCCTGCTCGACCGCCTGCTGGACTGGGCGACCCAGCCGCAGTTCGTCCTCCGGCACCGCTGGACCCAGGGC
GATCTCGTGATCTGGGACAACACCGGGATGCTGCACCGTGCCCTCCCCTACGGACCGTCATCGAGCCGGTTGATGCACCG
TGCATCGCTGGCCGGTGAGGAAGCGGTCGCGTAA

Upstream 100 bases:

>100_bases
CGTACCCGGTGAACATCTTCAAGCAGCCCCGGATCCCGGACTCCGGCGCGGCTTTCTTCGACACCGCCGTCGAACCGGCC
CTCTGAGAAGGCGACCTGCC

Downstream 100 bases:

>100_bases
TTCTGTTTGTGCGGAGTTCGCGCCCGTGGATCGCTGACTAGGCGGTTCACGGGCGCTCGCGCCGTCGACCGATCATTAGC
GACGCAGCTAGCAGAGGGAG

Product: taurine catabolism dioxygenase TauD/TfdA

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MGTTTRKITPNTGTEFSGLKGTQLVDKGVADDALAALEETGVVVFSEADIDDESLVAFARLLGNVLPLPMGSHKIKEIQR
ITRDASQSKLAAYREATFYWHIDGTTGEVPDKATLLTARRISGDPEGDTEFANTYAAYEALSDEEKAQLEGVRALHSFTA
SQLVANPNPSPEERAAWDRNPTREQPIVWTRRNGRKSLLVGSTAGEVVGLPADEGRALLDRLLDWATQPQFVLRHRWTQG
DLVIWDNTGMLHRALPYGPSSSRLMHRASLAGEEAVA

Sequences:

>Translated_277_residues
MGTTTRKITPNTGTEFSGLKGTQLVDKGVADDALAALEETGVVVFSEADIDDESLVAFARLLGNVLPLPMGSHKIKEIQR
ITRDASQSKLAAYREATFYWHIDGTTGEVPDKATLLTARRISGDPEGDTEFANTYAAYEALSDEEKAQLEGVRALHSFTA
SQLVANPNPSPEERAAWDRNPTREQPIVWTRRNGRKSLLVGSTAGEVVGLPADEGRALLDRLLDWATQPQFVLRHRWTQG
DLVIWDNTGMLHRALPYGPSSSRLMHRASLAGEEAVA
>Mature_276_residues
GTTTRKITPNTGTEFSGLKGTQLVDKGVADDALAALEETGVVVFSEADIDDESLVAFARLLGNVLPLPMGSHKIKEIQRI
TRDASQSKLAAYREATFYWHIDGTTGEVPDKATLLTARRISGDPEGDTEFANTYAAYEALSDEEKAQLEGVRALHSFTAS
QLVANPNPSPEERAAWDRNPTREQPIVWTRRNGRKSLLVGSTAGEVVGLPADEGRALLDRLLDWATQPQFVLRHRWTQGD
LVIWDNTGMLHRALPYGPSSSRLMHRASLAGEEAVA

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=271, Percent_Identity=28.4132841328413, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 30292; Mature: 30161

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTTRKITPNTGTEFSGLKGTQLVDKGVADDALAALEETGVVVFSEADIDDESLVAFAR
CCCCCCEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
LLGNVLPLPMGSHKIKEIQRITRDASQSKLAAYREATFYWHIDGTTGEVPDKATLLTARR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHEEEHHH
ISGDPEGDTEFANTYAAYEALSDEEKAQLEGVRALHSFTASQLVANPNPSPEERAAWDRN
HCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
PTREQPIVWTRRNGRKSLLVGSTAGEVVGLPADEGRALLDRLLDWATQPQFVLRHRWTQG
CCCCCCEEEEECCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHCCCCC
DLVIWDNTGMLHRALPYGPSSSRLMHRASLAGEEAVA
CEEEECCCCCEECCCCCCCCHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
GTTTRKITPNTGTEFSGLKGTQLVDKGVADDALAALEETGVVVFSEADIDDESLVAFAR
CCCCCEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
LLGNVLPLPMGSHKIKEIQRITRDASQSKLAAYREATFYWHIDGTTGEVPDKATLLTARR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCHHEEEHHH
ISGDPEGDTEFANTYAAYEALSDEEKAQLEGVRALHSFTASQLVANPNPSPEERAAWDRN
HCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
PTREQPIVWTRRNGRKSLLVGSTAGEVVGLPADEGRALLDRLLDWATQPQFVLRHRWTQG
CCCCCCEEEEECCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHCCCCC
DLVIWDNTGMLHRALPYGPSSSRLMHRASLAGEEAVA
CEEEECCCCCEECCCCCCCCHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]