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The map label for this gene is pcm [H]

Identifier: 158318484

GI number: 158318484

Start: 8212786

End: 8213994

Strand: Reverse

Name: pcm [H]

Synonym: Franean1_6752

Alternate gene names: 158318484

Gene position: 8213994-8212786 (Counterclockwise)

Preceding gene: 158318485

Following gene: 158318482

Centisome position: 91.45

GC content: 70.39

Gene sequence:

>1209_bases
TTGAGTACCGTGACCTCCACGCACGACGCCACCCCCGATGACCTGCGCGCGGCCATGGTCGACCGCCTCGCCACCTCGGG
AGCCATCCTCACCGCCGCGGTCGAGGACACGATGCGCACCGTGCCCCGCCACCTGTTCGTGCCCGACGCCGCCCCCGGCG
AGGCCTACGCCGAGCAGGCCGTCATCACCAAACGCGCCCCTGACGGCACATCCCTCAGTTACGCCTCCGGGCCGGGAATC
GTGGCGATGATGCTGGAGCAGCTCATCGTCCTACCCGGCCAGCGGATCCTCGAGATCGGGACCGGCACCGGCTACAACGC
CGCCCTCCTCGCGCACCTGGCCGGGCCCGGCGGGCACGTCACCACCATCGACATCGACCCCGACATCACCAGTGCTGCGA
CCAGCGCCCTCGCCGCAGCCGGCTTCGAAAAGGTCACGGTCCTCACCGGGGACGGCACCTTCGGCGACCCGGACAGCCAC
GTGCACGATCGGCTGATCGCCACGGTGGGAGTCTGGGACATCTCCAGCGCCTGGTGGGACCAGCTCGCCCCGGGCGGCCG
GCTCGTCCTGCCCCTGCATTGGCGGGGCCAGACCCGCGCGGTGACCTTCCGCCACGACGGCAACCGGATGATCAGCGAGT
CGGTAGAGCTCTGCGGCTCCGTCCCGATGATCGGGCAAGCGGGCGAACGGACCGCCGCGATCCACCATGATGGCCTCGTC
GCCCTGCACTGGGACCAGGACCAAGCCATCGACCCGGGCACTCTCACCGGCGTACTCGACAGGAGGCGGACAATCGCCCT
CTCCGGGGTCGAGGTCGGCCCCTACGACCCGTTCGACGGAATCTGGCTGCGCCTGACCGCCACCGAGCCCGGATGCTGCC
GCATCGAAGCCACCCCCGAAGCGGTGAAGTCCGCCCTGTGCGCACCCGCGATACCCCAGCGCAGCCCCGCTCTCGTCGAC
CGCGACTCGCTGGCCTACCTCAGCCTCGCCCGCAACCACACCGGGCCCGGCCGTCACACCCTCAGCGTCATCAGCCACGG
CCCGAACCGTCATAACCTGGCCGACCGGCTGATCGAAGGCATCCACACCTGGAACAGCGACCGCACCGCCACCCCCACCG
TCACCGCCCACCACGGGCCCGCCGGTCTCGCGCACCAGCCGCCCGGACTTATCCGGAAACCGGACAGCCCTCTGACGATT
ACCTTCTGA

Upstream 100 bases:

>100_bases
TGGGGTTCCGCATGCCCACCGGTGCCAGCTGTCCGCCATGCCCGTCCGGCCGCTGTCTGGCCTTCTTCGATCGCACCACA
CCCTTCCGGAAGGATGATCG

Downstream 100 bases:

>100_bases
TCTGCGCAGCCAAGACGCGGCCTCCGCACCCGGCCCTCTCCGTAGGGCCGCCGGTGGCTATCCCGTCCTCGCGCACGTCG
AGGGCGGTCACACCGATCCG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSTVTSTHDATPDDLRAAMVDRLATSGAILTAAVEDTMRTVPRHLFVPDAAPGEAYAEQAVITKRAPDGTSLSYASGPGI
VAMMLEQLIVLPGQRILEIGTGTGYNAALLAHLAGPGGHVTTIDIDPDITSAATSALAAAGFEKVTVLTGDGTFGDPDSH
VHDRLIATVGVWDISSAWWDQLAPGGRLVLPLHWRGQTRAVTFRHDGNRMISESVELCGSVPMIGQAGERTAAIHHDGLV
ALHWDQDQAIDPGTLTGVLDRRRTIALSGVEVGPYDPFDGIWLRLTATEPGCCRIEATPEAVKSALCAPAIPQRSPALVD
RDSLAYLSLARNHTGPGRHTLSVISHGPNRHNLADRLIEGIHTWNSDRTATPTVTAHHGPAGLAHQPPGLIRKPDSPLTI
TF

Sequences:

>Translated_402_residues
MSTVTSTHDATPDDLRAAMVDRLATSGAILTAAVEDTMRTVPRHLFVPDAAPGEAYAEQAVITKRAPDGTSLSYASGPGI
VAMMLEQLIVLPGQRILEIGTGTGYNAALLAHLAGPGGHVTTIDIDPDITSAATSALAAAGFEKVTVLTGDGTFGDPDSH
VHDRLIATVGVWDISSAWWDQLAPGGRLVLPLHWRGQTRAVTFRHDGNRMISESVELCGSVPMIGQAGERTAAIHHDGLV
ALHWDQDQAIDPGTLTGVLDRRRTIALSGVEVGPYDPFDGIWLRLTATEPGCCRIEATPEAVKSALCAPAIPQRSPALVD
RDSLAYLSLARNHTGPGRHTLSVISHGPNRHNLADRLIEGIHTWNSDRTATPTVTAHHGPAGLAHQPPGLIRKPDSPLTI
TF
>Mature_401_residues
STVTSTHDATPDDLRAAMVDRLATSGAILTAAVEDTMRTVPRHLFVPDAAPGEAYAEQAVITKRAPDGTSLSYASGPGIV
AMMLEQLIVLPGQRILEIGTGTGYNAALLAHLAGPGGHVTTIDIDPDITSAATSALAAAGFEKVTVLTGDGTFGDPDSHV
HDRLIATVGVWDISSAWWDQLAPGGRLVLPLHWRGQTRAVTFRHDGNRMISESVELCGSVPMIGQAGERTAAIHHDGLVA
LHWDQDQAIDPGTLTGVLDRRRTIALSGVEVGPYDPFDGIWLRLTATEPGCCRIEATPEAVKSALCAPAIPQRSPALVDR
DSLAYLSLARNHTGPGRHTLSVISHGPNRHNLADRLIEGIHTWNSDRTATPTVTAHHGPAGLAHQPPGLIRKPDSPLTIT
F

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=207, Percent_Identity=32.8502415458937, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 42547; Mature: 42416

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVTSTHDATPDDLRAAMVDRLATSGAILTAAVEDTMRTVPRHLFVPDAAPGEAYAEQA
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCEEECCCCCCCHHHHHHH
VITKRAPDGTSLSYASGPGIVAMMLEQLIVLPGQRILEIGTGTGYNAALLAHLAGPGGHV
HEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEE
TTIDIDPDITSAATSALAAAGFEKVTVLTGDGTFGDPDSHVHDRLIATVGVWDISSAWWD
EEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHEEEEEECCHHHHH
QLAPGGRLVLPLHWRGQTRAVTFRHDGNRMISESVELCGSVPMIGQAGERTAAIHHDGLV
HCCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEE
ALHWDQDQAIDPGTLTGVLDRRRTIALSGVEVGPYDPFDGIWLRLTATEPGCCRIEATPE
EEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCHH
AVKSALCAPAIPQRSPALVDRDSLAYLSLARNHTGPGRHTLSVISHGPNRHNLADRLIEG
HHHHHHCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
IHTWNSDRTATPTVTAHHGPAGLAHQPPGLIRKPDSPLTITF
HHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure 
STVTSTHDATPDDLRAAMVDRLATSGAILTAAVEDTMRTVPRHLFVPDAAPGEAYAEQA
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCEEECCCCCCCHHHHHHH
VITKRAPDGTSLSYASGPGIVAMMLEQLIVLPGQRILEIGTGTGYNAALLAHLAGPGGHV
HEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCEE
TTIDIDPDITSAATSALAAAGFEKVTVLTGDGTFGDPDSHVHDRLIATVGVWDISSAWWD
EEEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHEEEEEECCHHHHH
QLAPGGRLVLPLHWRGQTRAVTFRHDGNRMISESVELCGSVPMIGQAGERTAAIHHDGLV
HCCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEE
ALHWDQDQAIDPGTLTGVLDRRRTIALSGVEVGPYDPFDGIWLRLTATEPGCCRIEATPE
EEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCHH
AVKSALCAPAIPQRSPALVDRDSLAYLSLARNHTGPGRHTLSVISHGPNRHNLADRLIEG
HHHHHHCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHH
IHTWNSDRTATPTVTAHHGPAGLAHQPPGLIRKPDSPLTITF
HHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]