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The map label for this gene is ramA [H]

Identifier: 158318398

GI number: 158318398

Start: 8092844

End: 8093845

Strand: Direct

Name: ramA [H]

Synonym: Franean1_6663

Alternate gene names: 158318398

Gene position: 8092844-8093845 (Clockwise)

Preceding gene: 158318397

Following gene: 158318399

Centisome position: 90.1

GC content: 74.55

Gene sequence:

>1002_bases
GTGAGCGCGGACCGCGGGGACGGCGCGCGTGGGGGGCCGGAGCCGGCCTCCCACGCGGTGACGATCGCCGCGGTGACCGC
GCCGTTCACCCGTGACCTGGACGAATGCCTCGCGGCGATCAGTCGGCTGGTCGACGGGGCCCGGCGGCGCGGGGTGGATC
TCCTCGTCCTGCCCGAGGGGGCGCTCGGCGGCTACCTGCGGGCGCTGCCCCCGCGCGGGGACGATCTGGCCCCGCGCGGG
GGGCCGCCCGCCCTCGATCCGGACGGTCCGGAGATCACCCGGCTGGCGGCGATCGCCGGGGATATGGTGGTCTGCGCGGG
ATACGCGGAGCGTGACGGGCGGTACCGGTACAACAGTGCGGTGTGTGTGCACGGTGATGGCGTCCTCGGCCGGCATCGCA
AGGTCCACCAGCCGCTCGGCGAGTCCCTCGCCTACGAGGCCGGCCGTTCCTTCACCGCGTTCGACAGCCCACTCGGCCGG
ATGGGGATGATGATCTGTTACGACAAGGCGTTCCCCGAGTCCGGGCGTAGCCTGGCGCTCGCTGGCGCGGACATCATCGC
CTGCCTGTCGGCCTGGCCGGCCTCGCGTACCCACGCGGCCGACGACATCGCCGCGGACAGGTGGCGGCACCGCTTCGACC
TCTACGACCAGGTGCGCGCATTGGAGAACCAGGTCGTGTGGGTCTCGTCCAACCAGGCCGGCACGTTCGGCTCGCTGCGC
TTCGTCGGCAACGCGAAGATCGTCCATCCGGACGGCTCCGTGCTCGCCTCGACCGGCACGGGCGCGGGGATGGCCGTCGC
GACGGTCGACGTCACCGCGGCGCTACGGGCGGCCCGCAGCGGCCTGAACCACCTGCGGGACCGCCGCGGCGCGAGCTACG
AGAAGCAGTGCCTGCTCGCCGGTAAGCCCTACGACCCGCGGCGGGCCGCCCGCCCGGCGGCCCCCCGGCCCACGGCCGAG
CACGGATCTCGCCCGTCCCGGCCCGCGGGACCGGCGCACTGA

Upstream 100 bases:

>100_bases
GCTGCGGCCGGCTACTCAACGGGACGGGAGTGACCCTGCGCTGGCCGGACGGTGTGCTGACCCCGGCGCTGTCCGGCCAT
GTGACGGGGCTGGGCCACGC

Downstream 100 bases:

>100_bases
TGCCCGACGCGATCATCATCGGCGCGGGGCCGGCCGGCCTGACCGCGGGCCTGCTGCTCGCGCGCCGCGGCTGGCGGGTC
GACGTGCTCGAACGGGACGT

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSADRGDGARGGPEPASHAVTIAAVTAPFTRDLDECLAAISRLVDGARRRGVDLLVLPEGALGGYLRALPPRGDDLAPRG
GPPALDPDGPEITRLAAIAGDMVVCAGYAERDGRYRYNSAVCVHGDGVLGRHRKVHQPLGESLAYEAGRSFTAFDSPLGR
MGMMICYDKAFPESGRSLALAGADIIACLSAWPASRTHAADDIAADRWRHRFDLYDQVRALENQVVWVSSNQAGTFGSLR
FVGNAKIVHPDGSVLASTGTGAGMAVATVDVTAALRAARSGLNHLRDRRGASYEKQCLLAGKPYDPRRAARPAAPRPTAE
HGSRPSRPAGPAH

Sequences:

>Translated_333_residues
MSADRGDGARGGPEPASHAVTIAAVTAPFTRDLDECLAAISRLVDGARRRGVDLLVLPEGALGGYLRALPPRGDDLAPRG
GPPALDPDGPEITRLAAIAGDMVVCAGYAERDGRYRYNSAVCVHGDGVLGRHRKVHQPLGESLAYEAGRSFTAFDSPLGR
MGMMICYDKAFPESGRSLALAGADIIACLSAWPASRTHAADDIAADRWRHRFDLYDQVRALENQVVWVSSNQAGTFGSLR
FVGNAKIVHPDGSVLASTGTGAGMAVATVDVTAALRAARSGLNHLRDRRGASYEKQCLLAGKPYDPRRAARPAAPRPTAE
HGSRPSRPAGPAH
>Mature_332_residues
SADRGDGARGGPEPASHAVTIAAVTAPFTRDLDECLAAISRLVDGARRRGVDLLVLPEGALGGYLRALPPRGDDLAPRGG
PPALDPDGPEITRLAAIAGDMVVCAGYAERDGRYRYNSAVCVHGDGVLGRHRKVHQPLGESLAYEAGRSFTAFDSPLGRM
GMMICYDKAFPESGRSLALAGADIIACLSAWPASRTHAADDIAADRWRHRFDLYDQVRALENQVVWVSSNQAGTFGSLRF
VGNAKIVHPDGSVLASTGTGAGMAVATVDVTAALRAARSGLNHLRDRRGASYEKQCLLAGKPYDPRRAARPAAPRPTAEH
GSRPSRPAGPAH

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=291, Percent_Identity=28.8659793814433, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI297632350, Length=291, Percent_Identity=28.8659793814433, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI297632348, Length=291, Percent_Identity=28.8659793814433, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 35129; Mature: 34998

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADRGDGARGGPEPASHAVTIAAVTAPFTRDLDECLAAISRLVDGARRRGVDLLVLPEG
CCCCCCCCCCCCCCCCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
ALGGYLRALPPRGDDLAPRGGPPALDPDGPEITRLAAIAGDMVVCAGYAERDGRYRYNSA
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEECCE
VCVHGDGVLGRHRKVHQPLGESLAYEAGRSFTAFDSPLGRMGMMICYDKAFPESGRSLAL
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEHHCCCHHHHCEEEEEECCCCCCCCEEEE
AGADIIACLSAWPASRTHAADDIAADRWRHRFDLYDQVRALENQVVWVSSNQAGTFGSLR
EHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
FVGNAKIVHPDGSVLASTGTGAGMAVATVDVTAALRAARSGLNHLRDRRGASYEKQCLLA
EECCEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
GKPYDPRRAARPAAPRPTAEHGSRPSRPAGPAH
CCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SADRGDGARGGPEPASHAVTIAAVTAPFTRDLDECLAAISRLVDGARRRGVDLLVLPEG
CCCCCCCCCCCCCCCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCC
ALGGYLRALPPRGDDLAPRGGPPALDPDGPEITRLAAIAGDMVVCAGYAERDGRYRYNSA
CCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCEEECCE
VCVHGDGVLGRHRKVHQPLGESLAYEAGRSFTAFDSPLGRMGMMICYDKAFPESGRSLAL
EEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEHHCCCHHHHCEEEEEECCCCCCCCEEEE
AGADIIACLSAWPASRTHAADDIAADRWRHRFDLYDQVRALENQVVWVSSNQAGTFGSLR
EHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
FVGNAKIVHPDGSVLASTGTGAGMAVATVDVTAALRAARSGLNHLRDRRGASYEKQCLLA
EECCEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
GKPYDPRRAARPAAPRPTAEHGSRPSRPAGPAH
CCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA