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The map label for this gene is amt [H]

Identifier: 158317945

GI number: 158317945

Start: 7537420

End: 7538703

Strand: Reverse

Name: amt [H]

Synonym: Franean1_6204

Alternate gene names: 158317945

Gene position: 7538703-7537420 (Counterclockwise)

Preceding gene: 158317947

Following gene: 158317944

Centisome position: 83.93

GC content: 67.13

Gene sequence:

>1284_bases
ATGGACGAATTGAGCTCGGGCGATACCGCGTGGGTGCTCGTCAGCGCCGCACTTGTGCTCTTCATGACGCCCGGCCTGGC
CTTCTTCTACGGCGGAATGGTCCGTGCGAAGAATGTGCTCGGCATGCTCATGCAGAACTTCTTCTGCATGGGGATGATCA
CACTGGTGTGGTCGGTCGTCGGTTTCAGTATCGCCTTCGGTGAGGGGAACGGATTCTGGGGCGGTTTCGACTTCTTCGCT
CTCCAGGATCTCACTGCGAGTGACGGGGCGATCCCGTACGTCGCCTTCGTCGCCTTCCAGATGATGTTCGCGGTCATCAC
GCCCGCCCTGATCACCGGTTCCACCGCGGACCGGATGAAGTTCAGCTCCTTCGCGATCTTCGTCACGCTGTGGTCGGTCC
TGGTCTACTCGCCCATCGCGCACTGGGTGTGGGCGAGCAGCGGCTGGCTGTTCAAGCGCGGCGCGCTCGACTTCGCGGGC
GGCACCGTCGTCCACGCGAACGCCGGTATCGCCGCGATCGTGCTGGCGCTGTTCCTCGGCAAGCGGCGCGGCTGGCCGAC
CGGCGACTTCCGCCCGCACAACGTGCCGTTTGTGCTGCTGGGCACCGGCATCCTGTGGGTGGGCTGGTTCGGGTTCAACG
CCGGCTCGGCGCTGGGTGCGAACAAGGTCGCCGCGATCGCGCTGCTCAACACGCAGATCGGTGCGGCCACGGCCATCCTC
GGCTGGATCGCGATCGAGCGGCTGCGGGACGGCAAGGCCACCACGCTCGGCGCCGCGTCGGGTGCCGTCGCGGGTCTGGT
CGCGATCACCCCGGCCTGTGGGTTCGTCTCGCCGATGGGCTCGATCGCCATCGGCCTGCTGGCGGGTGCGATCTGCGCGC
TGGCAACGTCCATCAAGGGCAAGGTCGGCATCGACGACTCGCTCGACGTCGGCGCCGTCCACCTGGTCGGCGGTGTCCTC
GGTGCGCTGCTGACCGGCTTCTTCGCCACCCTCGACACCAATGAGGCCGGCAAGGACGGTGTGTTCTACGGCGGTCCGTG
GAGCGTCGTGGGTGAGCAGGCGCTGGCCATCGGCGCGACGCTGGGCTACTCGGCGGTCGTGACCGCCCTGATCGCCCTCG
CGCTCAAGTACACGATCGGGCTGAAGGTCTCGGACGAGGATGAGGAGATCGGCCTCGACGAGGCGCTGCACGGTGAGACG
GCCTACCGGCTGACGTCCATCGGCGGCAGCGGGTTCACCTCCGCCGTCACCACGACCAAGGTGCCTGAGGAGGCCAAGGC
ATGA

Upstream 100 bases:

>100_bases
TATCGACTCTTCGGCCGAAGGACCGGGACGAGCGCAGCGTGGCCGGGGACCGGGGTAGCGCAGTCCGCGCGGTGCGCGCG
GGGGGAATGACAGGAGAGAC

Downstream 100 bases:

>100_bases
AGCTCGTGACGGCAGTCATCAAGCCGTTCAAGGTGGACGACGTCAAGACCGCCCTCGAGGGCATCGGCGTCCACGGGCTG
ACGATCTCCGAGGTGCAGGG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MDELSSGDTAWVLVSAALVLFMTPGLAFFYGGMVRAKNVLGMLMQNFFCMGMITLVWSVVGFSIAFGEGNGFWGGFDFFA
LQDLTASDGAIPYVAFVAFQMMFAVITPALITGSTADRMKFSSFAIFVTLWSVLVYSPIAHWVWASSGWLFKRGALDFAG
GTVVHANAGIAAIVLALFLGKRRGWPTGDFRPHNVPFVLLGTGILWVGWFGFNAGSALGANKVAAIALLNTQIGAATAIL
GWIAIERLRDGKATTLGAASGAVAGLVAITPACGFVSPMGSIAIGLLAGAICALATSIKGKVGIDDSLDVGAVHLVGGVL
GALLTGFFATLDTNEAGKDGVFYGGPWSVVGEQALAIGATLGYSAVVTALIALALKYTIGLKVSDEDEEIGLDEALHGET
AYRLTSIGGSGFTSAVTTTKVPEEAKA

Sequences:

>Translated_427_residues
MDELSSGDTAWVLVSAALVLFMTPGLAFFYGGMVRAKNVLGMLMQNFFCMGMITLVWSVVGFSIAFGEGNGFWGGFDFFA
LQDLTASDGAIPYVAFVAFQMMFAVITPALITGSTADRMKFSSFAIFVTLWSVLVYSPIAHWVWASSGWLFKRGALDFAG
GTVVHANAGIAAIVLALFLGKRRGWPTGDFRPHNVPFVLLGTGILWVGWFGFNAGSALGANKVAAIALLNTQIGAATAIL
GWIAIERLRDGKATTLGAASGAVAGLVAITPACGFVSPMGSIAIGLLAGAICALATSIKGKVGIDDSLDVGAVHLVGGVL
GALLTGFFATLDTNEAGKDGVFYGGPWSVVGEQALAIGATLGYSAVVTALIALALKYTIGLKVSDEDEEIGLDEALHGET
AYRLTSIGGSGFTSAVTTTKVPEEAKA
>Mature_427_residues
MDELSSGDTAWVLVSAALVLFMTPGLAFFYGGMVRAKNVLGMLMQNFFCMGMITLVWSVVGFSIAFGEGNGFWGGFDFFA
LQDLTASDGAIPYVAFVAFQMMFAVITPALITGSTADRMKFSSFAIFVTLWSVLVYSPIAHWVWASSGWLFKRGALDFAG
GTVVHANAGIAAIVLALFLGKRRGWPTGDFRPHNVPFVLLGTGILWVGWFGFNAGSALGANKVAAIALLNTQIGAATAIL
GWIAIERLRDGKATTLGAASGAVAGLVAITPACGFVSPMGSIAIGLLAGAICALATSIKGKVGIDDSLDVGAVHLVGGVL
GALLTGFFATLDTNEAGKDGVFYGGPWSVVGEQALAIGATLGYSAVVTALIALALKYTIGLKVSDEDEEIGLDEALHGET
AYRLTSIGGSGFTSAVTTTKVPEEAKA

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Homo sapiens, GI224548945, Length=201, Percent_Identity=31.8407960199005, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786656, Length=403, Percent_Identity=48.1389578163772, Blast_Score=318, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17550182, Length=448, Percent_Identity=31.6964285714286, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=30.0230946882217, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=28.9044289044289, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI133931100, Length=422, Percent_Identity=26.7772511848341, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=430, Percent_Identity=36.5116279069767, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324187, Length=441, Percent_Identity=36.9614512471655, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6321559, Length=420, Percent_Identity=35.2380952380952, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI161078314, Length=437, Percent_Identity=30.4347826086957, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44155; Mature: 44155

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELSSGDTAWVLVSAALVLFMTPGLAFFYGGMVRAKNVLGMLMQNFFCMGMITLVWSVV
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFSIAFGEGNGFWGGFDFFALQDLTASDGAIPYVAFVAFQMMFAVITPALITGSTADRMK
HHEEEEECCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FSSFAIFVTLWSVLVYSPIAHWVWASSGWLFKRGALDFAGGTVVHANAGIAAIVLALFLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHH
KRRGWPTGDFRPHNVPFVLLGTGILWVGWFGFNAGSALGANKVAAIALLNTQIGAATAIL
CCCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHH
GWIAIERLRDGKATTLGAASGAVAGLVAITPACGFVSPMGSIAIGLLAGAICALATSIKG
HHHHHHHHCCCCCEEECCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCC
KVGIDDSLDVGAVHLVGGVLGALLTGFFATLDTNEAGKDGVFYGGPWSVVGEQALAIGAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHCCHHHHHHHH
LGYSAVVTALIALALKYTIGLKVSDEDEEIGLDEALHGETAYRLTSIGGSGFTSAVTTTK
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHCCCHHEEEEECCCCCCCCCCEECC
VPEEAKA
CCHHCCC
>Mature Secondary Structure
MDELSSGDTAWVLVSAALVLFMTPGLAFFYGGMVRAKNVLGMLMQNFFCMGMITLVWSVV
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFSIAFGEGNGFWGGFDFFALQDLTASDGAIPYVAFVAFQMMFAVITPALITGSTADRMK
HHEEEEECCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FSSFAIFVTLWSVLVYSPIAHWVWASSGWLFKRGALDFAGGTVVHANAGIAAIVLALFLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHH
KRRGWPTGDFRPHNVPFVLLGTGILWVGWFGFNAGSALGANKVAAIALLNTQIGAATAIL
CCCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHH
GWIAIERLRDGKATTLGAASGAVAGLVAITPACGFVSPMGSIAIGLLAGAICALATSIKG
HHHHHHHHCCCCCEEECCCCHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCC
KVGIDDSLDVGAVHLVGGVLGALLTGFFATLDTNEAGKDGVFYGGPWSVVGEQALAIGAT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHCCHHHHHHHH
LGYSAVVTALIALALKYTIGLKVSDEDEEIGLDEALHGETAYRLTSIGGSGFTSAVTTTK
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHCCCHHEEEEECCCCCCCCCCEECC
VPEEAKA
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]