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The map label for this gene is pucA [H]

Identifier: 158317938

GI number: 158317938

Start: 7528409

End: 7529578

Strand: Reverse

Name: pucA [H]

Synonym: Franean1_6196

Alternate gene names: 158317938

Gene position: 7529578-7528409 (Counterclockwise)

Preceding gene: 158317939

Following gene: 158317937

Centisome position: 83.83

GC content: 74.1

Gene sequence:

>1170_bases
ATGCGTGACGTGCTCCCCGACCTCGAGAGCTGGTGGCAGACGGGGGAGACCGTCGGCCTGGCGACGGTCGTCGGCACCTG
GAAGTCGGCCCCGCGCCAGCCGGGTGCGGCGATGGCTGTCACCCCGGACGGCTCGGTCGTGGGCAGTGTCTCGGGCGGCT
GCGTCGAGGGGGCCGTGTACGACCTGGCCGAGCAGTGCCGCGCGACGTCCGTCCCCGTCCTGCAGCGCTACGGGGTCAGT
GACGACGACGCGTTCGCGGTGGGCCTCACCTGCGGGGGCATCATCGACGTCTTCGTCGAGGCGGTGAGCCGGCGGACGTT
CCCCGAGCTCGGCGGGATCCTCGCCGACGTCCGGGACGGCCGGCCGGTCGCGGTCGCGACGCTGGTCAGCGGGGTGGGCA
CCAGCCCCGTCGGGTCCCGCCTAGTCGTCCGGCCGGGCTCCGTCGAGGGCACCCTCGGCAGCGCGCGGTTCGACACGGCC
GTCCGCGACGACGCGCGCGGGATGCTGGAGCAGGGCCGGACGGGCATCGTCCGCTACGGCCCGGACGGCGAGCGGCGCGG
TGACGACAGTGCCGTCTTTGTCGCCTCCTACGCGCCGCGGCCGCGGCTGATCGTCTTCGGTGCCATCGACTTCGCCGCCG
CGCTGGCCAGGGTGGGCGCCTTCCTCGGCTACCGGGTGACCGTGTGCGACGCACGGGCGACGTTCGCCACCAGCAAGCGG
TTCCCCGAGGCCGACGAGGTGGTCGTCGACTGGCCGCACCGCTACCTCGCCGCGACGGCGGTGGACGAGCGCACGACCGT
CTGCGTCCTCACCCACGATCCGAAGTTCGACGTGCCGCTCCTGGAGGTCGCGCTGCGCCGGCCGCTGGCCTACCTCGGCG
TCATGGGCTCGCGCCGCACCAACGCCGACCGCGCGCGGCGGCTGCGGGAGCTCGGCTTCTCCGACGCCGACCTGGCCCGC
CTGCACGCGCCCATCGGCCTGGACATCGGGGGGCGCACACCGGAGGAGACCGCGATCTCCATCGCCGCGGAGATCATCGC
GACGCGGCACGGGGGATCGGGCGCCCAGCTACGGCAGCTGTCCGGCCCCATCCACCATGACCTGCCCGCGCCGTTCGGTA
ACCCATCCGCTTCGTTCGGTGACGTTCGGGCGTCGGCCCATGACGGCTGA

Upstream 100 bases:

>100_bases
CCGACCGCCGCCGGCATGTCCGCGGCGCTTCCCCACGTGGACGACCTGTTGGCCGGGCATACGTTCCAGGCACTGCGAGG
ACTTGCCGAGGTGATCTCCG

Downstream 100 bases:

>100_bases
ACGTCGAGTGCCGATCGCACGGATCTGATTCCTGTTACTCTCCGGCCGTGCTGTTCGCCTTACGCTTCGACTTCCGTAAT
CCGGATCTCGCCGGTACGAC

Product: hypothetical protein

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MRDVLPDLESWWQTGETVGLATVVGTWKSAPRQPGAAMAVTPDGSVVGSVSGGCVEGAVYDLAEQCRATSVPVLQRYGVS
DDDAFAVGLTCGGIIDVFVEAVSRRTFPELGGILADVRDGRPVAVATLVSGVGTSPVGSRLVVRPGSVEGTLGSARFDTA
VRDDARGMLEQGRTGIVRYGPDGERRGDDSAVFVASYAPRPRLIVFGAIDFAAALARVGAFLGYRVTVCDARATFATSKR
FPEADEVVVDWPHRYLAATAVDERTTVCVLTHDPKFDVPLLEVALRRPLAYLGVMGSRRTNADRARRLRELGFSDADLAR
LHAPIGLDIGGRTPEETAISIAAEIIATRHGGSGAQLRQLSGPIHHDLPAPFGNPSASFGDVRASAHDG

Sequences:

>Translated_389_residues
MRDVLPDLESWWQTGETVGLATVVGTWKSAPRQPGAAMAVTPDGSVVGSVSGGCVEGAVYDLAEQCRATSVPVLQRYGVS
DDDAFAVGLTCGGIIDVFVEAVSRRTFPELGGILADVRDGRPVAVATLVSGVGTSPVGSRLVVRPGSVEGTLGSARFDTA
VRDDARGMLEQGRTGIVRYGPDGERRGDDSAVFVASYAPRPRLIVFGAIDFAAALARVGAFLGYRVTVCDARATFATSKR
FPEADEVVVDWPHRYLAATAVDERTTVCVLTHDPKFDVPLLEVALRRPLAYLGVMGSRRTNADRARRLRELGFSDADLAR
LHAPIGLDIGGRTPEETAISIAAEIIATRHGGSGAQLRQLSGPIHHDLPAPFGNPSASFGDVRASAHDG
>Mature_389_residues
MRDVLPDLESWWQTGETVGLATVVGTWKSAPRQPGAAMAVTPDGSVVGSVSGGCVEGAVYDLAEQCRATSVPVLQRYGVS
DDDAFAVGLTCGGIIDVFVEAVSRRTFPELGGILADVRDGRPVAVATLVSGVGTSPVGSRLVVRPGSVEGTLGSARFDTA
VRDDARGMLEQGRTGIVRYGPDGERRGDDSAVFVASYAPRPRLIVFGAIDFAAALARVGAFLGYRVTVCDARATFATSKR
FPEADEVVVDWPHRYLAATAVDERTTVCVLTHDPKFDVPLLEVALRRPLAYLGVMGSRRTNADRARRLRELGFSDADLAR
LHAPIGLDIGGRTPEETAISIAAEIIATRHGGSGAQLRQLSGPIHHDLPAPFGNPSASFGDVRASAHDG

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789240, Length=258, Percent_Identity=32.1705426356589, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786477, Length=336, Percent_Identity=27.3809523809524, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 41030; Mature: 41030

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDVLPDLESWWQTGETVGLATVVGTWKSAPRQPGAAMAVTPDGSVVGSVSGGCVEGAVY
CCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHH
DLAEQCRATSVPVLQRYGVSDDDAFAVGLTCGGIIDVFVEAVSRRTFPELGGILADVRDG
HHHHHHCCCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
RPVAVATLVSGVGTSPVGSRLVVRPGSVEGTLGSARFDTAVRDDARGMLEQGRTGIVRYG
CCEEHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
PDGERRGDDSAVFVASYAPRPRLIVFGAIDFAAALARVGAFLGYRVTVCDARATFATSKR
CCCCCCCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCC
FPEADEVVVDWPHRYLAATAVDERTTVCVLTHDPKFDVPLLEVALRRPLAYLGVMGSRRT
CCCCCCEEEECCCHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHCCCCCCC
NADRARRLRELGFSDADLARLHAPIGLDIGGRTPEETAISIAAEIIATRHGGSGAQLRQL
CHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
SGPIHHDLPAPFGNPSASFGDVRASAHDG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRDVLPDLESWWQTGETVGLATVVGTWKSAPRQPGAAMAVTPDGSVVGSVSGGCVEGAVY
CCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHH
DLAEQCRATSVPVLQRYGVSDDDAFAVGLTCGGIIDVFVEAVSRRTFPELGGILADVRDG
HHHHHHCCCCCCHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
RPVAVATLVSGVGTSPVGSRLVVRPGSVEGTLGSARFDTAVRDDARGMLEQGRTGIVRYG
CCEEHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEC
PDGERRGDDSAVFVASYAPRPRLIVFGAIDFAAALARVGAFLGYRVTVCDARATFATSKR
CCCCCCCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEECCHHHHHHCCC
FPEADEVVVDWPHRYLAATAVDERTTVCVLTHDPKFDVPLLEVALRRPLAYLGVMGSRRT
CCCCCCEEEECCCHHHHHHEECCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHCCCCCCC
NADRARRLRELGFSDADLARLHAPIGLDIGGRTPEETAISIAAEIIATRHGGSGAQLRQL
CHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
SGPIHHDLPAPFGNPSASFGDVRASAHDG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]