Click here to switch to the map view.

The map label for this gene is cofH2 [H]

Identifier: 158317841

GI number: 158317841

Start: 7424410

End: 7425957

Strand: Reverse

Name: cofH2 [H]

Synonym: Franean1_6099

Alternate gene names: 158317841

Gene position: 7425957-7424410 (Counterclockwise)

Preceding gene: 158317842

Following gene: 158317840

Centisome position: 82.68

GC content: 73.0

Gene sequence:

>1548_bases
ATGACGCCCGTGTCGGGCCGGACAACATGCACTCCGACGTGCACCGTTCCGACTTCGGCGGCCAGAACGGCGCCGGGCCT
TCCGGCCCGGGAACGACCGGTGCGGGCGGGCCCGGCGCCGGGGCGGGTTCCGGCTCCCGCGACAGCTCGGGATACGACCG
GATCTGAGCCGGTCCGCCGCCCGGGCCGGCGCCCGCCGGAGCCGGCGCCACCCGGGCCGGCGCTTCCCCGGGCTGGTGCA
GCCCGGGCCGGGGCTTCTGAGGCGGTGTCTTCCGAGCCGGCTCGTCCGAGTTCGGCCGCCCGCCGCCCGGGGCTCGCCCG
GCGCAGGTCGTACGATCCTGGGGTGAGCCTTGTTGATGCCGACGGTGAGTCCCCGGCGGGCCGGGCCCGCGTTCCCGACG
CCGAGATCCGCGCGCTGCTCGACCGGGCGGCCGACGGCGGGCGGATCTCGCCGGAGGAGGCGCTCCTCCTCTACACGTCC
GCTCCGCTGCACGGGCTCGGGCGCGCGGCCGACGCCGTCCGTCGCCGCCGGTACCCGGACGGCATCGCCACCTACATCAT
CGACCGGAACATCAACTACACCAACGTCTGCGTGACGGCCTGCCGGTTCTGCGCCTTCTACCGGTCGCCGAAGCACGCCG
AGGGCTGGGTCCGCGACCTCGACGACATCGTTGCCAAGTGCGGCGAGGCGGTCGAGCTGGGCGCCACCCAGATCATGCTG
CAGGGCGGCCACAACCCCGAGTTCGGGATCGAGTGGTACGAGCGGACGTTCGCCGGCATCAAGGCCGCCTACCCGCAGCT
CGCCCTGCACTCGCTCGGCGCCAGCGAGGTCGTGCACATCGCGCGGACGTCCGACCTGGACTTCGCCGAGGTGATCACCC
GGCTGCGCGACGCCGGGCTCGACAGCTTCGCCGGCGCGGGCGCGGAGATCCTCACCGAGCGTCCCCGGCACGCGATCGCC
CCGCTCAAGGAGCCCGGCCACGTGTGGCTGTCGGTCATGGAGATCGCCCACGGCCTGGGCCTGGAGTCGACGGCGACGTT
CATGATGGGCACCGGCGAGACCAACGCCGAGCGCATCGAGCACATGCGCATGATCCGGGACGTCCAGGACCGCACCGGCG
GGTTCCGCTCGTTCATCCCGTGGACGTACCAGCCGGAGAACAACCACCTCGGCGGCCGGACGCAGGCGACGACCCTGGAG
TACCTGCGGCTGGTGGCCGTCGCGCGGCTGTTCTTCGACAACGTGGCCCACCTGCAGGGCTCGTGGCTGACCACCGGCAA
GGAGGTCGGCCAGCTCACCCTGCACATGGGCGCCGACGATCTCGGCTCGGTGATGCTCGAGGAGAACGTCGTCTCCTCCG
CGGGGGCCCGGCACCGCACCAACCGGTCCGAGCTCATCCACCTGATCCGGGCCGCCGACCGCATCCCGGCGCAGCGCGAC
ACGCTGTACCGGCACCTCGTCGTGCACCGTGACCCGGCGCTCGACCCGGTCGACGACCGGGTGGCCTCGCACTTCTCCTC
GACCGCGCTGCCGCTGGTGTCGACCTGA

Upstream 100 bases:

>100_bases
AAGGCGCGGGCCGCGGCGAACAAGCCGGAGAATCGCGACAAGATCCGACAGCTGTCCAGCAAGCTCACCAGCCGCGGCCG
TAGCGGGCAGGGTACCGGCC

Downstream 100 bases:

>100_bases
GCCACGACCAGCGCCACGAACCGAACGCTGTCCCGAAGGCCGTCCCGAACGCTGTGTCGATGTCCGCTGAGGCGGTCAGG
GGTGAGCCGGCCGCGGTGGG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 2 [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTPVSGRTTCTPTCTVPTSAARTAPGLPARERPVRAGPAPGRVPAPATARDTTGSEPVRRPGRRPPEPAPPGPALPRAGA
ARAGASEAVSSEPARPSSAARRPGLARRRSYDPGVSLVDADGESPAGRARVPDAEIRALLDRAADGGRISPEEALLLYTS
APLHGLGRAADAVRRRRYPDGIATYIIDRNINYTNVCVTACRFCAFYRSPKHAEGWVRDLDDIVAKCGEAVELGATQIML
QGGHNPEFGIEWYERTFAGIKAAYPQLALHSLGASEVVHIARTSDLDFAEVITRLRDAGLDSFAGAGAEILTERPRHAIA
PLKEPGHVWLSVMEIAHGLGLESTATFMMGTGETNAERIEHMRMIRDVQDRTGGFRSFIPWTYQPENNHLGGRTQATTLE
YLRLVAVARLFFDNVAHLQGSWLTTGKEVGQLTLHMGADDLGSVMLEENVVSSAGARHRTNRSELIHLIRAADRIPAQRD
TLYRHLVVHRDPALDPVDDRVASHFSSTALPLVST

Sequences:

>Translated_515_residues
MTPVSGRTTCTPTCTVPTSAARTAPGLPARERPVRAGPAPGRVPAPATARDTTGSEPVRRPGRRPPEPAPPGPALPRAGA
ARAGASEAVSSEPARPSSAARRPGLARRRSYDPGVSLVDADGESPAGRARVPDAEIRALLDRAADGGRISPEEALLLYTS
APLHGLGRAADAVRRRRYPDGIATYIIDRNINYTNVCVTACRFCAFYRSPKHAEGWVRDLDDIVAKCGEAVELGATQIML
QGGHNPEFGIEWYERTFAGIKAAYPQLALHSLGASEVVHIARTSDLDFAEVITRLRDAGLDSFAGAGAEILTERPRHAIA
PLKEPGHVWLSVMEIAHGLGLESTATFMMGTGETNAERIEHMRMIRDVQDRTGGFRSFIPWTYQPENNHLGGRTQATTLE
YLRLVAVARLFFDNVAHLQGSWLTTGKEVGQLTLHMGADDLGSVMLEENVVSSAGARHRTNRSELIHLIRAADRIPAQRD
TLYRHLVVHRDPALDPVDDRVASHFSSTALPLVST
>Mature_514_residues
TPVSGRTTCTPTCTVPTSAARTAPGLPARERPVRAGPAPGRVPAPATARDTTGSEPVRRPGRRPPEPAPPGPALPRAGAA
RAGASEAVSSEPARPSSAARRPGLARRRSYDPGVSLVDADGESPAGRARVPDAEIRALLDRAADGGRISPEEALLLYTSA
PLHGLGRAADAVRRRRYPDGIATYIIDRNINYTNVCVTACRFCAFYRSPKHAEGWVRDLDDIVAKCGEAVELGATQIMLQ
GGHNPEFGIEWYERTFAGIKAAYPQLALHSLGASEVVHIARTSDLDFAEVITRLRDAGLDSFAGAGAEILTERPRHAIAP
LKEPGHVWLSVMEIAHGLGLESTATFMMGTGETNAERIEHMRMIRDVQDRTGGFRSFIPWTYQPENNHLGGRTQATTLEY
LRLVAVARLFFDNVAHLQGSWLTTGKEVGQLTLHMGADDLGSVMLEENVVSSAGARHRTNRSELIHLIRAADRIPAQRDT
LYRHLVVHRDPALDPVDDRVASHFSSTALPLVST

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 55613; Mature: 55482

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVSGRTTCTPTCTVPTSAARTAPGLPARERPVRAGPAPGRVPAPATARDTTGSEPVRR
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHC
PGRRPPEPAPPGPALPRAGAARAGASEAVSSEPARPSSAARRPGLARRRSYDPGVSLVDA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCHHHCCCCCCCEEEEC
DGESPAGRARVPDAEIRALLDRAADGGRISPEEALLLYTSAPLHGLGRAADAVRRRRYPD
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
GIATYIIDRNINYTNVCVTACRFCAFYRSPKHAEGWVRDLDDIVAKCGEAVELGATQIML
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEE
QGGHNPEFGIEWYERTFAGIKAAYPQLALHSLGASEVVHIARTSDLDFAEVITRLRDAGL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCHHHHHHHHHHCCC
DSFAGAGAEILTERPRHAIAPLKEPGHVWLSVMEIAHGLGLESTATFMMGTGETNAERIE
HHHHCCCHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHH
HMRMIRDVQDRTGGFRSFIPWTYQPENNHLGGRTQATTLEYLRLVAVARLFFDNVAHLQG
HHHHHHHHHHHCCCHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SWLTTGKEVGQLTLHMGADDLGSVMLEENVVSSAGARHRTNRSELIHLIRAADRIPAQRD
CCCCCCHHHCEEEEEECCCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHH
TLYRHLVVHRDPALDPVDDRVASHFSSTALPLVST
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TPVSGRTTCTPTCTVPTSAARTAPGLPARERPVRAGPAPGRVPAPATARDTTGSEPVRR
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHC
PGRRPPEPAPPGPALPRAGAARAGASEAVSSEPARPSSAARRPGLARRRSYDPGVSLVDA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCCCHHHCCCCCCCEEEEC
DGESPAGRARVPDAEIRALLDRAADGGRISPEEALLLYTSAPLHGLGRAADAVRRRRYPD
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
GIATYIIDRNINYTNVCVTACRFCAFYRSPKHAEGWVRDLDDIVAKCGEAVELGATQIML
CCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEE
QGGHNPEFGIEWYERTFAGIKAAYPQLALHSLGASEVVHIARTSDLDFAEVITRLRDAGL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCHHHHHHHHHHCCC
DSFAGAGAEILTERPRHAIAPLKEPGHVWLSVMEIAHGLGLESTATFMMGTGETNAERIE
HHHHCCCHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHH
HMRMIRDVQDRTGGFRSFIPWTYQPENNHLGGRTQATTLEYLRLVAVARLFFDNVAHLQG
HHHHHHHHHHHCCCHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
SWLTTGKEVGQLTLHMGADDLGSVMLEENVVSSAGARHRTNRSELIHLIRAADRIPAQRD
CCCCCCHHHCEEEEEECCCHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHH
TLYRHLVVHRDPALDPVDDRVASHFSSTALPLVST
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]