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The map label for this gene is pcrA [H]

Identifier: 158317729

GI number: 158317729

Start: 7295139

End: 7297694

Strand: Reverse

Name: pcrA [H]

Synonym: Franean1_5987

Alternate gene names: 158317729

Gene position: 7297694-7295139 (Counterclockwise)

Preceding gene: 158317730

Following gene: 158317728

Centisome position: 81.25

GC content: 72.03

Gene sequence:

>2556_bases
ATGAGCACTCTTTTCGACGACACCTTCGGCCCCGGGGCCCTGGTCGACGGCGGTGCCCCGGCCGGCTCCGGCGACGGCCA
CGAGCCGCACGGTGATCTTCCTGGCGGCGGTCGGGCGTCCCGTGGGGCCTTCGGCCCGGCGGTGCTCGAGCCGGGCGGCG
CCACCCGCGCCCGGGCGCCCAGGCTCGACCCGGACGAGCTGCTCGAGGGGCTCAACCCGCAGCAGCGTGCCGCGGTGGTG
CATGCCGGCTCGCCGTTGCTGGTCGTCGCGGGTGCCGGGTCCGGCAAGACCCGCGTGCTCACGCACCGGATCGCGTACCT
GCTCGCGGCGCGCGGCGCGCGGCCCGGCGAGATCCTCGCGATCACCTTCACCAACAAGGCCGCCGGCGAGATGAAGGAGC
GCGTCGAGGCGATCGTGGGCGGCCGCGCCCGGGCGATGTGGGTGAGCACCTTCCACTCCGCCTGCGTGCGCATCCTGCGC
TCGGAGGCGAGCCGGCTCGGCTTCGGCTCCTCCTTCTCGATCTACGACGCGGCGGACGCCCAGCGCCTCATCACGCTGGT
CACCAGGGATCTCGACCTCGATCCGAAGCGGCACACGGCCCGTGGGCTCGCCGCGGCGATCAGCGCGCTGAAGAACGAGC
TGGTCGACTGGGAGACGGCCCGGGACAGGGCCACGAACCACATCGAGCGCACGGTCGCCGAGGTCTACGCCGCCTACCAG
CAGCGCCTGACCCAGGCGAACGCGCTGGACTTCGACGACCTGATCATGACCACGGTCAACCTGCTGCAGGCGTTCCCGGA
CGTCGCCGAGCACTACCGGCGCCGGTTCCGCCACGTCTTGGTGGACGAGTACCAGGACACCAACCACGCCCAGTACGTGC
TCATCCGTGAGCTCGTGGGCCGCTCGGCGTCCTCGGCTGACGAGCCGGCTGCGGGCTCCGGCGAGGCTGTGTACTCCGGC
GGTTCCGCGGAGCCCGACGGGGCGGGGTGGCCCGCGAAGGCGGTGCCGCCGTCGGGTGCCGTTCCGTCCGCGGAGCTGTG
CGTCGTCGGTGACGCGGACCAGTCGATCTACGCCTTCCGTGGCGCGAACATCCGCAACATCGTCGAGTTCGAGCAGGACT
TCCCGAACGCGGCGGTCATCCTGCTGGAGCAGAACTACCGCTCCACGCAGACGATCCTCTCCGCGGCGAACGCGGTCATC
GCCCGCAACGCCCAGCGCAAGCCCAAGCGCCTGTGGTCGGACGCCGGTGACGGCGAGCGGATCGTCGGCTTCGTCGCCGA
CAACGAGCACGACGAGGCCGCGTTCGTCGCGGAGGAGATCGACCGCCTCGGCGACGAGAACCTGGCCAGGCCGGCGGACG
TCGCCGTCTTCTACCGGACGAACGCGCAGAGCCGCGTGTTCGAGGAGATCTTCATCCGGGTCGGCCTGCCGTACCGGGTC
GTCGGTGGCGTCCGGTTCTACGAGCGCCGCGAGATCCGTGATCTTCTCGCCTATCTGCGGGTGTTGGTGAACCCGACGGA
CACGGTGAACCTGCGGCGCATCCTGAACGTTCCGCGGCGCGGCATCGGTGACCGCGCGGAGGCGATGGTCTCGGCCTTCG
CCGAGCGGGAGCGGATCGCCTTCTCCGAGGCGCTGAGCCGGGTGGACGAGGTGCCCGGCGTGGCGACCAGGTCGGCCCGC
TCGATCCGAGATTTCGTCGCCCTCCTGGAGGGCCTGCGCGAGCAGCTGCCCGCCGGCCCGCTGGCCGTGATCGAGGCGAC
GCTGGAACGGACGGGCTACCTCTCGGAGCTGGTCGCGGAGGACACCATCGAGGCACAGGGCCGGGTGGAGAACCTGCGCG
AACTCGTCGGGGTCGTCCAGGAGTTCACCGAGCGGCGCCCGGACGGCACCCTCGCCGAGTTCCTGGAGCAGGTGGCGCTC
GTCGCCGATGCCGACCAGATACCCGACGACGACGGCTCGGACGGCGTGGTCACGCTGATGACCCTGCACAGCGCGAAGGG
CCTCGAGTTCCCCGTCGTGTTCCTGACCGGGCTCGAGGACGGCGTCTTCCCGCACATGCGCACGCTTGGCGACCCGACCG
GGCTGGAGGAGGAGCGCCGGCTCGCCTACGTGGGCGTCACCCGGGCCCGTGCGAGGCTGTACCTCACCAGGTCCCAGGTA
CGCAGCGCCTGGGGGCAGCCGGCCTACAACCCGCCGTCCCGGTTCCTGGGGGAGGTGCCGGACTCGCTCGTGGAGTGGCG
GCGGCTGCCCGAGCCTGCCCCGGCCGCGGGCGGTGGGCGGTTCTCCGGCGGTGGGTCGGCGGGCGCGGGCAACGGTTCCG
GTGGGTCCGGCGGGAAGCTGCCGAGCTCCCCGTTCGGGGCGAAGCCCAGCCGCCCGGCGGCGCGTCCGATCATCGAGCTG
ACCCCCGGCGACCGTGTCACCCACGACACGTTCGGTCTCGGTGTGGTCGTCACGACGAGCGGGGTGGGGGACTCGCGCCA
GGCCAAGGTCGACTTCGGCGCCGAGACCGGCACGAAGGACCTCCTCCTGCGCTACGCCCCGGTCGAGAAGCTCTAG

Upstream 100 bases:

>100_bases
CGGGGCATGGAAGTGTCGGACCCGCCCGGCAGCATGGCGTGGCGGTCGGCGTGGCCGCGGCGCCGGGTGGTTGGCCCGTC
GAACGTACGATCGAGGTCTG

Downstream 100 bases:

>100_bases
CCCGGAGGTCTGGCGGCGCGGCGGCGTGGGGTGAGGTCAGCCCGCGTCGGCTGTCGCCGACGCCGCGGTCGGCGGGGGTG
GAATCTGTTGTCGGTTGAGC

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 851; Mature: 850

Protein sequence:

>851_residues
MSTLFDDTFGPGALVDGGAPAGSGDGHEPHGDLPGGGRASRGAFGPAVLEPGGATRARAPRLDPDELLEGLNPQQRAAVV
HAGSPLLVVAGAGSGKTRVLTHRIAYLLAARGARPGEILAITFTNKAAGEMKERVEAIVGGRARAMWVSTFHSACVRILR
SEASRLGFGSSFSIYDAADAQRLITLVTRDLDLDPKRHTARGLAAAISALKNELVDWETARDRATNHIERTVAEVYAAYQ
QRLTQANALDFDDLIMTTVNLLQAFPDVAEHYRRRFRHVLVDEYQDTNHAQYVLIRELVGRSASSADEPAAGSGEAVYSG
GSAEPDGAGWPAKAVPPSGAVPSAELCVVGDADQSIYAFRGANIRNIVEFEQDFPNAAVILLEQNYRSTQTILSAANAVI
ARNAQRKPKRLWSDAGDGERIVGFVADNEHDEAAFVAEEIDRLGDENLARPADVAVFYRTNAQSRVFEEIFIRVGLPYRV
VGGVRFYERREIRDLLAYLRVLVNPTDTVNLRRILNVPRRGIGDRAEAMVSAFAERERIAFSEALSRVDEVPGVATRSAR
SIRDFVALLEGLREQLPAGPLAVIEATLERTGYLSELVAEDTIEAQGRVENLRELVGVVQEFTERRPDGTLAEFLEQVAL
VADADQIPDDDGSDGVVTLMTLHSAKGLEFPVVFLTGLEDGVFPHMRTLGDPTGLEEERRLAYVGVTRARARLYLTRSQV
RSAWGQPAYNPPSRFLGEVPDSLVEWRRLPEPAPAAGGGRFSGGGSAGAGNGSGGSGGKLPSSPFGAKPSRPAARPIIEL
TPGDRVTHDTFGLGVVVTTSGVGDSRQAKVDFGAETGTKDLLLRYAPVEKL

Sequences:

>Translated_851_residues
MSTLFDDTFGPGALVDGGAPAGSGDGHEPHGDLPGGGRASRGAFGPAVLEPGGATRARAPRLDPDELLEGLNPQQRAAVV
HAGSPLLVVAGAGSGKTRVLTHRIAYLLAARGARPGEILAITFTNKAAGEMKERVEAIVGGRARAMWVSTFHSACVRILR
SEASRLGFGSSFSIYDAADAQRLITLVTRDLDLDPKRHTARGLAAAISALKNELVDWETARDRATNHIERTVAEVYAAYQ
QRLTQANALDFDDLIMTTVNLLQAFPDVAEHYRRRFRHVLVDEYQDTNHAQYVLIRELVGRSASSADEPAAGSGEAVYSG
GSAEPDGAGWPAKAVPPSGAVPSAELCVVGDADQSIYAFRGANIRNIVEFEQDFPNAAVILLEQNYRSTQTILSAANAVI
ARNAQRKPKRLWSDAGDGERIVGFVADNEHDEAAFVAEEIDRLGDENLARPADVAVFYRTNAQSRVFEEIFIRVGLPYRV
VGGVRFYERREIRDLLAYLRVLVNPTDTVNLRRILNVPRRGIGDRAEAMVSAFAERERIAFSEALSRVDEVPGVATRSAR
SIRDFVALLEGLREQLPAGPLAVIEATLERTGYLSELVAEDTIEAQGRVENLRELVGVVQEFTERRPDGTLAEFLEQVAL
VADADQIPDDDGSDGVVTLMTLHSAKGLEFPVVFLTGLEDGVFPHMRTLGDPTGLEEERRLAYVGVTRARARLYLTRSQV
RSAWGQPAYNPPSRFLGEVPDSLVEWRRLPEPAPAAGGGRFSGGGSAGAGNGSGGSGGKLPSSPFGAKPSRPAARPIIEL
TPGDRVTHDTFGLGVVVTTSGVGDSRQAKVDFGAETGTKDLLLRYAPVEKL
>Mature_850_residues
STLFDDTFGPGALVDGGAPAGSGDGHEPHGDLPGGGRASRGAFGPAVLEPGGATRARAPRLDPDELLEGLNPQQRAAVVH
AGSPLLVVAGAGSGKTRVLTHRIAYLLAARGARPGEILAITFTNKAAGEMKERVEAIVGGRARAMWVSTFHSACVRILRS
EASRLGFGSSFSIYDAADAQRLITLVTRDLDLDPKRHTARGLAAAISALKNELVDWETARDRATNHIERTVAEVYAAYQQ
RLTQANALDFDDLIMTTVNLLQAFPDVAEHYRRRFRHVLVDEYQDTNHAQYVLIRELVGRSASSADEPAAGSGEAVYSGG
SAEPDGAGWPAKAVPPSGAVPSAELCVVGDADQSIYAFRGANIRNIVEFEQDFPNAAVILLEQNYRSTQTILSAANAVIA
RNAQRKPKRLWSDAGDGERIVGFVADNEHDEAAFVAEEIDRLGDENLARPADVAVFYRTNAQSRVFEEIFIRVGLPYRVV
GGVRFYERREIRDLLAYLRVLVNPTDTVNLRRILNVPRRGIGDRAEAMVSAFAERERIAFSEALSRVDEVPGVATRSARS
IRDFVALLEGLREQLPAGPLAVIEATLERTGYLSELVAEDTIEAQGRVENLRELVGVVQEFTERRPDGTLAEFLEQVALV
ADADQIPDDDGSDGVVTLMTLHSAKGLEFPVVFLTGLEDGVFPHMRTLGDPTGLEEERRLAYVGVTRARARLYLTRSQVR
SAWGQPAYNPPSRFLGEVPDSLVEWRRLPEPAPAAGGGRFSGGGSAGAGNGSGGSGGKLPSSPFGAKPSRPAARPIIELT
PGDRVTHDTFGLGVVVTTSGVGDSRQAKVDFGAETGTKDLLLRYAPVEKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=794, Percent_Identity=38.1612090680101, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI48994965, Length=684, Percent_Identity=36.6959064327485, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1787196, Length=400, Percent_Identity=24.5, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322369, Length=796, Percent_Identity=26.5075376884422, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324477, Length=711, Percent_Identity=22.0815752461322, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 91512; Mature: 91381

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLFDDTFGPGALVDGGAPAGSGDGHEPHGDLPGGGRASRGAFGPAVLEPGGATRARAP
CCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC
RLDPDELLEGLNPQQRAAVVHAGSPLLVVAGAGSGKTRVLTHRIAYLLAARGARPGEILA
CCCHHHHHHCCCHHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEE
ITFTNKAAGEMKERVEAIVGGRARAMWVSTFHSACVRILRSEASRLGFGSSFSIYDAADA
EEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHH
QRLITLVTRDLDLDPKRHTARGLAAAISALKNELVDWETARDRATNHIERTVAEVYAAYQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QRLTQANALDFDDLIMTTVNLLQAFPDVAEHYRRRFRHVLVDEYQDTNHAQYVLIRELVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
RSASSADEPAAGSGEAVYSGGSAEPDGAGWPAKAVPPSGAVPSAELCVVGDADQSIYAFR
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEC
GANIRNIVEFEQDFPNAAVILLEQNYRSTQTILSAANAVIARNAQRKPKRLWSDAGDGER
CCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCE
IVGFVADNEHDEAAFVAEEIDRLGDENLARPADVAVFYRTNAQSRVFEEIFIRVGLPYRV
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHH
VGGVRFYERREIRDLLAYLRVLVNPTDTVNLRRILNVPRRGIGDRAEAMVSAFAERERIA
HCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FSEALSRVDEVPGVATRSARSIRDFVALLEGLREQLPAGPLAVIEATLERTGYLSELVAE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
DTIEAQGRVENLRELVGVVQEFTERRPDGTLAEFLEQVALVADADQIPDDDGSDGVVTLM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHCCCCCCCCCCEEEEE
TLHSAKGLEFPVVFLTGLEDGVFPHMRTLGDPTGLEEERRLAYVGVTRARARLYLTRSQV
EECCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHH
RSAWGQPAYNPPSRFLGEVPDSLVEWRRLPEPAPAAGGGRFSGGGSAGAGNGSGGSGGKL
HHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PSSPFGAKPSRPAARPIIELTPGDRVTHDTFGLGVVVTTSGVGDSRQAKVDFGAETGTKD
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCHH
LLLRYAPVEKL
HHEECCCCCCC
>Mature Secondary Structure 
STLFDDTFGPGALVDGGAPAGSGDGHEPHGDLPGGGRASRGAFGPAVLEPGGATRARAP
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCC
RLDPDELLEGLNPQQRAAVVHAGSPLLVVAGAGSGKTRVLTHRIAYLLAARGARPGEILA
CCCHHHHHHCCCHHHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEE
ITFTNKAAGEMKERVEAIVGGRARAMWVSTFHSACVRILRSEASRLGFGSSFSIYDAADA
EEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHH
QRLITLVTRDLDLDPKRHTARGLAAAISALKNELVDWETARDRATNHIERTVAEVYAAYQ
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QRLTQANALDFDDLIMTTVNLLQAFPDVAEHYRRRFRHVLVDEYQDTNHAQYVLIRELVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
RSASSADEPAAGSGEAVYSGGSAEPDGAGWPAKAVPPSGAVPSAELCVVGDADQSIYAFR
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEC
GANIRNIVEFEQDFPNAAVILLEQNYRSTQTILSAANAVIARNAQRKPKRLWSDAGDGER
CCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCE
IVGFVADNEHDEAAFVAEEIDRLGDENLARPADVAVFYRTNAQSRVFEEIFIRVGLPYRV
EEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHH
VGGVRFYERREIRDLLAYLRVLVNPTDTVNLRRILNVPRRGIGDRAEAMVSAFAERERIA
HCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FSEALSRVDEVPGVATRSARSIRDFVALLEGLREQLPAGPLAVIEATLERTGYLSELVAE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
DTIEAQGRVENLRELVGVVQEFTERRPDGTLAEFLEQVALVADADQIPDDDGSDGVVTLM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHCCCCCCCCCCEEEEE
TLHSAKGLEFPVVFLTGLEDGVFPHMRTLGDPTGLEEERRLAYVGVTRARARLYLTRSQV
EECCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHH
RSAWGQPAYNPPSRFLGEVPDSLVEWRRLPEPAPAAGGGRFSGGGSAGAGNGSGGSGGKL
HHHCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PSSPFGAKPSRPAARPIIELTPGDRVTHDTFGLGVVVTTSGVGDSRQAKVDFGAETGTKD
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCHH
LLLRYAPVEKL
HHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]