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The map label for this gene is punA [H]

Identifier: 158317667

GI number: 158317667

Start: 7195589

End: 7196635

Strand: Reverse

Name: punA [H]

Synonym: Franean1_5925

Alternate gene names: 158317667

Gene position: 7196635-7195589 (Counterclockwise)

Preceding gene: 158317671

Following gene: 158317665

Centisome position: 80.12

GC content: 74.5

Gene sequence:

>1047_bases
ATGCCCGACGAAGCGCTCGGCCTTCGCGGTGACTCGCCCGCCACCGGCCGGGTCCGTCATCCGACCGCGCAGCACGACCT
CGATGAGCTCTCCGTCCTCAAGCGGCACCCGCGGGGGCTTCCGGACGGAGGGGACGAGGGACACCTCACCAGTGTGCTCC
CGAAACGCCAGGCGGTGCCGGGCCGGGGCCACGCGGGAGTGGTATGCCGCGAGTCGTACAGTCGCCTGGTGGCGGACAGC
GACATCATCAGCGCCGCCGCCGACGCCGCGGCGTCGGCGGCCTGCCTGGCCGAGCGCACCGGGGCCGACCGGCACGACGT
CGCGATCGTCCTCGGGTCCGGCTGGCGGCCCGCGGCGGACGTCCTGGCTGGTCTCGGCACCGACGTCGTGGACGTCGACT
TCGCCGACCTGGGCGGCTTCCCCGCCACGGATGTTCCCGGGCACGCGCCCACGGCCCGTTCCATCCTGCTCGGCGGGCGG
CGGCTGCTGGTCTTCCTCGGCCGGGTGCACGCCTACGAGGGCCACGACCTCTCCCGGGTCGTGCACACCGTCCGCACCGC
GCACGCCGCCGGCGCGGGCACCGTCGTCCTGACGAACGCGGCCGGTGGGCTGCGTTCCGACCTGCACGTGGGCCAACCGG
TGCTGGTCGCCGACCACCTCAACCTGACCGGCCGCTCGCCTCTGGTCGGCCCGCTGTTCACCGACCTCACCGACGCCTAC
TCGCCCCGGTTGCGCGCTCTCGCCCGCACCGTGGAGCCGAGCCTGGTCGAAGGGATCTACGCGGCGCTGCCCGGCCCGCA
CTTCGAGACGCCGGCCGAGATCCGCATGCTGCGCGGGCTCGGTGCCGACCTCGTCGGCATGTCGACGGTGCACGAGACGA
TCGCCGCCCGGGCGCTCGGCATCGACGTGCTCGCGCTCTCGCTGGTCACGAACCTGGCCGCCGGGATGACCGGCGCGCCA
CTGGACCACACCGAGGTCCTCGCCGCCGGCGCCGCGGCGGCCGGCCGGATGGGCTCGATGCTCGCCGAGGTCATCACCGC
GCTTTGA

Upstream 100 bases:

>100_bases
GGTCGTAGGAGACGTCGAACCAGTCGCCCTCCCGCGGGAAGGGCGCGACCACCAGCAGCCGCCGCGTGGTCAGCATGATC
CAGCGGGACGTGCCGAGGCG

Downstream 100 bases:

>100_bases
CATCCCTCCCCCGCGTGAACGCGGGGATTCCAACCCCTACACACCCGTCGGAAGGGGTGCGCAGTGTTGAGGTTCGCGGT
TCGCGGGCCCGGCCAACGCC

Product: purine nucleotide phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MPDEALGLRGDSPATGRVRHPTAQHDLDELSVLKRHPRGLPDGGDEGHLTSVLPKRQAVPGRGHAGVVCRESYSRLVADS
DIISAAADAAASAACLAERTGADRHDVAIVLGSGWRPAADVLAGLGTDVVDVDFADLGGFPATDVPGHAPTARSILLGGR
RLLVFLGRVHAYEGHDLSRVVHTVRTAHAAGAGTVVLTNAAGGLRSDLHVGQPVLVADHLNLTGRSPLVGPLFTDLTDAY
SPRLRALARTVEPSLVEGIYAALPGPHFETPAEIRMLRGLGADLVGMSTVHETIAARALGIDVLALSLVTNLAAGMTGAP
LDHTEVLAAGAAAAGRMGSMLAEVITAL

Sequences:

>Translated_348_residues
MPDEALGLRGDSPATGRVRHPTAQHDLDELSVLKRHPRGLPDGGDEGHLTSVLPKRQAVPGRGHAGVVCRESYSRLVADS
DIISAAADAAASAACLAERTGADRHDVAIVLGSGWRPAADVLAGLGTDVVDVDFADLGGFPATDVPGHAPTARSILLGGR
RLLVFLGRVHAYEGHDLSRVVHTVRTAHAAGAGTVVLTNAAGGLRSDLHVGQPVLVADHLNLTGRSPLVGPLFTDLTDAY
SPRLRALARTVEPSLVEGIYAALPGPHFETPAEIRMLRGLGADLVGMSTVHETIAARALGIDVLALSLVTNLAAGMTGAP
LDHTEVLAAGAAAAGRMGSMLAEVITAL
>Mature_347_residues
PDEALGLRGDSPATGRVRHPTAQHDLDELSVLKRHPRGLPDGGDEGHLTSVLPKRQAVPGRGHAGVVCRESYSRLVADSD
IISAAADAAASAACLAERTGADRHDVAIVLGSGWRPAADVLAGLGTDVVDVDFADLGGFPATDVPGHAPTARSILLGGRR
LLVFLGRVHAYEGHDLSRVVHTVRTAHAAGAGTVVLTNAAGGLRSDLHVGQPVLVADHLNLTGRSPLVGPLFTDLTDAYS
PRLRALARTVEPSLVEGIYAALPGPHFETPAEIRMLRGLGADLVGMSTVHETIAARALGIDVLALSLVTNLAAGMTGAPL
DHTEVLAAGAAAAGRMGSMLAEVITAL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=279, Percent_Identity=37.9928315412186, Blast_Score=158, Evalue=6e-39,
Organism=Escherichia coli, GI1788746, Length=234, Percent_Identity=37.6068376068376, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17541190, Length=280, Percent_Identity=35.7142857142857, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323238, Length=270, Percent_Identity=35.1851851851852, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI45552885, Length=248, Percent_Identity=35.4838709677419, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24656093, Length=248, Percent_Identity=35.4838709677419, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24656090, Length=248, Percent_Identity=35.4838709677419, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI45552887, Length=248, Percent_Identity=35.4838709677419, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24762376, Length=233, Percent_Identity=28.755364806867, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 35773; Mature: 35642

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDEALGLRGDSPATGRVRHPTAQHDLDELSVLKRHPRGLPDGGDEGHLTSVLPKRQAVP
CCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHCCC
GRGHAGVVCRESYSRLVADSDIISAAADAAASAACLAERTGADRHDVAIVLGSGWRPAAD
CCCCCCEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
VLAGLGTDVVDVDFADLGGFPATDVPGHAPTARSILLGGRRLLVFLGRVHAYEGHDLSRV
HHHHCCCCEEEECHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
VHTVRTAHAAGAGTVVLTNAAGGLRSDLHVGQPVLVADHLNLTGRSPLVGPLFTDLTDAY
HHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
SPRLRALARTVEPSLVEGIYAALPGPHFETPAEIRMLRGLGADLVGMSTVHETIAARALG
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IDVLALSLVTNLAAGMTGAPLDHTEVLAAGAAAAGRMGSMLAEVITAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PDEALGLRGDSPATGRVRHPTAQHDLDELSVLKRHPRGLPDGGDEGHLTSVLPKRQAVP
CCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHCCC
GRGHAGVVCRESYSRLVADSDIISAAADAAASAACLAERTGADRHDVAIVLGSGWRPAAD
CCCCCCEEEHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHH
VLAGLGTDVVDVDFADLGGFPATDVPGHAPTARSILLGGRRLLVFLGRVHAYEGHDLSRV
HHHHCCCCEEEECHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHH
VHTVRTAHAAGAGTVVLTNAAGGLRSDLHVGQPVLVADHLNLTGRSPLVGPLFTDLTDAY
HHHHHHHHCCCCCEEEEEECCCCCCCHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
SPRLRALARTVEPSLVEGIYAALPGPHFETPAEIRMLRGLGADLVGMSTVHETIAARALG
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IDVLALSLVTNLAAGMTGAPLDHTEVLAAGAAAAGRMGSMLAEVITAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]