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The map label for this gene is prfB [H]

Identifier: 158317542

GI number: 158317542

Start: 7040659

End: 7041819

Strand: Reverse

Name: prfB [H]

Synonym: Franean1_5798

Alternate gene names: 158317542

Gene position: 7041819-7040659 (Counterclockwise)

Preceding gene: 158317544

Following gene: 158317541

Centisome position: 78.4

GC content: 68.56

Gene sequence:

>1161_bases
GTGGAGGGCCTGGATCAACCGTTCGTCGGCTCCCGCGTGCACGCCGGTAGGCTGGGTCACATGGCCGTCGACCTCTCCGA
GGAGATCAAGAATCTCGACGCGACGCTGGCAGGTATCGCGACCGTGCTCGATGTCGAGGGTCTGCGTCGCCGCGCTCAGG
CGCTGGAACAGGAGGCGGCCGACCCGGACCTGTGGAGCGACCAGGATCGCGCCCAGGCGGTGACGAGGCGGCTGTCCGCT
GTGAAGGGTGACATCGGCCGGGTGGAGGGCCTGCGCCGCAGGCTCGACGACCTCATCGCCGCCCTCGACCTCGGCGACGA
CGACCTGCTCACCGAGGCGGCCGCAGACCTCCCCGCGCTGGCGCGTGACATCTCCACGCTCGAGGTCCGCACCCTGCTCT
CCGGCGAGTACGACGAGCGTGACGCGATCGTCCAGCTCTCGGCTGGCGCGGGCGGGGTGGACGCGGCCGACTGGACCGCC
ATGCTGCTGCGGATGTACCTGCGCTGGGCCGAGCGGCACGGGTACGCCACCGAGGTCTTCGACACCTCCGAGGCGGAGGA
GGCCGGTCTGAAGTCGGCCACCTTCCAGGTCAAGTGCCCCTACGCCTACGGAACGCTGCGCAGCGAGCACGGTGTGCACC
GGCTGGTGCGCATCTCGCCCTTCGACAACCAGAACCGGCGCCAGACGTCGTTCGCCGGCGTCGAGGTGACTCCCGCCGTC
GAGGTCTCCGACCACGTGGACATCGACGAGAAGGAGCTCCGGATCGACATCTTCCGGTCCTCCGGACCGGGTGGCCAGGG
CGTCAACACCACCGACTCCGCCGTGCGGATCACGCACCTGCCCACCAACATCGTCGTGACCTGCCAGAACGAGCGCAGCC
AGCTGCAGAACAAGGCCGCCGCGATGATGGTCCTGCAGGCCAAGCTGCTCGAGCGGCGCCGCGCCGAGGAGGCCGCGGAG
AAGCAGCGCCTCACCGGCGGCCCGCAGGATGTCTCCTTCGGTTCACAGATCCGGAACTACGTGCTCCACCCGTACCAGAT
GGTCAAGGATCTGCGGACCGAGGTCGAGACGTCGAACACGAACGCCGTTCTCGACGGGGATATCGACGACTTCATCGACG
GTGAGATCAGGTGGCGCAGGGCCACCGAAAAGCAGGGCTGA

Upstream 100 bases:

>100_bases
GGATCCGGCCCGGTGGTGCCGCCGGTCCCGCGCGTGGGAGACGCGGGAGAGCCGCTCCGGGCCGGGCGCCGGCTGATCGC
GGCAGCCCGCGGGCGGGCTG

Downstream 100 bases:

>100_bases
CAGGTCTTTTCCGTGGCTTCCGGCCATGATTCGGTAATCCCACCGGTCGTTATCAGTCGGAAAGCCGCGGACTTTTCCCA
GCCAATCCCGTTCGCTGGCG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEGLDQPFVGSRVHAGRLGHMAVDLSEEIKNLDATLAGIATVLDVEGLRRRAQALEQEAADPDLWSDQDRAQAVTRRLSA
VKGDIGRVEGLRRRLDDLIAALDLGDDDLLTEAAADLPALARDISTLEVRTLLSGEYDERDAIVQLSAGAGGVDAADWTA
MLLRMYLRWAERHGYATEVFDTSEAEEAGLKSATFQVKCPYAYGTLRSEHGVHRLVRISPFDNQNRRQTSFAGVEVTPAV
EVSDHVDIDEKELRIDIFRSSGPGGQGVNTTDSAVRITHLPTNIVVTCQNERSQLQNKAAAMMVLQAKLLERRRAEEAAE
KQRLTGGPQDVSFGSQIRNYVLHPYQMVKDLRTEVETSNTNAVLDGDIDDFIDGEIRWRRATEKQG

Sequences:

>Translated_386_residues
MEGLDQPFVGSRVHAGRLGHMAVDLSEEIKNLDATLAGIATVLDVEGLRRRAQALEQEAADPDLWSDQDRAQAVTRRLSA
VKGDIGRVEGLRRRLDDLIAALDLGDDDLLTEAAADLPALARDISTLEVRTLLSGEYDERDAIVQLSAGAGGVDAADWTA
MLLRMYLRWAERHGYATEVFDTSEAEEAGLKSATFQVKCPYAYGTLRSEHGVHRLVRISPFDNQNRRQTSFAGVEVTPAV
EVSDHVDIDEKELRIDIFRSSGPGGQGVNTTDSAVRITHLPTNIVVTCQNERSQLQNKAAAMMVLQAKLLERRRAEEAAE
KQRLTGGPQDVSFGSQIRNYVLHPYQMVKDLRTEVETSNTNAVLDGDIDDFIDGEIRWRRATEKQG
>Mature_386_residues
MEGLDQPFVGSRVHAGRLGHMAVDLSEEIKNLDATLAGIATVLDVEGLRRRAQALEQEAADPDLWSDQDRAQAVTRRLSA
VKGDIGRVEGLRRRLDDLIAALDLGDDDLLTEAAADLPALARDISTLEVRTLLSGEYDERDAIVQLSAGAGGVDAADWTA
MLLRMYLRWAERHGYATEVFDTSEAEEAGLKSATFQVKCPYAYGTLRSEHGVHRLVRISPFDNQNRRQTSFAGVEVTPAV
EVSDHVDIDEKELRIDIFRSSGPGGQGVNTTDSAVRITHLPTNIVVTCQNERSQLQNKAAAMMVLQAKLLERRRAEEAAE
KQRLTGGPQDVSFGSQIRNYVLHPYQMVKDLRTEVETSNTNAVLDGDIDDFIDGEIRWRRATEKQG

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=275, Percent_Identity=34.9090909090909, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI34577120, Length=297, Percent_Identity=31.3131313131313, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=36.6120218579235, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=46.0176991150442, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=34.6590909090909, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17542784, Length=200, Percent_Identity=34.5, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=206, Percent_Identity=37.8640776699029, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19921226, Length=229, Percent_Identity=37.9912663755458, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42633; Mature: 42633

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLDQPFVGSRVHAGRLGHMAVDLSEEIKNLDATLAGIATVLDVEGLRRRAQALEQEAA
CCCCCCCCCCCCCCCCCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPDLWSDQDRAQAVTRRLSAVKGDIGRVEGLRRRLDDLIAALDLGDDDLLTEAAADLPAL
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ARDISTLEVRTLLSGEYDERDAIVQLSAGAGGVDAADWTAMLLRMYLRWAERHGYATEVF
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEC
DTSEAEEAGLKSATFQVKCPYAYGTLRSEHGVHRLVRISPFDNQNRRQTSFAGVEVTPAV
CCCCHHHCCCCCCEEEEECCHHHCCHHCCCCCEEEEEECCCCCCCCCCHHCCCEEECCCE
EVSDHVDIDEKELRIDIFRSSGPGGQGVNTTDSAVRITHLPTNIVVTCQNERSQLQNKAA
ECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHH
AMMVLQAKLLERRRAEEAAEKQRLTGGPQDVSFGSQIRNYVLHPYQMVKDLRTEVETSNT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
NAVLDGDIDDFIDGEIRWRRATEKQG
CEEECCCHHHHCCCCEEEECCCCCCC
>Mature Secondary Structure
MEGLDQPFVGSRVHAGRLGHMAVDLSEEIKNLDATLAGIATVLDVEGLRRRAQALEQEAA
CCCCCCCCCCCCCCCCCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPDLWSDQDRAQAVTRRLSAVKGDIGRVEGLRRRLDDLIAALDLGDDDLLTEAAADLPAL
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ARDISTLEVRTLLSGEYDERDAIVQLSAGAGGVDAADWTAMLLRMYLRWAERHGYATEVF
HHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEC
DTSEAEEAGLKSATFQVKCPYAYGTLRSEHGVHRLVRISPFDNQNRRQTSFAGVEVTPAV
CCCCHHHCCCCCCEEEEECCHHHCCHHCCCCCEEEEEECCCCCCCCCCHHCCCEEECCCE
EVSDHVDIDEKELRIDIFRSSGPGGQGVNTTDSAVRITHLPTNIVVTCQNERSQLQNKAA
ECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHHHHHHHH
AMMVLQAKLLERRRAEEAAEKQRLTGGPQDVSFGSQIRNYVLHPYQMVKDLRTEVETSNT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
NAVLDGDIDDFIDGEIRWRRATEKQG
CEEECCCHHHHCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA