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The map label for this gene is yhxA [H]

Identifier: 158317470

GI number: 158317470

Start: 6945719

End: 6947059

Strand: Reverse

Name: yhxA [H]

Synonym: Franean1_5722

Alternate gene names: 158317470

Gene position: 6947059-6945719 (Counterclockwise)

Preceding gene: 158317472

Following gene: 158317468

Centisome position: 77.34

GC content: 69.8

Gene sequence:

>1341_bases
ATGACGCAGGCGAGCTCGGCCCACCCGGAGAACCGCGAGCACGTGTTCTACTCCTGGGCGGCGCAGGAGACGACGCACCC
GCTCGTGGTCGCAGGGGCCGAGGGGAGCTGGTTCTGGGACAAGGGTGGCACACGGTACCTCGACTTCTCCTCCCAGCTGG
TGAACGCCAACATCGGCCACCAGCATCCGGCGGTGGTCGAGGCGGTCGTGGCGCAGGCCCGGGAGCTGACCACGGTCGGG
CCGCAGCACGCCCACGCCGTGCGCTCGGAGGCGGCGCGGCTGATCGCCGAGCGTGCTCCCGGCGATCTGGACCAGGTGTT
CTTCACCACCGGGGGAGCGGAGGCCACGGAGAACGCCGTGCGGCTGGCCCGGCTCGCCACCGGGCGGAACAAGATCCTCG
CCGCCTACCGGTCGTACCACGGTTCGACCGGCGGCTCGATCACGCTGACCGGGGAGCCGCGGCGTTGGGCGAGCGAACCG
GGTATTCCCGGTGTGGTGCACTTCTTCGGCCCCTATCCGTACCGGTCGTCTTTCCACGCCGCCGACGCGGCTCAGGAGGG
GGAGCGCGCGCTCGCGCACCTCGCAGAGGTGATCGAGCTGGAGGGGCCGTCGGCGATCGCCGCGGTCATCCTCGAGCCGG
TCGTCGGTACGAACGGGATCCTGGTGCCGCCGGACGGGTACCTCGCGGGCGTCCGGGAGTTGTGCGACGCGCACGGAATA
CTGCTGATCGCCGACGAGGTGATGTCGGGTTTCGGGCGGTGCGGGGAATGGTTCGCGATCGACCACTGGAATGTGGTGCC
CGATCTGATCTGTTTCGCGAAGGGCGTCAACTCGGGTTATGTGCCGCTTGGCGGGGTGATTATTTCGCAGCGGATCGCGG
ACCTGTTCGCGCGGCGGCCGTATCCTGGCGGGCTGACCTATTCGGGGCATCTGCTTGCCTGCGCGGCGGCGGTGGCGTCC
ATCAGGGCTTTCGAGTCCGAGGACATTCTCGGCCGGGCCCGTGCGCTCGGTTCCGAGGTTATCGGGCCGCAACTGGCTAA
AATTGCCGCGCGGCATCCCAGCGTGGGTGAGGTGCGCGGGCTCGGGGTGTTCTGGGCGGTCGAGCTCGTCCGTGACCGGG
TTACCCGGGAGCCGTTGGTTCCGTTCAACGCGGCGGGTGCCGCGGCCGCGCCGATGGCGGCCGTGACGGCGGCCTGCCGG
GAACGCGGCCTCTGGCCGTTCACCCATTTCAACCGGGTGCATGTCGTGCCGCCGTGCACCACGAGTCCCGAGGATGTCTC
CCTCGGCCTGTCGATCCTGGACGAGGCCCTGGCCGTCGCGGACGGCTGCTACACCGGGTAA

Upstream 100 bases:

>100_bases
GGCGGCGGGAAATCGGGCTGGTGGCGGGAAATACTGCCGGGCAGCTGGCGCGTGGGACGGCTGCCGCGAGCTGGGTTGGT
CCGCAGACAGGGAGACGATG

Downstream 100 bases:

>100_bases
CCCGCGCCCGGGGCGTCGGCCGGCCCGCCTCCCCCGTCCCCGCCGTCGGGGCAACCAGACCGGGCCCCCAAACGGCGGAT
GCCCGGCCTGGTTCGCCCCT

Product: hypothetical protein

Products: L-Glutamate; Succinic semialdehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTQASSAHPENREHVFYSWAAQETTHPLVVAGAEGSWFWDKGGTRYLDFSSQLVNANIGHQHPAVVEAVVAQARELTTVG
PQHAHAVRSEAARLIAERAPGDLDQVFFTTGGAEATENAVRLARLATGRNKILAAYRSYHGSTGGSITLTGEPRRWASEP
GIPGVVHFFGPYPYRSSFHAADAAQEGERALAHLAEVIELEGPSAIAAVILEPVVGTNGILVPPDGYLAGVRELCDAHGI
LLIADEVMSGFGRCGEWFAIDHWNVVPDLICFAKGVNSGYVPLGGVIISQRIADLFARRPYPGGLTYSGHLLACAAAVAS
IRAFESEDILGRARALGSEVIGPQLAKIAARHPSVGEVRGLGVFWAVELVRDRVTREPLVPFNAAGAAAAPMAAVTAACR
ERGLWPFTHFNRVHVVPPCTTSPEDVSLGLSILDEALAVADGCYTG

Sequences:

>Translated_446_residues
MTQASSAHPENREHVFYSWAAQETTHPLVVAGAEGSWFWDKGGTRYLDFSSQLVNANIGHQHPAVVEAVVAQARELTTVG
PQHAHAVRSEAARLIAERAPGDLDQVFFTTGGAEATENAVRLARLATGRNKILAAYRSYHGSTGGSITLTGEPRRWASEP
GIPGVVHFFGPYPYRSSFHAADAAQEGERALAHLAEVIELEGPSAIAAVILEPVVGTNGILVPPDGYLAGVRELCDAHGI
LLIADEVMSGFGRCGEWFAIDHWNVVPDLICFAKGVNSGYVPLGGVIISQRIADLFARRPYPGGLTYSGHLLACAAAVAS
IRAFESEDILGRARALGSEVIGPQLAKIAARHPSVGEVRGLGVFWAVELVRDRVTREPLVPFNAAGAAAAPMAAVTAACR
ERGLWPFTHFNRVHVVPPCTTSPEDVSLGLSILDEALAVADGCYTG
>Mature_445_residues
TQASSAHPENREHVFYSWAAQETTHPLVVAGAEGSWFWDKGGTRYLDFSSQLVNANIGHQHPAVVEAVVAQARELTTVGP
QHAHAVRSEAARLIAERAPGDLDQVFFTTGGAEATENAVRLARLATGRNKILAAYRSYHGSTGGSITLTGEPRRWASEPG
IPGVVHFFGPYPYRSSFHAADAAQEGERALAHLAEVIELEGPSAIAAVILEPVVGTNGILVPPDGYLAGVRELCDAHGIL
LIADEVMSGFGRCGEWFAIDHWNVVPDLICFAKGVNSGYVPLGGVIISQRIADLFARRPYPGGLTYSGHLLACAAAVASI
RAFESEDILGRARALGSEVIGPQLAKIAARHPSVGEVRGLGVFWAVELVRDRVTREPLVPFNAAGAAAAPMAAVTAACRE
RGLWPFTHFNRVHVVPPCTTSPEDVSLGLSILDEALAVADGCYTG

Specific function: Essential for glycerol catabolism [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=439, Percent_Identity=28.4738041002278, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI226442705, Length=437, Percent_Identity=28.1464530892449, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI4557809, Length=452, Percent_Identity=28.0973451327434, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI226442709, Length=393, Percent_Identity=29.2620865139949, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI13994255, Length=436, Percent_Identity=27.7522935779816, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI24119277, Length=362, Percent_Identity=30.1104972375691, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI284507298, Length=337, Percent_Identity=28.1899109792285, Blast_Score=119, Evalue=8e-27,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=35.1543942992874, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1787560, Length=417, Percent_Identity=35.2517985611511, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI145693181, Length=406, Percent_Identity=29.8029556650246, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788044, Length=412, Percent_Identity=28.6407766990291, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789759, Length=342, Percent_Identity=29.5321637426901, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1786349, Length=297, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1786991, Length=432, Percent_Identity=28.0092592592593, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564660, Length=438, Percent_Identity=28.9954337899543, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71992977, Length=447, Percent_Identity=27.2930648769575, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25144271, Length=417, Percent_Identity=26.6187050359712, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144274, Length=304, Percent_Identity=25.9868421052632, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17541228, Length=381, Percent_Identity=24.1469816272966, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=439, Percent_Identity=30.751708428246, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324432, Length=423, Percent_Identity=24.3498817966903, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324386, Length=311, Percent_Identity=27.9742765273312, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161085790, Length=433, Percent_Identity=30.4849884526559, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28574759, Length=437, Percent_Identity=29.7482837528604, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21356575, Length=364, Percent_Identity=31.8681318681319, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI21357415, Length=449, Percent_Identity=25.1670378619154, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI281366494, Length=389, Percent_Identity=20.8226221079692, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667139, Length=389, Percent_Identity=20.8226221079692, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667143, Length=389, Percent_Identity=20.8226221079692, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19 [C]

Molecular weight: Translated: 47354; Mature: 47223

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQASSAHPENREHVFYSWAAQETTHPLVVAGAEGSWFWDKGGTRYLDFSSQLVNANIGH
CCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEECCCCCEEEECCHHHHCCCCCC
QHPAVVEAVVAQARELTTVGPQHAHAVRSEAARLIAERAPGDLDQVFFTTGGAEATENAV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCHHHHHHH
RLARLATGRNKILAAYRSYHGSTGGSITLTGEPRRWASEPGIPGVVHFFGPYPYRSSFHA
HHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCHHCCCCCCCEEEEECCCCCCCCCCCH
ADAAQEGERALAHLAEVIELEGPSAIAAVILEPVVGTNGILVPPDGYLAGVRELCDAHGI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCE
LLIADEVMSGFGRCGEWFAIDHWNVVPDLICFAKGVNSGYVPLGGVIISQRIADLFARRP
EEEHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
YPGGLTYSGHLLACAAAVASIRAFESEDILGRARALGSEVIGPQLAKIAARHPSVGEVRG
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHC
LGVFWAVELVRDRVTREPLVPFNAAGAAAAPMAAVTAACRERGLWPFTHFNRVHVVPPCT
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
TSPEDVSLGLSILDEALAVADGCYTG
CCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQASSAHPENREHVFYSWAAQETTHPLVVAGAEGSWFWDKGGTRYLDFSSQLVNANIGH
CCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCEEECCCCCEEEECCHHHHCCCCCC
QHPAVVEAVVAQARELTTVGPQHAHAVRSEAARLIAERAPGDLDQVFFTTGGAEATENAV
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCHHHHHHH
RLARLATGRNKILAAYRSYHGSTGGSITLTGEPRRWASEPGIPGVVHFFGPYPYRSSFHA
HHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCHHCCCCCCCEEEEECCCCCCCCCCCH
ADAAQEGERALAHLAEVIELEGPSAIAAVILEPVVGTNGILVPPDGYLAGVRELCDAHGI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCE
LLIADEVMSGFGRCGEWFAIDHWNVVPDLICFAKGVNSGYVPLGGVIISQRIADLFARRP
EEEHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
YPGGLTYSGHLLACAAAVASIRAFESEDILGRARALGSEVIGPQLAKIAARHPSVGEVRG
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHC
LGVFWAVELVRDRVTREPLVPFNAAGAAAAPMAAVTAACRERGLWPFTHFNRVHVVPPCT
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
TSPEDVSLGLSILDEALAVADGCYTG
CCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-Aminobutanoate; 2-Oxoglutarate [C]

Specific reaction: 4-Aminobutanoate + 2-Oxoglutarate --> L-Glutamate + Succinic semialdehyde [C]

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8969498; 8436953 [H]