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The map label for this gene is rph

Identifier: 158317399

GI number: 158317399

Start: 6863336

End: 6864118

Strand: Direct

Name: rph

Synonym: Franean1_5650

Alternate gene names: 158317399

Gene position: 6863336-6864118 (Clockwise)

Preceding gene: 158317398

Following gene: 158317400

Centisome position: 76.41

GC content: 74.2

Gene sequence:

>783_bases
GTGATCAGAGTCGATGGCAGAACCACGGACCAGCTCCGCGACGTGACGATCGAGCGGGGCTGGCAGGAGCACGCGGAGGG
GTCGGCGCTGATCTCCGCCGGCCGGACCAGGGTGCTGTGCGCGGCCTCCGTCACCGAGGGGGTGCCCCGGTGGCGCAAGG
GCAGCGGCCTGGGCTGGGTGACGGCCGAGTACTCGATGCTGCCGCGAGCCACGCACACCCGCAACGACCGGGAGTCCGTC
CGCGGGCGGATCGGCGGGCGGACCCACGAGATCTCCCGGCTGATCGGGCGCAGCCTGCGGGCCGCGATCGACCTGAAGGC
CCTCGGCGAGAACACCATCGCCATCGACTGTGACGTGCTGCTCGCCGACGGCGGCACCCGCACGGCCGCGATCACCGGCG
CCTATGTGGCGCTCGCCGACGCCTGCCGGTGGCTGGGACGTCCCGCGGCGATCGTCACCAGTGTCTGCGCGGTCAGCGTC
GGCGTGGTCGGCGGCGTGCCCATGCTCGACCTCGCCTACACCGAGGACTCGACCGCCGACACGGACATGAACGTCGTCCG
CACCGGCGCGGGTGGCTTCGTCGAGGTCCAGGGGACGGCCGAGGGCACCCCGTTCGAACGGTCGACGCTCGACGAGCTGC
TCGACCTCGCCGTGGCCGGCTGCACCCGCCTGACCGAGATCCAGAACGCCGCGCTGGCCGCCCCGCCGGCCGCCGGCCCG
CCCGCCCCGGAACGCGCCGGCGCCGGCAGTGGCAGTGGCGGGAAGGGCACGGGGTCACGGTGA

Upstream 100 bases:

>100_bases
GCACCTCCTACGAGATCTGAACACGTCTGAGCACGTCTGAGCACGCACGCGGGCCGTCCGGCGCAACCCCGGCCGGCCTG
CGCCGATAGGGTCTCCTCCC

Downstream 100 bases:

>100_bases
GCGCGGTGCCGCGCCAGGTCGTGCTCGCCAGCCGCAACGAGGCGAAACTGGCCGAGCTGGGCCGCATCCTCGCCGCGACC
GGGCTGGACGTCGATGTCGT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MIRVDGRTTDQLRDVTIERGWQEHAEGSALISAGRTRVLCAASVTEGVPRWRKGSGLGWVTAEYSMLPRATHTRNDRESV
RGRIGGRTHEISRLIGRSLRAAIDLKALGENTIAIDCDVLLADGGTRTAAITGAYVALADACRWLGRPAAIVTSVCAVSV
GVVGGVPMLDLAYTEDSTADTDMNVVRTGAGGFVEVQGTAEGTPFERSTLDELLDLAVAGCTRLTEIQNAALAAPPAAGP
PAPERAGAGSGSGGKGTGSR

Sequences:

>Translated_260_residues
MIRVDGRTTDQLRDVTIERGWQEHAEGSALISAGRTRVLCAASVTEGVPRWRKGSGLGWVTAEYSMLPRATHTRNDRESV
RGRIGGRTHEISRLIGRSLRAAIDLKALGENTIAIDCDVLLADGGTRTAAITGAYVALADACRWLGRPAAIVTSVCAVSV
GVVGGVPMLDLAYTEDSTADTDMNVVRTGAGGFVEVQGTAEGTPFERSTLDELLDLAVAGCTRLTEIQNAALAAPPAAGP
PAPERAGAGSGSGGKGTGSR
>Mature_260_residues
MIRVDGRTTDQLRDVTIERGWQEHAEGSALISAGRTRVLCAASVTEGVPRWRKGSGLGWVTAEYSMLPRATHTRNDRESV
RGRIGGRTHEISRLIGRSLRAAIDLKALGENTIAIDCDVLLADGGTRTAAITGAYVALADACRWLGRPAAIVTSVCAVSV
GVVGGVPMLDLAYTEDSTADTDMNVVRTGAGGFVEVQGTAEGTPFERSTLDELLDLAVAGCTRLTEIQNAALAAPPAAGP
PAPERAGAGSGSGGKGTGSR

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=60.7655502392344, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71981632, Length=181, Percent_Identity=32.5966850828729, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_FRASN (A8L766)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001509907.1
- ProteinModelPortal:   A8L766
- SMR:   A8L766
- GeneID:   5673977
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_5650
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   FSP1855:FRANEAN1_5650-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26939; Mature: 26939

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVDGRTTDQLRDVTIERGWQEHAEGSALISAGRTRVLCAASVTEGVPRWRKGSGLGWV
CEEECCCCHHHHHHHHHHCCHHHHCCCCEEECCCCCEEEEEEHHHHCCCCCCCCCCCEEE
TAEYSMLPRATHTRNDRESVRGRIGGRTHEISRLIGRSLRAAIDLKALGENTIAIDCDVL
EEHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
LADGGTRTAAITGAYVALADACRWLGRPAAIVTSVCAVSVGVVGGVPMLDLAYTEDSTAD
EECCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
TDMNVVRTGAGGFVEVQGTAEGTPFERSTLDELLDLAVAGCTRLTEIQNAALAAPPAAGP
CCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAPERAGAGSGSGGKGTGSR
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRVDGRTTDQLRDVTIERGWQEHAEGSALISAGRTRVLCAASVTEGVPRWRKGSGLGWV
CEEECCCCHHHHHHHHHHCCHHHHCCCCEEECCCCCEEEEEEHHHHCCCCCCCCCCCEEE
TAEYSMLPRATHTRNDRESVRGRIGGRTHEISRLIGRSLRAAIDLKALGENTIAIDCDVL
EEHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEE
LADGGTRTAAITGAYVALADACRWLGRPAAIVTSVCAVSVGVVGGVPMLDLAYTEDSTAD
EECCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
TDMNVVRTGAGGFVEVQGTAEGTPFERSTLDELLDLAVAGCTRLTEIQNAALAAPPAAGP
CCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAPERAGAGSGSGGKGTGSR
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA