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The map label for this gene is mcrA [H]

Identifier: 158317152

GI number: 158317152

Start: 6513548

End: 6514984

Strand: Reverse

Name: mcrA [H]

Synonym: Franean1_5399

Alternate gene names: 158317152

Gene position: 6514984-6513548 (Counterclockwise)

Preceding gene: 158317153

Following gene: 158317149

Centisome position: 72.53

GC content: 69.45

Gene sequence:

>1437_bases
ATGTCGGCACAGGTTCTCACGCTGGAAGGCCAGCGGCAGAGACTCTCGGACGAGACGGTCGAGGAGATCCGTGCGATCTT
CCACGGGCAGGTTCTGACCTCGGACGACGTCGGGTACGACGACGTCCGGGTCATCCAGAACGCCATGCTCGACCGCAGGC
CAGGTCTGATCATCCGGTGTACCGGGGCCGCCGACGTCGTCGACGCCGTCCGCCTCGCCGCAACGCGAAACCTGCTCGTC
GCCGTCCGCGGCGGCGGCCACAGCATCGCCGGGACCTCCACAGCCGACGACAGCTTGATGATCGACCTGTCGGCGATGCG
GGGCGTCTGGGTCGATCCGGAACAGCGGCGTGTGCGGGTGGCCGGTGGTGCGACCTGGGGCGACGTCGACCGTGAGACGC
AGCTGTATGGGCTCGCGGTACCCGGGGGAGTGGTGTCGACCACGGGTGTGGCTGGTCTGACGCTCGGTGGCGGAATCGGC
TGGTTGCACCGCAAGTACGGGCTGGCCTGCGATGCGCTGCGTGCGGCCGAGGTCGTGACGGCCAGCGGCGACGTCGTGCG
GTGTAGCGCCTCCGAGCGCGAGGACCTGTTCTGGGCGCTGCGTGGCGGGGGCGGCAACTTCGGTGTGGTGGTGTCCTTCG
AGTTCGAGGCCTATCCGCTCGGTCCGGTCGTCTGGAACAGCATGGTCGTCTACCCGATCGACGCCGCTGCCGAGTTGCTC
CCCCGCTGGCGGGACTGGACGTCCACCGTCCCCGACGAGGTCACCAGCCGTGCCATGCTGTGGTCGCTGCCGGCGGTGCC
GGCACTCCCGCCCGCCGTACACAACCGCGACGTGTTCATCACAGCCGCCTTGTACGCGGGCGACCCGGACGAGGGTCAGC
GGGCGTGTCGGGCGCTTGCCGAGTTCGGCGCGCCGCTCGCCGACATGAGCCAGGCCCTGTCGTACCGGACAGCGCAGTCC
TCCCTCGACCCGTTCTTCCCGAAGGGCGGGCTGCAGAGCTACTGGAAGTCCGTCTACCTGGACCGACTCGACGAGGATGC
GACCGCGTTCGTGGTCCGCGTCGGCCAGGACCGCCCGCACCCGATGACCTTGGTGCACGTGCCTCTACTGGGAGGCGCCA
TGTCGCGGGTCGGGGCGACGGAAACCGCGTTCGGCGATCGCAGCGCCCGGTACATGCTGAGCCTCGACGGAAACTGGCTG
GACCCGGCCGACGACGACGCGAACATCCGTTGGGTGCGCGATGCCTACGACGAGGCTGTCACGCTGCGGGCCGCGTCGGG
CACCTACCTCAACTTCGGCGGCGACGCCGATCTCGACGACGCCGCCCGGGCGCGAGCCTGGGGACGCAACGTCGAGCGCC
TTCGACAGGTCAAGCGCAGCTACGACCCGGAAAACCGCTTCCGGCTCAACCCCAACATCCCCCCCGCTGAGAGCTGA

Upstream 100 bases:

>100_bases
ATGCTTTGCGATGACCTGGTAGTAGCGCGACCCTGTAGGGGTAAGTGGCGCAGACCGGCCCACCCGATGGACACCGGCGA
GCACAAGGGGAGGAGCGCTC

Downstream 100 bases:

>100_bases
GCCGACGCTTTGTGGCTGACAGCGGCCCCGGTCATACGGCGTCCGCGGCTGCGAGCCAGACCTATCCCCGACCACCACGA
CGGGTAGCGGCAATGGCCGA

Product: FAD linked oxidase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSAQVLTLEGQRQRLSDETVEEIRAIFHGQVLTSDDVGYDDVRVIQNAMLDRRPGLIIRCTGAADVVDAVRLAATRNLLV
AVRGGGHSIAGTSTADDSLMIDLSAMRGVWVDPEQRRVRVAGGATWGDVDRETQLYGLAVPGGVVSTTGVAGLTLGGGIG
WLHRKYGLACDALRAAEVVTASGDVVRCSASEREDLFWALRGGGGNFGVVVSFEFEAYPLGPVVWNSMVVYPIDAAAELL
PRWRDWTSTVPDEVTSRAMLWSLPAVPALPPAVHNRDVFITAALYAGDPDEGQRACRALAEFGAPLADMSQALSYRTAQS
SLDPFFPKGGLQSYWKSVYLDRLDEDATAFVVRVGQDRPHPMTLVHVPLLGGAMSRVGATETAFGDRSARYMLSLDGNWL
DPADDDANIRWVRDAYDEAVTLRAASGTYLNFGGDADLDDAARARAWGRNVERLRQVKRSYDPENRFRLNPNIPPAES

Sequences:

>Translated_478_residues
MSAQVLTLEGQRQRLSDETVEEIRAIFHGQVLTSDDVGYDDVRVIQNAMLDRRPGLIIRCTGAADVVDAVRLAATRNLLV
AVRGGGHSIAGTSTADDSLMIDLSAMRGVWVDPEQRRVRVAGGATWGDVDRETQLYGLAVPGGVVSTTGVAGLTLGGGIG
WLHRKYGLACDALRAAEVVTASGDVVRCSASEREDLFWALRGGGGNFGVVVSFEFEAYPLGPVVWNSMVVYPIDAAAELL
PRWRDWTSTVPDEVTSRAMLWSLPAVPALPPAVHNRDVFITAALYAGDPDEGQRACRALAEFGAPLADMSQALSYRTAQS
SLDPFFPKGGLQSYWKSVYLDRLDEDATAFVVRVGQDRPHPMTLVHVPLLGGAMSRVGATETAFGDRSARYMLSLDGNWL
DPADDDANIRWVRDAYDEAVTLRAASGTYLNFGGDADLDDAARARAWGRNVERLRQVKRSYDPENRFRLNPNIPPAES
>Mature_477_residues
SAQVLTLEGQRQRLSDETVEEIRAIFHGQVLTSDDVGYDDVRVIQNAMLDRRPGLIIRCTGAADVVDAVRLAATRNLLVA
VRGGGHSIAGTSTADDSLMIDLSAMRGVWVDPEQRRVRVAGGATWGDVDRETQLYGLAVPGGVVSTTGVAGLTLGGGIGW
LHRKYGLACDALRAAEVVTASGDVVRCSASEREDLFWALRGGGGNFGVVVSFEFEAYPLGPVVWNSMVVYPIDAAAELLP
RWRDWTSTVPDEVTSRAMLWSLPAVPALPPAVHNRDVFITAALYAGDPDEGQRACRALAEFGAPLADMSQALSYRTAQSS
LDPFFPKGGLQSYWKSVYLDRLDEDATAFVVRVGQDRPHPMTLVHVPLLGGAMSRVGATETAFGDRSARYMLSLDGNWLD
PADDDANIRWVRDAYDEAVTLRAASGTYLNFGGDADLDDAARARAWGRNVERLRQVKRSYDPENRFRLNPNIPPAES

Specific function: Involved in mitomycin resistance; oxidizes reduced form of mitomycins [H]

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012951
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR006093 [H]

Pfam domain/function: PF08031 BBE; PF01565 FAD_binding_4 [H]

EC number: NA

Molecular weight: Translated: 51866; Mature: 51735

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00862 OX2_COVAL_FAD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQVLTLEGQRQRLSDETVEEIRAIFHGQVLTSDDVGYDDVRVIQNAMLDRRPGLIIRC
CCCEEEEECCCHHHCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCEEEEE
TGAADVVDAVRLAATRNLLVAVRGGGHSIAGTSTADDSLMIDLSAMRGVWVDPEQRRVRV
CCCHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCEEEEEHHHCCCCCCCHHCEEEE
AGGATWGDVDRETQLYGLAVPGGVVSTTGVAGLTLGGGIGWLHRKYGLACDALRAAEVVT
ECCCCCCCCCCCCEEEEEECCCCCEECCCCCEEEECCCHHHHHHHHCCHHHHHHHHHEEE
ASGDVVRCSASEREDLFWALRGGGGNFGVVVSFEFEAYPLGPVVWNSMVVYPIDAAAELL
CCCCEEEECCCCCCCEEEEEECCCCCEEEEEEEEECCEECCHHHHCCEEEEECCHHHHHH
PRWRDWTSTVPDEVTSRAMLWSLPAVPALPPAVHNRDVFITAALYAGDPDEGQRACRALA
HHHHCCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
EFGAPLADMSQALSYRTAQSSLDPFFPKGGLQSYWKSVYLDRLDEDATAFVVRVGQDRPH
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
PMTLVHVPLLGGAMSRVGATETAFGDRSARYMLSLDGNWLDPADDDANIRWVRDAYDEAV
CEEEEEECHHCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHCCCCEE
TLRAASGTYLNFGGDADLDDAARARAWGRNVERLRQVKRSYDPENRFRLNPNIPPAES
EEEECCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
SAQVLTLEGQRQRLSDETVEEIRAIFHGQVLTSDDVGYDDVRVIQNAMLDRRPGLIIRC
CCEEEEECCCHHHCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCEEEEE
TGAADVVDAVRLAATRNLLVAVRGGGHSIAGTSTADDSLMIDLSAMRGVWVDPEQRRVRV
CCCHHHHHHHHHHHCCCEEEEEECCCCEECCCCCCCCCEEEEEHHHCCCCCCCHHCEEEE
AGGATWGDVDRETQLYGLAVPGGVVSTTGVAGLTLGGGIGWLHRKYGLACDALRAAEVVT
ECCCCCCCCCCCCEEEEEECCCCCEECCCCCEEEECCCHHHHHHHHCCHHHHHHHHHEEE
ASGDVVRCSASEREDLFWALRGGGGNFGVVVSFEFEAYPLGPVVWNSMVVYPIDAAAELL
CCCCEEEECCCCCCCEEEEEECCCCCEEEEEEEEECCEECCHHHHCCEEEEECCHHHHHH
PRWRDWTSTVPDEVTSRAMLWSLPAVPALPPAVHNRDVFITAALYAGDPDEGQRACRALA
HHHHCCCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
EFGAPLADMSQALSYRTAQSSLDPFFPKGGLQSYWKSVYLDRLDEDATAFVVRVGQDRPH
HHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
PMTLVHVPLLGGAMSRVGATETAFGDRSARYMLSLDGNWLDPADDDANIRWVRDAYDEAV
CEEEEEECHHCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEHHCCCCEE
TLRAASGTYLNFGGDADLDDAARARAWGRNVERLRQVKRSYDPENRFRLNPNIPPAES
EEEECCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517396 [H]