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The map label for this gene is ppsA [H]

Identifier: 158317128

GI number: 158317128

Start: 6476186

End: 6477502

Strand: Reverse

Name: ppsA [H]

Synonym: Franean1_5372

Alternate gene names: 158317128

Gene position: 6477502-6476186 (Counterclockwise)

Preceding gene: 158317129

Following gene: 158317126

Centisome position: 72.12

GC content: 73.88

Gene sequence:

>1317_bases
ATGGCAGTTGAGCGTCCCGGGGAGAGGCGGGAGGGCTCGGTCACCGTCGAAGGACGGGAGAGCGCGGTTGCCGTCATCGG
CATGGGCTGTGAGCTGCCGTCCGCCCGTGGTCCGCGGGAGCTGTGGCGTCTGCTGGCCGAGGCGCGCGACGCGGTCGGGC
CGGCCCGGGCCGGGACCGGTCTGCGGCGGGCCGGGCACATCGACGGCGCCGGCTGTTCCGACCTGACCCGCTTCGGTATC
GATCCCGACGAGGCGGCCCGGATGGATCCCCAGCAGCACGTGCTGCTGCGGGTCGTGCACGACGCCATCGCGGACGCCGG
CCTCGACCCGGCCGGCATCGCCGGGAGCCCCACAGCCGTCTGTGTCGGGCAGTCCGCCTCGGACTTCTGGGATCTCCGGT
CCCGGTCCGCCGGGCTCGACCTGGGTGACTTCGTGGGCTCCCACCAGCGCGGCCTTCTCTCCGGGCGGGTGAGTCACACG
TTCGATCTCCGAGGGCCCTCCGTCACTGTGGACGCGGCGCAGGCGTCCTCCCTGGTGGCGGTCCACCTGGCCTGCCGCAG
CCTGCGCGCGGGGGACGCGGAGCTGGCCCTTGCCGGCGGCGTCAGCCTGCTGTCGGGCCGGCAGGCCGGTGTCATGCTCG
GTCGCGGCGGGGTGCTCTCCCCCGACGGGCGGTGCAAGTTCGGCGACGTGTCCGCCGACGGCTTCGTGCGGGCCGAGGGG
GCCGGCGTGGTGCTGCTCAAGCCGCTGGCCCGCGCGCTGTTGGACGGCGACCGTGTGCGCGCCGTCATCGCCGGCAGCGC
AGTCACCAATGACGGCCGGGACAAGGAGACGCTGCTGCACCCCTCGGAGAAGGGCCAGGCCGAAGCGATGCGTCGTGCCT
ACCTGGACGCCGGGATCGCGCCCGCCGAGGTCGACTATGTCGAGGCGCACGGCACCGGCACCCGGATCGACGTGGCGGAG
CTGCGCGCGCTCACCGCCGTGCTGGGGCAGGGGCGCCCGGCCGGCCGACCCTGCCTGGTCGGCTCGCTCAAGTCCAACAT
TGGCCACGCCGAGGCCGCGGGCGGCGTCGCCGGGCTCATCAAGACCGTGCTGTGCCTGGAGCACGGCGCCGTTCCCGCCA
GCCTCCACTTCACCACCCCCCACCCGGCGGTCGACTGGGACCGTGTTCCCCTCGCCGTGCCGACCGTCCTGACCAGGCTG
CCGGACCGGGGAACGCCCGTCGTCGCGGCGGTCAACGGCCAGGGTGTTTCCTCGACCAACGTTCACGTGGTACTCACGAC
GACCCCGGAAAAAAGGGCGCCCGGCGGGCGACCGTAA

Upstream 100 bases:

>100_bases
CTCACCCCGATCAACGAGTGGGTCGCTGCCCAGCCCTGGAAAATATGGGCGGCCGCCCGCTCGAACGGCGCCGGGACGCC
GGATCCGGTCAGAGCCGACC

Downstream 100 bases:

>100_bases
TCCGGGCGTCGGCAGGTGCGGGCCTTTCCATGCCACTTTCCTGGCTCGCCTCGCTGCTGTTCCCCAGAACATCCTGGAGG
CAACGATGTTTTTCCGTGGC

Product: mycocerosate synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAVERPGERREGSVTVEGRESAVAVIGMGCELPSARGPRELWRLLAEARDAVGPARAGTGLRRAGHIDGAGCSDLTRFGI
DPDEAARMDPQQHVLLRVVHDAIADAGLDPAGIAGSPTAVCVGQSASDFWDLRSRSAGLDLGDFVGSHQRGLLSGRVSHT
FDLRGPSVTVDAAQASSLVAVHLACRSLRAGDAELALAGGVSLLSGRQAGVMLGRGGVLSPDGRCKFGDVSADGFVRAEG
AGVVLLKPLARALLDGDRVRAVIAGSAVTNDGRDKETLLHPSEKGQAEAMRRAYLDAGIAPAEVDYVEAHGTGTRIDVAE
LRALTAVLGQGRPAGRPCLVGSLKSNIGHAEAAGGVAGLIKTVLCLEHGAVPASLHFTTPHPAVDWDRVPLAVPTVLTRL
PDRGTPVVAAVNGQGVSSTNVHVVLTTTPEKRAPGGRP

Sequences:

>Translated_438_residues
MAVERPGERREGSVTVEGRESAVAVIGMGCELPSARGPRELWRLLAEARDAVGPARAGTGLRRAGHIDGAGCSDLTRFGI
DPDEAARMDPQQHVLLRVVHDAIADAGLDPAGIAGSPTAVCVGQSASDFWDLRSRSAGLDLGDFVGSHQRGLLSGRVSHT
FDLRGPSVTVDAAQASSLVAVHLACRSLRAGDAELALAGGVSLLSGRQAGVMLGRGGVLSPDGRCKFGDVSADGFVRAEG
AGVVLLKPLARALLDGDRVRAVIAGSAVTNDGRDKETLLHPSEKGQAEAMRRAYLDAGIAPAEVDYVEAHGTGTRIDVAE
LRALTAVLGQGRPAGRPCLVGSLKSNIGHAEAAGGVAGLIKTVLCLEHGAVPASLHFTTPHPAVDWDRVPLAVPTVLTRL
PDRGTPVVAAVNGQGVSSTNVHVVLTTTPEKRAPGGRP
>Mature_437_residues
AVERPGERREGSVTVEGRESAVAVIGMGCELPSARGPRELWRLLAEARDAVGPARAGTGLRRAGHIDGAGCSDLTRFGID
PDEAARMDPQQHVLLRVVHDAIADAGLDPAGIAGSPTAVCVGQSASDFWDLRSRSAGLDLGDFVGSHQRGLLSGRVSHTF
DLRGPSVTVDAAQASSLVAVHLACRSLRAGDAELALAGGVSLLSGRQAGVMLGRGGVLSPDGRCKFGDVSADGFVRAEGA
GVVLLKPLARALLDGDRVRAVIAGSAVTNDGRDKETLLHPSEKGQAEAMRRAYLDAGIAPAEVDYVEAHGTGTRIDVAEL
RALTAVLGQGRPAGRPCLVGSLKSNIGHAEAAGGVAGLIKTVLCLEHGAVPASLHFTTPHPAVDWDRVPLAVPTVLTRLP
DRGTPVVAAVNGQGVSSTNVHVVLTTTPEKRAPGGRP

Specific function: Polyketide synthase involved in phenolpthiocerol synthesis [H]

COG id: COG3321

COG function: function code Q; Polyketide synthase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=412, Percent_Identity=36.8932038834951, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI8923559, Length=253, Percent_Identity=28.8537549407115, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1787337, Length=260, Percent_Identity=31.9230769230769, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788663, Length=271, Percent_Identity=29.1512915129151, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI212642053, Length=377, Percent_Identity=35.0132625994695, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17550940, Length=433, Percent_Identity=28.6374133949192, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983541, Length=238, Percent_Identity=30.672268907563, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983535, Length=219, Percent_Identity=31.0502283105023, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320904, Length=391, Percent_Identity=24.2966751918159, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221330659, Length=420, Percent_Identity=33.5714285714286, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI19920632, Length=420, Percent_Identity=33.5714285714286, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24581345, Length=454, Percent_Identity=32.8193832599119, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24644341, Length=329, Percent_Identity=30.3951367781155, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR000794
- InterPro:   IPR002198
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020842
- InterPro:   IPR020801
- InterPro:   IPR020841
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF00106 adh_short; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 45043; Mature: 44911

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVERPGERREGSVTVEGRESAVAVIGMGCELPSARGPRELWRLLAEARDAVGPARAGTG
CCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCC
LRRAGHIDGAGCSDLTRFGIDPDEAARMDPQQHVLLRVVHDAIADAGLDPAGIAGSPTAV
CHHCCCCCCCCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
CVGQSASDFWDLRSRSAGLDLGDFVGSHQRGLLSGRVSHTFDLRGPSVTVDAAQASSLVA
EECCCCHHHHHHHHCCCCCCHHHHHCCCCCCHHCCCCCEEEECCCCEEEEEHHHHHHHHH
VHLACRSLRAGDAELALAGGVSLLSGRQAGVMLGRGGVLSPDGRCKFGDVSADGFVRAEG
HHHHHHHCCCCCCEEEEECCHHHHCCCCCCEEEECCCCCCCCCCEEECCCCCCCEEEECC
AGVVLLKPLARALLDGDRVRAVIAGSAVTNDGRDKETLLHPSEKGQAEAMRRAYLDAGIA
CCEEEHHHHHHHHHCCCCEEEEEECCEECCCCCCCHHEECCCCCCHHHHHHHHHHHCCCC
PAEVDYVEAHGTGTRIDVAELRALTAVLGQGRPAGRPCLVGSLKSNIGHAEAAGGVAGLI
CCEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCHHHHCHHHHHH
KTVLCLEHGAVPASLHFTTPHPAVDWDRVPLAVPTVLTRLPDRGTPVVAAVNGQGVSSTN
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VHVVLTTTPEKRAPGGRP
EEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
AVERPGERREGSVTVEGRESAVAVIGMGCELPSARGPRELWRLLAEARDAVGPARAGTG
CCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCC
LRRAGHIDGAGCSDLTRFGIDPDEAARMDPQQHVLLRVVHDAIADAGLDPAGIAGSPTAV
CHHCCCCCCCCCHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEE
CVGQSASDFWDLRSRSAGLDLGDFVGSHQRGLLSGRVSHTFDLRGPSVTVDAAQASSLVA
EECCCCHHHHHHHHCCCCCCHHHHHCCCCCCHHCCCCCEEEECCCCEEEEEHHHHHHHHH
VHLACRSLRAGDAELALAGGVSLLSGRQAGVMLGRGGVLSPDGRCKFGDVSADGFVRAEG
HHHHHHHCCCCCCEEEEECCHHHHCCCCCCEEEECCCCCCCCCCEEECCCCCCCEEEECC
AGVVLLKPLARALLDGDRVRAVIAGSAVTNDGRDKETLLHPSEKGQAEAMRRAYLDAGIA
CCEEEHHHHHHHHHCCCCEEEEEECCEECCCCCCCHHEECCCCCCHHHHHHHHHHHCCCC
PAEVDYVEAHGTGTRIDVAELRALTAVLGQGRPAGRPCLVGSLKSNIGHAEAAGGVAGLI
CCEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCHHHHCHHHHHH
KTVLCLEHGAVPASLHFTTPHPAVDWDRVPLAVPTVLTRLPDRGTPVVAAVNGQGVSSTN
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VHVVLTTTPEKRAPGGRP
EEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]