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The map label for this gene is xthA [H]

Identifier: 158317046

GI number: 158317046

Start: 6363858

End: 6364751

Strand: Direct

Name: xthA [H]

Synonym: Franean1_5290

Alternate gene names: 158317046

Gene position: 6363858-6364751 (Clockwise)

Preceding gene: 158317041

Following gene: 158317051

Centisome position: 70.85

GC content: 73.6

Gene sequence:

>894_bases
GTGCGGATCGCGACCTGGAACATCAACTCGGCCAAGGCCCGGCAGCCCCGGCTGATCGAATGGCTTGACCGCGCGAGCCC
CGACGTGGTCTGCCTGCAGGAGACCAAGCTCTCGGACGAGGCGTTCCTCGAGCTCTTCGACGAAGATCTCTTCCGCCGCG
GCTACCGGGTCGCGCACCACGGCGACGGCCGCTGGAACGGGGTGGCGATCCTCTCCCGCGCCGGGCTGGAGGATGTGCGG
CGCGGGCTGCCCGGCGACCCCGGGTTCCCGGGGCCCGAGCCGCGGGCGATCTCCGCGAGCTGCGGCGGGATCCGGATCTG
GTCGCTGTACGTGCCCAACGGGCGCACGGTGGAGGACCCGCACTACGCCTACAAGCTGGCCTGGCTGGCCGCACTGCGCG
ACGTCGTGGCCGAACTCGCCCCGCAGGGGCCGGTGATGACGCTCGGCGACTTCAACATCGCGCCGACGGACGCCGACGTC
TGGGACATCGCCGAGTTCGAGGGCGCGACCCACGTGACCGAGGCCGAGCGCGCCGCGCTCGGGCGGCTGCACGACGCCGG
CCTGCTCGACCTGATGCGCGAGCGCTGGCCGGACGAGACCGTCTACACCTACTGGGACTACCGCCAGCTCTGCTTCCCCA
AGAACTACGGGATGCGCATCGACCTGACCCTGGCCACGGCCGACGTCGCGGCCCGGGCACGCGCGGTGTGGGTAGACCGG
GCTGCCCGCAAGGGCACCGGGACGAGCGACCACGCCCCGGTCGTCGTCGACCTGGACGAGGCGCCGGACGGCGACATCGG
CCCGATGGTGCCGCCCCCGTCCTCGCGCGGCTCCTCCGCGCGTGGGTCGTCCTCCCGCGCCGGGGCGGGCACCCGCCGCC
GCGCCTCCACCTGA

Upstream 100 bases:

>100_bases
ACGCTCCTGCCAGGAGCGGACATCGGTGTCATGTCTCCGAGGATGGCACGGACCGGTCGGTGCGGAGATCTGTCGGAGGT
GAGGACTACCGTCTCATGGT

Downstream 100 bases:

>100_bases
CCGCTCCCCCCGGCCGGCTGCCGACGCCGGCTCGGCCCGTAACGCAGTCGCCCCCGGCGAACGGACCAGACAGGGTCCGC
ACACCGGGGGCGATCATGCT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRIATWNINSAKARQPRLIEWLDRASPDVVCLQETKLSDEAFLELFDEDLFRRGYRVAHHGDGRWNGVAILSRAGLEDVR
RGLPGDPGFPGPEPRAISASCGGIRIWSLYVPNGRTVEDPHYAYKLAWLAALRDVVAELAPQGPVMTLGDFNIAPTDADV
WDIAEFEGATHVTEAERAALGRLHDAGLLDLMRERWPDETVYTYWDYRQLCFPKNYGMRIDLTLATADVAARARAVWVDR
AARKGTGTSDHAPVVVDLDEAPDGDIGPMVPPPSSRGSSARGSSSRAGAGTRRRAST

Sequences:

>Translated_297_residues
MRIATWNINSAKARQPRLIEWLDRASPDVVCLQETKLSDEAFLELFDEDLFRRGYRVAHHGDGRWNGVAILSRAGLEDVR
RGLPGDPGFPGPEPRAISASCGGIRIWSLYVPNGRTVEDPHYAYKLAWLAALRDVVAELAPQGPVMTLGDFNIAPTDADV
WDIAEFEGATHVTEAERAALGRLHDAGLLDLMRERWPDETVYTYWDYRQLCFPKNYGMRIDLTLATADVAARARAVWVDR
AARKGTGTSDHAPVVVDLDEAPDGDIGPMVPPPSSRGSSARGSSSRAGAGTRRRAST
>Mature_297_residues
MRIATWNINSAKARQPRLIEWLDRASPDVVCLQETKLSDEAFLELFDEDLFRRGYRVAHHGDGRWNGVAILSRAGLEDVR
RGLPGDPGFPGPEPRAISASCGGIRIWSLYVPNGRTVEDPHYAYKLAWLAALRDVVAELAPQGPVMTLGDFNIAPTDADV
WDIAEFEGATHVTEAERAALGRLHDAGLLDLMRERWPDETVYTYWDYRQLCFPKNYGMRIDLTLATADVAARARAVWVDR
AARKGTGTSDHAPVVVDLDEAPDGDIGPMVPPPSSRGSSARGSSSRAGAGTRRRAST

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=28.8973384030418, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=28.8973384030418, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=28.8973384030418, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=33.2089552238806, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=25.8555133079848, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=26.8199233716475, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32649; Mature: 32649

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNINSAKARQPRLIEWLDRASPDVVCLQETKLSDEAFLELFDEDLFRRGYRVAHH
CEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEC
GDGRWNGVAILSRAGLEDVRRGLPGDPGFPGPEPRAISASCGGIRIWSLYVPNGRTVEDP
CCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCEECCC
HYAYKLAWLAALRDVVAELAPQGPVMTLGDFNIAPTDADVWDIAEFEGATHVTEAERAAL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
GRLHDAGLLDLMRERWPDETVYTYWDYRQLCFPKNYGMRIDLTLATADVAARARAVWVDR
HHHCCCHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHH
AARKGTGTSDHAPVVVDLDEAPDGDIGPMVPPPSSRGSSARGSSSRAGAGTRRRAST
HHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIATWNINSAKARQPRLIEWLDRASPDVVCLQETKLSDEAFLELFDEDLFRRGYRVAHH
CEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEC
GDGRWNGVAILSRAGLEDVRRGLPGDPGFPGPEPRAISASCGGIRIWSLYVPNGRTVEDP
CCCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCEECCC
HYAYKLAWLAALRDVVAELAPQGPVMTLGDFNIAPTDADVWDIAEFEGATHVTEAERAAL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
GRLHDAGLLDLMRERWPDETVYTYWDYRQLCFPKNYGMRIDLTLATADVAARARAVWVDR
HHHCCCHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHH
AARKGTGTSDHAPVVVDLDEAPDGDIGPMVPPPSSRGSSARGSSSRAGAGTRRRAST
HHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]