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The map label for this gene is tyrS [H]

Identifier: 158316978

GI number: 158316978

Start: 6270228

End: 6271625

Strand: Reverse

Name: tyrS [H]

Synonym: Franean1_5222

Alternate gene names: 158316978

Gene position: 6271625-6270228 (Counterclockwise)

Preceding gene: 158316979

Following gene: 158316976

Centisome position: 69.82

GC content: 71.53

Gene sequence:

>1398_bases
ATGACCCGACTCGGAGCCTCCGTCGACCATGTCACCGCGCTGCTCGGCGGGGCCGACCTGGCGTCCGACGCCTCCGTGCG
TGAGCTGCTGGAGCAGACCACCGAACGTCGCGGCCTCGACCTCTCCGACCTCGCCCCCGCCGAGCAGGCGGCCCTGATCG
CCGAGCGCTCGCAGGACCTGCAGCCCTCACCGGAGGCGCTGGCGGCCCGCATCGGCGACGCGGCGGCCCGGTCGCGTCCC
TTCGTGGCGAAGTTCGGCATCGACCCGACCGGCGCCGAGGTCCACCTCGGCCACGCGGTGCCGATGCTGCTCCTGAGCCG
GTTCGCGCGGATGGGGCACCGCGTCGTGTTCATCGTCGGTGACATCACCGCCAAGATCGGCGACCCGTCCGGCCGGTCGG
ACGAGCGCCCGGCGCTCACCGACGAGGACATCGCCCGCAACCTGACCACCTACCGGGCCCAGGTCACCCCGTTCTTCGAC
TTCGAACGGGCCGAGTTCCGCCGCAACGGCGACTGGCTGCGCGAGGTGAGGCTGCCGCGGCTGCTGGAGATCGTCTCGCG
CATCCCGGTGTCGATGTCGCTGCAGCGGGAGGACTTCCGGCGTCGCCTCGACGCCGGCCACGGGCTCTCCCTGGCCGAGC
TGCTGTACTCGGTGGTGATGGCGCTGGACTCGGTCGAGGTCGACAGCGACCTGGAGATCGGTGGCATCGACCAGTTCCTG
AACATGCAGATGTGCCGCAAGGTCATGGAGATCTGTGGCCAGACCCCGGAGATCGTCGTGGCCACCTCGCTGATCGAGGG
GACCGACGGCACCGGCGCCAAGATGAGCAAGAGCCGCGGCAACTACGTCCCGCTGACGGCCGCGCCCGGTGAGATCTTCG
GGAAGATCATGTCCGTGCCGGACAGGCTCGTCGAGCCGTACTTCCGCGCGCTCAGCGAGTGGCGCGACTCCGAACTCGCC
GTCCTGGCCGCCCGGCGCGAGGCCGGCACGGCGCACCCGATGGACCTCAAGAAGATCCTCGCCGGCGAGGTGACGGCGGC
GATCCACGGGGTGGACGCCGCGATGACGGCCCGGGCCGAGTTCGTCGCGCGGTTCTCCCGCCGCACCTTCGCCGAGGTCG
ACGACCTGCCGGTGCTCTCCGATCTCGGCGCAACCGTCGCCGACGCGGTGCGGGGGCTCGGGTTCGCGAAGAGCAACGGC
GACGTCCGCCGGGTCGCGGAGCAGAACGGGCTGCGGCTGGTGGTCGAGTCCGAGGAGGGCCAGACCCAGGTGCCGCTCAC
GCCGGACGACGTCCGGGAGCCGCTGTCCGCCGTCCTCAAGGACAGGCTCGACGGCCGGCCCGGTGCCGTGTATCTCAAGG
TCGGCCGCAAGCTGGCGCGGATCGAGCCTGCCGGCTGA

Upstream 100 bases:

>100_bases
CGCGGCCGCGCCGCGGCCCGCCGCCGCACGGCCCGCCGCCTGACGGGGAGGGTGGTCCGCGCCGAGATCAGGGCGCTCGG
CGCTGACTACCATTGTCGCC

Downstream 100 bases:

>100_bases
CGGCACCCGCCGCGCGGGCACCGGTCGCACGGCGTCGGTGTCAGAGGTCCTGAGCCTCCGTGCGGGCGGTCTCCGCCGGC
GGTGAGCCGGGGTGCGCCGC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTRLGASVDHVTALLGGADLASDASVRELLEQTTERRGLDLSDLAPAEQAALIAERSQDLQPSPEALAARIGDAAARSRP
FVAKFGIDPTGAEVHLGHAVPMLLLSRFARMGHRVVFIVGDITAKIGDPSGRSDERPALTDEDIARNLTTYRAQVTPFFD
FERAEFRRNGDWLREVRLPRLLEIVSRIPVSMSLQREDFRRRLDAGHGLSLAELLYSVVMALDSVEVDSDLEIGGIDQFL
NMQMCRKVMEICGQTPEIVVATSLIEGTDGTGAKMSKSRGNYVPLTAAPGEIFGKIMSVPDRLVEPYFRALSEWRDSELA
VLAARREAGTAHPMDLKKILAGEVTAAIHGVDAAMTARAEFVARFSRRTFAEVDDLPVLSDLGATVADAVRGLGFAKSNG
DVRRVAEQNGLRLVVESEEGQTQVPLTPDDVREPLSAVLKDRLDGRPGAVYLKVGRKLARIEPAG

Sequences:

>Translated_465_residues
MTRLGASVDHVTALLGGADLASDASVRELLEQTTERRGLDLSDLAPAEQAALIAERSQDLQPSPEALAARIGDAAARSRP
FVAKFGIDPTGAEVHLGHAVPMLLLSRFARMGHRVVFIVGDITAKIGDPSGRSDERPALTDEDIARNLTTYRAQVTPFFD
FERAEFRRNGDWLREVRLPRLLEIVSRIPVSMSLQREDFRRRLDAGHGLSLAELLYSVVMALDSVEVDSDLEIGGIDQFL
NMQMCRKVMEICGQTPEIVVATSLIEGTDGTGAKMSKSRGNYVPLTAAPGEIFGKIMSVPDRLVEPYFRALSEWRDSELA
VLAARREAGTAHPMDLKKILAGEVTAAIHGVDAAMTARAEFVARFSRRTFAEVDDLPVLSDLGATVADAVRGLGFAKSNG
DVRRVAEQNGLRLVVESEEGQTQVPLTPDDVREPLSAVLKDRLDGRPGAVYLKVGRKLARIEPAG
>Mature_464_residues
TRLGASVDHVTALLGGADLASDASVRELLEQTTERRGLDLSDLAPAEQAALIAERSQDLQPSPEALAARIGDAAARSRPF
VAKFGIDPTGAEVHLGHAVPMLLLSRFARMGHRVVFIVGDITAKIGDPSGRSDERPALTDEDIARNLTTYRAQVTPFFDF
ERAEFRRNGDWLREVRLPRLLEIVSRIPVSMSLQREDFRRRLDAGHGLSLAELLYSVVMALDSVEVDSDLEIGGIDQFLN
MQMCRKVMEICGQTPEIVVATSLIEGTDGTGAKMSKSRGNYVPLTAAPGEIFGKIMSVPDRLVEPYFRALSEWRDSELAV
LAARREAGTAHPMDLKKILAGEVTAAIHGVDAAMTARAEFVARFSRRTFAEVDDLPVLSDLGATVADAVRGLGFAKSNGD
VRRVAEQNGLRLVVESEEGQTQVPLTPDDVREPLSAVLKDRLDGRPGAVYLKVGRKLARIEPAG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=338, Percent_Identity=28.9940828402367, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1787925, Length=307, Percent_Identity=28.9902280130293, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25145802, Length=334, Percent_Identity=25.748502994012, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=305, Percent_Identity=26.2295081967213, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI20130383, Length=310, Percent_Identity=24.8387096774194, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 50539; Mature: 50408

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLGASVDHVTALLGGADLASDASVRELLEQTTERRGLDLSDLAPAEQAALIAERSQDL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCC
QPSPEALAARIGDAAARSRPFVAKFGIDPTGAEVHLGHAVPMLLLSRFARMGHRVVFIVG
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEEEE
DITAKIGDPSGRSDERPALTDEDIARNLTTYRAQVTPFFDFERAEFRRNGDWLREVRLPR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHCCCHHHHHHHHHH
LLEIVSRIPVSMSLQREDFRRRLDAGHGLSLAELLYSVVMALDSVEVDSDLEIGGIDQFL
HHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
NMQMCRKVMEICGQTPEIVVATSLIEGTDGTGAKMSKSRGNYVPLTAAPGEIFGKIMSVP
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCH
DRLVEPYFRALSEWRDSELAVLAARREAGTAHPMDLKKILAGEVTAAIHGVDAAMTARAE
HHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
FVARFSRRTFAEVDDLPVLSDLGATVADAVRGLGFAKSNGDVRRVAEQNGLRLVVESEEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCC
QTQVPLTPDDVREPLSAVLKDRLDGRPGAVYLKVGRKLARIEPAG
CEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCC
>Mature Secondary Structure 
TRLGASVDHVTALLGGADLASDASVRELLEQTTERRGLDLSDLAPAEQAALIAERSQDL
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHCCCC
QPSPEALAARIGDAAARSRPFVAKFGIDPTGAEVHLGHAVPMLLLSRFARMGHRVVFIVG
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECHHHHHHHHHHHHHCCCEEEEEEE
DITAKIGDPSGRSDERPALTDEDIARNLTTYRAQVTPFFDFERAEFRRNGDWLREVRLPR
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCHHHHHHCCCHHHHHHHHHH
LLEIVSRIPVSMSLQREDFRRRLDAGHGLSLAELLYSVVMALDSVEVDSDLEIGGIDQFL
HHHHHHHCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
NMQMCRKVMEICGQTPEIVVATSLIEGTDGTGAKMSKSRGNYVPLTAAPGEIFGKIMSVP
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCH
DRLVEPYFRALSEWRDSELAVLAARREAGTAHPMDLKKILAGEVTAAIHGVDAAMTARAE
HHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
FVARFSRRTFAEVDDLPVLSDLGATVADAVRGLGFAKSNGDVRRVAEQNGLRLVVESEEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECCCC
QTQVPLTPDDVREPLSAVLKDRLDGRPGAVYLKVGRKLARIEPAG
CEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]