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The map label for this gene is xylF [H]

Identifier: 158316943

GI number: 158316943

Start: 6227480

End: 6228637

Strand: Reverse

Name: xylF [H]

Synonym: Franean1_5187

Alternate gene names: 158316943

Gene position: 6228637-6227480 (Counterclockwise)

Preceding gene: 158316944

Following gene: 158316942

Centisome position: 69.35

GC content: 70.03

Gene sequence:

>1158_bases
GTGGCAGACAACTCGCTCGACGACGGGGTACCTCGGCGCGGTCGGATGCGCGGCCATGCGGGGAGACGGCGAGGTCGCCT
TTTCGTGGCCGCGTCGGTGGCGGTCATGGTCGTGCTGGCCGCCGTGGGATGCACATCGGACTCGTCCGATGACGAGGTGC
CCCAGGGAGGATCCGAGAGCGGAACCATCGCGCTCCTGTTACCCGAGACCCAGACGACCCGCTACGAATCGGCCGACCGC
CCCTACTTCGAGGCGCGGATGGCGAAGATCTGCCCCGACTGCAAGGTGCTGTACTCGAACGCCGACCAGGACTCGGCCGC
CCAGCAGAACCAGGCCGAGCAGGCCATGACCAACGGCGCCAAGGTCCTCGTCCTGGACCCGGTGGACGGCGAGGCCGCGG
CGGTGATCGCCCGCAATGCGCGGGACCGTGGCGTGCGCGTGGTCTCCTACGACCGGCTCATCCAGAAGGCGCCCGTCGAC
GCCTACATCTCCTTCGACAATGAGAAGGTCGGCCAGTTGCAGGGCCAGGCGCTCCTCGACGCGATCGGCGACCGGGCCGG
CGCCGGCAAGGTCATCATGATCAACGGCTCGCAGGACGACCCGAACGCCCAGCAGTTCAAGGACGGCGCGCTGTCGGTCC
TGGAGGGCAAGGTGACGATCGGCTTCGACACGTTCACCCCCGACTGGTCTCCCGACACCGCCGGTCGGGAGATGGACCAG
GCGATCACCACCGTCGGCCGGGAGAACATCGTCGGGGTCTACGCCGCGAACGACGGCATGGCCGGCGCCGTGGTCGCCGC
GCTGCGCCGGGCGAACGTGAACCCGCTGCCGCCCGTCACCGGCCAGGACGCCGAACTCGCCGGGGTACAGCGCGTACTCG
CCGGAGATCAGCACATGACCGTCTACAAGGCCATCCGCCCCGAGGCGGAGCAGGCGGCCGACCTGGCGCTCGCGCTGCTG
CGCGGTGAGCCCGTCGACACGATCGCGACCGGGCACGTCGACAACGGCAACGGCCAGGTTCCCGCCGTCCTGCTGGAACC
GGTCGCGGTCACCCGGGACACCGTCGCCGCGACGGTGGTGAAGGACGGCTTCATCGCCAAGGCCGACCTGTGTGCCGGCA
CGTACGCGACAGCCTGCGCGTCCGCCGGCATCTCCTGA

Upstream 100 bases:

>100_bases
TTGTATTCAAGTCTTGACGGCAAAGTCATCCTCTCCGCAGACTGCTACCAGAGCGTGACCGGTTCGCTCCGGCACGCAGC
AGCGAACTCGGGAGGGGCGC

Downstream 100 bases:

>100_bases
CCTCGCGCCCCCGCCCCCGGGATCCGCGGTGAGCTGCTTTGCCGGCGGCTCACCGCGGATCCCCTCGTGACTGTTCTTGG
AAGGCACGGGCCCGTCATGT

Product: solute-binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MADNSLDDGVPRRGRMRGHAGRRRGRLFVAASVAVMVVLAAVGCTSDSSDDEVPQGGSESGTIALLLPETQTTRYESADR
PYFEARMAKICPDCKVLYSNADQDSAAQQNQAEQAMTNGAKVLVLDPVDGEAAAVIARNARDRGVRVVSYDRLIQKAPVD
AYISFDNEKVGQLQGQALLDAIGDRAGAGKVIMINGSQDDPNAQQFKDGALSVLEGKVTIGFDTFTPDWSPDTAGREMDQ
AITTVGRENIVGVYAANDGMAGAVVAALRRANVNPLPPVTGQDAELAGVQRVLAGDQHMTVYKAIRPEAEQAADLALALL
RGEPVDTIATGHVDNGNGQVPAVLLEPVAVTRDTVAATVVKDGFIAKADLCAGTYATACASAGIS

Sequences:

>Translated_385_residues
MADNSLDDGVPRRGRMRGHAGRRRGRLFVAASVAVMVVLAAVGCTSDSSDDEVPQGGSESGTIALLLPETQTTRYESADR
PYFEARMAKICPDCKVLYSNADQDSAAQQNQAEQAMTNGAKVLVLDPVDGEAAAVIARNARDRGVRVVSYDRLIQKAPVD
AYISFDNEKVGQLQGQALLDAIGDRAGAGKVIMINGSQDDPNAQQFKDGALSVLEGKVTIGFDTFTPDWSPDTAGREMDQ
AITTVGRENIVGVYAANDGMAGAVVAALRRANVNPLPPVTGQDAELAGVQRVLAGDQHMTVYKAIRPEAEQAADLALALL
RGEPVDTIATGHVDNGNGQVPAVLLEPVAVTRDTVAATVVKDGFIAKADLCAGTYATACASAGIS
>Mature_384_residues
ADNSLDDGVPRRGRMRGHAGRRRGRLFVAASVAVMVVLAAVGCTSDSSDDEVPQGGSESGTIALLLPETQTTRYESADRP
YFEARMAKICPDCKVLYSNADQDSAAQQNQAEQAMTNGAKVLVLDPVDGEAAAVIARNARDRGVRVVSYDRLIQKAPVDA
YISFDNEKVGQLQGQALLDAIGDRAGAGKVIMINGSQDDPNAQQFKDGALSVLEGKVTIGFDTFTPDWSPDTAGREMDQA
ITTVGRENIVGVYAANDGMAGAVVAALRRANVNPLPPVTGQDAELAGVQRVLAGDQHMTVYKAIRPEAEQAADLALALLR
GEPVDTIATGHVDNGNGQVPAVLLEPVAVTRDTVAATVVKDGFIAKADLCAGTYATACASAGIS

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=313, Percent_Identity=34.5047923322684, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1788473, Length=270, Percent_Identity=25.5555555555556, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40280; Mature: 40148

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNSLDDGVPRRGRMRGHAGRRRGRLFVAASVAVMVVLAAVGCTSDSSDDEVPQGGSES
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GTIALLLPETQTTRYESADRPYFEARMAKICPDCKVLYSNADQDSAAQQNQAEQAMTNGA
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCC
KVLVLDPVDGEAAAVIARNARDRGVRVVSYDRLIQKAPVDAYISFDNEKVGQLQGQALLD
EEEEEECCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
AIGDRAGAGKVIMINGSQDDPNAQQFKDGALSVLEGKVTIGFDTFTPDWSPDTAGREMDQ
HHCCCCCCCEEEEEECCCCCCCHHHHHHHCCEEEECEEEEEEECCCCCCCCCCCHHHHHH
AITTVGRENIVGVYAANDGMAGAVVAALRRANVNPLPPVTGQDAELAGVQRVLAGDQHMT
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYKAIRPEAEQAADLALALLRGEPVDTIATGHVDNGNGQVPAVLLEPVAVTRDTVAATVV
EHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCEECCCCCCCEEEECCHHHHHHHHHHHHH
KDGFIAKADLCAGTYATACASAGIS
HCCCEEEHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
ADNSLDDGVPRRGRMRGHAGRRRGRLFVAASVAVMVVLAAVGCTSDSSDDEVPQGGSES
CCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GTIALLLPETQTTRYESADRPYFEARMAKICPDCKVLYSNADQDSAAQQNQAEQAMTNGA
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCC
KVLVLDPVDGEAAAVIARNARDRGVRVVSYDRLIQKAPVDAYISFDNEKVGQLQGQALLD
EEEEEECCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
AIGDRAGAGKVIMINGSQDDPNAQQFKDGALSVLEGKVTIGFDTFTPDWSPDTAGREMDQ
HHCCCCCCCEEEEEECCCCCCCHHHHHHHCCEEEECEEEEEEECCCCCCCCCCCHHHHHH
AITTVGRENIVGVYAANDGMAGAVVAALRRANVNPLPPVTGQDAELAGVQRVLAGDQHMT
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
VYKAIRPEAEQAADLALALLRGEPVDTIATGHVDNGNGQVPAVLLEPVAVTRDTVAATVV
EHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCEECCCCCCCEEEECCHHHHHHHHHHHHH
KDGFIAKADLCAGTYATACASAGIS
HCCCEEEHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]