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The map label for this gene is pncA [H]

Identifier: 158316918

GI number: 158316918

Start: 6186964

End: 6187575

Strand: Direct

Name: pncA [H]

Synonym: Franean1_5162

Alternate gene names: 158316918

Gene position: 6186964-6187575 (Clockwise)

Preceding gene: 158316914

Following gene: 158316920

Centisome position: 68.88

GC content: 73.2

Gene sequence:

>612_bases
ATGGCAACGCGGTACGGGCCCGGCGTGGCGCTGGTCGTCCTCGACCTGCAGAACGACTTCGCCGCGCCCGGGGGCAGCCT
CTACGTCGACGGCGGCGAGGACATCGTCGCCGGGGTGAACCGGGAGATCGCGGCGGCGACGAGCGCGGGCTCCCCTGTCT
TCTACACCCAGGACTGGCATCCGCCGTCCACGCCGCATTTCGTCACCGAGGGCGGGATCTGGCCGCCGCACTGCGTGCGA
GACACGCCCGGGGCGCGCCTGTGCCCCGACCTGACGGTGGCCGGTGAGGTGATCCGCAAGGGCGTGGACGGCCACGACGG
GTACTCGGGCTTCTCCGTCCGTGACCCACGTTCGGGCGAGCAGTCGGCGACCGTGCTCGGCGAGCGGCTTGCCGCAGGCT
CCGTCCGCACGGTCGTGGTGGTGGGCCTGGCTGGTGACTACTGCGTGCTGGAGACGGCGCTCGACGCCCGTGCGCTGGGT
CTGGAGGTGACGCTCCCGCTGGAGCTGACCCGGTTCGTCGAGCTGGCGGAGGGTGATCGCGAACGCGCGATCGCCCGGAT
GGTCGGGGCGGGCGTGCGGGTGGAGGGCCTGGCCGCCAGGGGACGGGGCTGA

Upstream 100 bases:

>100_bases
GGAATCCGCGGGCGGGGCGCGGGGCAGTCTGCCGAGCGGCGCGGGCAGGCGCGTCCGGGGCCCGGGCGAGGCGCCGCCGG
ATCGGGATCGAGGTGACGCG

Downstream 100 bases:

>100_bases
CGCCGCCCCGCCGGGTCACCCGCCGCGGCGCTGCCGCGGGCTCCCGCGGCGGTGCCGCTGTCGGTGTGAAGCCGCTGTCA
GGCGGCGCAGTCCCGGCACA

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MATRYGPGVALVVLDLQNDFAAPGGSLYVDGGEDIVAGVNREIAAATSAGSPVFYTQDWHPPSTPHFVTEGGIWPPHCVR
DTPGARLCPDLTVAGEVIRKGVDGHDGYSGFSVRDPRSGEQSATVLGERLAAGSVRTVVVVGLAGDYCVLETALDARALG
LEVTLPLELTRFVELAEGDRERAIARMVGAGVRVEGLAARGRG

Sequences:

>Translated_203_residues
MATRYGPGVALVVLDLQNDFAAPGGSLYVDGGEDIVAGVNREIAAATSAGSPVFYTQDWHPPSTPHFVTEGGIWPPHCVR
DTPGARLCPDLTVAGEVIRKGVDGHDGYSGFSVRDPRSGEQSATVLGERLAAGSVRTVVVVGLAGDYCVLETALDARALG
LEVTLPLELTRFVELAEGDRERAIARMVGAGVRVEGLAARGRG
>Mature_202_residues
ATRYGPGVALVVLDLQNDFAAPGGSLYVDGGEDIVAGVNREIAAATSAGSPVFYTQDWHPPSTPHFVTEGGIWPPHCVRD
TPGARLCPDLTVAGEVIRKGVDGHDGYSGFSVRDPRSGEQSATVLGERLAAGSVRTVVVVGLAGDYCVLETALDARALGL
EVTLPLELTRFVELAEGDRERAIARMVGAGVRVEGLAARGRG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=210, Percent_Identity=35.2380952380952, Blast_Score=103, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321401, Length=192, Percent_Identity=29.1666666666667, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21180; Mature: 21049

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRYGPGVALVVLDLQNDFAAPGGSLYVDGGEDIVAGVNREIAAATSAGSPVFYTQDWH
CCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEECCCCCEEEECCCCCCEEEECCCC
PPSTPHFVTEGGIWPPHCVRDTPGARLCPDLTVAGEVIRKGVDGHDGYSGFSVRDPRSGE
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QSATVLGERLAAGSVRTVVVVGLAGDYCVLETALDARALGLEVTLPLELTRFVELAEGDR
HHHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHHCCCCEEEEEEECHHHHHHHHHHCCCH
ERAIARMVGAGVRVEGLAARGRG
HHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure 
ATRYGPGVALVVLDLQNDFAAPGGSLYVDGGEDIVAGVNREIAAATSAGSPVFYTQDWH
CCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCEEECCCCCEEEECCCCCCEEEECCCC
PPSTPHFVTEGGIWPPHCVRDTPGARLCPDLTVAGEVIRKGVDGHDGYSGFSVRDPRSGE
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QSATVLGERLAAGSVRTVVVVGLAGDYCVLETALDARALGLEVTLPLELTRFVELAEGDR
HHHHHHHHHHCCCCEEEEEEEECCCCEEEEEHHHCCCCEEEEEEECHHHHHHHHHHCCCH
ERAIARMVGAGVRVEGLAARGRG
HHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]