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The map label for this gene is pimA [H]

Identifier: 158316908

GI number: 158316908

Start: 6173266

End: 6174399

Strand: Reverse

Name: pimA [H]

Synonym: Franean1_5152

Alternate gene names: 158316908

Gene position: 6174399-6173266 (Counterclockwise)

Preceding gene: 158316909

Following gene: 158316907

Centisome position: 68.74

GC content: 74.34

Gene sequence:

>1134_bases
GTGAGAATCGGACTCGCCTGCCCCTACACCTGGGACGTGCCCGGGGGCGTCCAGGCGCACGTGCGTGACCTCGCCGAGAC
GTTGCTCGACGCCGGGCACGAGGTCTCGGTGATCACCCCGGTGGACGACGAGTCCACGCTGCCGGCGTACGCCGTGGACG
CCGGCCGCGCGGTGCCGGTGCCCTACAACGGCTCGGTGGCCCGGCTGCTCATGGGGCCGGTCTCGGCCGCCCGGGTGCGT
CGGTGGCTGCGCGAGCACGACTTCGACGTGCTGCACGCGCACGAGCCGACCGCGCCCAGCGTCAGCCTGCTGGCCTGCAT
GCTCGCGGACGGTCCGCTGGTGGCGACCTTCCACACCGCCAACCCCAGGTCAAGGATCCTCACCGCGGGGCACCGCGCGC
TGCAGCCGTCGCTGGAGAAGCTGCGGGCGCGGATCGCGGTCTCCGAGGCGGCGCGGCGCACGCTCGTGGAGCACCTCGGC
ACCGGCGCGATCCTCATCCCGAACGGAGTGGCCGTGCGGGCCTTCGAGGGCGCCCGCCCGCTGCCCGGCTACGGCACCGC
GCCGACGGTGGCGTTCCTCGGCCGGATCGACGAGCCGCGCAAGGGCCTGGACGTGCTGATGGCGGCGTTCCCGATGCTCG
TCGAGAAGGTGCCGGACGTCCGCCTGCTGGTCGCGGGCCCCGGCGACACCGAGGCGGTGCGCGCGCGCATCGACCCCGCG
CTGCTCGGCCGGGTCGATCTGGTCGGGCTCGTCCCGGAGGCCGACAAGGCCGCGGTGTTCGCCTCCGGCAGCGTGTACTG
TGCCCCGAACACAGGGCAGGAGAGCTTCGGGATCGTGCTGCTCGAGGCGATGGCGGCCGGGACGCCGGTCGTCGCGAGCG
ACATCGACGCGTTCCGGCGGGTGCTCGATAATGGGCGGGCCGGGCGGTTGTTCGGGGTGGGGGAACCGGCGGAGCTGGCG
GCGAACCTGGCCGAGCTGATCGAGGACCCGGCCGAGCGAGCCCGGCTGGCCGAGCGCGGCCGCGCCGTCGTCGCCCGGTA
CGACTGGCGGGTGATCGCCCAGAGCATCGTCGGCGTGTACGAGATGGTCTCCGGCGACCGCCCGGTGTCGGTCGCCGCCC
CCGAGGAGAGCTGA

Upstream 100 bases:

>100_bases
CGGCTCGCGGACGAGTTCGCCTCCGGCATCGCCGCCCATCCGTCCGACTGGCACATGCTCCAGCGGATCTGGCCCGACCG
GTCATGAGGAGCAGCCGGCG

Downstream 100 bases:

>100_bases
GCCGGCCGGGCGGTATCCCGCGGCGGCCGGACTGCGGCGCCGGCCGGTATCCCGTGCCTGGCGATATCCCGCGCCGGCCG
ATATCACCGACCGGCCGTGA

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: Alpha-mannosyltransferase; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MRIGLACPYTWDVPGGVQAHVRDLAETLLDAGHEVSVITPVDDESTLPAYAVDAGRAVPVPYNGSVARLLMGPVSAARVR
RWLREHDFDVLHAHEPTAPSVSLLACMLADGPLVATFHTANPRSRILTAGHRALQPSLEKLRARIAVSEAARRTLVEHLG
TGAILIPNGVAVRAFEGARPLPGYGTAPTVAFLGRIDEPRKGLDVLMAAFPMLVEKVPDVRLLVAGPGDTEAVRARIDPA
LLGRVDLVGLVPEADKAAVFASGSVYCAPNTGQESFGIVLLEAMAAGTPVVASDIDAFRRVLDNGRAGRLFGVGEPAELA
ANLAELIEDPAERARLAERGRAVVARYDWRVIAQSIVGVYEMVSGDRPVSVAAPEES

Sequences:

>Translated_377_residues
MRIGLACPYTWDVPGGVQAHVRDLAETLLDAGHEVSVITPVDDESTLPAYAVDAGRAVPVPYNGSVARLLMGPVSAARVR
RWLREHDFDVLHAHEPTAPSVSLLACMLADGPLVATFHTANPRSRILTAGHRALQPSLEKLRARIAVSEAARRTLVEHLG
TGAILIPNGVAVRAFEGARPLPGYGTAPTVAFLGRIDEPRKGLDVLMAAFPMLVEKVPDVRLLVAGPGDTEAVRARIDPA
LLGRVDLVGLVPEADKAAVFASGSVYCAPNTGQESFGIVLLEAMAAGTPVVASDIDAFRRVLDNGRAGRLFGVGEPAELA
ANLAELIEDPAERARLAERGRAVVARYDWRVIAQSIVGVYEMVSGDRPVSVAAPEES
>Mature_377_residues
MRIGLACPYTWDVPGGVQAHVRDLAETLLDAGHEVSVITPVDDESTLPAYAVDAGRAVPVPYNGSVARLLMGPVSAARVR
RWLREHDFDVLHAHEPTAPSVSLLACMLADGPLVATFHTANPRSRILTAGHRALQPSLEKLRARIAVSEAARRTLVEHLG
TGAILIPNGVAVRAFEGARPLPGYGTAPTVAFLGRIDEPRKGLDVLMAAFPMLVEKVPDVRLLVAGPGDTEAVRARIDPA
LLGRVDLVGLVPEADKAAVFASGSVYCAPNTGQESFGIVLLEAMAAGTPVVASDIDAFRRVLDNGRAGRLFGVGEPAELA
ANLAELIEDPAERARLAERGRAVVARYDWRVIAQSIVGVYEMVSGDRPVSVAAPEES

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Contrary to pimB, the mannosyltransferase pim

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI9755344, Length=383, Percent_Identity=22.4543080939948, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 39930; Mature: 39930

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGLACPYTWDVPGGVQAHVRDLAETLLDAGHEVSVITPVDDESTLPAYAVDAGRAVPV
CEEEEECCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCEECC
PYNGSVARLLMGPVSAARVRRWLREHDFDVLHAHEPTAPSVSLLACMLADGPLVATFHTA
CCCCCHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECC
NPRSRILTAGHRALQPSLEKLRARIAVSEAARRTLVEHLGTGAILIPNGVAVRAFEGARP
CCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCC
LPGYGTAPTVAFLGRIDEPRKGLDVLMAAFPMLVEKVPDVRLLVAGPGDTEAVRARIDPA
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCHH
LLGRVDLVGLVPEADKAAVFASGSVYCAPNTGQESFGIVLLEAMAAGTPVVASDIDAFRR
HHCCCCEEEECCCCCCCEEEECCEEEECCCCCCCHHHHHHEEHHHCCCCHHHHHHHHHHH
VLDNGRAGRLFGVGEPAELAANLAELIEDPAERARLAERGRAVVARYDWRVIAQSIVGVY
HHCCCCCCEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
EMVSGDRPVSVAAPEES
HHHCCCCCEEECCCCCC
>Mature Secondary Structure
MRIGLACPYTWDVPGGVQAHVRDLAETLLDAGHEVSVITPVDDESTLPAYAVDAGRAVPV
CEEEEECCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCEECC
PYNGSVARLLMGPVSAARVRRWLREHDFDVLHAHEPTAPSVSLLACMLADGPLVATFHTA
CCCCCHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECC
NPRSRILTAGHRALQPSLEKLRARIAVSEAARRTLVEHLGTGAILIPNGVAVRAFEGARP
CCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCC
LPGYGTAPTVAFLGRIDEPRKGLDVLMAAFPMLVEKVPDVRLLVAGPGDTEAVRARIDPA
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCHH
LLGRVDLVGLVPEADKAAVFASGSVYCAPNTGQESFGIVLLEAMAAGTPVVASDIDAFRR
HHCCCCEEEECCCCCCCEEEECCEEEECCCCCCCHHHHHHEEHHHCCCCHHHHHHHHHHH
VLDNGRAGRLFGVGEPAELAANLAELIEDPAERARLAERGRAVVARYDWRVIAQSIVGVY
HHCCCCCCEEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
EMVSGDRPVSVAAPEES
HHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12068013 [H]