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The map label for this gene is pdxT

Identifier: 158316904

GI number: 158316904

Start: 6169450

End: 6170061

Strand: Reverse

Name: pdxT

Synonym: Franean1_5148

Alternate gene names: 158316904

Gene position: 6170061-6169450 (Counterclockwise)

Preceding gene: 158316905

Following gene: 158316903

Centisome position: 68.69

GC content: 72.71

Gene sequence:

>612_bases
GTGAGCGCCCCACGGATCGGCGTCCTGGCGCTGCAGGGCGATGTGCGCGAGCACGTCCGCGCCCTGCGCGAGGCCGGAGC
CGACGCCGGTGAGGTGCGCCGGGCCGGTGAGCTCGCCGAGCTGGACGGCCTGGTCCTGCCCGGTGGCGAGTCCACTACGA
TCGGGCGGTTGCTGCGCGTGTTCGACCTGCTCGAACCGCTGCGGGCCGCCGTGGCCGGTGGTCTGCCGGTGTTCGGCTCG
TGCGCGGGCATGATCCTGCTCGCGCAGGATGTGATCGACGGCCGCCCGGGCCAGCCGCTCATCGGTGGGCTCGACGTGGT
CGTCCGGCGCAACGCCTTCGGCCGCCAGGTGGAGTCGTTCGAGACCGACCTGGTGGTGGACGGCGTCGACGGGCCGGCGG
TGCACGCCGTCTTCATCCGGGCACCGTGGGTAGAAAAGGCGGGGGACGGCGTCGAGGTACTGGCCCGGGTCGCCGACCGG
CCGGTCGCGGTCCGGCAGGGGCCGCTGCTGGCCACGTCGTTCCATCCTGAACTGACGGGGGACATCCGGGTGCACCGGCT
GTTCGTGGAGATCGTCCGATCCGAGCTCGGGCTCAAGAGGGGCAGGTCATGA

Upstream 100 bases:

>100_bases
CCGGCGTGCTGGCGAAGGTGTCGCGCGGCCTCGGTGAGGCCATGGTCGGCATCAACGTCGGCGAGCTCCCGCCCGAGGCG
CGCTTCGCCGCCCGCGGCTG

Downstream 100 bases:

>100_bases
GCGGTCACTCCAAGTGGGCGACCACCAAGCACAAGAAGGCGGTCGTCGACGCCCGCCGGGGCAAGCTGTTCGCCAAGCTG
GTCAAGACCGTCGAAGTCGC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSAPRIGVLALQGDVREHVRALREAGADAGEVRRAGELAELDGLVLPGGESTTIGRLLRVFDLLEPLRAAVAGGLPVFGS
CAGMILLAQDVIDGRPGQPLIGGLDVVVRRNAFGRQVESFETDLVVDGVDGPAVHAVFIRAPWVEKAGDGVEVLARVADR
PVAVRQGPLLATSFHPELTGDIRVHRLFVEIVRSELGLKRGRS

Sequences:

>Translated_203_residues
MSAPRIGVLALQGDVREHVRALREAGADAGEVRRAGELAELDGLVLPGGESTTIGRLLRVFDLLEPLRAAVAGGLPVFGS
CAGMILLAQDVIDGRPGQPLIGGLDVVVRRNAFGRQVESFETDLVVDGVDGPAVHAVFIRAPWVEKAGDGVEVLARVADR
PVAVRQGPLLATSFHPELTGDIRVHRLFVEIVRSELGLKRGRS
>Mature_202_residues
SAPRIGVLALQGDVREHVRALREAGADAGEVRRAGELAELDGLVLPGGESTTIGRLLRVFDLLEPLRAAVAGGLPVFGSC
AGMILLAQDVIDGRPGQPLIGGLDVVVRRNAFGRQVESFETDLVVDGVDGPAVHAVFIRAPWVEKAGDGVEVLARVADRP
VAVRQGPLLATSFHPELTGDIRVHRLFVEIVRSELGLKRGRS

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323995, Length=211, Percent_Identity=36.0189573459716, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=211, Percent_Identity=35.5450236966825, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323742, Length=206, Percent_Identity=36.4077669902913, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_FRASN (A8KZF0)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001509412.1
- ProteinModelPortal:   A8KZF0
- SMR:   A8KZF0
- GeneID:   5673482
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_5148
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   FSP1855:FRANEAN1_5148-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21541; Mature: 21410

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 81-81 ACT_SITE 175-175 ACT_SITE 177-177 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRIGVLALQGDVREHVRALREAGADAGEVRRAGELAELDGLVLPGGESTTIGRLLRV
CCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHCCHHHHCCEEECCCCCHHHHHHHHH
FDLLEPLRAAVAGGLPVFGSCAGMILLAQDVIDGRPGQPLIGGLDVVVRRNAFGRQVESF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHC
ETDLVVDGVDGPAVHAVFIRAPWVEKAGDGVEVLARVADRPVAVRQGPLLATSFHPELTG
CCCEEEECCCCCCEEEEEEECCCHHCCCCHHHHHHHHHCCCEEECCCCEEEECCCCCCCC
DIRVHRLFVEIVRSELGLKRGRS
CHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAPRIGVLALQGDVREHVRALREAGADAGEVRRAGELAELDGLVLPGGESTTIGRLLRV
CCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHCCHHHHCCEEECCCCCHHHHHHHHH
FDLLEPLRAAVAGGLPVFGSCAGMILLAQDVIDGRPGQPLIGGLDVVVRRNAFGRQVESF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHC
ETDLVVDGVDGPAVHAVFIRAPWVEKAGDGVEVLARVADRPVAVRQGPLLATSFHPELTG
CCCEEEECCCCCCEEEEEEECCCHHCCCCHHHHHHHHHCCCEEECCCCEEEECCCCCCCC
DIRVHRLFVEIVRSELGLKRGRS
CHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA