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The map label for this gene is mraW

Identifier: 158316863

GI number: 158316863

Start: 6114381

End: 6115364

Strand: Reverse

Name: mraW

Synonym: Franean1_5106

Alternate gene names: 158316863

Gene position: 6115364-6114381 (Counterclockwise)

Preceding gene: 158316864

Following gene: 158316862

Centisome position: 68.08

GC content: 73.88

Gene sequence:

>984_bases
GTGGACGTCACGCACACGCCGGTGCTGACCGAGCGCGTGATCCAGCTGCTCGACCCCGCGCTGCGCCGTCCCGGCGCGGT
CGTCGTCGACGCCACGGTCGGGCTGGGCGGTCACTCGCACGCGCTGCTCGAGGCCTTCCCGCAGGTCCGCCTCATCGGTC
TCGACCGGGACACCACCGCCCTCGAGCACAGCCGGCGCCGCCTCACCGGCCTGGGTGACCGCGTCGACCTCGAGCACGCC
GTCTACGACCAGCTGCCGGACGTCCTCGATCGGCTCGGCGTGCCGTCGGTGGACGGTGTCCTGTTCGATCTCGGCGTCTC
GTCCATGCAGCTCGACCTGGACGAGCGCGGCTTCGCCTACTCCCGGGACGCCCCGCTGGACATGCGGATGGACCAGGAGC
GCGGCCCCACCGCGGCCGACGTCCTCAACAACTACGACGTCGACGCGCTGACCCGGGTGCTGCGGGAGTACGGCGAGGAG
CGGTTCGCGCGCCGCATCGCCCGGGCGGTGGTGACCCAGCGCCAGGCCGCGCCGTTCACCACCTCCGCGCGGCTGGTGGA
CCTGGTCCGCGACTCCATCCCCGCGGCGGCGCGCCGCACCGGCGGCAACCCGGCCAAGCGCACCTTCCAGGCGCTGCGCA
TCGAGGTGAACTCCGAGCTCGAGGTGCTCGAGCGTGCGGTGCCCGCCGCCTTCGACGCGCTGGCCGTGGCGGGCCGCCTG
GTCGTCCTCTCCTATCACTCCCTGGAGGACCGGCTGGTGAAGCGGGTGCTGATGCCGCGCTCGGTGCCCTCCGTCCCGCC
GGACATGCCGGTCATCCCGGATGACGCCCGGCCAACGCTGCGGTGGCTGACCCGCGGCGCGGAGCCGGCTTCCGACACGG
AGATCGAACAGAACGCGCGCGCCGGGTCTGTCCGGCTGCGCGCGGCGGAACGCACGGCGGCCGAACCGGGCCGCGCGCAC
AACCCGACCGGAGGTGTCCGATGA

Upstream 100 bases:

>100_bases
CTTCCCGATGGCCGCCGGGGACGGCGTACCCGCCAGCGCACCGGCGTGCCCGCCCGCATATCCGAAACACCAGATCCGAC
GAGCGGCCGAGGGGTTGGCA

Downstream 100 bases:

>100_bases
CCGCTCCCGCGCACCCGCTGCCCCGCAGCGGTCGCGCCCGTCCGGGTCGTCGCGCCTCCGGGTCCGAGCACCACGCGACA
GACCACGACGCGGCCGCCTA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MDVTHTPVLTERVIQLLDPALRRPGAVVVDATVGLGGHSHALLEAFPQVRLIGLDRDTTALEHSRRRLTGLGDRVDLEHA
VYDQLPDVLDRLGVPSVDGVLFDLGVSSMQLDLDERGFAYSRDAPLDMRMDQERGPTAADVLNNYDVDALTRVLREYGEE
RFARRIARAVVTQRQAAPFTTSARLVDLVRDSIPAAARRTGGNPAKRTFQALRIEVNSELEVLERAVPAAFDALAVAGRL
VVLSYHSLEDRLVKRVLMPRSVPSVPPDMPVIPDDARPTLRWLTRGAEPASDTEIEQNARAGSVRLRAAERTAAEPGRAH
NPTGGVR

Sequences:

>Translated_327_residues
MDVTHTPVLTERVIQLLDPALRRPGAVVVDATVGLGGHSHALLEAFPQVRLIGLDRDTTALEHSRRRLTGLGDRVDLEHA
VYDQLPDVLDRLGVPSVDGVLFDLGVSSMQLDLDERGFAYSRDAPLDMRMDQERGPTAADVLNNYDVDALTRVLREYGEE
RFARRIARAVVTQRQAAPFTTSARLVDLVRDSIPAAARRTGGNPAKRTFQALRIEVNSELEVLERAVPAAFDALAVAGRL
VVLSYHSLEDRLVKRVLMPRSVPSVPPDMPVIPDDARPTLRWLTRGAEPASDTEIEQNARAGSVRLRAAERTAAEPGRAH
NPTGGVR
>Mature_327_residues
MDVTHTPVLTERVIQLLDPALRRPGAVVVDATVGLGGHSHALLEAFPQVRLIGLDRDTTALEHSRRRLTGLGDRVDLEHA
VYDQLPDVLDRLGVPSVDGVLFDLGVSSMQLDLDERGFAYSRDAPLDMRMDQERGPTAADVLNNYDVDALTRVLREYGEE
RFARRIARAVVTQRQAAPFTTSARLVDLVRDSIPAAARRTGGNPAKRTFQALRIEVNSELEVLERAVPAAFDALAVAGRL
VVLSYHSLEDRLVKRVLMPRSVPSVPPDMPVIPDDARPTLRWLTRGAEPASDTEIEQNARAGSVRLRAAERTAAEPGRAH
NPTGGVR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=270, Percent_Identity=41.8518518518518, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI165377202, Length=194, Percent_Identity=40.7216494845361, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1786270, Length=320, Percent_Identity=40.625, Blast_Score=201, Evalue=7e-53,
Organism=Drosophila melanogaster, GI62472493, Length=339, Percent_Identity=35.9882005899705, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28571637, Length=339, Percent_Identity=35.9882005899705, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_FRASN (A8KZA9)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001509371.1
- ProteinModelPortal:   A8KZA9
- SMR:   A8KZA9
- GeneID:   5673441
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_5106
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   FSP1855:FRANEAN1_5106-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35867; Mature: 35867

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 89-89 BINDING 103-103 BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVTHTPVLTERVIQLLDPALRRPGAVVVDATVGLGGHSHALLEAFPQVRLIGLDRDTTA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCEEEEECCCCHHH
LEHSRRRLTGLGDRVDLEHAVYDQLPDVLDRLGVPSVDGVLFDLGVSSMQLDLDERGFAY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCCCCCEE
SRDAPLDMRMDQERGPTAADVLNNYDVDALTRVLREYGEERFARRIARAVVTQRQAAPFT
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSARLVDLVRDSIPAAARRTGGNPAKRTFQALRIEVNSELEVLERAVPAAFDALAVAGRL
HHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VVLSYHSLEDRLVKRVLMPRSVPSVPPDMPVIPDDARPTLRWLTRGAEPASDTEIEQNAR
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCC
AGSVRLRAAERTAAEPGRAHNPTGGVR
CCCEEEEEHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDVTHTPVLTERVIQLLDPALRRPGAVVVDATVGLGGHSHALLEAFPQVRLIGLDRDTTA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCEEEEECCCCHHH
LEHSRRRLTGLGDRVDLEHAVYDQLPDVLDRLGVPSVDGVLFDLGVSSMQLDLDERGFAY
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEECCCCCCCEE
SRDAPLDMRMDQERGPTAADVLNNYDVDALTRVLREYGEERFARRIARAVVTQRQAAPFT
CCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSARLVDLVRDSIPAAARRTGGNPAKRTFQALRIEVNSELEVLERAVPAAFDALAVAGRL
HHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VVLSYHSLEDRLVKRVLMPRSVPSVPPDMPVIPDDARPTLRWLTRGAEPASDTEIEQNAR
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHCCC
AGSVRLRAAERTAAEPGRAHNPTGGVR
CCCEEEEEHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA