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The map label for this gene is mshC

Identifier: 158316655

GI number: 158316655

Start: 5870770

End: 5872104

Strand: Reverse

Name: mshC

Synonym: Franean1_4892

Alternate gene names: 158316655

Gene position: 5872104-5870770 (Counterclockwise)

Preceding gene: 158316656

Following gene: 158316654

Centisome position: 65.38

GC content: 73.93

Gene sequence:

>1335_bases
GTGCCCTGCTCGTCGCCGGCCTGCCCGGCTTGTCCCGCGGCCGGCCCAGCCCCGGTTACCCTGACACGCATGCAGGCGTG
GCCCTTTCCCCAGCTCCCCAAGCTTCCCGGACAGGGTCGCGACCTCCGCGTTCTCGACACGGCGCACGGCGGAGTGCGGA
CTCTGGACCTCGGGCCGACGGTCCGGCTCTACGCCTGCGGGATCACCCCGTATGACGCCACCCACCTCGGGCACGCCTTC
ACGTACCTCACCTACGACCTCGTCCAGCGCGTGCTGCGGGACGCCGGCCACGAGGTGCGGTACGTGCAGAACGTCACCGA
CGTCGACGATCCGCTGCTCGAGCGGGCCACCCGCGACGGCATCGACTGGCGCGACCTCGCCCGCCGCGAGATCGACCTGT
TCCGGGCCGACATGACGGCGCTGCGCATCCTGCCCCCCGACCACTACGTCGGCGTCGTCGAGGCCGTCGGGCTCATCGTC
GACATGGTCTCCCAGCTCGTCGAGCGCGGCGCCGCGTACTCCGTCGACGGCGATCTCTACTTCTCCGTCGCCGCGGCGCC
GGACTTCGGCCAGGTGGCCCACCTCGACCCCGCGCAGATGCTGGTGTCCTGCGCCGAGCACGGCGGTGATCCCGGCCGGC
CGGGCAAGAAGGACCCGCTCGACCCGCTGCTGTGGCGCGCGGAGCGGCCGGGGGAGCCGTCCTGGCCGTCGCCGTTCGGC
CCGGGGCGGCCGGGCTGGCACGTGGAGTGCTCCGCGATCGCCCGGCACTACCTCGGCGCCACGATCGACATCCAGGGCGG
CGGGAGCGATCTCGCCTTCCCACACCACGAGTGCAGCGCCGCGCACGCCGAGGTCGCGAACGGCGCCCGGCCGTTCGCCC
GCGCCTACGTCCACACCGCGCTGGTCAGCCTGGACGGCCACAAGATGTCGAAGTCGCGGGGGAACCTCGAGTTCGTCTCG
AGGCTGCTCGCGCGCGGAGCGGACCCGGCCGCGATCAGGCTCGCCCTGTTGCAGCATCATCACACCGTGGAGTGGGAGTG
GACCGCGGCCGCCATGCCGGCGGCAGCCGAGCGGCTCGACCGGTGGCGCGCCGCCGTCGCGCTGCCGTCCGGCCCAGACT
TCCGGCCGGTGCTGGCCGAGGTCCGGGACCGGCTCGCCGACGACCTCGACGCGCCTGGAGCGCTCGCCGCGGTCGACGCG
TGGGCGGCGGCCGCACTGGCCGCTGGCAGCGGCGGCAGCGGCGAGGCGGACGACCAGGCGCCGGCCACCGTCCGCGACAC
CGTCGACTCACTGCTCGGTGTCGACCTTGGGCCTGTCCTACCGAGAGGAACCTAG

Upstream 100 bases:

>100_bases
TTGCCCTCCGGGGACTGGCCGGCCATTCCCTGGCCGCCGTTCTGAGCCGGCGGACGCGGCCCGACGGGGCCGCCGGCCGC
GACACGCCCGGGCGGGGGTT

Downstream 100 bases:

>100_bases
CCGATGGGGGCGCACCACCGCCGGGAGTCCCCCGGCGCCGCCATGGACCGGCCGACCTTTGATGAGGCACCGCAGGGATA
CGACCCCCGGCAGGTCGACG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: L-Cys:GlcN-Ins ligase; Mycothiol ligase; MSH ligase

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPCSSPACPACPAAGPAPVTLTRMQAWPFPQLPKLPGQGRDLRVLDTAHGGVRTLDLGPTVRLYACGITPYDATHLGHAF
TYLTYDLVQRVLRDAGHEVRYVQNVTDVDDPLLERATRDGIDWRDLARREIDLFRADMTALRILPPDHYVGVVEAVGLIV
DMVSQLVERGAAYSVDGDLYFSVAAAPDFGQVAHLDPAQMLVSCAEHGGDPGRPGKKDPLDPLLWRAERPGEPSWPSPFG
PGRPGWHVECSAIARHYLGATIDIQGGGSDLAFPHHECSAAHAEVANGARPFARAYVHTALVSLDGHKMSKSRGNLEFVS
RLLARGADPAAIRLALLQHHHTVEWEWTAAAMPAAAERLDRWRAAVALPSGPDFRPVLAEVRDRLADDLDAPGALAAVDA
WAAAALAAGSGGSGEADDQAPATVRDTVDSLLGVDLGPVLPRGT

Sequences:

>Translated_444_residues
MPCSSPACPACPAAGPAPVTLTRMQAWPFPQLPKLPGQGRDLRVLDTAHGGVRTLDLGPTVRLYACGITPYDATHLGHAF
TYLTYDLVQRVLRDAGHEVRYVQNVTDVDDPLLERATRDGIDWRDLARREIDLFRADMTALRILPPDHYVGVVEAVGLIV
DMVSQLVERGAAYSVDGDLYFSVAAAPDFGQVAHLDPAQMLVSCAEHGGDPGRPGKKDPLDPLLWRAERPGEPSWPSPFG
PGRPGWHVECSAIARHYLGATIDIQGGGSDLAFPHHECSAAHAEVANGARPFARAYVHTALVSLDGHKMSKSRGNLEFVS
RLLARGADPAAIRLALLQHHHTVEWEWTAAAMPAAAERLDRWRAAVALPSGPDFRPVLAEVRDRLADDLDAPGALAAVDA
WAAAALAAGSGGSGEADDQAPATVRDTVDSLLGVDLGPVLPRGT
>Mature_443_residues
PCSSPACPACPAAGPAPVTLTRMQAWPFPQLPKLPGQGRDLRVLDTAHGGVRTLDLGPTVRLYACGITPYDATHLGHAFT
YLTYDLVQRVLRDAGHEVRYVQNVTDVDDPLLERATRDGIDWRDLARREIDLFRADMTALRILPPDHYVGVVEAVGLIVD
MVSQLVERGAAYSVDGDLYFSVAAAPDFGQVAHLDPAQMLVSCAEHGGDPGRPGKKDPLDPLLWRAERPGEPSWPSPFGP
GRPGWHVECSAIARHYLGATIDIQGGGSDLAFPHHECSAAHAEVANGARPFARAYVHTALVSLDGHKMSKSRGNLEFVSR
LLARGADPAAIRLALLQHHHTVEWEWTAAAMPAAAERLDRWRAAVALPSGPDFRPVLAEVRDRLADDLDAPGALAAVDAW
AAAALAAGSGGSGEADDQAPATVRDTVDSLLGVDLGPVLPRGT

Specific function: Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily

Homologues:

Organism=Homo sapiens, GI13375695, Length=357, Percent_Identity=34.1736694677871, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=37.7682403433476, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=37.7682403433476, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=37.7682403433476, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=37.7682403433476, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1786737, Length=346, Percent_Identity=35.8381502890173, Blast_Score=224, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI17509797, Length=262, Percent_Identity=31.2977099236641, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17509795, Length=262, Percent_Identity=31.2977099236641, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324082, Length=256, Percent_Identity=34.765625, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922218, Length=363, Percent_Identity=34.9862258953168, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI19922364, Length=344, Percent_Identity=30.5232558139535, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSHC_FRASN (A8LH67)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001509163.1
- ProteinModelPortal:   A8LH67
- SMR:   A8LH67
- GeneID:   5673232
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_4892
- HOGENOM:   HBG327651
- OMA:   ALFREDM
- ProtClustDB:   PRK12418
- BioCyc:   FSP1855:FRANEAN1_4892-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01697
- InterPro:   IPR015803
- InterPro:   IPR017812
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- TIGRFAMs:   TIGR03447

Pfam domain/function: PF01406 tRNA-synt_1e

EC number: =6.3.1.13

Molecular weight: Translated: 47429; Mature: 47298

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 246-246 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCSSPACPACPAAGPAPVTLTRMQAWPFPQLPKLPGQGRDLRVLDTAHGGVRTLDLGPT
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCC
VRLYACGITPYDATHLGHAFTYLTYDLVQRVLRDAGHEVRYVQNVTDVDDPLLERATRDG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCHHHHHHHHHCC
IDWRDLARREIDLFRADMTALRILPPDHYVGVVEAVGLIVDMVSQLVERGAAYSVDGDLY
CCHHHHHHHHHHHHHHCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEE
FSVAAAPDFGQVAHLDPAQMLVSCAEHGGDPGRPGKKDPLDPLLWRAERPGEPSWPSPFG
EEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
PGRPGWHVECSAIARHYLGATIDIQGGGSDLAFPHHECSAAHAEVANGARPFARAYVHTA
CCCCCCEEEHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LVSLDGHKMSKSRGNLEFVSRLLARGADPAAIRLALLQHHHTVEWEWTAAAMPAAAERLD
HHHCCCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
RWRAAVALPSGPDFRPVLAEVRDRLADDLDAPGALAAVDAWAAAALAAGSGGSGEADDQA
HHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PATVRDTVDSLLGVDLGPVLPRGT
CCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PCSSPACPACPAAGPAPVTLTRMQAWPFPQLPKLPGQGRDLRVLDTAHGGVRTLDLGPT
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCC
VRLYACGITPYDATHLGHAFTYLTYDLVQRVLRDAGHEVRYVQNVTDVDDPLLERATRDG
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHCCCCCCHHHHHHHHHCC
IDWRDLARREIDLFRADMTALRILPPDHYVGVVEAVGLIVDMVSQLVERGAAYSVDGDLY
CCHHHHHHHHHHHHHHCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEE
FSVAAAPDFGQVAHLDPAQMLVSCAEHGGDPGRPGKKDPLDPLLWRAERPGEPSWPSPFG
EEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
PGRPGWHVECSAIARHYLGATIDIQGGGSDLAFPHHECSAAHAEVANGARPFARAYVHTA
CCCCCCEEEHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LVSLDGHKMSKSRGNLEFVSRLLARGADPAAIRLALLQHHHTVEWEWTAAAMPAAAERLD
HHHCCCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
RWRAAVALPSGPDFRPVLAEVRDRLADDLDAPGALAAVDAWAAAALAAGSGGSGEADDQA
HHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
PATVRDTVDSLLGVDLGPVLPRGT
CCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA