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The map label for this gene is kstD [H]

Identifier: 158316518

GI number: 158316518

Start: 5675616

End: 5677280

Strand: Reverse

Name: kstD [H]

Synonym: Franean1_4753

Alternate gene names: 158316518

Gene position: 5677280-5675616 (Counterclockwise)

Preceding gene: 158316520

Following gene: 158316517

Centisome position: 63.21

GC content: 73.81

Gene sequence:

>1665_bases
ATGGATGAGCATGAGACGTACGACGTGGTGGTTCTGGGGTCAGGCGCGGCGGGGCTCACCGCGGCGCTCGCCGCGGCCGT
GGAGGGTGCCCGCGTCGCCGTGTTCGAGAAGGCGGAGCTGCTCGGCGGCACGACCGCGCTGTCCGGCGGGACGATCTGGG
TCCCCGCCAACCAGCCGGCCCGGGCGGCCGGCATCAAGGATTCCCGCGCGGACGGCCTGGCGTACCTGGCGAGTCTGTCG
AACGGGATGATTCTCCCGGAGCTGGCCGAGGCGCTCGTCGACGGCGGTCCGGAGCTGGTCGACTTCGTCGAGCAGCAGAC
GGATCTCCGTTTCCACCTGGTCGAGGGCTATCCCGACTATCACCCGGAACGTCCCGGCGGCCTGCCCCGCGGGGGACGGT
CGATCGAGGTCGGCCTCTTCTCCTTCCTCGGGTTCGAGGAATGGGAGGACCGCATCGCGGGCGACACCCGCCGGCTGCTC
ATCCGCGAGATGCCCCTGGGCGGGGGCACCGGCGCGCTGGAGCTGGACGTGCTCGCCGAGCGGGAGAAGAACCGCGCGGA
GGGCCTCGGCCGGGCGCTGGTCGGCGGTCTGCTCCAGGCCTGCCTGCGGCGTGGGGTGCACGTCGCCACCCGGTCGCGCG
GCCTGAGGTTGGTCACCGGTGACGGCGCCGTCACCGGGATCGAGATCGCGACCCCCGACGGCACCCGGTCGGTGCGCACG
GGCGCGGTCGTGCTCGCCACCGGCGGCTTCGAGTACGACGCCGATCTCGTCCGCGACTTCCTGCGCGGGCCGCTGACCCG
GGCCATCGGCGCGCCGTCCAACACGGGGGACGGGCTGCGCATGGCGATGCGGGTCGGCGCCCAGCTCGGCAACATGCGGG
AGGCCTGGTGGGCCCCGGTCGTCAGCCTTCCCGGGCATCGCCGTGACGGCGCCCGCAACGGTCTGCTGACCGCCCGGGAG
CGCGCACTGCCCGGTTCGATCATGGTCAACGGCCACGGCCGCCGGTTCGCCAACGAGGCGGCGAACTACAACGCCTTCGG
CGGCGCCTACCACCAGCTCGACGCGTCGAAGTTCGAGTACCCGAACCTGCCGAGCTACCTGATCTTCGACCAGTCGACCG
TCGACCGGTTCGGTGTCTTCGGCGGGGCGCCCGGCGGCGTGGTGCCGGACTGGCTGGAGCGCGCGGACACCCTGGACGCG
CTGGCGGGTCGGCTCGGCCTGCCCGCGGCCGAGGTCGCGGCGACCGTGCGGCGGTTCAACGAGCATGCCCGTGCCGGCAC
CGACCCCGACTTCGGCCGCGGCGACAACGCCTACGACCGGTTCCCCGGCGGCCGGGTGCTCGATCCCGAATCGGTCCTCT
CCACGCTGGGCCCGGTCGAGACCGCGCCGTTCTACGGCGTCGAGGTCGGGTCGTCGGCGCTCGGCACCAAAGGCGGGCCC
CGGACCGATCGGGAGGGCCGGGTCCTGGACGTCGACGGCGACGTGATCCGCGGGCTCTACGCCGCGGGGAACGTGATGGC
GAGCCCGTCCGGCATGGTGTACGGCGGTGCCGGGGCGACACTGGCCGTCGCCGGGGTGTGGGGTCTCTACGCCGGGCGCG
CGGCGGCGCGCGACCCGGCGTCCGGCGCCCGGTCGCCGGTCCTGAGTGAACGATCCGGAAGCTGA

Upstream 100 bases:

>100_bases
CGGCCCAGGCGGCCACCGCGGCCCGCGGGCGCCGCGCCGACGCGGTCCGGTGCCGGCCCGCCGGGCGGTTCCGAAACTCT
CCCAGTGACGGGGGCATGCG

Downstream 100 bases:

>100_bases
CCCGAAGCCAGTAGATCATCGGGAGTTGGGGCGATGAGCGAGACAGTGACCGAGACTTTAGAGAGCCCGGCCGAGGCGAT
CACCGCGCGGGACGCCGAGG

Product: fumarate reductase/succinate dehydrogenase flavoprotein domain-containing protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MDEHETYDVVVLGSGAAGLTAALAAAVEGARVAVFEKAELLGGTTALSGGTIWVPANQPARAAGIKDSRADGLAYLASLS
NGMILPELAEALVDGGPELVDFVEQQTDLRFHLVEGYPDYHPERPGGLPRGGRSIEVGLFSFLGFEEWEDRIAGDTRRLL
IREMPLGGGTGALELDVLAEREKNRAEGLGRALVGGLLQACLRRGVHVATRSRGLRLVTGDGAVTGIEIATPDGTRSVRT
GAVVLATGGFEYDADLVRDFLRGPLTRAIGAPSNTGDGLRMAMRVGAQLGNMREAWWAPVVSLPGHRRDGARNGLLTARE
RALPGSIMVNGHGRRFANEAANYNAFGGAYHQLDASKFEYPNLPSYLIFDQSTVDRFGVFGGAPGGVVPDWLERADTLDA
LAGRLGLPAAEVAATVRRFNEHARAGTDPDFGRGDNAYDRFPGGRVLDPESVLSTLGPVETAPFYGVEVGSSALGTKGGP
RTDREGRVLDVDGDVIRGLYAAGNVMASPSGMVYGGAGATLAVAGVWGLYAGRAAARDPASGARSPVLSERSGS

Sequences:

>Translated_554_residues
MDEHETYDVVVLGSGAAGLTAALAAAVEGARVAVFEKAELLGGTTALSGGTIWVPANQPARAAGIKDSRADGLAYLASLS
NGMILPELAEALVDGGPELVDFVEQQTDLRFHLVEGYPDYHPERPGGLPRGGRSIEVGLFSFLGFEEWEDRIAGDTRRLL
IREMPLGGGTGALELDVLAEREKNRAEGLGRALVGGLLQACLRRGVHVATRSRGLRLVTGDGAVTGIEIATPDGTRSVRT
GAVVLATGGFEYDADLVRDFLRGPLTRAIGAPSNTGDGLRMAMRVGAQLGNMREAWWAPVVSLPGHRRDGARNGLLTARE
RALPGSIMVNGHGRRFANEAANYNAFGGAYHQLDASKFEYPNLPSYLIFDQSTVDRFGVFGGAPGGVVPDWLERADTLDA
LAGRLGLPAAEVAATVRRFNEHARAGTDPDFGRGDNAYDRFPGGRVLDPESVLSTLGPVETAPFYGVEVGSSALGTKGGP
RTDREGRVLDVDGDVIRGLYAAGNVMASPSGMVYGGAGATLAVAGVWGLYAGRAAARDPASGARSPVLSERSGS
>Mature_554_residues
MDEHETYDVVVLGSGAAGLTAALAAAVEGARVAVFEKAELLGGTTALSGGTIWVPANQPARAAGIKDSRADGLAYLASLS
NGMILPELAEALVDGGPELVDFVEQQTDLRFHLVEGYPDYHPERPGGLPRGGRSIEVGLFSFLGFEEWEDRIAGDTRRLL
IREMPLGGGTGALELDVLAEREKNRAEGLGRALVGGLLQACLRRGVHVATRSRGLRLVTGDGAVTGIEIATPDGTRSVRT
GAVVLATGGFEYDADLVRDFLRGPLTRAIGAPSNTGDGLRMAMRVGAQLGNMREAWWAPVVSLPGHRRDGARNGLLTARE
RALPGSIMVNGHGRRFANEAANYNAFGGAYHQLDASKFEYPNLPSYLIFDQSTVDRFGVFGGAPGGVVPDWLERADTLDA
LAGRLGLPAAEVAATVRRFNEHARAGTDPDFGRGDNAYDRFPGGRVLDPESVLSTLGPVETAPFYGVEVGSSALGTKGGP
RTDREGRVLDVDGDVIRGLYAAGNVMASPSGMVYGGAGATLAVAGVWGLYAGRAAARDPASGARSPVLSERSGS

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI71986328, Length=348, Percent_Identity=27.0114942528736, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 57971; Mature: 57971

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEHETYDVVVLGSGAAGLTAALAAAVEGARVAVFEKAELLGGTTALSGGTIWVPANQPA
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEHHHHHCCCEEECCCEEEEECCCCC
RAAGIKDSRADGLAYLASLSNGMILPELAEALVDGGPELVDFVEQQTDLRFHLVEGYPDY
HHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCC
HPERPGGLPRGGRSIEVGLFSFLGFEEWEDRIAGDTRRLLIREMPLGGGTGALELDVLAE
CCCCCCCCCCCCCEEEEHHHHHHCHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEHHHHH
REKNRAEGLGRALVGGLLQACLRRGVHVATRSRGLRLVTGDGAVTGIEIATPDGTRSVRT
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEEEECCCCCCCEEC
GAVVLATGGFEYDADLVRDFLRGPLTRAIGAPSNTGDGLRMAMRVGAQLGNMREAWWAPV
CEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
VSLPGHRRDGARNGLLTARERALPGSIMVNGHGRRFANEAANYNAFGGAYHQLDASKFEY
HCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCCHHHHCCCCCCCC
PNLPSYLIFDQSTVDRFGVFGGAPGGVVPDWLERADTLDALAGRLGLPAAEVAATVRRFN
CCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHARAGTDPDFGRGDNAYDRFPGGRVLDPESVLSTLGPVETAPFYGVEVGSSALGTKGGP
HHHCCCCCCCCCCCCCCHHCCCCCCEECHHHHHHHCCCCCCCCCCCEECCCHHCCCCCCC
RTDREGRVLDVDGDVIRGLYAAGNVMASPSGMVYGGAGATLAVAGVWGLYAGRAAARDPA
CCCCCCCEEECCHHHHHHHHHHCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCC
SGARSPVLSERSGS
CCCCCCCCCCCCCC
>Mature Secondary Structure
MDEHETYDVVVLGSGAAGLTAALAAAVEGARVAVFEKAELLGGTTALSGGTIWVPANQPA
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEHHHHHCCCEEECCCEEEEECCCCC
RAAGIKDSRADGLAYLASLSNGMILPELAEALVDGGPELVDFVEQQTDLRFHLVEGYPDY
HHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCC
HPERPGGLPRGGRSIEVGLFSFLGFEEWEDRIAGDTRRLLIREMPLGGGTGALELDVLAE
CCCCCCCCCCCCCEEEEHHHHHHCHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEHHHHH
REKNRAEGLGRALVGGLLQACLRRGVHVATRSRGLRLVTGDGAVTGIEIATPDGTRSVRT
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCEEEEEEECCCCCCCEEC
GAVVLATGGFEYDADLVRDFLRGPLTRAIGAPSNTGDGLRMAMRVGAQLGNMREAWWAPV
CEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
VSLPGHRRDGARNGLLTARERALPGSIMVNGHGRRFANEAANYNAFGGAYHQLDASKFEY
HCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCCHHHHCCCCCCCC
PNLPSYLIFDQSTVDRFGVFGGAPGGVVPDWLERADTLDALAGRLGLPAAEVAATVRRFN
CCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHARAGTDPDFGRGDNAYDRFPGGRVLDPESVLSTLGPVETAPFYGVEVGSSALGTKGGP
HHHCCCCCCCCCCCCCCHHCCCCCCEECHHHHHHHCCCCCCCCCCCEECCCHHCCCCCCC
RTDREGRVLDVDGDVIRGLYAAGNVMASPSGMVYGGAGATLAVAGVWGLYAGRAAARDPA
CCCCCCCEEECCHHHHHHHHHHCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCC
SGARSPVLSERSGS
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]