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The map label for this gene is mhpE [C]

Identifier: 158316508

GI number: 158316508

Start: 5664812

End: 5665822

Strand: Reverse

Name: mhpE [C]

Synonym: Franean1_4743

Alternate gene names: 158316508

Gene position: 5665822-5664812 (Counterclockwise)

Preceding gene: 158316509

Following gene: 158316507

Centisome position: 63.08

GC content: 72.21

Gene sequence:

>1011_bases
ATGAGCGGGCCGAACATCTATCTGCAGGACGTCACCCTGCGGGACGGCATGCACGCCATCCGGCACCGGGTCGACCCGGA
GCGGGTCGGCGCCATCGTGGCCGCTCTCGACAAGGCGGGCGTCCGGGCGATCGAGGTCACCCACGGCGACGGGCTGGCCG
GCTCCAGCCTGACGTACGGCCCCGGCAGCCACACGAACTGGGAGTGGATCGAGGCGGCGGTCACCAACGCCTCGCAGGCG
ACGATCACCACGCTGCTGCTACCGGGCGTGGGCACGATCGCCGAGCTGCGCCGCGCGCACGCGATGGGGGTCGGCTCGGT
CCGCGTCGCGACGCACTGCACCGAGGCGGACGTCGCCGCCCAGCACATCGCCGCGGCGCGCGAGCTCGGCATGGACGTCT
CCGGCTTCCTGATGATGAGCCACATGGCCGAGCCGGCCGAGCTGGCCGCCCAGGCCAAGCTGATGGAGTCGTACGGGGCG
CACTGTGTCTACGTCACCGACTCGGGCGGCCGGCTGACCACCGACCGCGTGCGCGAGCGGGTCCGTGCCTACCGCGACGT
CCTGCGCCCGGACACCCAGATCGGCATCCACGCGCACGAGAACCTCTCGCTGTCGGTCGCCAACTCGTTCGCCGCGGTCG
AGGAGGGCGCCTACCGCGTCGACGCGTCGCTCGCCGGCCAGGGAGCCGGCGCCGGCAACTGCCCGATCGAGCCGTTCGTC
GCGGTCGCGCTGCTGCTGGGCTGGGATCTCGACTGCGACCTGCTCGCGCTGGAGGACGCGGCCGAGGACCTGGTCCGGCC
GTTGCAGGACCGTCCGGTCCGGGTCGACCGCGAGACGCTCACGCTCGGCTTCGCGGGCGTGTACTCCAGTTTCCTCCGGC
ACGCCGAGATCGCCGCCGAGACCTACGGCGTGGACGCCCGCAGCATCCTGATCGAGGCGGGCCGGCGAAAGCTGGTCGGC
GGCCAGGAGGACATGCTCGTCGACATCGCCCTGGCGATACAGCCCAAGTAG

Upstream 100 bases:

>100_bases
GCGCCGCTCATTATCTGCCGGCCTACGCCGGCAACCTGGACATCATGACGTCGGCGGCCGTGCGGGTCGCCGAGCGCATC
GCTGGAGCCGAGGTGACGGC

Downstream 100 bases:

>100_bases
GCCCGCGACAACGGGCGGATGCCCGCAGGGCGCGTTCATCGCTCCGCCGTTTCCGCGTTTCCTTTTCTCTGTGTCATACC
CCCGCATGCTGCTTTTCGTG

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 2; 4-hydroxy-2-keto-pentanoic acid aldolase 2; 4-hydroxy-2-oxopentanoate aldolase 2

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSGPNIYLQDVTLRDGMHAIRHRVDPERVGAIVAALDKAGVRAIEVTHGDGLAGSSLTYGPGSHTNWEWIEAAVTNASQA
TITTLLLPGVGTIAELRRAHAMGVGSVRVATHCTEADVAAQHIAAARELGMDVSGFLMMSHMAEPAELAAQAKLMESYGA
HCVYVTDSGGRLTTDRVRERVRAYRDVLRPDTQIGIHAHENLSLSVANSFAAVEEGAYRVDASLAGQGAGAGNCPIEPFV
AVALLLGWDLDCDLLALEDAAEDLVRPLQDRPVRVDRETLTLGFAGVYSSFLRHAEIAAETYGVDARSILIEAGRRKLVG
GQEDMLVDIALAIQPK

Sequences:

>Translated_336_residues
MSGPNIYLQDVTLRDGMHAIRHRVDPERVGAIVAALDKAGVRAIEVTHGDGLAGSSLTYGPGSHTNWEWIEAAVTNASQA
TITTLLLPGVGTIAELRRAHAMGVGSVRVATHCTEADVAAQHIAAARELGMDVSGFLMMSHMAEPAELAAQAKLMESYGA
HCVYVTDSGGRLTTDRVRERVRAYRDVLRPDTQIGIHAHENLSLSVANSFAAVEEGAYRVDASLAGQGAGAGNCPIEPFV
AVALLLGWDLDCDLLALEDAAEDLVRPLQDRPVRVDRETLTLGFAGVYSSFLRHAEIAAETYGVDARSILIEAGRRKLVG
GQEDMLVDIALAIQPK
>Mature_335_residues
SGPNIYLQDVTLRDGMHAIRHRVDPERVGAIVAALDKAGVRAIEVTHGDGLAGSSLTYGPGSHTNWEWIEAAVTNASQAT
ITTLLLPGVGTIAELRRAHAMGVGSVRVATHCTEADVAAQHIAAARELGMDVSGFLMMSHMAEPAELAAQAKLMESYGAH
CVYVTDSGGRLTTDRVRERVRAYRDVLRPDTQIGIHAHENLSLSVANSFAAVEEGAYRVDASLAGQGAGAGNCPIEPFVA
VALLLGWDLDCDLLALEDAAEDLVRPLQDRPVRVDRETLTLGFAGVYSSFLRHAEIAAETYGVDARSILIEAGRRKLVGG
QEDMLVDIALAIQPK

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=334, Percent_Identity=64.3712574850299, Blast_Score=412, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA2_FRASN (A8LEP8)

Other databases:

- EMBL:   CP000820
- RefSeq:   YP_001509016.1
- ProteinModelPortal:   A8LEP8
- SMR:   A8LEP8
- GeneID:   5673085
- GenomeReviews:   CP000820_GR
- KEGG:   fre:Franean1_4743
- HOGENOM:   HBG300122
- OMA:   TTRLFIQ
- ProtClustDB:   PRK08195
- BioCyc:   FSP1855:FRANEAN1_4743-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 35808; Mature: 35677

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 168-168 BINDING 197-197 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPNIYLQDVTLRDGMHAIRHRVDPERVGAIVAALDKAGVRAIEVTHGDGLAGSSLTYG
CCCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECC
PGSHTNWEWIEAAVTNASQATITTLLLPGVGTIAELRRAHAMGVGSVRVATHCTEADVAA
CCCCCCHHHHHHHHCCCCHHHEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHH
QHIAAARELGMDVSGFLMMSHMAEPAELAAQAKLMESYGAHCVYVTDSGGRLTTDRVRER
HHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCEECHHHHHHH
VRAYRDVLRPDTQIGIHAHENLSLSVANSFAAVEEGAYRVDASLAGQGAGAGNCPIEPFV
HHHHHHHHCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHH
AVALLLGWDLDCDLLALEDAAEDLVRPLQDRPVRVDRETLTLGFAGVYSSFLRHAEIAAE
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCEECCHHEEEEHHHHHHHHHHHHHHHHH
TYGVDARSILIEAGRRKLVGGQEDMLVDIALAIQPK
HCCCCHHHHHHHHCCHHCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure 
SGPNIYLQDVTLRDGMHAIRHRVDPERVGAIVAALDKAGVRAIEVTHGDGLAGSSLTYG
CCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEECC
PGSHTNWEWIEAAVTNASQATITTLLLPGVGTIAELRRAHAMGVGSVRVATHCTEADVAA
CCCCCCHHHHHHHHCCCCHHHEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHH
QHIAAARELGMDVSGFLMMSHMAEPAELAAQAKLMESYGAHCVYVTDSGGRLTTDRVRER
HHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCEECHHHHHHH
VRAYRDVLRPDTQIGIHAHENLSLSVANSFAAVEEGAYRVDASLAGQGAGAGNCPIEPFV
HHHHHHHHCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHH
AVALLLGWDLDCDLLALEDAAEDLVRPLQDRPVRVDRETLTLGFAGVYSSFLRHAEIAAE
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCEECCHHEEEEHHHHHHHHHHHHHHHHH
TYGVDARSILIEAGRRKLVGGQEDMLVDIALAIQPK
HCCCCHHHHHHHHCCHHCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA