BacMap

Definition	Frankia sp. EAN1pec chromosome, complete genome.
Accession	NC_009921
Length	8,982,042

Click here to switch to the map view.

The map label for this gene is limC [H]

Identifier: 158316264

GI number: 158316264

Start: 5356385

End: 5357224

Strand: Reverse

Name: limC [H]

Synonym: Franean1_4489

Alternate gene names: 158316264

Gene position: 5357224-5356385 (Counterclockwise)

Preceding gene: 158316265

Following gene: 158316262

Centisome position: 59.64

GC content: 69.05

Gene sequence:

>840_bases
ATGGCCGGGCGAGTCGCCGGGAAGGTCGCGCTGATCACCGGGGCGGCGCGCGGGCAGGGCCGCAGCCACGCGGTTCGGCT
CGCGCAGGAGGGGGCCGACATCATCGCCGTCGACCTCTGCGCCGACGTGCCGGGCGTGCCGTACCCGGGGGGCACCCGCG
AGGATCTGGCCGAGACGGTACGGCAGGTGGAGGCCCTCGATCGGCGGGCCGTGGCGACGGTCGCCGACGTGCGTGACCAC
GAGCAGCTCGCGGCGGCTGTGGTGGGGGGTGTCGCCGAGTTCGGGCGGCTCGACGTGGTCAGCGCGAACGCGGGCATCGC
CATGCCGCCCTTCCCCACCCACGAGATGCCCGAGGAGGTGTGGCAGGGCATGCTTGCGGTCAACCTGACCGGCGTCTGGC
ACACCTGCAAGGCCGCCATACCGCACCTGATCGCGGGTGGCCGCGGCGGGTCGATCATCCTTACGAGTTCCGCGGCTGGT
CTCAGGGGTTACGAGAACATCGCCAACTACGTCGCGGCCAAGCACGGCGTGGTCGGTCTGATGCGGACGCTGGCCAACGA
GCTCGCCCGGCACTCGATCCGGGTGAATTCGGTGCATCCCACCACTGTCTCGACCGAGATGATCCAGAACGAGTCGACCT
ACCGCCAGTTCCGGCCGGACCTGACCGACACGCCGACCGAGGACGACGTGCGCGACGCGTTCACGTCGCTCAATCTGATA
CCGGTGCCCTGGATTGAGTCGATTGACGTGTCGAACGCGCTGCTGTTTCTCGCGTCCGACGAGTCTCGGTACATCACCGG
CATCACGCTGCCGATCGACGCCGGCCAGATGGTCAAGTAG

Upstream 100 bases:

>100_bases
TCGATCCCGGCCGCAGCACGCTACGCGTCCATGTCGAAGAACAGGCGAGTCAGCCCGGCGGTGGCGATGGTCCGGTGGGC
GCCGACCAGGGGAGCTTCTG

Downstream 100 bases:

>100_bases
GACCCGTGGGGGCTGGGCGCGGGCGGCCTCGTCACCCGGATGCTCATCCCGGGGACTCAGCCGCCGAGATGACGCTCGCA
TCGATCGGCGAAGGTTCAGC

Product: short-chain dehydrogenase/reductase SDR

Products: @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate!; NADH. [C]

Alternate protein names: (4R,6S)-carveol dehydrogenase; CDH; Carveol dehydrogenase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAGRVAGKVALITGAARGQGRSHAVRLAQEGADIIAVDLCADVPGVPYPGGTREDLAETVRQVEALDRRAVATVADVRDH
EQLAAAVVGGVAEFGRLDVVSANAGIAMPPFPTHEMPEEVWQGMLAVNLTGVWHTCKAAIPHLIAGGRGGSIILTSSAAG
LRGYENIANYVAAKHGVVGLMRTLANELARHSIRVNSVHPTTVSTEMIQNESTYRQFRPDLTDTPTEDDVRDAFTSLNLI
PVPWIESIDVSNALLFLASDESRYITGITLPIDAGQMVK

Sequences:

>Translated_279_residues
MAGRVAGKVALITGAARGQGRSHAVRLAQEGADIIAVDLCADVPGVPYPGGTREDLAETVRQVEALDRRAVATVADVRDH
EQLAAAVVGGVAEFGRLDVVSANAGIAMPPFPTHEMPEEVWQGMLAVNLTGVWHTCKAAIPHLIAGGRGGSIILTSSAAG
LRGYENIANYVAAKHGVVGLMRTLANELARHSIRVNSVHPTTVSTEMIQNESTYRQFRPDLTDTPTEDDVRDAFTSLNLI
PVPWIESIDVSNALLFLASDESRYITGITLPIDAGQMVK
>Mature_278_residues
AGRVAGKVALITGAARGQGRSHAVRLAQEGADIIAVDLCADVPGVPYPGGTREDLAETVRQVEALDRRAVATVADVRDHE
QLAAAVVGGVAEFGRLDVVSANAGIAMPPFPTHEMPEEVWQGMLAVNLTGVWHTCKAAIPHLIAGGRGGSIILTSSAAGL
RGYENIANYVAAKHGVVGLMRTLANELARHSIRVNSVHPTTVSTEMIQNESTYRQFRPDLTDTPTEDDVRDAFTSLNLIP
VPWIESIDVSNALLFLASDESRYITGITLPIDAGQMVK

Specific function: Catalyzes the oxidation of carveol to carvone, with a strong stereoselectivity since it efficiently converts only the (6S)-stereoisomers, of which (-)-(4R,6S)-trans-carveol is the better substrate. Displays a broad substrate specificity with a preference

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=284, Percent_Identity=34.5070422535211, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4504505, Length=219, Percent_Identity=34.703196347032, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI7705925, Length=272, Percent_Identity=31.9852941176471, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI31542939, Length=271, Percent_Identity=30.6273062730627, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI32483357, Length=274, Percent_Identity=29.9270072992701, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI40254992, Length=280, Percent_Identity=28.9285714285714, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI40254986, Length=204, Percent_Identity=31.8627450980392, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI224922801, Length=189, Percent_Identity=33.8624338624339, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI66933014, Length=277, Percent_Identity=28.158844765343, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4758504, Length=216, Percent_Identity=31.9444444444444, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI5031737, Length=270, Percent_Identity=28.1481481481481, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI19923817, Length=284, Percent_Identity=24.2957746478873, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI126723750, Length=274, Percent_Identity=27.3722627737226, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI59889578, Length=206, Percent_Identity=28.6407766990291, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI210032110, Length=243, Percent_Identity=26.3374485596708, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI126723191, Length=198, Percent_Identity=32.3232323232323, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI83715985, Length=197, Percent_Identity=32.48730964467, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI142976729, Length=214, Percent_Identity=27.1028037383178, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI33667109, Length=206, Percent_Identity=29.6116504854369, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789208, Length=274, Percent_Identity=33.2116788321168, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082100, Length=277, Percent_Identity=32.4909747292419, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787335, Length=270, Percent_Identity=32.2222222222222, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1790717, Length=277, Percent_Identity=31.4079422382672, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1788459, Length=272, Percent_Identity=28.3088235294118, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787905, Length=273, Percent_Identity=26.007326007326, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789378, Length=271, Percent_Identity=28.4132841328413, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082160, Length=280, Percent_Identity=26.7857142857143, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787526, Length=275, Percent_Identity=27.6363636363636, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI2367175, Length=272, Percent_Identity=27.2058823529412, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789057, Length=274, Percent_Identity=29.5620437956204, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786812, Length=273, Percent_Identity=26.3736263736264, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=236, Percent_Identity=31.7796610169492, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17561402, Length=282, Percent_Identity=29.4326241134752, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=214, Percent_Identity=33.1775700934579, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17560676, Length=272, Percent_Identity=27.2058823529412, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115534694, Length=272, Percent_Identity=30.1470588235294, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=287, Percent_Identity=29.9651567944251, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=277, Percent_Identity=27.7978339350181, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI193203117, Length=254, Percent_Identity=25.1968503937008, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=279, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=209, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI193203115, Length=253, Percent_Identity=25.296442687747, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=282, Percent_Identity=27.3049645390071, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=280, Percent_Identity=26.7857142857143, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=280, Percent_Identity=27.8571428571429, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=273, Percent_Identity=27.1062271062271, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71982365, Length=205, Percent_Identity=30.7317073170732, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=212, Percent_Identity=27.3584905660377, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=280, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=284, Percent_Identity=27.112676056338, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562990, Length=278, Percent_Identity=26.2589928057554, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=208, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=232, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=278, Percent_Identity=25.8992805755396, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=283, Percent_Identity=27.208480565371, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=291, Percent_Identity=26.8041237113402, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=289, Percent_Identity=25.9515570934256, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=215, Percent_Identity=33.0232558139535, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324126, Length=226, Percent_Identity=26.1061946902655, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI18859837, Length=220, Percent_Identity=32.2727272727273, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644339, Length=278, Percent_Identity=28.0575539568345, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28571526, Length=277, Percent_Identity=28.5198555956679, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640492, Length=210, Percent_Identity=30.952380952381, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI23397609, Length=274, Percent_Identity=29.9270072992701, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21357041, Length=274, Percent_Identity=28.8321167883212, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355319, Length=265, Percent_Identity=27.1698113207547, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364892, Length=211, Percent_Identity=30.3317535545024, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24649181, Length=232, Percent_Identity=33.1896551724138, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24639444, Length=275, Percent_Identity=26.9090909090909, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357673, Length=205, Percent_Identity=30.2439024390244, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737361, Length=215, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644337, Length=198, Percent_Identity=30.3030303030303, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24581726, Length=209, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665243, Length=222, Percent_Identity=28.8288288288288, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221331218, Length=222, Percent_Identity=28.8288288288288, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648944, Length=239, Percent_Identity=26.7782426778243, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125 [C]

Molecular weight: Translated: 29634; Mature: 29503

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRVAGKVALITGAARGQGRSHAVRLAQEGADIIAVDLCADVPGVPYPGGTREDLAETV
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
RQVEALDRRAVATVADVRDHEQLAAAVVGGVAEFGRLDVVSANAGIAMPPFPTHEMPEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHH
WQGMLAVNLTGVWHTCKAAIPHLIAGGRGGSIILTSSAAGLRGYENIANYVAAKHGVVGL
HCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
MRTLANELARHSIRVNSVHPTTVSTEMIQNESTYRQFRPDLTDTPTEDDVRDAFTSLNLI
HHHHHHHHHHHCEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCE
PVPWIESIDVSNALLFLASDESRYITGITLPIDAGQMVK
ECCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
AGRVAGKVALITGAARGQGRSHAVRLAQEGADIIAVDLCADVPGVPYPGGTREDLAETV
CCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
RQVEALDRRAVATVADVRDHEQLAAAVVGGVAEFGRLDVVSANAGIAMPPFPTHEMPEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHH
WQGMLAVNLTGVWHTCKAAIPHLIAGGRGGSIILTSSAAGLRGYENIANYVAAKHGVVGL
HCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
MRTLANELARHSIRVNSVHPTTVSTEMIQNESTYRQFRPDLTDTPTEDDVRDAFTSLNLI
HHHHHHHHHHHCEEECCCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCE
PVPWIESIDVSNALLFLASDESRYITGITLPIDAGQMVK
ECCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 15.4 {2-deoxygluconate}} [C]

Substrates: @DEOX03.txt*2-Deoxy-D-Gluconate!; NAD(+) [C]

Specific reaction: @DEOX03.txt*2-Deoxy-D-Gluconate! + NAD(+) = @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10473585 [H]