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The map label for this gene is pckG [H]

Identifier: 158316160

GI number: 158316160

Start: 5227800

End: 5229218

Strand: Reverse

Name: pckG [H]

Synonym: Franean1_4382

Alternate gene names: 158316160

Gene position: 5229218-5227800 (Counterclockwise)

Preceding gene: 158316164

Following gene: 158316159

Centisome position: 58.22

GC content: 68.08

Gene sequence:

>1419_bases
ATGCCCGTCACGATCCCGGGCCTACAGCCGACGCCGACGACACACCCGGCACTGTTGGAGTGGGTCGCCACGATCGCCGA
CCTCACCCGGCCCGACCGGGTTCACTGGTGCGACGGCAGCGACGCCGAGTACGACCAGCTCTGCGCGGAGCTCGTCGACA
AGGGCACGTTCCTCCGTCTCGCCGAGGACAAGCGGCCCGGCAGCTACTACGCCGCGAGCGACCCCAGCGACGTCGCCCGC
GTCGAGGACCGCACCTTCATCTGCTCGAGGAGCCAGGACGACGCCGGTCCGACGAACAACTGGACCGACCCGGACGAGAT
GCGCATCACCCTGCGGGGCCTGTTCGCGGGTTGCATGCGGGGCCGCACCATGTACGTCGTCCCGTTCTGCATGGGATCGC
TAGGCTCACCGATCTCCGCACTCGGCGTCGAGATCACCGACTCGGCCTACGTCGCGGTCTCGATGCGTGTAATGACCCGA
ATGGGCCAACCGGCACTCGACCAGCTCGGACAGGACGGCTTCTTCGTCCCCGCCGTGCACAGCGTCGGCGCGCCGCGCCA
GCCCGAGCAACCCGACGTCGCCTGGCCCTGCAACGCCACCAAGTACATCGTCCACTTCCCCGAGACACGAGAAATCTGGA
GCTACGGCTCCGGCTACGGCGGCAACGCCCTGCTCGGCAAGAAGTACTACGCGCTACGGATCGCCTCGGTGATGGCCCGC
GACGACGGCTGGCTCGCCGAGCACATGCTGATCCTCAAGCTCACCGGACCCGACGGGAACACCCATTACATCGCGGCCGG
CTTTCCGAGCGCCTGCGGCAAAACCAACCTCGCCATGCTCGTCCCGACCATCCCCGGCTGGAAGGTCGAGACCATCGGGG
ACGACATCGCCTGGATGCGCTTCGGAGACGACGGACGGCTCTACGCCGTCAACCCCGAGGCCGGCTTCTTCGGCGTCGCG
CCGGGCACCGGCCGGACGACCAACCCCAACGCCATCGACACGATCCACAGCAATGCGATCTTTACGAATGTCGCGCGCAC
CGATGACGGAGACGTGTGGTGGGAAGGGCTGACCAAGGAACCCCCGGCACATCTCATCGACTGGCAGGGCCGCGACTGGA
CACCACAGTCCGCGACGCCGGCCGCGCATCCCAACGCCCGTTTCACCGCCCCCGCCAGCCAATGCCCGACGATCGCTGCG
GAATGGGCCGGCCCGGCGGGCGTTCCGATCTCCGTTGACTGTGCCGCCTGGGAACACGAAAACCGACACGATCCGTCGAT
ACTTCACCGACCTCGGCCCCCGCATGCCCGACGCTCTCTGGGTCGAACTCGCGGCCCTCGCCGACCGGCTGCGCTGACAC
CGTCTCCCACGCCTCACACCCTCCGTGAGCCAACGGGTTCGGCCGCCGCCACAGCCTGA

Upstream 100 bases:

>100_bases
CGACGACATGTTGACGATCTTTAGGGTCGATAACCTGACAACTTCGTCAGATAGACGAGGTACAGTTCAACCATCCGACA
GTCTGTCGAAGGAGTGGGAG

Downstream 100 bases:

>100_bases
CAGCAATGCTGCCGACCGACGCCGTCCACACCCCTGCCCCCGGTGCGCCTAGCTAGCTGTGTCCTGTCGGCAGCATTGGT
TTGGCCACGCCGTCTTCCAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MPVTIPGLQPTPTTHPALLEWVATIADLTRPDRVHWCDGSDAEYDQLCAELVDKGTFLRLAEDKRPGSYYAASDPSDVAR
VEDRTFICSRSQDDAGPTNNWTDPDEMRITLRGLFAGCMRGRTMYVVPFCMGSLGSPISALGVEITDSAYVAVSMRVMTR
MGQPALDQLGQDGFFVPAVHSVGAPRQPEQPDVAWPCNATKYIVHFPETREIWSYGSGYGGNALLGKKYYALRIASVMAR
DDGWLAEHMLILKLTGPDGNTHYIAAGFPSACGKTNLAMLVPTIPGWKVETIGDDIAWMRFGDDGRLYAVNPEAGFFGVA
PGTGRTTNPNAIDTIHSNAIFTNVARTDDGDVWWEGLTKEPPAHLIDWQGRDWTPQSATPAAHPNARFTAPASQCPTIAA
EWAGPAGVPISVDCAAWEHENRHDPSILHRPRPPHARRSLGRTRGPRRPAALTPSPTPHTLREPTGSAAATA

Sequences:

>Translated_472_residues
MPVTIPGLQPTPTTHPALLEWVATIADLTRPDRVHWCDGSDAEYDQLCAELVDKGTFLRLAEDKRPGSYYAASDPSDVAR
VEDRTFICSRSQDDAGPTNNWTDPDEMRITLRGLFAGCMRGRTMYVVPFCMGSLGSPISALGVEITDSAYVAVSMRVMTR
MGQPALDQLGQDGFFVPAVHSVGAPRQPEQPDVAWPCNATKYIVHFPETREIWSYGSGYGGNALLGKKYYALRIASVMAR
DDGWLAEHMLILKLTGPDGNTHYIAAGFPSACGKTNLAMLVPTIPGWKVETIGDDIAWMRFGDDGRLYAVNPEAGFFGVA
PGTGRTTNPNAIDTIHSNAIFTNVARTDDGDVWWEGLTKEPPAHLIDWQGRDWTPQSATPAAHPNARFTAPASQCPTIAA
EWAGPAGVPISVDCAAWEHENRHDPSILHRPRPPHARRSLGRTRGPRRPAALTPSPTPHTLREPTGSAAATA
>Mature_471_residues
PVTIPGLQPTPTTHPALLEWVATIADLTRPDRVHWCDGSDAEYDQLCAELVDKGTFLRLAEDKRPGSYYAASDPSDVARV
EDRTFICSRSQDDAGPTNNWTDPDEMRITLRGLFAGCMRGRTMYVVPFCMGSLGSPISALGVEITDSAYVAVSMRVMTRM
GQPALDQLGQDGFFVPAVHSVGAPRQPEQPDVAWPCNATKYIVHFPETREIWSYGSGYGGNALLGKKYYALRIASVMARD
DGWLAEHMLILKLTGPDGNTHYIAAGFPSACGKTNLAMLVPTIPGWKVETIGDDIAWMRFGDDGRLYAVNPEAGFFGVAP
GTGRTTNPNAIDTIHSNAIFTNVARTDDGDVWWEGLTKEPPAHLIDWQGRDWTPQSATPAAHPNARFTAPASQCPTIAAE
WAGPAGVPISVDCAAWEHENRHDPSILHRPRPPHARRSLGRTRGPRRPAALTPSPTPHTLREPTGSAAATA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=408, Percent_Identity=52.4509803921569, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI66346721, Length=407, Percent_Identity=53.5626535626536, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI66346723, Length=369, Percent_Identity=53.929539295393, Blast_Score=387, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71989649, Length=422, Percent_Identity=49.7630331753554, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71989645, Length=422, Percent_Identity=49.7630331753554, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71989658, Length=422, Percent_Identity=49.7630331753554, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32564411, Length=418, Percent_Identity=50, Blast_Score=413, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17555492, Length=418, Percent_Identity=50, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71989653, Length=396, Percent_Identity=51.2626262626263, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32564407, Length=325, Percent_Identity=55.6923076923077, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32564409, Length=171, Percent_Identity=56.7251461988304, Blast_Score=202, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17540956, Length=204, Percent_Identity=31.3725490196078, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24655082, Length=409, Percent_Identity=52.5672371638142, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24655085, Length=398, Percent_Identity=54.5226130653266, Blast_Score=428, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161077226, Length=374, Percent_Identity=54.0106951871658, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655088, Length=310, Percent_Identity=59.0322580645161, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 51270; Mature: 51139

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVTIPGLQPTPTTHPALLEWVATIADLTRPDRVHWCDGSDAEYDQLCAELVDKGTFLRL
CCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEE
AEDKRPGSYYAASDPSDVARVEDRTFICSRSQDDAGPTNNWTDPDEMRITLRGLFAGCMR
ECCCCCCCEECCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHEEHHHHHHHHHHC
GRTMYVVPFCMGSLGSPISALGVEITDSAYVAVSMRVMTRMGQPALDQLGQDGFFVPAVH
CCEEEEEEEHHCCCCCCHHHHCEEECCCEEEEHHHHHHHHCCCHHHHHCCCCCEEEEEHH
SVGAPRQPEQPDVAWPCNATKYIVHFPETREIWSYGSGYGGNALLGKKYYALRIASVMAR
HCCCCCCCCCCCEECCCCCCEEEEECCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHC
DDGWLAEHMLILKLTGPDGNTHYIAAGFPSACGKTNLAMLVPTIPGWKVETIGDDIAWMR
CCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCEEEEE
FGDDGRLYAVNPEAGFFGVAPGTGRTTNPNAIDTIHSNAIFTNVARTDDGDVWWEGLTKE
ECCCCEEEEECCCCCEEECCCCCCCCCCCCHHHEECCCEEEEEECCCCCCCEEECCCCCC
PPAHLIDWQGRDWTPQSATPAAHPNARFTAPASQCPTIAAEWAGPAGVPISVDCAAWEHE
CCCCEEECCCCCCCCCCCCCCCCCCCEEECCHHHCCCEEECCCCCCCCEEEEEEECCCCC
NRHDPSILHRPRPPHARRSLGRTRGPRRPAALTPSPTPHTLREPTGSAAATA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVTIPGLQPTPTTHPALLEWVATIADLTRPDRVHWCDGSDAEYDQLCAELVDKGTFLRL
CEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEE
AEDKRPGSYYAASDPSDVARVEDRTFICSRSQDDAGPTNNWTDPDEMRITLRGLFAGCMR
ECCCCCCCEECCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHEEHHHHHHHHHHC
GRTMYVVPFCMGSLGSPISALGVEITDSAYVAVSMRVMTRMGQPALDQLGQDGFFVPAVH
CCEEEEEEEHHCCCCCCHHHHCEEECCCEEEEHHHHHHHHCCCHHHHHCCCCCEEEEEHH
SVGAPRQPEQPDVAWPCNATKYIVHFPETREIWSYGSGYGGNALLGKKYYALRIASVMAR
HCCCCCCCCCCCEECCCCCCEEEEECCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHC
DDGWLAEHMLILKLTGPDGNTHYIAAGFPSACGKTNLAMLVPTIPGWKVETIGDDIAWMR
CCCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCEEEEE
FGDDGRLYAVNPEAGFFGVAPGTGRTTNPNAIDTIHSNAIFTNVARTDDGDVWWEGLTKE
ECCCCEEEEECCCCCEEECCCCCCCCCCCCHHHEECCCEEEEEECCCCCCCEEECCCCCC
PPAHLIDWQGRDWTPQSATPAAHPNARFTAPASQCPTIAAEWAGPAGVPISVDCAAWEHE
CCCCEEECCCCCCCCCCCCCCCCCCCEEECCHHHCCCEEECCCCCCCCEEEEEEECCCCC
NRHDPSILHRPRPPHARRSLGRTRGPRRPAALTPSPTPHTLREPTGSAAATA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA