BacMap

Definition	Frankia sp. EAN1pec chromosome, complete genome.
Accession	NC_009921
Length	8,982,042

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The map label for this gene is menE [H]

Identifier: 158316087

GI number: 158316087

Start: 5143111

End: 5144514

Strand: Reverse

Name: menE [H]

Synonym: Franean1_4305

Alternate gene names: 158316087

Gene position: 5144514-5143111 (Counterclockwise)

Preceding gene: 158316089

Following gene: 158316086

Centisome position: 57.28

GC content: 73.08

Gene sequence:

>1404_bases
GTGAGTGACCGCCCCGCGCTCGTCTTCGAGGAACAGCGGTACAGCCTCGAGCGACTCGACGCGCTGACGGACGGGCTGGC
CACGGCGTTGGCGAAACATGGGGTCCGGGCGGGGGAACGCGTCGCGCTGATGGCCTCGAACCGGCCGGAGTTCGTACTGG
CCGTCCGGGCTGTCTGGCGACTGGGTGCGGCCATCGTACTGCTGAGCCCGTCCTGGCGGCGGGCCGAGATCGAACACGCG
CTGGCCGTCACCGAGCCCGCGCATGCCATCGGTGACCAGGACGCGCTGGCCGAGCTGCTGCCGATGTGGCACCTCGACGA
GCCGATCGGCGCGGGGGAGGGGCCGTTCCCGACACCGGACCCGCACGCCGACGCGCTACTGGTCTTCAGCTCCGGCACGA
CCGGCCTGCCGAAGGCCGCCCGGCACACGCGGGCCTCGTTCGGCGCGGCCGTCGGGCACTGGCGCGACGCACTCGGCCTC
ACCGCCGCGGACCGCGTCCAGATCACCACCCCGCCCTCGCACATCCTCGGCCTGCTCAACATCGTCACCGCGCTGGAGAC
CGGCGCCTGGATGCGGCTGCACCGCCGGTTCGACCTCGAGCTGATGCTGCGCTCGATCGAGACCGACCGGATCACCGTCG
AGATGGCCGTCGCGCCGATCGCGCTGGCCATCGCCGCCCACCCCACGCTGGAGTCCTTCGACCTGTCGTCGCTGCGGTAC
ATCATGTGGGGCGCGACACCGGTGACGGCCAGCGTCGCCGAGACCGTCACCAGGCGCACGGGCGTCGGCTGGGTGCCCGC
CTACGGGGCCAGTGAGCTGCCGGTCATCGCCTGCAACCCGCTCGCGGGCGCCCGGCTCGACAGCGTCGGCCGCGCCGTGC
CGGGCGTGCGGCTGCGGGTGGTGTCGCTGGAGAGCGGAGCGCCGGTCGGCCCCGGCGAGACCGGCGAGATCCAGGCCCGC
GCCGAGTCGCTGATGGCCGGCTACCTGCCCGCCGAGGCGACGGCGGACGCGTTCGACGACGGCTGGTACCGCACCGGCGA
CGTCGGCAACCTCGACGCGGACGGGTGGCTCCGGCTCACCGACCGGGTCAAGGAGATGGTCAAGGTGCGCGGCTTCCAGG
TCGCGCCCGCCGAGGTCGAGGGTGTGCTGCACGCGCACCCCGCGGTCGCCGACTGCGCCGTGTTCGGCCTCCCCGACACG
GCCGACGGCGAGGTGCTCGTCGCGGCCGTCGTGACGAGTACCCAGGTCGACCCGGCGGAGCTCGTCGCGTGGGTGGGCGA
ACGGCTCGCCTCCTACAAGCGGCCCCGCCGGATCGAGCTCGTGGAAGAAATCCCCCGCCTGCCCTCGGGCAAGGTACTGC
GGCGAGTCCTCAAGGAGCGCTATGGACGTACGTCTGACGACTGA

Upstream 100 bases:

>100_bases
GCTGACATGAGAATATAATTTCCGCATGCGAACCGGGGAGGTTCGCGACGCCGTCCCGTGCCTGCCTCCGCGTTGACGTG
ACCGTCCGAAAGGAGCGGCA

Downstream 100 bases:

>100_bases
GCAGCGGGAGCTGCGCGAGGCGGCCGCCCGGCTGGCCGACGACCTCGGCCCGGGATCGGTCATCGATCTCGACGACGCCG
ACCGCGCGGCGCGGCTGGAG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSDRPALVFEEQRYSLERLDALTDGLATALAKHGVRAGERVALMASNRPEFVLAVRAVWRLGAAIVLLSPSWRRAEIEHA
LAVTEPAHAIGDQDALAELLPMWHLDEPIGAGEGPFPTPDPHADALLVFSSGTTGLPKAARHTRASFGAAVGHWRDALGL
TAADRVQITTPPSHILGLLNIVTALETGAWMRLHRRFDLELMLRSIETDRITVEMAVAPIALAIAAHPTLESFDLSSLRY
IMWGATPVTASVAETVTRRTGVGWVPAYGASELPVIACNPLAGARLDSVGRAVPGVRLRVVSLESGAPVGPGETGEIQAR
AESLMAGYLPAEATADAFDDGWYRTGDVGNLDADGWLRLTDRVKEMVKVRGFQVAPAEVEGVLHAHPAVADCAVFGLPDT
ADGEVLVAAVVTSTQVDPAELVAWVGERLASYKRPRRIELVEEIPRLPSGKVLRRVLKERYGRTSDD

Sequences:

>Translated_467_residues
MSDRPALVFEEQRYSLERLDALTDGLATALAKHGVRAGERVALMASNRPEFVLAVRAVWRLGAAIVLLSPSWRRAEIEHA
LAVTEPAHAIGDQDALAELLPMWHLDEPIGAGEGPFPTPDPHADALLVFSSGTTGLPKAARHTRASFGAAVGHWRDALGL
TAADRVQITTPPSHILGLLNIVTALETGAWMRLHRRFDLELMLRSIETDRITVEMAVAPIALAIAAHPTLESFDLSSLRY
IMWGATPVTASVAETVTRRTGVGWVPAYGASELPVIACNPLAGARLDSVGRAVPGVRLRVVSLESGAPVGPGETGEIQAR
AESLMAGYLPAEATADAFDDGWYRTGDVGNLDADGWLRLTDRVKEMVKVRGFQVAPAEVEGVLHAHPAVADCAVFGLPDT
ADGEVLVAAVVTSTQVDPAELVAWVGERLASYKRPRRIELVEEIPRLPSGKVLRRVLKERYGRTSDD
>Mature_466_residues
SDRPALVFEEQRYSLERLDALTDGLATALAKHGVRAGERVALMASNRPEFVLAVRAVWRLGAAIVLLSPSWRRAEIEHAL
AVTEPAHAIGDQDALAELLPMWHLDEPIGAGEGPFPTPDPHADALLVFSSGTTGLPKAARHTRASFGAAVGHWRDALGLT
AADRVQITTPPSHILGLLNIVTALETGAWMRLHRRFDLELMLRSIETDRITVEMAVAPIALAIAAHPTLESFDLSSLRYI
MWGATPVTASVAETVTRRTGVGWVPAYGASELPVIACNPLAGARLDSVGRAVPGVRLRVVSLESGAPVGPGETGEIQARA
ESLMAGYLPAEATADAFDDGWYRTGDVGNLDADGWLRLTDRVKEMVKVRGFQVAPAEVEGVLHAHPAVADCAVFGLPDTA
DGEVLVAAVVTSTQVDPAELVAWVGERLASYKRPRRIELVEEIPRLPSGKVLRRVLKERYGRTSDD

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=507, Percent_Identity=26.8244575936884, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI187761345, Length=523, Percent_Identity=29.6367112810707, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI187761343, Length=523, Percent_Identity=29.6367112810707, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI38505220, Length=352, Percent_Identity=30.1136363636364, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI122937307, Length=364, Percent_Identity=27.1978021978022, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI157311624, Length=476, Percent_Identity=26.6806722689076, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI157311622, Length=476, Percent_Identity=26.6806722689076, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI115511026, Length=499, Percent_Identity=26.0521042084168, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI156151445, Length=525, Percent_Identity=24.3809523809524, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI58082049, Length=358, Percent_Identity=27.6536312849162, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI62865631, Length=516, Percent_Identity=23.6434108527132, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI13325055, Length=516, Percent_Identity=23.6434108527132, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI28416953, Length=530, Percent_Identity=24.9056603773585, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40807491, Length=503, Percent_Identity=22.0675944333996, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI8923896, Length=124, Percent_Identity=35.4838709677419, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI253970489, Length=124, Percent_Identity=35.4838709677419, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=27.5529865125241, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI145693145, Length=508, Percent_Identity=28.740157480315, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI221142682, Length=490, Percent_Identity=25.3061224489796, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786810, Length=503, Percent_Identity=26.0437375745527, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789201, Length=355, Percent_Identity=28.7323943661972, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788595, Length=464, Percent_Identity=26.7241379310345, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790505, Length=365, Percent_Identity=24.6575342465753, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786801, Length=418, Percent_Identity=24.6411483253589, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786529, Length=376, Percent_Identity=25.7978723404255, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17557194, Length=509, Percent_Identity=30.2554027504912, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17531443, Length=365, Percent_Identity=33.4246575342466, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17538037, Length=482, Percent_Identity=29.253112033195, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=32.6923076923077, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32563687, Length=461, Percent_Identity=28.8503253796095, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17558820, Length=467, Percent_Identity=28.6937901498929, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71985884, Length=348, Percent_Identity=29.3103448275862, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25141359, Length=476, Percent_Identity=26.2605042016807, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17560140, Length=480, Percent_Identity=25.2083333333333, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71996755, Length=489, Percent_Identity=25.1533742331288, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=356, Percent_Identity=28.0898876404494, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=21.9924812030075, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=21.9924812030075, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=21.9924812030075, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115534440, Length=533, Percent_Identity=22.5140712945591, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17551278, Length=527, Percent_Identity=23.1499051233397, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17510401, Length=489, Percent_Identity=22.6993865030675, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=25.9154929577465, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=552, Percent_Identity=25.9057971014493, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323182, Length=563, Percent_Identity=24.866785079929, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355181, Length=472, Percent_Identity=30.9322033898305, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=28.8577154308617, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21356441, Length=466, Percent_Identity=27.4678111587983, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24648255, Length=469, Percent_Identity=28.5714285714286, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24648253, Length=469, Percent_Identity=28.1449893390192, Blast_Score=138, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24648260, Length=481, Percent_Identity=25.5717255717256, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI281365686, Length=377, Percent_Identity=28.1167108753316, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581924, Length=358, Percent_Identity=30.4469273743017, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922652, Length=477, Percent_Identity=25.9958071278826, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356947, Length=468, Percent_Identity=24.1452991452991, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21358303, Length=449, Percent_Identity=24.7216035634744, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648257, Length=354, Percent_Identity=30.5084745762712, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653035, Length=468, Percent_Identity=22.8632478632479, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161076582, Length=476, Percent_Identity=22.6890756302521, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI28573631, Length=539, Percent_Identity=25.0463821892393, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20130353, Length=539, Percent_Identity=25.0463821892393, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20130357, Length=479, Percent_Identity=25.4697286012526, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24656500, Length=473, Percent_Identity=23.8900634249471, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI62472339, Length=520, Percent_Identity=24.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667955, Length=520, Percent_Identity=24.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667959, Length=124, Percent_Identity=37.9032258064516, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50170; Mature: 50039

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRPALVFEEQRYSLERLDALTDGLATALAKHGVRAGERVALMASNRPEFVLAVRAVWR
CCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
LGAAIVLLSPSWRRAEIEHALAVTEPAHAIGDQDALAELLPMWHLDEPIGAGEGPFPTPD
CCCEEEEECCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PHADALLVFSSGTTGLPKAARHTRASFGAAVGHWRDALGLTAADRVQITTPPSHILGLLN
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHH
IVTALETGAWMRLHRRFDLELMLRSIETDRITVEMAVAPIALAIAAHPTLESFDLSSLRY
HHHHHHCCHHHHHHHHCCHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEE
IMWGATPVTASVAETVTRRTGVGWVPAYGASELPVIACNPLAGARLDSVGRAVPGVRLRV
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEE
VSLESGAPVGPGETGEIQARAESLMAGYLPAEATADAFDDGWYRTGDVGNLDADGWLRLT
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHH
DRVKEMVKVRGFQVAPAEVEGVLHAHPAVADCAVFGLPDTADGEVLVAAVVTSTQVDPAE
HHHHHHHHHCCCEECCHHHHHHHHCCCCHHHHEEECCCCCCCCCEEEEEEECCCCCCHHH
LVAWVGERLASYKRPRRIELVEEIPRLPSGKVLRRVLKERYGRTSDD
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDRPALVFEEQRYSLERLDALTDGLATALAKHGVRAGERVALMASNRPEFVLAVRAVWR
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
LGAAIVLLSPSWRRAEIEHALAVTEPAHAIGDQDALAELLPMWHLDEPIGAGEGPFPTPD
CCCEEEEECCCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PHADALLVFSSGTTGLPKAARHTRASFGAAVGHWRDALGLTAADRVQITTPPSHILGLLN
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHH
IVTALETGAWMRLHRRFDLELMLRSIETDRITVEMAVAPIALAIAAHPTLESFDLSSLRY
HHHHHHCCHHHHHHHHCCHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEE
IMWGATPVTASVAETVTRRTGVGWVPAYGASELPVIACNPLAGARLDSVGRAVPGVRLRV
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEE
VSLESGAPVGPGETGEIQARAESLMAGYLPAEATADAFDDGWYRTGDVGNLDADGWLRLT
EEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHH
DRVKEMVKVRGFQVAPAEVEGVLHAHPAVADCAVFGLPDTADGEVLVAAVVTSTQVDPAE
HHHHHHHHHCCCEECCHHHHHHHHCCCCHHHHEEECCCCCCCCCEEEEEEECCCCCCHHH
LVAWVGERLASYKRPRRIELVEEIPRLPSGKVLRRVLKERYGRTSDD
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA