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The map label for this gene is mdeA [H]

Identifier: 158316037

GI number: 158316037

Start: 5074870

End: 5076165

Strand: Reverse

Name: mdeA [H]

Synonym: Franean1_4255

Alternate gene names: 158316037

Gene position: 5076165-5074870 (Counterclockwise)

Preceding gene: 158316041

Following gene: 158316036

Centisome position: 56.51

GC content: 70.68

Gene sequence:

>1296_bases
ATGACCGAGTCGAACTGGTCGTTCGAGACGCAGCAGATCCACGCGGGCGCCCAGCCCGATCCGACGACCGGTGCGCGAGC
CGTCCCGATCTACCAGACGACGAGCTTCGTCTTCCGCGACACCGACCACGCGGCCGCGCTGTTCGCGCTCGGCGAGCCGG
GCAACATCTACACCCGGATCATGAACCCCACCCAGGACGTCTTCGAGCAGCGCGTCGCCGCCCTCGAAGGCGGGGTCGGC
GCGCTCGCCACGGCATCGGGGCAGGCGGCGGAGACGCTGGCGATCCTCAACCTGGCCGAGGCCGGCGACCACATCGTCAG
CTCCGCGAACCTCTACGGCGGTACCTACAACCTGTTCCACTACACGCTGCCCAAGTTCGGCGTCTCGGTGTCGTTCGTCG
ACAACCCGGACGACCTTGACGCCTGGCGGGCCGCGGTACGCCCCGAGACGCGGGCCTTCTACGGCGAGACGATCGGCAAC
CCGCGCGGGGACGTCTTCGACACCGAGGGCGTCGCGGCCGTCGCGCACGAGGCCGGGATACCTCTGATCATCGACAACAC
CCTGGCGACGCCCTACCTGCACCGGCCGTTCGAGCACGGCGCCGACATCGTCGTGCACTCGGCGACCAAGTTCATCGGCG
GCCACGGCACCTCGATCGGCGGGATCATCGTCGACAGCGGCCGGTTCGACTACGGCGCCAGCGGGCGGTTCGCGAACTTC
ACCACCCCCGACCCGAGCTACCACGGCCTGGTCTACTGGGACGCCCTCGGCCACGGCAGCTACATCGCCAAGGCCCGGGT
GCAGCTCCTGCGCGACCTCGGCCCGGCCGTCGCGCCGCTCAACTCCTTCCTGTTCCTGCAGGGCCTGGAGACGCTGTCAC
TGCGGATGGAGCGGCACAGCGCCAACGCCCAGCGGGTCGCCGAGTGGCTGCGGGCGCGCGACGAGGTGAGCTGGGTCGCC
TACCCGAAGCTGGCCGGTTCGCCGTGGTACGAGCGGGCGAGCCGGGTCCTGCCCCGCGGCGCGGGCGCCGTGCTGGCGTT
CGGTATCGAGGGCGGTGCGGCGGCGGGCCGGAAGTTCGTCGAGGGTCTGGAGCTGTTCAGCCACCTCGCCAACATCGGCG
ACGTCCGTTCGCTGGTCATCCACCCGGCGACGACCACCCACTCGCAGCTCACCGACGCCGAGCAGGCGGCGACCGGGGTC
ACCCCCGACCTGATCCGGCTGTCGGTGGGCATCGAGGGCATCGACGACATCCTCGCCGACCTCGACGCAGGCTTCCGGGC
CGCGAAGTCCGGATGA

Upstream 100 bases:

>100_bases
GCGTGGCGCACCCCGTGAGGCCCCCCGTCCACGCCGCCGTCCGAGAACCACCCCGAACCAGGCTCCGCCGTGCCACCACC
GCCAGAGGGGGACTTCCGCC

Downstream 100 bases:

>100_bases
CCGACCAGCGTCAGGACGCCGCACCCGCCAGCCAGCCGGCGCCGCCACCACGGCCACCATCGGGTCCGCGGCCGACGCCG
GCACCGGCACCGCCGTCGCC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTESNWSFETQQIHAGAQPDPTTGARAVPIYQTTSFVFRDTDHAAALFALGEPGNIYTRIMNPTQDVFEQRVAALEGGVG
ALATASGQAAETLAILNLAEAGDHIVSSANLYGGTYNLFHYTLPKFGVSVSFVDNPDDLDAWRAAVRPETRAFYGETIGN
PRGDVFDTEGVAAVAHEAGIPLIIDNTLATPYLHRPFEHGADIVVHSATKFIGGHGTSIGGIIVDSGRFDYGASGRFANF
TTPDPSYHGLVYWDALGHGSYIAKARVQLLRDLGPAVAPLNSFLFLQGLETLSLRMERHSANAQRVAEWLRARDEVSWVA
YPKLAGSPWYERASRVLPRGAGAVLAFGIEGGAAAGRKFVEGLELFSHLANIGDVRSLVIHPATTTHSQLTDAEQAATGV
TPDLIRLSVGIEGIDDILADLDAGFRAAKSG

Sequences:

>Translated_431_residues
MTESNWSFETQQIHAGAQPDPTTGARAVPIYQTTSFVFRDTDHAAALFALGEPGNIYTRIMNPTQDVFEQRVAALEGGVG
ALATASGQAAETLAILNLAEAGDHIVSSANLYGGTYNLFHYTLPKFGVSVSFVDNPDDLDAWRAAVRPETRAFYGETIGN
PRGDVFDTEGVAAVAHEAGIPLIIDNTLATPYLHRPFEHGADIVVHSATKFIGGHGTSIGGIIVDSGRFDYGASGRFANF
TTPDPSYHGLVYWDALGHGSYIAKARVQLLRDLGPAVAPLNSFLFLQGLETLSLRMERHSANAQRVAEWLRARDEVSWVA
YPKLAGSPWYERASRVLPRGAGAVLAFGIEGGAAAGRKFVEGLELFSHLANIGDVRSLVIHPATTTHSQLTDAEQAATGV
TPDLIRLSVGIEGIDDILADLDAGFRAAKSG
>Mature_430_residues
TESNWSFETQQIHAGAQPDPTTGARAVPIYQTTSFVFRDTDHAAALFALGEPGNIYTRIMNPTQDVFEQRVAALEGGVGA
LATASGQAAETLAILNLAEAGDHIVSSANLYGGTYNLFHYTLPKFGVSVSFVDNPDDLDAWRAAVRPETRAFYGETIGNP
RGDVFDTEGVAAVAHEAGIPLIIDNTLATPYLHRPFEHGADIVVHSATKFIGGHGTSIGGIIVDSGRFDYGASGRFANFT
TPDPSYHGLVYWDALGHGSYIAKARVQLLRDLGPAVAPLNSFLFLQGLETLSLRMERHSANAQRVAEWLRARDEVSWVAY
PKLAGSPWYERASRVLPRGAGAVLAFGIEGGAAAGRKFVEGLELFSHLANIGDVRSLVIHPATTTHSQLTDAEQAATGVT
PDLIRLSVGIEGIDDILADLDAGFRAAKSG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=411, Percent_Identity=34.3065693430657, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI299473759, Length=411, Percent_Identity=30.1703163017032, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI25453487, Length=410, Percent_Identity=30, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI1790375, Length=423, Percent_Identity=31.9148936170213, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=27.1662763466042, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=411, Percent_Identity=32.360097323601, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=410, Percent_Identity=32.6829268292683, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=409, Percent_Identity=32.518337408313, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=443, Percent_Identity=51.0158013544018, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319307, Length=436, Percent_Identity=32.7981651376147, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=369, Percent_Identity=28.4552845528455, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=430, Percent_Identity=25.8139534883721, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=31.7647058823529, Blast_Score=202, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46013; Mature: 45882

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESNWSFETQQIHAGAQPDPTTGARAVPIYQTTSFVFRDTDHAAALFALGEPGNIYTRI
CCCCCCCCCHHEEECCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEEEECCCCCEEEEE
MNPTQDVFEQRVAALEGGVGALATASGQAAETLAILNLAEAGDHIVSSANLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEE
YTLPKFGVSVSFVDNPDDLDAWRAAVRPETRAFYGETIGNPRGDVFDTEGVAAVAHEAGI
EECHHHCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCC
PLIIDNTLATPYLHRPFEHGADIVVHSATKFIGGHGTSIGGIIVDSGRFDYGASGRFANF
CEEEECCCCCCHHCCCHHCCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
TTPDPSYHGLVYWDALGHGSYIAKARVQLLRDLGPAVAPLNSFLFLQGLETLSLRMERHS
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ANAQRVAEWLRARDEVSWVAYPKLAGSPWYERASRVLPRGAGAVLAFGIEGGAAAGRKFV
CCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
EGLELFSHLANIGDVRSLVIHPATTTHSQLTDAEQAATGVTPDLIRLSVGIEGIDDILAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHH
LDAGFRAAKSG
HHCCCHHCCCC
>Mature Secondary Structure 
TESNWSFETQQIHAGAQPDPTTGARAVPIYQTTSFVFRDTDHAAALFALGEPGNIYTRI
CCCCCCCCHHEEECCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEEEECCCCCEEEEE
MNPTQDVFEQRVAALEGGVGALATASGQAAETLAILNLAEAGDHIVSSANLYGGTYNLFH
CCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEE
YTLPKFGVSVSFVDNPDDLDAWRAAVRPETRAFYGETIGNPRGDVFDTEGVAAVAHEAGI
EECHHHCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCC
PLIIDNTLATPYLHRPFEHGADIVVHSATKFIGGHGTSIGGIIVDSGRFDYGASGRFANF
CEEEECCCCCCHHCCCHHCCCCEEEECHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
TTPDPSYHGLVYWDALGHGSYIAKARVQLLRDLGPAVAPLNSFLFLQGLETLSLRMERHS
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHC
ANAQRVAEWLRARDEVSWVAYPKLAGSPWYERASRVLPRGAGAVLAFGIEGGAAAGRKFV
CCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
EGLELFSHLANIGDVRSLVIHPATTTHSQLTDAEQAATGVTPDLIRLSVGIEGIDDILAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHH
LDAGFRAAKSG
HHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]