Click here to switch to the map view.

The map label for this gene is polA [C]

Identifier: 158315953

GI number: 158315953

Start: 4958615

End: 4959571

Strand: Reverse

Name: polA [C]

Synonym: Franean1_4171

Alternate gene names: 158315953

Gene position: 4959571-4958615 (Counterclockwise)

Preceding gene: 158315957

Following gene: 158315952

Centisome position: 55.22

GC content: 74.5

Gene sequence:

>957_bases
GTGGATCGGCTGCTGCTCGCGGACACCCCGTCGTTGTATTTCCGGGCTTTTCACGGGGTGCCCCGGTCGGTGCGGGCCCC
CGACGGGATGGCGGTGAACGCGGTGCGGGGTCTGCTGGACGTGCTGGCCCGGCAGATCGTCGAGGTGCGGCCGCGGCGGT
TGGTGTGCTGTTTCGACGCCGACTGGCGGCCGGCGTTCCGGGTGGCGCTGATCGGCTCGTACAAGGCGCATCGGGTCGCC
GGCCCCACCCCCGGCCCCGCGGCCGGGGCTGGTGGGGTGGAGGAGGAGGTGCCGGACGAGTTGGAGGCGCAGCTACCGGT
GATCGACGCGGTGCTCGACGCGTTCGGGATCGCCCGGGTGGAGGCGGCTGGCTTCGAGGCCGACGACGTGATCGGCACGC
TGGCGACCCGGCACAGCGGCCGGCCCGGTGGCGGGCCGGTGGACATCCTGACCGGGGACCGGGATCTGTTCCAGCTGGTG
TGTGACGACACCGGCGTGGTGGTGCGGTACGCGGTGGAACGGTTCGCGGTGGTCGACGAGGCGTCGATCAGCGCCCGCTA
CGGGATCCCTGGCCGGGCCTATGCCGATTTCGCGGTGCTGCGCGGTGATCCCAGCGACGGTCTGCCGGGGGTGGCGGGGA
TCGGCGCGAAGACGGCGGCGGCGCTGCTGGGCCGCTTCGGGTCGCTGCGGGCGATCCTCACCGCGTTGGACGCCGGGGGT
GAGGACGGGTTCCCGGCCGGGGCGCGCCGCCGGCTGACCGCGGCCCGTGACTATGTGGACGCCGCGGTCACCGTGGTCGG
GGTGGTCCGCGATGTGCCGCTGGGCCCGGTCGCCGACGTGCTGCCCGTTCGGCCGGTGGATGAGGCGGCGCTGGCGGCGC
TGGCGGGCCGGTACGGGCTGGGCGGGTCGGTGGACCGGCTGACCATGGCATTGGCCACCCTCGCCGAGGGCCCCTGA

Upstream 100 bases:

>100_bases
GTCGAGATGATCTGTCGGCAGCACATGCCAGTCTGACAACGTGACCAACTTTCACGGTGATTCTTCTGGCGTCGCGCCGC
GGGTGGGGGGTGGTGGGGGT

Downstream 100 bases:

>100_bases
CCTTGTGGGACGGGCATCTCCGGGGGGCGCAGGGGGTGCCGCGTACGGTCACGGGGGCGGGTTCGGGGCGGGTCGGCGCG
GGCCGGCGGCGTGGTCGGAC

Product: 5'-3' exonuclease

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDRLLLADTPSLYFRAFHGVPRSVRAPDGMAVNAVRGLLDVLARQIVEVRPRRLVCCFDADWRPAFRVALIGSYKAHRVA
GPTPGPAAGAGGVEEEVPDELEAQLPVIDAVLDAFGIARVEAAGFEADDVIGTLATRHSGRPGGGPVDILTGDRDLFQLV
CDDTGVVVRYAVERFAVVDEASISARYGIPGRAYADFAVLRGDPSDGLPGVAGIGAKTAAALLGRFGSLRAILTALDAGG
EDGFPAGARRRLTAARDYVDAAVTVVGVVRDVPLGPVADVLPVRPVDEAALAALAGRYGLGGSVDRLTMALATLAEGP

Sequences:

>Translated_318_residues
MDRLLLADTPSLYFRAFHGVPRSVRAPDGMAVNAVRGLLDVLARQIVEVRPRRLVCCFDADWRPAFRVALIGSYKAHRVA
GPTPGPAAGAGGVEEEVPDELEAQLPVIDAVLDAFGIARVEAAGFEADDVIGTLATRHSGRPGGGPVDILTGDRDLFQLV
CDDTGVVVRYAVERFAVVDEASISARYGIPGRAYADFAVLRGDPSDGLPGVAGIGAKTAAALLGRFGSLRAILTALDAGG
EDGFPAGARRRLTAARDYVDAAVTVVGVVRDVPLGPVADVLPVRPVDEAALAALAGRYGLGGSVDRLTMALATLAEGP
>Mature_318_residues
MDRLLLADTPSLYFRAFHGVPRSVRAPDGMAVNAVRGLLDVLARQIVEVRPRRLVCCFDADWRPAFRVALIGSYKAHRVA
GPTPGPAAGAGGVEEEVPDELEAQLPVIDAVLDAFGIARVEAAGFEADDVIGTLATRHSGRPGGGPVDILTGDRDLFQLV
CDDTGVVVRYAVERFAVVDEASISARYGIPGRAYADFAVLRGDPSDGLPGVAGIGAKTAAALLGRFGSLRAILTALDAGG
EDGFPAGARRRLTAARDYVDAAVTVVGVVRDVPLGPVADVLPVRPVDEAALAALAGRYGLGGSVDRLTMALATLAEGP

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=226, Percent_Identity=34.5132743362832, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI226510970, Length=256, Percent_Identity=28.90625, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32998; Mature: 32998

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLLLADTPSLYFRAFHGVPRSVRAPDGMAVNAVRGLLDVLARQIVEVRPRRLVCCFDA
CCCEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
DWRPAFRVALIGSYKAHRVAGPTPGPAAGAGGVEEEVPDELEAQLPVIDAVLDAFGIARV
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHCHHHHHHHHHHHHHHH
EAAGFEADDVIGTLATRHSGRPGGGPVDILTGDRDLFQLVCDDTGVVVRYAVERFAVVDE
HHCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHCC
ASISARYGIPGRAYADFAVLRGDPSDGLPGVAGIGAKTAAALLGRFGSLRAILTALDAGG
CCCCEECCCCCCHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EDGFPAGARRRLTAARDYVDAAVTVVGVVRDVPLGPVADVLPVRPVDEAALAALAGRYGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCCCC
GGSVDRLTMALATLAEGP
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDRLLLADTPSLYFRAFHGVPRSVRAPDGMAVNAVRGLLDVLARQIVEVRPRRLVCCFDA
CCCEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
DWRPAFRVALIGSYKAHRVAGPTPGPAAGAGGVEEEVPDELEAQLPVIDAVLDAFGIARV
CCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHCHHHHHHHHHHHHHHH
EAAGFEADDVIGTLATRHSGRPGGGPVDILTGDRDLFQLVCDDTGVVVRYAVERFAVVDE
HHCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHHHHHHCC
ASISARYGIPGRAYADFAVLRGDPSDGLPGVAGIGAKTAAALLGRFGSLRAILTALDAGG
CCCCEECCCCCCHHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
EDGFPAGARRRLTAARDYVDAAVTVVGVVRDVPLGPVADVLPVRPVDEAALAALAGRYGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCCCC
GGSVDRLTMALATLAEGP
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]