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The map label for this gene is ynfL [H]

Identifier: 158315751

GI number: 158315751

Start: 4745045

End: 4745992

Strand: Reverse

Name: ynfL [H]

Synonym: Franean1_3963

Alternate gene names: 158315751

Gene position: 4745992-4745045 (Counterclockwise)

Preceding gene: 158315752

Following gene: 158315750

Centisome position: 52.84

GC content: 75.11

Gene sequence:

>948_bases
ATGGAACTGAGATATCTCGCGTCGTTCCTCGCCATCGCCGAGGAGCTTCACTTCGGCCGCGCGGCGGCCCGCCTGCACCT
GGCCCAGCCCTCGCTGAGCCAGCAGCTTCAGCGGCTGGAACGCGACATCGGCGTCGAGCTGGTCTCGCGCAGCTCGCACG
AGGTCAAGCTGACCGCCGCCGGCCGCGCCTTCGAGACGGAGGCCCGCCGGCTGCTCGACGCCACCCGGCGGGCCGTCACC
GTCGCCCGCGAGGCGGCCTCGGGGCGGACGGGGATGATCAGCATCGGCTTCAACTACCCCGCCGGCCAGCGGGTGCTCCA
GCCGACGCTGCTGCGCCTGGCGGCCGAGTACCCGGGCGTGTCGACGACGCTGTGGGAGGCCCGCAGCGGGCCGCAGCTGT
CCGCGCTGGCCGAGGGACGGCTTGACGTCGCGCTGGTCTTCGCCGGGCCGCCCTCGGCCCAGCTGCGCTCCCAGCGGGTG
CTCACCGTGCCGCTGGTCGCGGTGGTCAGCCGGCGCCATCCCTGGGCGGCCCGGTCCGAGGTTCCCTTCCGGGAGCTGGC
CGACCAGCCGTGTGTCCTGTTCCGGCGGGAGCAGAGCCCGGCGATGCACGACGCGATCCTCGCCGCCGCCGACCGCTGTG
GCATCGCGCTGACCGTCTCCGACGAGGTGGACGACTCCGGCGCGACCGGGATCGTGGTGACCACCAAGCCGGTGGTCGGG
TTCGCCTCGGCCGCGCGCGGCGCGCACCCACCCGGTCACGGGCTCGCCGCGGTGCGCCTGGTCGACCCGACCCCGACAGT
CGGCATGTACGCCGTGTGGCGCCCGGACCCACAGCCCGTGGTCGGCGCCTTCCTGGACTGCCTCGAGGCCGCCGGCCCGT
TCACCGAGCCCGGCCCGCCCGCGGCGGTGGCACCGGCACCGGCACCGGCACCGGTCAAGGAGAGCTGA

Upstream 100 bases:

>100_bases
ACGACGACATGCCACGTTCCCGTTCCGCCGTCCACACGTGCGCGTTACCGGCGGTTAGCGCCCACCGGTAACGTGAGCGG
CGCCCAGCGGGAGGATGGCG

Downstream 100 bases:

>100_bases
CCCCGGGTCCCCGTCCGGCGGGCGGTGCGGTGCGGTGCGGTGCCCGGCGATTGGCATCCCGAATTGCTCGTTCGCCATTC
ATGCGTTGCCGAGAACCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MELRYLASFLAIAEELHFGRAAARLHLAQPSLSQQLQRLERDIGVELVSRSSHEVKLTAAGRAFETEARRLLDATRRAVT
VAREAASGRTGMISIGFNYPAGQRVLQPTLLRLAAEYPGVSTTLWEARSGPQLSALAEGRLDVALVFAGPPSAQLRSQRV
LTVPLVAVVSRRHPWAARSEVPFRELADQPCVLFRREQSPAMHDAILAAADRCGIALTVSDEVDDSGATGIVVTTKPVVG
FASAARGAHPPGHGLAAVRLVDPTPTVGMYAVWRPDPQPVVGAFLDCLEAAGPFTEPGPPAAVAPAPAPAPVKES

Sequences:

>Translated_315_residues
MELRYLASFLAIAEELHFGRAAARLHLAQPSLSQQLQRLERDIGVELVSRSSHEVKLTAAGRAFETEARRLLDATRRAVT
VAREAASGRTGMISIGFNYPAGQRVLQPTLLRLAAEYPGVSTTLWEARSGPQLSALAEGRLDVALVFAGPPSAQLRSQRV
LTVPLVAVVSRRHPWAARSEVPFRELADQPCVLFRREQSPAMHDAILAAADRCGIALTVSDEVDDSGATGIVVTTKPVVG
FASAARGAHPPGHGLAAVRLVDPTPTVGMYAVWRPDPQPVVGAFLDCLEAAGPFTEPGPPAAVAPAPAPAPVKES
>Mature_315_residues
MELRYLASFLAIAEELHFGRAAARLHLAQPSLSQQLQRLERDIGVELVSRSSHEVKLTAAGRAFETEARRLLDATRRAVT
VAREAASGRTGMISIGFNYPAGQRVLQPTLLRLAAEYPGVSTTLWEARSGPQLSALAEGRLDVALVFAGPPSAQLRSQRV
LTVPLVAVVSRRHPWAARSEVPFRELADQPCVLFRREQSPAMHDAILAAADRCGIALTVSDEVDDSGATGIVVTTKPVVG
FASAARGAHPPGHGLAAVRLVDPTPTVGMYAVWRPDPQPVVGAFLDCLEAAGPFTEPGPPAAVAPAPAPAPVKES

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=35.0806451612903, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790399, Length=198, Percent_Identity=31.8181818181818, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI2367136, Length=192, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI157672245, Length=197, Percent_Identity=33.502538071066, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788887, Length=224, Percent_Identity=31.25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788297, Length=175, Percent_Identity=30.8571428571429, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790208, Length=151, Percent_Identity=33.112582781457, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693105, Length=186, Percent_Identity=23.6559139784946, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789639, Length=171, Percent_Identity=28.0701754385965, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081904, Length=218, Percent_Identity=26.605504587156, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI48994962, Length=282, Percent_Identity=26.9503546099291, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=221, Percent_Identity=23.9819004524887, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=23.8636363636364, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33426; Mature: 33426

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRYLASFLAIAEELHFGRAAARLHLAQPSLSQQLQRLERDIGVELVSRSSHEVKLTAA
CCHHHHHHHHHHHHHHHHCHHHHEEEECCCCHHHHHHHHHHHHCHHEECCCCCEEEEEEC
GRAFETEARRLLDATRRAVTVAREAASGRTGMISIGFNYPAGQRVLQPTLLRLAAEYPGV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCC
STTLWEARSGPQLSALAEGRLDVALVFAGPPSAQLRSQRVLTVPLVAVVSRRHPWAARSE
CEEEECCCCCCCHHHHHCCCEEEEEEEECCCCHHHHCCCEEEHHHHHHHHCCCCCCCCCC
VPFRELADQPCVLFRREQSPAMHDAILAAADRCGIALTVSDEVDDSGATGIVVTTKPVVG
CCHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHH
FASAARGAHPPGHGLAAVRLVDPTPTVGMYAVWRPDPQPVVGAFLDCLEAAGPFTEPGPP
HHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCC
AAVAPAPAPAPVKES
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MELRYLASFLAIAEELHFGRAAARLHLAQPSLSQQLQRLERDIGVELVSRSSHEVKLTAA
CCHHHHHHHHHHHHHHHHCHHHHEEEECCCCHHHHHHHHHHHHCHHEECCCCCEEEEEEC
GRAFETEARRLLDATRRAVTVAREAASGRTGMISIGFNYPAGQRVLQPTLLRLAAEYPGV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCC
STTLWEARSGPQLSALAEGRLDVALVFAGPPSAQLRSQRVLTVPLVAVVSRRHPWAARSE
CEEEECCCCCCCHHHHHCCCEEEEEEEECCCCHHHHCCCEEEHHHHHHHHCCCCCCCCCC
VPFRELADQPCVLFRREQSPAMHDAILAAADRCGIALTVSDEVDDSGATGIVVTTKPVVG
CCHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCHH
FASAARGAHPPGHGLAAVRLVDPTPTVGMYAVWRPDPQPVVGAFLDCLEAAGPFTEPGPP
HHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCC
AAVAPAPAPAPVKES
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]