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The map label for this gene is gsiD [H]

Identifier: 158315709

GI number: 158315709

Start: 4688805

End: 4689704

Strand: Reverse

Name: gsiD [H]

Synonym: Franean1_3921

Alternate gene names: 158315709

Gene position: 4689704-4688805 (Counterclockwise)

Preceding gene: 158315710

Following gene: 158315708

Centisome position: 52.21

GC content: 69.44

Gene sequence:

>900_bases
ATGACCACCCCCGACGTGGTGGACCGCGAGGTCCACTTACCGCCGGCCCCGGCGCTGGACACGCCGATCGTCCCGGCGGT
GCGAAAGTCCCGCTCGATCCTGGTGTACCTGGCCTACGCCTGGCTGATCGCGGTGATCGCGCTCGCCGCGCTGGCGGACG
TGCTGCCGCTGGCGTCGTACTCCATTCCCATCGGGAAGCCGCGCCAGGGACCGGATTTCAGCTCGTTCGACCTGTGGCTG
GGCACCGACCAGCAGGGCCGGTCGATCCTGTCGCGATGCGTGTACGGTGCGCGCGTCTCGCTCCTGGTCGGAACCGTGGC
GGGCCTCATCGGGGCCGTCATCGGAACCCTGCTGGGCATGCTCGCCGGCTACCTCGGCAAGGCCGTCGACTGGATCATCC
GGCTGATCACCGACGCGATGCTGGCGTTCCCGCCACTGATCCTGCTCCTGGCGCTGTCGTCCATCCTCACGCCGAGCGTG
CGGACGCTGCTGGTCGGCCTGACGCTGCTGATCATCCCGACGTTCGTCCGGCTCGCGCTGGCGAACACCCTCGCCTGGTC
GTCCCGCGAGTTCGTCACCGCCGCCCGCAACATGGGCGCGGGGCACGTGCGGATCCTGGTGAAGGAGATCCTGCCGAACC
TGCTGCCACCACTGGGCGCGTTCCTGCCGGTCGTGATGGCCGCGCTGATCGTGGCCGAAGGGTCGCTGAGCTTCCTGGGG
ATGGGCATCCCGCCGCCCCAGCCCAGCTGGGGCGGCATGATCTCCGACGGCAAGGAGGCCATCGCCGACTCCCCGCACAT
GGTGCTGGTGCCGGCGATCGTCATCTTCTTCACCGTCTTCGCGCTGAACCAGGCGGGCGACCACCTGCGCAGCCGCTTCG
ACCGCACGATGCGCGACTGA

Upstream 100 bases:

>100_bases
GCATCGTCGTGTTCATCGCCGTGGTCTACGTGGTCATCAACATGCTCGTGGATCTCAGTTACGGCTGGCTCGACCCGCGC
GTCAGGAAGGCTGCCAACGC

Downstream 100 bases:

>100_bases
CGACCCGCCTGAGCAGGACGCGCGACCGAACGCACCCCACTTGGCGTGCGCGACTGGCTCTTCCCAGCGCGCACCGCCTG
GGCCGTGAACAAACGGCGCT

Product: binding-protein-dependent transport systems inner membrane component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTTPDVVDREVHLPPAPALDTPIVPAVRKSRSILVYLAYAWLIAVIALAALADVLPLASYSIPIGKPRQGPDFSSFDLWL
GTDQQGRSILSRCVYGARVSLLVGTVAGLIGAVIGTLLGMLAGYLGKAVDWIIRLITDAMLAFPPLILLLALSSILTPSV
RTLLVGLTLLIIPTFVRLALANTLAWSSREFVTAARNMGAGHVRILVKEILPNLLPPLGAFLPVVMAALIVAEGSLSFLG
MGIPPPQPSWGGMISDGKEAIADSPHMVLVPAIVIFFTVFALNQAGDHLRSRFDRTMRD

Sequences:

>Translated_299_residues
MTTPDVVDREVHLPPAPALDTPIVPAVRKSRSILVYLAYAWLIAVIALAALADVLPLASYSIPIGKPRQGPDFSSFDLWL
GTDQQGRSILSRCVYGARVSLLVGTVAGLIGAVIGTLLGMLAGYLGKAVDWIIRLITDAMLAFPPLILLLALSSILTPSV
RTLLVGLTLLIIPTFVRLALANTLAWSSREFVTAARNMGAGHVRILVKEILPNLLPPLGAFLPVVMAALIVAEGSLSFLG
MGIPPPQPSWGGMISDGKEAIADSPHMVLVPAIVIFFTVFALNQAGDHLRSRFDRTMRD
>Mature_298_residues
TTPDVVDREVHLPPAPALDTPIVPAVRKSRSILVYLAYAWLIAVIALAALADVLPLASYSIPIGKPRQGPDFSSFDLWLG
TDQQGRSILSRCVYGARVSLLVGTVAGLIGAVIGTLLGMLAGYLGKAVDWIIRLITDAMLAFPPLILLLALSSILTPSVR
TLLVGLTLLIIPTFVRLALANTLAWSSREFVTAARNMGAGHVRILVKEILPNLLPPLGAFLPVVMAALIVAEGSLSFLGM
GIPPPQPSWGGMISDGKEAIADSPHMVLVPAIVIFFTVFALNQAGDHLRSRFDRTMRD

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=227, Percent_Identity=38.3259911894273, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787760, Length=216, Percent_Identity=35.1851851851852, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789964, Length=220, Percent_Identity=41.3636363636364, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789889, Length=216, Percent_Identity=31.4814814814815, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787498, Length=224, Percent_Identity=33.4821428571429, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788505, Length=217, Percent_Identity=32.258064516129, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787549, Length=259, Percent_Identity=28.1853281853282, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31895; Mature: 31764

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPDVVDREVHLPPAPALDTPIVPAVRKSRSILVYLAYAWLIAVIALAALADVLPLASY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SIPIGKPRQGPDFSSFDLWLGTDQQGRSILSRCVYGARVSLLVGTVAGLIGAVIGTLLGM
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGYLGKAVDWIIRLITDAMLAFPPLILLLALSSILTPSVRTLLVGLTLLIIPTFVRLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ANTLAWSSREFVTAARNMGAGHVRILVKEILPNLLPPLGAFLPVVMAALIVAEGSLSFLG
HHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHEE
MGIPPPQPSWGGMISDGKEAIADSPHMVLVPAIVIFFTVFALNQAGDHLRSRFDRTMRD
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTPDVVDREVHLPPAPALDTPIVPAVRKSRSILVYLAYAWLIAVIALAALADVLPLASY
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SIPIGKPRQGPDFSSFDLWLGTDQQGRSILSRCVYGARVSLLVGTVAGLIGAVIGTLLGM
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGYLGKAVDWIIRLITDAMLAFPPLILLLALSSILTPSVRTLLVGLTLLIIPTFVRLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ANTLAWSSREFVTAARNMGAGHVRILVKEILPNLLPPLGAFLPVVMAALIVAEGSLSFLG
HHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHEE
MGIPPPQPSWGGMISDGKEAIADSPHMVLVPAIVIFFTVFALNQAGDHLRSRFDRTMRD
CCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]