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The map label for this gene is gsiB [H]

Identifier: 158315708

GI number: 158315708

Start: 4687062

End: 4688633

Strand: Reverse

Name: gsiB [H]

Synonym: Franean1_3920

Alternate gene names: 158315708

Gene position: 4688633-4687062 (Counterclockwise)

Preceding gene: 158315709

Following gene: 158315707

Centisome position: 52.2

GC content: 67.3

Gene sequence:

>1572_bases
ATGTTCCGTAAGTCCCGGTTAGTCGCCACCGCTGTCGTGTGCGGCGCGGCACTCGCCCTGGGGGCCTGTGGTGGTGGAGG
CGGTGACGACGCCACCTCGTCGACGAACGGCGCCGCGGGCCAACCGGTCGCCGGCGGCGAGGGCCGGATCCTGCTGCTGG
GCGACCCGCGCAGCCTGGACCCGGCGACCCTCAGCAACCAGGCCGCGATCACCGCCCCGGTCGGCAACGCGCTGTACGGC
ACGCTGATGATCACCGACCAGGCCGGCAAGGTCAAGTACACGATGGCCGAGTCGTTCGACACGACCGACACCGGCAAGAC
CTTCACCCTCAAGCTGAAGCACGGCCTGGTGTTCTCCGACGGCAAGCCGCTCAACGCGGAGGCCGTCAAGTTCAACTGGG
ACCGCATCAAGGACCCGACCGTGGGCTCCTCCTACGTCGTGGACGCGCGGATGATCGAGTCGACCGAGGTGGTCGACGAC
GTGACACTGAAGGTCACGATGGTCAACCCCGTGCCGGCGTACGCCCAGGCCGTCCTGAACTCGTCACTGAACTGGATCGC
CTCGCCCGACGCTCTGAAGGCCGGACGGGACTCCTTCGACAAGAACCCGATCGGCGCCGGGCCGTTCACCCTGGCGAGCT
GGACCCGCCAGGCGGACATCAAGTTCGTCAAGAACCCCAAGTACTGGGACGCGCCCAAGCCCTACCTGGACCGCCTCACC
ATGCGCTCGGCGACCGACGCCACCCAGCGCCTCAACACGGTGATCAGCGGTGGCGCCGACGTCGCGATCGACACGAACAC
GGTCAACATCGACAAGGCTGAGACGTCCGATCTCAACGCGGTCGTGACCACCCTCAACGGCGGCAACTTCATGGCGTTCA
ACTCGCGCCGGGCGCCGTTCGACGACATCCGTGCCCGCCAGGCGGTGTCGGCGGCGATCGACCTCGAGGCGCTCAACCTC
GCCGCCTACAACGGCACCGCTCCCCTGCCCGACACGCTGTTCGACAAGAGCTCACCTCTCTTCTCGGACACGCCGCTACA
CAAGACGGACAAGGCTCTCGCCCAGAAGCTCCTCGACGAGCTCGCCGCCGACGGCAAGCCGGTGAAGTTCACCTTCTCCA
GCTTCCCGTCCTCGGAGAACCGGGCGATCGCGGAGAACATCCAGGCCCAGCTGAGCGCCTTCAAGAACATCACGGTCTCC
GTCAAGATCGTCGACCTCGGCCAGGTCGCGGCGCTGCGCACGACCTTCGACTTCGACCTGCTCGTCTCGTCGGCGTCGTT
CCAGGACCCGGAGCCGCGGCTGTGGCAGGCGTTCAGCCAGGACTCCGTGGCGAACCTGTCCGGTGTCAAGGACAAGGAGC
TCTCGGACGCGCTGCTCGCGGGTCGCACCGCGACGACGGAGGCGGACCGCAAGGCCGCCTACGAGACGGTGCAGGAGCGG
CTGGTCGCGCTCAGCCCGGTCGTGTTCTACCAGCGGTCGACGAACGCGGCGATCGGCACCGCCAAGGTCGGCGGGATCGT
CCAGTACGGCAGCGGCTCGCTGCTGGTCGAGGAACTCTGGATCAAGAAGTAG

Upstream 100 bases:

>100_bases
CACCGCCTGGGCCGTGAACAAACGGCGCTGCCCCCACCCGCCGTTTGCACGGCTTCCCGTTCCACACTCTCCGTTTCTAG
ATGCTGACGAAAGGTCAACG

Downstream 100 bases:

>100_bases
TCGTAGATCCGGAGGATGGATGAGAGGTCCCTGCGCCCATGAGTACTGATCCTGTCCTGCAGGTGCGCAATCTGCGCGCC
TACATCGGCACCCCGCGTGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MFRKSRLVATAVVCGAALALGACGGGGGDDATSSTNGAAGQPVAGGEGRILLLGDPRSLDPATLSNQAAITAPVGNALYG
TLMITDQAGKVKYTMAESFDTTDTGKTFTLKLKHGLVFSDGKPLNAEAVKFNWDRIKDPTVGSSYVVDARMIESTEVVDD
VTLKVTMVNPVPAYAQAVLNSSLNWIASPDALKAGRDSFDKNPIGAGPFTLASWTRQADIKFVKNPKYWDAPKPYLDRLT
MRSATDATQRLNTVISGGADVAIDTNTVNIDKAETSDLNAVVTTLNGGNFMAFNSRRAPFDDIRARQAVSAAIDLEALNL
AAYNGTAPLPDTLFDKSSPLFSDTPLHKTDKALAQKLLDELAADGKPVKFTFSSFPSSENRAIAENIQAQLSAFKNITVS
VKIVDLGQVAALRTTFDFDLLVSSASFQDPEPRLWQAFSQDSVANLSGVKDKELSDALLAGRTATTEADRKAAYETVQER
LVALSPVVFYQRSTNAAIGTAKVGGIVQYGSGSLLVEELWIKK

Sequences:

>Translated_523_residues
MFRKSRLVATAVVCGAALALGACGGGGGDDATSSTNGAAGQPVAGGEGRILLLGDPRSLDPATLSNQAAITAPVGNALYG
TLMITDQAGKVKYTMAESFDTTDTGKTFTLKLKHGLVFSDGKPLNAEAVKFNWDRIKDPTVGSSYVVDARMIESTEVVDD
VTLKVTMVNPVPAYAQAVLNSSLNWIASPDALKAGRDSFDKNPIGAGPFTLASWTRQADIKFVKNPKYWDAPKPYLDRLT
MRSATDATQRLNTVISGGADVAIDTNTVNIDKAETSDLNAVVTTLNGGNFMAFNSRRAPFDDIRARQAVSAAIDLEALNL
AAYNGTAPLPDTLFDKSSPLFSDTPLHKTDKALAQKLLDELAADGKPVKFTFSSFPSSENRAIAENIQAQLSAFKNITVS
VKIVDLGQVAALRTTFDFDLLVSSASFQDPEPRLWQAFSQDSVANLSGVKDKELSDALLAGRTATTEADRKAAYETVQER
LVALSPVVFYQRSTNAAIGTAKVGGIVQYGSGSLLVEELWIKK
>Mature_523_residues
MFRKSRLVATAVVCGAALALGACGGGGGDDATSSTNGAAGQPVAGGEGRILLLGDPRSLDPATLSNQAAITAPVGNALYG
TLMITDQAGKVKYTMAESFDTTDTGKTFTLKLKHGLVFSDGKPLNAEAVKFNWDRIKDPTVGSSYVVDARMIESTEVVDD
VTLKVTMVNPVPAYAQAVLNSSLNWIASPDALKAGRDSFDKNPIGAGPFTLASWTRQADIKFVKNPKYWDAPKPYLDRLT
MRSATDATQRLNTVISGGADVAIDTNTVNIDKAETSDLNAVVTTLNGGNFMAFNSRRAPFDDIRARQAVSAAIDLEALNL
AAYNGTAPLPDTLFDKSSPLFSDTPLHKTDKALAQKLLDELAADGKPVKFTFSSFPSSENRAIAENIQAQLSAFKNITVS
VKIVDLGQVAALRTTFDFDLLVSSASFQDPEPRLWQAFSQDSVANLSGVKDKELSDALLAGRTATTEADRKAAYETVQER
LVALSPVVFYQRSTNAAIGTAKVGGIVQYGSGSLLVEELWIKK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=361, Percent_Identity=29.0858725761773, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1789397, Length=472, Percent_Identity=26.4830508474576, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=495, Percent_Identity=26.0606060606061, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789966, Length=346, Percent_Identity=26.878612716763, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787762, Length=489, Percent_Identity=25.5623721881391, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789887, Length=438, Percent_Identity=25.3424657534247, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081878, Length=478, Percent_Identity=22.8033472803347, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787551, Length=269, Percent_Identity=21.5613382899628, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55606; Mature: 55606

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKSRLVATAVVCGAALALGACGGGGGDDATSSTNGAAGQPVAGGEGRILLLGDPRSLD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
PATLSNQAAITAPVGNALYGTLMITDQAGKVKYTMAESFDTTDTGKTFTLKLKHGLVFSD
CCCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCEEECC
GKPLNAEAVKFNWDRIKDPTVGSSYVVDARMIESTEVVDDVTLKVTMVNPVPAYAQAVLN
CCCCCCCEEEEEHHHCCCCCCCCCEEEEEEHHCCHHHCCCEEEEEEEECCCHHHHHHHHH
SSLNWIASPDALKAGRDSFDKNPIGAGPFTLASWTRQADIKFVKNPKYWDAPKPYLDRLT
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHH
MRSATDATQRLNTVISGGADVAIDTNTVNIDKAETSDLNAVVTTLNGGNFMAFNSRRAPF
HHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCEEEEEEECCCEEEEECCCCCCH
DDIRARQAVSAAIDLEALNLAAYNGTAPLPDTLFDKSSPLFSDTPLHKTDKALAQKLLDE
HHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
LAADGKPVKFTFSSFPSSENRAIAENIQAQLSAFKNITVSVKIVDLGQVAALRTTFDFDL
HHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEEECCEEE
LVSSASFQDPEPRLWQAFSQDSVANLSGVKDKELSDALLAGRTATTEADRKAAYETVQER
EEECCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LVALSPVVFYQRSTNAAIGTAKVGGIVQYGSGSLLVEELWIKK
HHHHCCEEEEECCCCCEEEEHHCCCEEEECCCCEEEHHHHCCC
>Mature Secondary Structure
MFRKSRLVATAVVCGAALALGACGGGGGDDATSSTNGAAGQPVAGGEGRILLLGDPRSLD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
PATLSNQAAITAPVGNALYGTLMITDQAGKVKYTMAESFDTTDTGKTFTLKLKHGLVFSD
CCCCCCCCEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEECCEEECC
GKPLNAEAVKFNWDRIKDPTVGSSYVVDARMIESTEVVDDVTLKVTMVNPVPAYAQAVLN
CCCCCCCEEEEEHHHCCCCCCCCCEEEEEEHHCCHHHCCCEEEEEEEECCCHHHHHHHHH
SSLNWIASPDALKAGRDSFDKNPIGAGPFTLASWTRQADIKFVKNPKYWDAPKPYLDRLT
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEECCCCCCCCCCHHHHHHH
MRSATDATQRLNTVISGGADVAIDTNTVNIDKAETSDLNAVVTTLNGGNFMAFNSRRAPF
HHHHHHHHHHHHHHHCCCCEEEEECCEEECCCCCCCCCEEEEEEECCCEEEEECCCCCCH
DDIRARQAVSAAIDLEALNLAAYNGTAPLPDTLFDKSSPLFSDTPLHKTDKALAQKLLDE
HHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
LAADGKPVKFTFSSFPSSENRAIAENIQAQLSAFKNITVSVKIVDLGQVAALRTTFDFDL
HHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEEECCEEE
LVSSASFQDPEPRLWQAFSQDSVANLSGVKDKELSDALLAGRTATTEADRKAAYETVQER
EEECCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LVALSPVVFYQRSTNAAIGTAKVGGIVQYGSGSLLVEELWIKK
HHHHCCEEEEECCCCCEEEEHHCCCEEEECCCCEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA