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The map label for this gene is acoA [H]

Identifier: 158315117

GI number: 158315117

Start: 3928344

End: 3929342

Strand: Direct

Name: acoA [H]

Synonym: Franean1_3316

Alternate gene names: 158315117

Gene position: 3928344-3929342 (Clockwise)

Preceding gene: 158315116

Following gene: 158315118

Centisome position: 43.74

GC content: 71.57

Gene sequence:

>999_bases
GTGACGACGGAGCAGGCGGCGGAGCCGGCGCTCGATGTGCGGCGTCGGCTGTACGAGCTGATGACGCTCATGAAGGCGGC
CGACGATCGGCTGAGCAAGGGGATCGCCTCGGGCGAGCTGCAGTGCGTGTACTGGCCCTCACGTGGTCAGGAGGCGATCG
CCGCGGCGGTGGGCGTGGTCCTCCGCCTGGACGATCAGCTGGTCACGACGTACCGCGGACTGCACGACCTGATCGGCAAG
GGCGTGCCGCTGGTCGAGATCTACGGCGAGATGCTGGGCCGCCAGGTCGGCTCCGGCCGCGGCAAGGGCGGGACGATGCA
CATCGCGCATCCCGACTCCGGCCTCATGATGTCGACGGGGATCGTGGGCGCGGGGCCCCCGGTCGCGGTCGGGCTGGCCA
TGGCCGCACGGCGCAAGGGCCTCGACCGGGTGACGGTCGCCAGCTTCGGTGACGGGGCCACGAACACCGGCTCCTTCCAC
GAGGCGGCGAACATGGCGGCGCTGTGGGACCTGCCGCTGGTGCTGGTCTGCCAGAACAACCAGTACGCCGAGATGACGCC
GACCGGGCACACGATGAAGATCGCCCAGGTCGCGGACCGGGCGGCCGGCTACGGGATGCCGGGCGTCCGCGTCGACGGCA
ACGACCCCCTCGCCGTCGTCGCCGCGCTGGAGGAGGCCGTCGAGCGGGCGAGAGCCGGCGCCGGCCCAACGCTCCTGGAA
TGCGTGACGTTCCGGTTCCGCGGCCACTACTTCGGCGATCCGATGAAGTACATCCCGACGGAGCAGATGGACGCCGCGAT
CGCGGCTGATCCCCTCCCGCGTTTCCGGTCACGTCTGTTGGACGACGGGATCTGCGGTGCGCGGGAGCTCGACGAGATCG
AGACGGCCGCCGTCTCGGCGGTTGACGAGGCGCTGGCCGCCGTCCTGGCCGCGCCCGCCGCGGCACTCGACGAGCTGGAC
CGGGACGTGTACGCCGATCCGCGCAACTGCCCCGCGTAG

Upstream 100 bases:

>100_bases
GTCCTGACCAACCAGCTGCTCGCCGCCGGGAGCGAGGAATGACCGGAGGGGTCCGCCCCGGCCGCCGCCAGCCGGAGACC
GGGTTAGACGAGGAGGGGCT

Downstream 100 bases:

>100_bases
CGCAACTGCCCCGCGTAGCGCGACTGCCCCCGTAACGCGCGCTTAGAACGTGCATTCCGTATCGAACGTGGCCTGGCAAT
GAGGTGGACGTGGACGAACG

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTTEQAAEPALDVRRRLYELMTLMKAADDRLSKGIASGELQCVYWPSRGQEAIAAAVGVVLRLDDQLVTTYRGLHDLIGK
GVPLVEIYGEMLGRQVGSGRGKGGTMHIAHPDSGLMMSTGIVGAGPPVAVGLAMAARRKGLDRVTVASFGDGATNTGSFH
EAANMAALWDLPLVLVCQNNQYAEMTPTGHTMKIAQVADRAAGYGMPGVRVDGNDPLAVVAALEEAVERARAGAGPTLLE
CVTFRFRGHYFGDPMKYIPTEQMDAAIAADPLPRFRSRLLDDGICGARELDEIETAAVSAVDEALAAVLAAPAAALDELD
RDVYADPRNCPA

Sequences:

>Translated_332_residues
MTTEQAAEPALDVRRRLYELMTLMKAADDRLSKGIASGELQCVYWPSRGQEAIAAAVGVVLRLDDQLVTTYRGLHDLIGK
GVPLVEIYGEMLGRQVGSGRGKGGTMHIAHPDSGLMMSTGIVGAGPPVAVGLAMAARRKGLDRVTVASFGDGATNTGSFH
EAANMAALWDLPLVLVCQNNQYAEMTPTGHTMKIAQVADRAAGYGMPGVRVDGNDPLAVVAALEEAVERARAGAGPTLLE
CVTFRFRGHYFGDPMKYIPTEQMDAAIAADPLPRFRSRLLDDGICGARELDEIETAAVSAVDEALAAVLAAPAAALDELD
RDVYADPRNCPA
>Mature_331_residues
TTEQAAEPALDVRRRLYELMTLMKAADDRLSKGIASGELQCVYWPSRGQEAIAAAVGVVLRLDDQLVTTYRGLHDLIGKG
VPLVEIYGEMLGRQVGSGRGKGGTMHIAHPDSGLMMSTGIVGAGPPVAVGLAMAARRKGLDRVTVASFGDGATNTGSFHE
AANMAALWDLPLVLVCQNNQYAEMTPTGHTMKIAQVADRAAGYGMPGVRVDGNDPLAVVAALEEAVERARAGAGPTLLEC
VTFRFRGHYFGDPMKYIPTEQMDAAIAADPLPRFRSRLLDDGICGARELDEIETAAVSAVDEALAAVLAAPAAALDELDR
DVYADPRNCPA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=280, Percent_Identity=33.9285714285714, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI291084742, Length=282, Percent_Identity=34.7517730496454, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4505685, Length=282, Percent_Identity=34.7517730496454, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI291084744, Length=281, Percent_Identity=34.5195729537367, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI11386135, Length=314, Percent_Identity=29.6178343949045, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI258645172, Length=313, Percent_Identity=29.3929712460064, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI291084757, Length=282, Percent_Identity=30.4964539007092, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17536047, Length=288, Percent_Identity=34.7222222222222, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32564172, Length=288, Percent_Identity=34.7222222222222, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI86563355, Length=327, Percent_Identity=27.82874617737, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI86563357, Length=327, Percent_Identity=27.82874617737, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321026, Length=289, Percent_Identity=35.2941176470588, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24639748, Length=327, Percent_Identity=33.6391437308869, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24639746, Length=280, Percent_Identity=33.9285714285714, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24639740, Length=280, Percent_Identity=33.9285714285714, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24639744, Length=280, Percent_Identity=33.9285714285714, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28571106, Length=280, Percent_Identity=33.9285714285714, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355903, Length=335, Percent_Identity=26.2686567164179, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35133; Mature: 35001

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEQAAEPALDVRRRLYELMTLMKAADDRLSKGIASGELQCVYWPSRGQEAIAAAVGVV
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
LRLDDQLVTTYRGLHDLIGKGVPLVEIYGEMLGRQVGSGRGKGGTMHIAHPDSGLMMSTG
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECC
IVGAGPPVAVGLAMAARRKGLDRVTVASFGDGATNTGSFHEAANMAALWDLPLVLVCQNN
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCHHHHCCCCEEEEECCC
QYAEMTPTGHTMKIAQVADRAAGYGMPGVRVDGNDPLAVVAALEEAVERARAGAGPTLLE
CEEEECCCCCEEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHH
CVTFRFRGHYFGDPMKYIPTEQMDAAIAADPLPRFRSRLLDDGICGARELDEIETAAVSA
HHHHHHCCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDEALAAVLAAPAAALDELDRDVYADPRNCPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTEQAAEPALDVRRRLYELMTLMKAADDRLSKGIASGELQCVYWPSRGQEAIAAAVGVV
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
LRLDDQLVTTYRGLHDLIGKGVPLVEIYGEMLGRQVGSGRGKGGTMHIAHPDSGLMMSTG
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECC
IVGAGPPVAVGLAMAARRKGLDRVTVASFGDGATNTGSFHEAANMAALWDLPLVLVCQNN
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCHHHHCCCCEEEEECCC
QYAEMTPTGHTMKIAQVADRAAGYGMPGVRVDGNDPLAVVAALEEAVERARAGAGPTLLE
CEEEECCCCCEEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHH
CVTFRFRGHYFGDPMKYIPTEQMDAAIAADPLPRFRSRLLDDGICGARELDEIETAAVSA
HHHHHHCCCCCCCCHHHCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDEALAAVLAAPAAALDELDRDVYADPRNCPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]