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The map label for this gene is paaF [C]

Identifier: 158314948

GI number: 158314948

Start: 3693795

End: 3694544

Strand: Direct

Name: paaF [C]

Synonym: Franean1_3139

Alternate gene names: 158314948

Gene position: 3693795-3694544 (Clockwise)

Preceding gene: 158314947

Following gene: 158314950

Centisome position: 41.12

GC content: 67.73

Gene sequence:

>750_bases
ATGACACTCGTCCGTTACGCCGTCGAGCGGGGGTGCGCCGTCATCACGCTCGACTCGCCCTGCAACCGCAATGCATTGTC
GGTCCAGCTCATGCAGGAGTTGTCCACCCACCTGACTACAGCTGCCGAGGCGGACGACATCCGGTGCGTCGTCCTCACCC
ACACGGGTTCGACCTTCTGCGCCGGGGGCGACCTGGCCGAGGGCGTCCCGGTGCGGAGTACGGAACGGTTTCGTGACCTC
CTGCGCCAAGTGGTCGAGCTACCCAAGCCGGTGCTGGGGCGTATCGACGGGCATGTTCGCGCCGCGGGCATCGGGTTGGT
GGGAGCGTGCGACATCGCCGTGGCGGGGCCGAGGACGACGTTCGCCTTCACCGAGGTCCGCATCGGCGTCGCGCCGGCGA
TCATCGCGCTGACGACACTGAGCCGGATGTCGGAACGTGCGGCTGCCCGGTACTGCCTGACAGGCGAGACCTTCGACGCG
ACGACGGCTGCGGCCACAGGGCTGGTCACTGTGGCAACACAGGACGTGGACGCCGAACTTGAGCGGATGCTGGAGGCGAT
CCGGGTCTGCTCGCCGCAGGGGCTCGCTGAGACCAAGCCGTTGACCACTCGAGGTGTCCTAGATGCGTTCGATGCCCGCG
CTGTAACGTTGCAGCAGCAGTCTGCGCGTCTCTTCGCCTCTGCGGAGGCGCAGGAGGGGATGGCCGCGTTCCTGGAGCGG
CGGCAGCCGCGTTGGGCTTTGCCTGCGTGA

Upstream 100 bases:

>100_bases
ACAGGCTGACCTGTCGGCCGCCAGCACGAGGAATGCATGACCGAGACAGGCGACATGGCGAGCACGACGCGAACCACACC
ACCAGCCGAAGGGAGAAGCC

Downstream 100 bases:

>100_bases
CGGTCGTTCGTGCGCCGCAGCCGCCGTGTACCTCCCATTCGGGCGGTTCCCGGCTCGGCTGAGTCGGTGGCGGGTGGTGA
GGCCGGACGAGGCGGGGATT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTLVRYAVERGCAVITLDSPCNRNALSVQLMQELSTHLTTAAEADDIRCVVLTHTGSTFCAGGDLAEGVPVRSTERFRDL
LRQVVELPKPVLGRIDGHVRAAGIGLVGACDIAVAGPRTTFAFTEVRIGVAPAIIALTTLSRMSERAAARYCLTGETFDA
TTAAATGLVTVATQDVDAELERMLEAIRVCSPQGLAETKPLTTRGVLDAFDARAVTLQQQSARLFASAEAQEGMAAFLER
RQPRWALPA

Sequences:

>Translated_249_residues
MTLVRYAVERGCAVITLDSPCNRNALSVQLMQELSTHLTTAAEADDIRCVVLTHTGSTFCAGGDLAEGVPVRSTERFRDL
LRQVVELPKPVLGRIDGHVRAAGIGLVGACDIAVAGPRTTFAFTEVRIGVAPAIIALTTLSRMSERAAARYCLTGETFDA
TTAAATGLVTVATQDVDAELERMLEAIRVCSPQGLAETKPLTTRGVLDAFDARAVTLQQQSARLFASAEAQEGMAAFLER
RQPRWALPA
>Mature_248_residues
TLVRYAVERGCAVITLDSPCNRNALSVQLMQELSTHLTTAAEADDIRCVVLTHTGSTFCAGGDLAEGVPVRSTERFRDLL
RQVVELPKPVLGRIDGHVRAAGIGLVGACDIAVAGPRTTFAFTEVRIGVAPAIIALTTLSRMSERAAARYCLTGETFDAT
TAAATGLVTVATQDVDAELERMLEAIRVCSPQGLAETKPLTTRGVLDAFDARAVTLQQQSARLFASAEAQEGMAAFLERR
QPRWALPA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=264, Percent_Identity=25.7575757575758, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=27.3469387755102, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=25.7936507936508, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI221136756, Length=239, Percent_Identity=23.4309623430962, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136753, Length=239, Percent_Identity=23.4309623430962, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=29.2682926829268, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082183, Length=246, Percent_Identity=26.0162601626016, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787660, Length=262, Percent_Identity=28.6259541984733, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=28.6821705426357, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=29.2490118577075, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=24.3589743589744, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=214, Percent_Identity=25.2336448598131, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=200, Percent_Identity=31, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=28.7128712871287, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=29.1338582677165, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=28.5140562248996, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=28.5140562248996, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=27.843137254902, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920382, Length=208, Percent_Identity=29.8076923076923, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 26556; Mature: 26425

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVRYAVERGCAVITLDSPCNRNALSVQLMQELSTHLTTAAEADDIRCVVLTHTGSTFC
CHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEE
AGGDLAEGVPVRSTERFRDLLRQVVELPKPVLGRIDGHVRAAGIGLVGACDIAVAGPRTT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCEEEEEEEEECCCCCE
FAFTEVRIGVAPAIIALTTLSRMSERAAARYCLTGETFDATTAAATGLVTVATQDVDAEL
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCEEEEEECCHHHHH
ERMLEAIRVCSPQGLAETKPLTTRGVLDAFDARAVTLQQQSARLFASAEAQEGMAAFLER
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
RQPRWALPA
CCCCCCCCC
>Mature Secondary Structure 
TLVRYAVERGCAVITLDSPCNRNALSVQLMQELSTHLTTAAEADDIRCVVLTHTGSTFC
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEE
AGGDLAEGVPVRSTERFRDLLRQVVELPKPVLGRIDGHVRAAGIGLVGACDIAVAGPRTT
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCEEEEEEEEECCCCCE
FAFTEVRIGVAPAIIALTTLSRMSERAAARYCLTGETFDATTAAATGLVTVATQDVDAEL
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHCEEEEEECCHHHHH
ERMLEAIRVCSPQGLAETKPLTTRGVLDAFDARAVTLQQQSARLFASAEAQEGMAAFLER
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
RQPRWALPA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA