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The map label for this gene is ykfC [H]

Identifier: 158314884

GI number: 158314884

Start: 3629141

End: 3629491

Strand: Direct

Name: ykfC [H]

Synonym: Franean1_3074

Alternate gene names: 158314884

Gene position: 3629141-3629491 (Clockwise)

Preceding gene: 158314882

Following gene: 158314885

Centisome position: 40.4

GC content: 63.53

Gene sequence:

>351_bases
GTGGCGGTACAGTTTGCCTGCACGCAGATCGGCAAGCCGTACGTGTGGGGCGGTAACGGTGACCCGGGATTCGACTGCTC
CGGCCTCACTTACGCTGCGTACGCCGCCGCCGGTATCGATATTCCACGGACAGCCCAAACCCAGCACGACGCCGGTCCGC
CGCTGCCGGCCGGAACGCCTCTTGAGCCAGGTGACCTGGTGTTCTTCGGCACCCAGAGGAACATCCATCATGTCGGGATC
TCCCTCGGAGGAACGATCATGATCGACGCACCTGATTTCGGAAGATCGGTGACGATCCGCGACTTCCAGGCCTTTCCCGA
CTATGCGGGTGCCTCCCGGCCGGCACAGTAG

Upstream 100 bases:

>100_bases
CACCCAGTACTCTGAACCGACGCCGAACGCTTCAGCAGCGTGCCCCACCCTTCAGTCAACAGTTCAGTCCCGAGCTGATC
ACTTCAGTAGTGCCGCGCTG

Downstream 100 bases:

>100_bases
TCGAGGCTTGGCGTGGTCTTTCCAGGCGTGCCGTGGCTGCCGACCCGACCGCAGTGATCCCGGCCCACGGTTCGCGGCGG
CGCCGTGTAGGACCCCTGGC

Product: NLP/P60 protein

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MAVQFACTQIGKPYVWGGNGDPGFDCSGLTYAAYAAAGIDIPRTAQTQHDAGPPLPAGTPLEPGDLVFFGTQRNIHHVGI
SLGGTIMIDAPDFGRSVTIRDFQAFPDYAGASRPAQ

Sequences:

>Translated_116_residues
MAVQFACTQIGKPYVWGGNGDPGFDCSGLTYAAYAAAGIDIPRTAQTQHDAGPPLPAGTPLEPGDLVFFGTQRNIHHVGI
SLGGTIMIDAPDFGRSVTIRDFQAFPDYAGASRPAQ
>Mature_115_residues
AVQFACTQIGKPYVWGGNGDPGFDCSGLTYAAYAAAGIDIPRTAQTQHDAGPPLPAGTPLEPGDLVFFGTQRNIHHVGIS
LGGTIMIDAPDFGRSVTIRDFQAFPDYAGASRPAQ

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=115, Percent_Identity=42.6086956521739, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1786421, Length=104, Percent_Identity=40.3846153846154, Blast_Score=69, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 12138; Mature: 12006

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVQFACTQIGKPYVWGGNGDPGFDCSGLTYAAYAAAGIDIPRTAQTQHDAGPPLPAGTP
CCEEEEEHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
LEPGDLVFFGTQRNIHHVGISLGGTIMIDAPDFGRSVTIRDFQAFPDYAGASRPAQ
CCCCCEEEECCCCCEEEEEEEECCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVQFACTQIGKPYVWGGNGDPGFDCSGLTYAAYAAAGIDIPRTAQTQHDAGPPLPAGTP
CEEEEEHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
LEPGDLVFFGTQRNIHHVGISLGGTIMIDAPDFGRSVTIRDFQAFPDYAGASRPAQ
CCCCCEEEECCCCCEEEEEEEECCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]